Phenotyping Banana Germplasm with Fusarium oxysporum f.sp. cubense, Tropical Race 4

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PHENOTYPING BANANA GERMPLASM

The Interdisciplinary Research and Education Program of Wageningen University (INREF) funds the research program “Panama disease in banana: multilevel solutions for a global problem�. The program is a public private partnership through co-funding by many partners and comprises seven PhD projects.


PHENOTYPING BANANA GERMPLASM

The project of Fernando Garcia focuses on “The genetic basis and mechanisms of resistance to Panama disease in banana”. Part of the project involves the screening of banana germplasm with the Tropical Race 4 strain of Fusarium oxysporum f.sp. cubense, the causal agent of Panama disease. In this database we present a short summary of collected data that will be frequently updated in order to inform the community. Details of these experiments will eventually be published in peer-reviewed journals. Data can be used with correct citation of the source of information. The following plant information (all available passport data and biological information) was obtained from the following website: http://www.ars-grin.gov

1

García-Bastidas, F., and Kema, G.H.J., (2014, Oct 15). " USDA Banana germplasm phenotyping with Fusarium oxysporum f.sp. cubense Tropical Race 4. Retrieved from http://panamadisease.org/en /research


ZIV TARS #

17169

Accession name

Ziv

Group

Cavendish

Ploidy/Genome

3X/AAA

Microscopy

AAA

Acquisition date Donor country

Puerto Rico/USDA

Testing date

10-7-2014 to 20-8-2014

Left: Healthy plant and corm (water control); middle and right: two infected plants with FocTR4

2


WILLIAMS TARS #

17414

Accession name

Williams

Group

Cavendish

Ploidy/Genome

3X/AAA

Microscopy

AAA

Acquisition date Donor country

Puerto Rico/USDA

Testing date

10-7-2014 to 20-8-2014

Left: Healthy plant and corm (water control); middle and right: two infected plants with FocTR4

3


VOLUNTEER MUSA TARS #

17423

Accession name

Volunter Musa

Group

Cavendish

Ploidy/Genome

3X/AAA

Microscopy

AAA

Acquisition date Donor country

Puerto Rico/USDA

Testing date

10-7-2014 to 20-8-2014

Left: Healthy plant and corm (water control); middle and right: two infected plants with FocTR4

4


VERDIN TARS #

17150

Accession name

Verdin

Group

Red

Ploidy/Genome

3X/AAA

Microscopy

AAA

Acquisition date Donor country

Puerto Rico/USDA

Testing date

10-7-2014 to 20-8-2014

Left: Healthy plant and corm (water control); middle and right: two infected plants with FocTR4

5


VALERY TARS #

17158

Accession name

Valery

Group

Cavendish

Ploidy/Genome

3X/AAA

Microscopy

AAA

Acquisition date Donor country

Puerto Rico/USDA

Testing date

10-7-2014 to 20-8-2014

Left: Healthy plant and corm (water control); middle and right: two infected plants with FocTR4

6


TUU GIA TARS #

17382

Accession name

Tuu Gia

Group

Cultivated diploid

Ploidy/Genome

2X/AA

Microscopy

AA

Acquisition date Donor country

Puerto Rico/USDA

Testing date

10-7-2014 to 20-8-2014

Left: Healthy plant and corm (water control); middle and right: two infected plants with FocTR4

7


TMB2X 9128-3 TARS #

18049

Accession name

TMB2X 9128-3

Group

Synthetic hybrid

Ploidy/Genome

-

Microscopy

AA

Acquisition date Donor country

Puerto Rico/USDA

Testing date

10-7-2014 to 20-8-2014

Left: Healthy plant and corm (water control); middle and right: two infected plants with FocTR4

8


TIGUA TARS #

17407

Accession name

Tigua (PNG-625)

Group

Iholena

Ploidy/Genome

3X/AAB

Microscopy

AAB

Acquisition date Donor country

Puerto Rico/USDA

Testing date

10-7-2014 to 20-8-2014

Left: Healthy plant and corm (water control); middle and right: two infected plants with FocTR4

