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May 2012
feature table 1
uBcc ha sample
ph of a 0.1% aqueus solution
6-8
water content (KF)
<10%
residue on ignition
7-10%
intrinsic viscosity @ 25oc
2-3.5 m3/kg
protein content by Lowry
<0.1%
Glucuronic acid (dry basis)
45.0-48.4%
sodium hyaluronate (dry basis) total aerobic plate count
<100 cFu/g
nonconforming organisms
satisfy the need of oxygen consumption by fermenting microorganisms, minimizing the shear stress on fermenting cells. With an optimal dissolved oxygen level in the medium, stirring speed in fermentation can be lowered reducing damage to the cells. The fermentation time can then be shortened, improving unit time yield. At the same time a variable oxygen level can be tightly controlled, which greatly improves batch-to-batch consistency. The advancement of the new HA fermentation system can provide the following beneďŹ ts to HA production: (i) increase productivity: high batch-to-batch consistency in production; higher unit yield; short fermenting time. (ii) improvement in quality: customizable molecular weight in low polydispersity per batch; toxin free high purity; large molecular size and stable structure, being stable in exposure to higher temperature and other autoclave processes that satisfy the need of emerging medical applications. The value from the addition of the large HA for producing HA-based secondary products could be: (i) the large HA product has superior mechanical, chemical and heat stability suitable for more stringent sterilization and
>99% none recovered
other processes in the production of HA-based materials. (ii) it also offers improved viscosity due to higher molecular weight retention when compared to Streptococcus-derived HA after autoclaved. (iii) less consumption, therefore time and cost savings in production of HA-based materials and increased facility throughput. (iv) the high purity of HA as a raw material delivers highly consistent material that can translate into more consistent product formulations and improved performance in HA-based final product. (v) Extending storage time of HA-based products.
weight properties of hyaluronic acid produced by Streptococcus zooepidemicus. Jl. Environ. Microbiol. 1997. 63: 27592764. 5. Duan XJ., Yang L., Zhang X., Tan WS.: Effects of oxygen and shear stress on molecular weight of hyaluronic acid produced by Streptococcus zooepidemicus. Appl. Microbiol. Biotechnol. 2008. 18: 718-724. 6. Chen X., Marcellin E., Hung J., Nielsen LK.:
Hyaluronan molecular weight is controlled by UDP-N-acetylglucosamine concentration in Streptococcus zooepidemicus. J. Biol. Chem. 2009, 284: 18007-18014. 7. Jagnaath S., Ramachandran K.,: Influence of competing metabolic processes on the molecular weight of hyaluronic acid synthesized by Streptococcus zooepidemicus. Biochem. Eng. J. 2010, 48: 148-158.
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THE 13th INTERNATIONAL CONFERENCE ON SYSTEMS BIOLOGY
Reference 1. Sheng JZ., Ling PX., et al.,: Use of induction promoters to regulate hyaluronan synthase and UDP-glucose-6-dehydrogenase of Streptococcus zooepidemicus expression in locyococcus lactis: a case study of the regulation mechanism of hyaluronic acid polymer. J. Appl. Microbiol. 2009, 107, 136-144. 2. Biomaterial Market Report 2010, Frost and Sullivan. 3. Weigel PH., Hascall VC., Tammi M.: Hyaluronan Synthase. J. Biol.Chem., 272, 13997-14000, 1997 4. Armstrong DC., Johns MR: Culture conditions affect the molecular
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