Going Viral glowing in the darkness like an island seen from on high, a slice of the brain of a mouse shows blue neurons hugging its coastline and green neurons traversing the interior. This is a sliver of the cerebellum, the part of the brain where motor control resides. It is an image with quiet beauty, but it is also an image that tells a story about the connections neurons make—a story that may have surprising implications for human health. Scientists in the Department of Neurobiology at HMS are mapping the landscape between neurons to gain a deeper understanding of brain function. They are tracking how neurons communicate with one another by following the trail of chemical or electrical signals these cells send across specialized structures called synapses. 44 harvard medicine ~ winter 2013
New technologies in neuroimaging are making it easier to see neurons and neural paths. Some of the neurons in this mouse cerebellum, for example, were infected with a virus engineered to produce a green fluorescent protein. Once this virus entered the neuron, it replicated and traveled along the neuron and dendrites to the synapses, where it jumped to connecting neurons. Other neurons were stained with blue dye, allowing the researchers to see the brain’s different structures, thus providing a sort of cellular topography against which to reference the movements of the fluorescing virus. The researchers then used a fluorescent microscope to find the traveling viruses and to observe the dispersal of green to pinpoint synaptic connections and see how information flows through the brain.
