real-time PCR
High Resolution Melt (HRM) Software
Real-time PCR instruments
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• Minimal user input to achieve results • Easy recall analysis settings for future experiments • Greater accuracy and increased sensitivity • Ability to achieve more in one run • Shortcuts in experiment setup save time High resolution melt (HRM) analysis is an alternative to dHPLC sequencing screening of new gene variants. The Applied Biosystems HRM application does not require temperature shifting, which results in a greater likelihood of identifying new homozygous mutations than methods that require temperature shifting.
Product
Quantity
Cat. No.
High Resolution Melt (HRM) Software v3.0 for StepOne™, StepOnePlus™, and 7500 Systems
1 license 10 licenses
4461357 4461456
High Resolution Melt (HRM) Software v3.0 for ViiA™ 7 System
1 license
4453724
High Resolution Melt (HRM) Software v3.0 for 7900HT System
1 license
4397808
RealTime StatMiner® Software Advanced data mining software for real-time PCR data analysis RealTime StatMiner® Software provides a unique, straightforward solution to data analysis for real-time PCR for both life scientists and bioinformaticians. The intuitive user interface offers step-by-step guidance to simplify data processing and interpretation, helping researchers to extract biological relevance from real-time PCR data quickly and reliably. RealTime StatMiner® Software is widely used by pharmaceutical corporations, biotechnology companies, research centers, and core facilities around the world for high-throughput analysis of gene expression data. It combines commonly used interactive visualizations with advanced statistics to offer rapid, reliable analysis, allowing researchers to organize their samples based on technical and biological replicates, as well as rapidly create publication-ready reports. RealTime StatMiner® Software automatically detects outliers and filters low-expressed or undetermined detectors, alerting the user to problems within their data and logging all activities during analysis. It also offers the unique ability to compute ∆Ct-based multiple endogenous controls, automatically determining the most stable controls using algorithms such Product
as geNorm, NormFinder, and Minimum Variance Median. RealTime StatMiner® Software is available as a standalone as well as a TIBCO® Spotfire® software compatible plug-in that can be integrated with Integromics Biomarker Discovery® and SeqSolve™ software to provide a complete solution for gene expression analysis. Compatibility
• Multi-platform compatibility—co-developed with Applied Biosystems for use with OpenArray®, ViiA™ 7, 7900HT, 7500, and 7500 Fast, StepOne™, StepOnePlus™, and other systems • Multiple experimental assays—including TaqMan® and SYBR® Green • Universal Data Loader—simplifying use of nonstandard data formats • High throughput—allows use of multiple plates and samples, with more than 10 plates per run
Quantity
Cat. No.
RealTime StatMiner Software (stand-alone & TIBCO Spotfire software) for Academic Use
1-yr license 3-yr license
4398545 4398087
RealTime StatMiner® Software (stand-alone & TIBCO®Spotfire® software) for Commercial Use
1-yr license 3-yr license
4398532 4398086
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High Resolution Melt Software v3.0 is a new and improved tool for HRM analysis. HRM analysis is a post-PCR analysis method used to identify variation in nucleic acid sequences. The method is based on detecting small differences in PCR melt (dissociation) curves. It is enabled by high-brightness dsDNA-binding dyes used in conjunction with real-time PCR instrumentation that has precise temperature ramp control, advanced data capture capabilities, and access to software designed specifically for HRM analysis. High Resolution Melt Software v3.0 brings HRM to the StepOne™ and StepOnePlus™ RealTime PCR Systems, as well as the 7500 Fast Real-Time PCR System, and contains features that make fast and accurate HRM results more accessible than ever.
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For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use. © 2011 Life Technologies Corporation. All rights reserved. The trademarks mentioned herein are the property of Life Technologies Corporation or their respective owners.