9


THOUSAND FINGERS TARS #

17992

Accession name

Thousand Fingers

Group

Pisang Seribu

Ploidy/Genome

3X/AAB

Microscopy

AAA††

††

Acquisition date Donor country

Puerto Rico/USDA

Testing date

10-7-2014 to 20-8-2014

†† Ploidy and/or genomic composition changed from previously reported

Left: Healthy plant and corm (water control); middle and right: two infected plants with FocTR4

10


TAIWANESE SOMACLONE TARS #

17395

Accession name

Taiwanese Somaclone

Group

Cavendish

Ploidy/Genome

3X/AAA

Microscopy

AAA

Acquisition date Donor country

Puerto Rico/USDA

Testing date

10-7-2014 to 20-8-2014

Left: Healthy plant and corm (water control); middle and right: two infected plants with FocTR4

11


PRAYING HANDS TARS #

17173

Accession name

Praying hands

Group

Saba

Ploidy/Genome

3X/ABB

Microscopy

ABB

Acquisition date Donor country

Puerto Rico/USDA

Testing date

10-7-2014 to 20-8-2014

Left: Healthy plant and corm (water control); middle and right: two infected plants with FocTR4

12


NIÑO COMÚN TARS #

17379

Accession name

Niño Común

Group

Sucrier

Ploidy/Genome

2X/AA

Microscopy

AA

Acquisition date Donor country

Puerto Rico/USDA

Testing date

10-7-2014 to 20-8-2014

Left: Healthy plant and corm (water control); middle and right: two infected plants with FocTR4

13


MORADO ENANO TARS #

17425

Accession name

Morado Enano

Group

Red

Ploidy/Genome

3X/AAA

Microscopy

AAA

Acquisition date Donor country

Puerto Rico/USDA

Testing date

10-7-2014 to 20-8-2014

Left: Healthy plant and corm (water control); middle and right: two infected plants with FocTR4

14


MONTECRISTO ENANO TARS #

17428

Accession name

Montecristo Enano

Group

Cavendish

Ploidy/Genome

3X/AAA

Microscopy

AAA

Acquisition date Donor country

Puerto Rico/USDA

Testing date

10-7-2014 to 20-8-2014

Left: Healthy plant and corm (water control); middle and right: two infected plants with FocTR4

15


MANZANO TARS #

17136

Accession name

Manzano

Group

Silk

Ploidy/Genome

3X/AAB

Microscopy

AAB

Acquisition date Donor country

Puerto Rico/USDA

Testing date

10-7-2014 to 20-8-2014

Left: Healthy plant and corm (water control); middle and right: two infected plants with FocTR4

16


LACATAN TARS #

17144

Accession name

Lacatan#

Group

Gros Michel

Ploidy/Genome

3X/AAA

Microscopy

AAA

Acquisition date Donor country

Puerto Rico/USDA

Testing date

10-7-2014 to 20-8-2014

# Accession is incorrect based on microsatellite profile and general clustering

Left: Healthy plant and corm (water control); middle and right: two infected plants with FocTR4

17


JOHNSON TARS #

18061

Accession name

Johnson

Group

Cavendish

Ploidy/Genome

3X/AAA

Microscopy

AAA

Acquisition date Donor country

Puerto Rico/USDA

Testing date

10-7-2014 to 20-8-2014

Left: Healthy plant and corm (water control); right: infected plant with FocTR4

18


IGCPOCA TARS #

17396

Accession name

Igcpoca

Group

Mutika/Lujugira

Ploidy/Genome

-

Microscopy

AAA

Acquisition date Donor country

Puerto Rico/USDA

Testing date

10-7-2014 to 20-8-2014

Left: Healthy plant and corm (water control); right: infected plant with FocTR4

19


HIGHGATE TARS #

17153

Accession name

Highgate#

Group

Cavendish

Ploidy/Genome

3X/AAA

Microscopy

AAA

Acquisition date Donor country

Puerto Rico/USDA

Testing date

10-7-2014 to 20-8-2014

# Accession is incorrect based on microsatellite profile and general clustering

Left: Healthy plant and corm (water control); right: infected plant with FocTR4

20


GUINEO ENANO TARS #

17177

Accession name

Guineo Enano

Group

Cavendish

Ploidy/Genome

3X/AAA

Microscopy

AAA

Acquisition date Donor country

Puerto Rico/USDA

Testing date

10-7-2014 to 20-8-2014

Left: Healthy plant and corm (water control); middle and right: two infected plants with FocTR4

