enzyme experiment

EnzymeCatalaseExperiment

Introduction:Withinacell,enzymesareusedasacatalysttoincreasetherateofchemicalreactionTheydonot consumethemselves,rathertheyhelpinincreasingtherateofreaction.Withinthebody,enzymesvarydepending ontheirspecificfunctionsForinstance,hydrogenperoxideisatoxicchemical,butitbreaksdownintoharmless oxygenandwater.Thisreactioncanbespedupusingtheenzymecatalystproducedbyyeast.Hydrogenperoxide isproducedasabyproductincellularreaction...

TeamEnzymeExperiment

TeamenzymeprojectreportIntroductionEnzymesareproteinsthatactsasbiologicalcatalysts,helpingtocatalyze chemicalreactions.Thisismeansthatanalternativereactionpathwayofloweractivationenergyisprovided duringthespeedupreactionMostenzymesareproteinsandhencehaveathree-dimensionalstructureoffolded polypeptidechains.MostenzymeshaveanoptimaltemperatureandpHrangeinwhichtheyfunctionmost effectivelyIfchangesoccurthatremovetheenzymeoutofthis

LabExperimentOnTheEffectOfTemperatureOnEnzymes

Title:EffectsofTemperatureonEnzymesProblem/Purpose:Howdoestemperatureaffecttherateofenzyme activityonitssubstrate?Hypothesis:Ifthetemperatureincreases,thenrateofthechemicalreactionwillincrease aswell,andifthetemperaturedecreases,thentherateofreactionwillalsodecreaseData:Therewerethreetest tubesinwhichtheexperimentwasheld.Arelativelyequalsizedportionofrawpotato(thiscontainedtheenzyme [abiologicalcatalyst]hydrogenperoxidase)was

Biology:Experiment-TheEffectofTemperatureontheEnzyme Rennin

Aim:Theaimoftheexperimentistotesttheeffecttemperaturehasontheactivityoftheenzymerennin. Hypothesis:Ibelievetherateofreactionwillspeedupasthetemperatureincreasesuntilitreachesabout37oC, whichisthebodytemperature,whereitwillbegintoslowdownandstopreacting.Ibelievethiswilloccur becauseenzymeshaveatemperaturerangeatwhichtheyworkbestinandoncethetemperaturegoesoutofthis rangetheenzymewillstopworking.Introduction:Enzymesare...

InvestigatingTheRateOfEnzymeActivityWithinAExperimentEssay

Thepurposeofthisreportistodeterminewhichcatalysteffecttherateofenzymeactivitywithinaexperiment. Enzymeslowertheenergyofactivationbybindingwithsubstrateinchemicalreactionstoallowthereactionto occur.energyactivationisthereguiredamountofenergyrequiredamountofenergyneededtostartachemical reactionThisdecreasesthefastertheenzymereactiongoesbytheshapechangeonanactivesitetheconclusion isthattheenviromentalconditionssuchaspH,temperature...

ExplainHowCouldYouTestTheHypothesisThatAnEnzymeWas CompletelyDepletedDuringAnExperiment

ifanenzymewascompletelydepletedduringanexperiment?Myunderstandingisthatenzymeshavethecapacity ofaccelerationabiochemicalreactionwithoutbeingconsumedordepleted(Santhosh,2017).However,apossible waytodetectiftheenzymeisstillreactingisbyseeingifthereactionisstilloccurring.Forinstance,inexperiment two,afteraboutaminute,thereactionstoppedbubblingandproducinggases2Describeanexperimentthatcould testthehypothesisthatanenzymebinds...

ComparingFreetoImmobilisedAmylaseEnzymeinItsCatalysisRate

ComparingFreetoImmobilisedAmylaseEnzymeinItsCatalysisRateMethod:Firstofall,theImmobilised enzymesneedtobemade.ThemethodusedtocreatetheseimmobilisedenzymeswouldbeMicroencapsulation. Thismeansthattheenzymeused,inthisexperimentbeingAmylase,isencapsulatedinsideSodiumalginateThe enzymewasbelievedtoactquickly,sotheenzymewouldhavetobeslightlydilutedinordertogetagoodrange ofresults.!0cm³ofAmylasewill...

