New Sixth Edition Updated Genomics Chapter 9, DNA-Based Information Technologies, now incorporates the latest genomic methods, such as: Next generation DNA sequencing, including the Illumina and 454 sequencing methods and platforms
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n Blood coagulation pathways in the context of enzymatic regulation (Chapter 6) n
Asymmetric lipid distribution in bilayer (Chapter 11)
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Role of BAR superfamily proteins in membrane curvature (Chapter 11)
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Applications of genomics, including the use of haplotypes to trace human migrations and phylogenetics to locate human genes associated with inherited disease conditions n
Forensic genotyping and the use of personalized genomics in medicine Numerous other chapters have been updated to reflect advances gained from new genomic methods. n
Next generation reversible terminator sequencing. F I G U RE 9 - 2 6
Transition state analogs and irreversible inhibition (Chapter 6)
Scaffold proteins (AKAPS and others) and their regulatory roles (Chapter 12) n
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Reactive oxygen species as by-products and as signals (Chapter 19)
Structure and function of the oxygen-evolving metal cluster in PSII (Chapter 19)
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n Formation, transport of lipoproteins in mammals, including the roles of
SREBP, SCAP, Insig in cholesterol regulation (Chapter 21) Integration of carbohydrate and lipid metabolism by PPARs, SREBPs, mTORC1, and LXR (Chapters 21, 23) n
n Creatine phosphate and the role of creatine kinase in moving ATP to cytosol (Chapter 23) n Microbial symbionts in the gut and their influence on energy metabolism and adipogenesis (Chapter 23) n Nucleosomes: their modification and positioning and higher order chromatin structure (Chapter 24) n
DNA polymerases and homologous recombination (Chapter 25)
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Loading eukaryotic RNA polymerase II (Chapter 26)
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Mutation-resistant nature of the genetic code (Chapter 27)
n Ribosome structure and the initiation of translation in eukaryotes (Chapter 27) n DNA looping, combinatorial control, chromatin remodeling, and positive
regulation in eukaryotes (Chapter 28)
New Science Every chapter has been thoroughly revised and updated to include both the most important advances in biochemistry and information needed in a modern biochemistry text. Among the new and/or thoroughly updated topics in this edition are:
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Regulation of the initiation of transcription in eukaryotes (Chapter 28)
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Steroid-binding nuclear receptors (Chapter 28)
New Biochemical Methods
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Prebiotic evolution, black smokers, and the RNA world (Chapter 1)
New methods or updates described in this edition include:
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Intrinsically disordered proteins (Chapter 4)
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Binding of the intrinsically disordered carboxyl terminus of p53 protein to its binding partners. F I G U RE 4 - 2 4
Modern Sanger protein sequencing and mass spectrometry (Chapter 3)
n Mass spectrometry applied to proteomics, glycomics, lipidomics, and metabolomics (Chapters 3, 7, 10) n Oligosaccharide microarrays to explore protein-oligosaccharide interactions and the “carbohydrate code� (Chapter 7)
n Modern genomic methods, including next-generation DNA sequencing (Chapter 9) n The use of genomics to locate genes involved in human disease (Chapter 9) n
Quantitative PCR and reverse transcriptase PCR (Chapter 9)
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Genetic engineering of photosynthetic organisms (Chapter 20)
n Use of Positron Emission Tomography (PET) to visualize tumors and brown adipose tissue (Chapter 23) n Development of bacterial strains with altered genetic codes for site-specific insertion of novel amino acids into proteins (Chapter 27)