21


GURARร N ENANO TARS #

17840

Accession name

Guarรกn Enano

Group

Gross Michel

Ploidy/Genome

3X/AAA

Microscopy

AAA

Acquisition date Donor country

Puerto Rico/USDA

Testing date

10-7-2014 to 20-8-2014

Left: Healthy plant and corm (water control); middle and right: two infected plants with FocTR4

22


GIANT GOVERNOR TARS #

17163

Accession name

Giant Governor

Group

Cavendish

Ploidy/Genome

3X/AAA

Microscopy

AAA

Acquisition date Donor country

Puerto Rico/USDA

Testing date

10-7-2014 to 20-8-2014

Left: Healthy plant and corm (water control); middle and right: two infected plants with FocTR4

23


FALSE HORN DOMINICAN RED TARS #

17813

Accession name

False Horn Dominican Red

Group

Plantain (False-Horn)

Ploidy/Genome

3X/AAB

Microscopy

AAB

Acquisition date Donor country

Puerto Rico/USDA

Testing date

10-7-2014 to 20-8-2014

Left: Healthy plant and corm (water control); middle and right: two infected plants with FocTR4

24


ENANO GIGANTE TARS #

17143

Accession name

Enano Gigante

Group

Cavendish

Ploidy/Genome

3X/AAA

Microscopy

AAA

Acquisition date Donor country

Puerto Rico/USDA

Testing date

10-7-2014 to 20-8-2014

Left: Healthy plant and corm (water control); middle and right: two infected plants with FocTR4

25


DWARF VALERY TARS #

17402

Accession name

Dwarf Valery

Group

Cavendish

Ploidy/Genome

3X/AAA

Microscopy

AAA

Acquisition date Donor country

Puerto Rico/USDA

Testing date

10-7-2014 to 20-8-2014

Left: Healthy plant and corm (water control); middle and right: two infected plants with FocTR4

26


CUBAN RED TARS #

17392

Accession name

Cuban Red

Group

Red

Ploidy/Genome

3X/AAA

Microscopy

AAA

Acquisition date Donor country

Puerto Rico/USDA

Testing date

10-7-2014 to 20-8-2014

Left: Healthy plant and corm (water control); middle and right: two infected plants with FocTR4

27


CRBP 39 TARS #

18056

Accession name

CRBP 39

Group

Synthetic Hybrid

Ploidy/Genome

4X/AAAB

Microscopy

AAAB

Acquisition date Donor country

Puerto Rico/USDA

Testing date

10-7-2014 to 20-8-2014

Left: Healthy plant and corm (water control); middle and right: two infected plants with FocTR4

28


10-A

Left: Healthy plant and corm (water control); middle and right: two infected plants with FocTR4

29


8-A

TARS #

17159

Accession name

8-A

Group

Cavendish

Ploidy/Genome

3X/AAA

Microscopy

AAA

Acquisition date Donor country

Puerto Rico/USDA

Testing date

10-7-2014 to 20-8-2014

Left: Healthy plant and corm (water control); middle and right: two infected plants with FocTR4

30


6-A TARS #

17130

Accession name

6-A

Group

Cavendish

Ploidy/Genome

3X/AAA

Microscopy

AAA

Acquisition date Donor country

Puerto Rico/USDA

Testing date

10-7-2014 to 20-8-2014

Left: Healthy plant and corm (water control); middle and right: two infected plants with FocTR4