EnzymeLab

ThepurposeofthisexperimentistodeterminehowthechangeofpH,enzymeconcentration,andtemperature affecttherateofenzymereactions.Inthisexperiment,threetestswereperformed.Thefirstrelatedtheeffectsof differentconcentrationsoftheenzymesucraseonaconstantamountofasubstrateThesecondexperimentwas usedtoinvestigatethechangesinenzymeproductivitybasedonthetemperatureatwhichthereactionwasallowed tooccurThethirdexperimentteststheeffectofhow

EnzymeAndTheEnzymeOfEnzymeEssay

Introduction:Anenzymeisamacromoleculethatworksasacatalystandspeedsupchemicalreactionsthrougha substratethatbindstotheactivesiteoftheenzyme.Whenthisprocessoccurs,theenzymelowerstheactivation energyneededThisallowsthereactantmoleculestoreachastatewheretheycancompleteareactioninmost temperatures.Theenzymeisnotconsumedwhenachemicalreactionistakingplace,however,it'sshapechanges inordertocreateaproperspaceforasubstratetobind

EffectsOfConcentrationOnTheRateOfReaction

ofsubstrateconcentrationconcentrationontherateofreaction"IntroductionEnzymescatalysebiologicalreactions ,increasingtherateofchemicalreactionsEnzymeshaveanaffectonthestructureofchemicaltransformation (stryeretal,2011)EnzymesubstancesbindattheactivecitewhichcontainsaminoacidchainsWithoutcatalysis reactionstheprocesswouldtakeplaceslowlytoproduceproducts,enzymesaremosteffectivecatalyst(Campbell andFarrell,2011)Acatalysedreactionis

CatecholOxidaseLabReport

MethodsEffectofpHonEnzymeActivityandBenzoquinoneAbsorptionDuringthisexperiment,theactivityof catecholoxidaseandtheabsorbanceofbenzoquinonewasmeasuredinfivesolutionswithdifferentpH concentration.Fivecuvettesprovidedbytheinstructorwereusedduringtheexperiment.Thetransmittanceonthe spectrophotometerwaszeroedonanemptychamberanditwassetatawavelengthof486nm.Becausetheshape ofanenzymechangeswithdifferentpHconcentrationandeachpHhas

Abstract:EnzymesarebiologicalcatalystsTheyarealsoproteinsandtherepropertiesaredeterminedbytheir structure.Thereactantisasubstrateandtheresultingfactoristheproduct..Enzymeactivityisinfluencedby manydifferentthingsincluding:substrates,products,presenceofcofactors,andinhibitorsTheeffectofinhibitors ismeasuredasapercentagecalledpercentinhibition.Duringthisexperimentweobservedthechangeinenzyme concentration,thechangeinsubstrateconcentration

HowEnzymeBreaksDownMolecules

Inordertosimulatehowanenzymebreaksdownmoleculesinacell,weusedtoothpicksandvariousmethodsto inhibitthisbreakdownprocess.Toothpickswereusedtorepresentthemoleculesinacell,andvariousfinger configurationswereusedtosimulatethebreakingofthemoleculesOurhypothesiswassupportedbecausethe dataindicatesthatwhenwevariedtheprocessthebreakingoftheenzymesvaried.Theseexperiments demonstratedthatwhenanenzymeprocesstobreakamoleculedownis

InvestigatingTheEffectOfTemperatureOnEnzymeActivity

temperaturesaffecttherateofenzymeactivity,withtheenzymecatalase.IntroductionThisexperimentisdesigned totesttheroleoftemperatureonenzymeactivityEnzymesare"moleculesthatfunctionasbiologicalcatalysts" (Reece2015).Theyactascatalystsbyspeedinguptherateofachemicalreactionby"loweringtheactivation energyneededforthereactiontobegin"(Reece2015).Theenzymebeingusedinthisexperimentiscatalase. Catalaseisanenzymethatisfoundinnearlyallliving

purposeofthisexperimentwastorecordcatalaseenzymeactivitywithdifferenttemperaturesandsubstrate concentrationsItwashypothesizedthat,untilallactivesiteswerebound,asthesubstrateconcentrationincreased, thereactionratewouldincrease.Thefirstexperimentconsistedoffivedifferentsubstrateconcentrations,0.8%, 04%,02%,01%,and0%H2O2Thesecondexperimentwascompletedusing08%substrateconcentrationand fourdifferenttemperaturesofenzymesrangingfromcold...