31


5-A TARS #

17135

Accession name

5-A

Group

Cavendish

Ploidy/Genome

3X/AAA

Microscopy

AAA

Acquisition date Donor country

Puerto Rico/USDA

Testing date

10-7-2014 to 20-8-2014

Left: Healthy plant and corm (water control); middle and right: two infected plants with FocTR4

32


4-R-2,500 TARS #

17151

Accession name

4-R-2,500

Group

Gros Michel

Ploidy/Genome

3X/AAA

Microscopy

AAA

Acquisition date Donor country

Puerto Rico/USDA

Testing date

10-7-2014 to 20-8-2014

Left: Healthy plant and corm (water control); middle and right: two infected plants with FocTR4

33


3-R-2,500 TARS #

17141

Accession name

3-R-2,500

Group

Gros Michel

Ploidy/Genome

3X/AAA

Microscopy

AAA

Acquisition date Donor country

Puerto Rico/USDA

Testing date

10-7-2014 to 20-8-2014

Left: Healthy plant and corm (water control); middle and right: two infected plants with FocTR4

34


2-A TARS #

17125

Accession name

2-A

Group

Cavendish

Ploidy/Genome

3X/AAA

Microscopy

AAA

Acquisition date Donor country

Puerto Rico/USDA

Testing date

10-7-2014 to 20-8-2014

Left: Healthy plant and corm (water control); middle and right: two infected plants with FocTR4

35


1-A TARS #

17164

Accession name

1-A

Group

Cavendish

Ploidy/Genome

3X/AAA

Microscopy

AAA

Acquisition date Donor country

Puerto Rico/USDA

Testing date

10-7-2014 to 20-8-2014

Left: Healthy plant and corm (water control); middle and right: two infected plants with FocTR4

36


SECOND BATCH 3-A TARS #

17154

Accession name

3-A

Group

Cavendish

Ploidy/Genome

3X/AAA

Microscopy

AAA

Acquisition date Donor country

Puerto Rico/USDA

Testing date

10-7-2014 to 20-8-2014

Left: Healthy plant and corm (water control); middle and right: two infected plants with FocTR4

37


9-A TARS #

17167

Accession name

9-A#

Group

Cavendish

Ploidy/Genome

3X/AAA

Microscopy

AAA

Acquisition date Donor country

Puerto Rico/USDA

Testing date

10-7-2014 to 20-8-2014

# Accession is incorrect based on microsatellite profile and general clustering

Left: Healthy plant and corm (water control); middle and right: two infected plants with FocTR4

38


2390 TARS #

17165

Accession name

2390

Group

Synthetic Hybrid

Ploidy/Genome

4X/AAAA

Microscopy

AAAA

Acquisition date Donor country

Puerto Rico/USDA

Testing date

10-7-2014 to 20-8-2014

Left: Healthy plant and corm (water control); middle and right: two infected plants with FocTR4

39


BLUE TORRES STRAIGHT ISLAND TARS #

18018

Accession name

Blue Torres Straight Island

Group

Ney Mannan

Ploidy/Genome

3X/ABB

Microscopy

ABB

Acquisition date Donor country

Puerto Rico/USDA

Testing date

10-7-2014 to 20-8-2014

Left: Healthy plant and corm (water control); middle and right: two infected plants with FocTR4

40


CACAMBOU TARS #

18020

Accession name

Cacambou

Group

Bluggoe

Ploidy/Genome

3X/ABB

Microscopy

ABB

Acquisition date Donor country

Puerto Rico/USDA

Testing date

10-7-2014 to 20-8-2014

Left: Healthy plant and corm (water control); middle and right: two infected plants with FocTR4

41


CARDABA HONDURAS TARS #

17822

Accession name

Cardaba Honduras

Group

Saba

Ploidy/Genome

3X/ABB

Microscopy

ABB

Acquisition date Donor country

Puerto Rico/USDA

Testing date

10-7-2014 to 20-8-2014

Left: Healthy plant and corm (water control); middle and right: two infected plants with FocTR4

42


CONGO 300 TARS #

16513

Accession name

Congo 300

Group

Plantain (False Horn)

Ploidy/Genome

3X/AAB

Microscopy

AAB

Acquisition date Donor country

Puerto Rico/USDA

Testing date

10-7-2014 to 20-8-2014

Left: Healthy plant and corm (water control); middle and right: two infected plants with FocTR4

43


DWARF ORINOCO TARS #

17397

Accession name

Dwarf Orinoco

Group

Bluggoe

Ploidy/Genome

3X/ABB

Microscopy

ABB

Acquisition date Donor country

Puerto Rico/USDA

Testing date

10-7-2014 to 20-8-2014

Left: Healthy plant and corm (water control); middle and right: two infected plants with FocTR4

44


DWARF SUPERPLANTAIN TARS #

16507

Accession name

Dwarf Superplantain

Group

Plantain (French)

Ploidy/Genome

3X/AAB

Microscopy

AAB

Acquisition date Donor country

Puerto Rico/USDA

Testing date

10-7-2014 to 20-8-2014

Left: Healthy plant and corm (water control); middle and right: two infected plants with FocTR4

45


FHIA-17 TARS #

18058

Accession name

FHIA-17

Group

Synthetic Hybrid

Ploidy/Genome

4X/AAAA

Microscopy

AAAA

Acquisition date Donor country

Puerto Rico/USDA

Testing date

10-7-2014 to 20-8-2014

Left: Healthy plant and corm (water control); middle and right: two infected plants with FocTR4

46


FRENCH TYPE DOMINICAN TARS #

17816

Accession name

French Type Dominican

Group

Plantain (French)

Ploidy/Genome

3X/AAB

Microscopy

AAB

Acquisition date Donor country

Puerto Rico/USDA

Testing date

10-7-2014 to 20-8-2014

Left: Healthy plant and corm (water control); middle and right: two infected plants with FocTR4

47


GIPUNGUSI TARS #

18023

Accession name

Gipungusi

Group

Bluggoe

Ploidy/Genome

3X/ABB

Microscopy

ABB

Acquisition date Donor country

Puerto Rico/USDA

Testing date

10-7-2014 to 20-8-2014

Left: Healthy plant and corm (water control); middle and right: two infected plants with FocTR4

48


HUA MOA TARS #

17824

Accession name

Hua Moa Popoulu

Group

Maia Maoli Popoulu

Ploidy/Genome

3X/AAB

Microscopy

AAB

Acquisition date Donor country

Puerto Rico/USDA

Testing date

10-7-2014 to 20-8-2014

Left: Healthy plant and corm (water control); middle and right: two infected plants with FocTR4

49


HY BRAZILIAN TARS #

17378

Accession name

Hy Brazilian

Group

Pome – Tall

Ploidy/Genome

3X/AAB

Microscopy

AAB

Acquisition date Donor country

Puerto Rico/USDA

Testing date

10-7-2014 to 20-8-2014

Left: Healthy plant and corm (water control); middle and right: two infected plants with FocTR4

50


IGNAMICO TARS #

17409

Accession name

Ignamico

Group

Mutika/Lujugira

Ploidy/Genome

3X/AAA

Microscopy

AAA

Acquisition date Donor country

Puerto Rico/USDA

Testing date

10-7-2014 to 20-8-2014

Left: Healthy plant and corm (water control); middle and right: two infected plants with FocTR4

51


KATUAI VUNALAIR TARS #

17412

Accession name

Katual Vunalair

Group

Cultivated diploid

Ploidy/Genome

2X/AA

Microscopy

AA

Acquisition date Donor country

Puerto Rico/USDA

Testing date

10-7-2014 to 20-8-2014

Left: Healthy plant and corm (water control); middle and right: two infected plants with FocTR4

52


LACKNAU P.I.23472 TARS #

16516

Accession name

Lacknau P.I.23472

Group

Laknao

Ploidy/Genome

3X/AAB

Microscopy

AAB

Acquisition date Donor country

Puerto Rico/USDA

Testing date

10-7-2014 to 20-8-2014

Left: Healthy plant and corm (water control); middle and right: two infected plants with FocTR4

53


MONTHAN TARS #

18024

Accession name

Monthan (MIA 34906)

Group

Monthan

Ploidy/Genome

-

Microscopy

ABB

Acquisition date Donor country

Puerto Rico/USDA

Testing date

10-7-2014 to 20-8-2014

Left: Healthy plant and corm (water control); middle and right: two infected plants with FocTR4

54


NCHUMBAHAKA TARS #

17408

Accession name

Nchumbahaka

Group

Mutika/Lujugira

Ploidy/Genome

3X/AAA

Microscopy

AAA

Acquisition date Donor country

Puerto Rico/USDA

Testing date

10-7-2014 to 20-8-2014

Left: Healthy plant and corm (water control); right: infected plant with FocTR4

55


PA 03-22 TARS #

18050

Accession name

PA 03-02#

Group

Synthetic Hybrid

Ploidy/Genome

4X/AAAB

Microscopy

AA

††

††

Acquisition date Donor country

Puerto Rico/USDA

Testing date

10-7-2014 to 20-8-2014

# Accession is incorrect based on microsatellite profile and general clustering †† Ploidy and/or genomic composition changed from previously reported

Left: Healthy plant and corm (water control); middle and right: two infected plants with FocTR4

56


PELIPITA COSTA RICA TARS #

17818

Accession name

Pelipita – Costa Rica

Group

Pelipita

Ploidy/Genome

3X/ABB

Microscopy

ABB

Acquisition date Donor country

Puerto Rico/USDA

Testing date

10-7-2014 to 20-8-2014

Left: Healthy plant and corm (water control); middle and right: two infected plants with FocTR4

57


PV 42-81 TARS #

18054

Accession name

PV 42-81

Group

Synthetic Hybrid

Ploidy/Genome

4X/AAAB

Microscopy

AAAB

Acquisition date Donor country

Puerto Rico/USDA

Testing date

10-7-2014 to 20-8-2014

Left: Healthy plant and corm (water control); middle and right: two infected plants with FocTR4

58


PV 42-320 TARS #

18055

Accession name

PV 42-320

Group

Synthetic Hybrid

Ploidy/Genome

4X/AAAB

Microscopy

AAAB

Acquisition date Donor country

Puerto Rico/USDA

Testing date

10-7-2014 to 20-8-2014

Left: Healthy plant and corm (water control); middle and right: two infected plants with FocTR4

59


RAJAPURI TARS #

16522

Accession name

Rajapuri

Group

Pome – Short

Ploidy/Genome

3X/AAB

Microscopy

AAB

Acquisition date Donor country

Puerto Rico/USDA

Testing date

10-7-2014 to 20-8-2014

Left: Healthy plant and corm (water control); middle and right: two infected plants with FocTR4

60


SEÑORITA TARS #

17410

Accession name

Señorita

Group

Pome – Tall

Ploidy/Genome

3X/AAB

Microscopy

AAB

Acquisition date Donor country

Puerto Rico/USDA

Testing date

10-7-2014 to 20-8-2014

Left: Healthy plant and corm (water control); middle and right: two infected plants with FocTR4

61


SH-3640 TARS #

08047

Accession name

SH-3640

Group

Synthetic Hybrid

Ploidy/Genome

3X/AAB

Microscopy

AAB

Acquisition date Donor country

Puerto Rico/USDA

Testing date

10-7-2014 to 20-8-2014

Left: Healthy plant and corm (water control); middle and right: two infected plants with FocTR4

62


TMP X 1621-1 TARS # Accession name

TMP x1621-1

Group

Synthetic Hybrid

Ploidy/Genome

4X/AAAB

Microscopy

AAAB

Acquisition date Donor country

Puerto Rico/USDA

Testing date

10-7-2014 to 20-8-2014

Left: Healthy plant and corm (water control); right: infected plant with FocTR4

63


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