EffectOfEnzymeLabReport

EnzymesandFactorsThatAffectThemRewritetitleZuryabRanaOctober21,2017Abstract:Intheexperiment weusedTurnip,HydrogenPeroxide,DistilledWater,andGuaiacolasmysubstances.Onthefirstactivity,Effect ofEnzymeconcentrationofReactionRateforlowenzymeconcentration,wetestedthreeconcentrationsofthe turnipextract,andhydrogenperoxideFortheTurnipExtractIused05ml,10ml,and20mlForhydrogen peroxideweused0.1ml,0.2ml,and0.4ml.Weuseda...

EnzymeLabReport

ActivityofEnzymeAfomiaZewdieBIOL-1406-S4LLabJune30,2016AbstractThisexperimentwasperformed inordertodeterminehowtheconditionoftheenvironmentandthespecificitycanaffectenzymaticactivity.The activityofenzyme,howefficientlytheenzymefunctions,isaffectedbygeneralenvironmentalfactors,suchas temperatureandPH.Itcanalsoaffectedbychemicalsthatspecificallyinfluencethatenzyme.Inconditionoflow temperatureenvironment,theenzymesactivity...

WhyDoesTheConcentrationOfAnEnzymeFormedIncreaseWith Time

thisisbecauseenzymesarepowerfulcatalysts.Theycanacceleratethereactionsbyafactorofasmuchasa millionormoreInthisexperiment,withthelapseoftime,moresubstratebindstotheenzyme'sactivesiteof whichthesurfaceislinedwithaminoacidresiduewithsubstitutegroupsthatbindthesubstrateandcatalyseits chemicaltransformation.Enzymereducedtheactivationenergyofthisreaction.Thisenzyme-catalysedreaction takesadifferent'route'Theenzymeandsubstrateform

TheTypeOfBiomoleculesKnownAsEnzymes

Thisexperimentwasbasedonthetypeofbiomoleculesknownasenzymes.Thesemoleculesfoundinallliving things,andarecrucialfortheremovalanddecompositionofharmfulchemicals,thedigestionofmoleculesin metabolism,andthesynthesisofproteins,DNA,RNA,andmanyotherorganicmolecules.Severalkeyfeatures aboutenzymes(whicharesimilartocatalystsfoundinchemistry,onlyenzymesareorganicmoleculesthatare madeofproteinsorRNA)istheirreusability;anenzymewillnever...

HowTube3ChangedColorsIntoAnOrange-Brown

TherewasanimportantobservationmadeduringthisheatedexperimentwithTubes3and4Afterthemixture, Tube3changedcolorsintoanorange-brown,andTube4slightlychangedcolorsintoalightbrown.Figures5and 6demonstratetheresultsoftheeffectsa2degreesCelsiuscoolbathhadonsubstrateconcentrations.Therewere alsonotedcolorchangestotheseconcentrationsinTube3,whichturnedabrightorange,andTube4,whichalso turnedorange.Figure5.EffectofColdTemperature...

TheEffectOfPhOnTheRateOfCatecholaseCatalyzedReaction Essay

IntroductionWithoutenzymestheexistenceoflifeisquestionablesinceallmetabolicprocessesinthecellcannot occuratafasterrateenoughtosustainlife.Alltheessentialbiologicalreactionsinlivingthingsdependon enzymes'catalyticactivityEnzymesareusuallyproteins,thoughsomeRibonucleicAcid(RNA)moleculesactas enzymestoo,thatspeeduptherateofbiologicalreactionswithoutbeingconsumedbythereaction.(Reece,2016, p.83)Forinstance,thepresenceofenzymesintheconversion...

BiologyEnzymeExperiement

AnenzymeisabiologicalcatalystItspeedsupareactionbyloweringtheactivationenergyrequiredtostartthe reaction.Itspeedsupareaction,butremainsunchangedunlesscertainlimitingfactorsareintroduced.Itis composedofpolymersofaminoacidsAnenzymehasanoptimumpHandtemperatureWhenanenzymeisatits optimumconditions,therateofreactionisthefastest.Intheirglobularstructure,oneormorepolypeptidechains twistandfold,bringingtogetherasmallnumberof

ChemicalReactionsOfTheChemicalReaction

bysomethingcalledEnzymesEnzymesarebasicallycatalystthatareorganicwhicharemadeupofhundredsof aminoacidsandarepertainedasfairlylarge(BiologyDepartment,n.d,p.34).Similartoothercatalyst,enzymes havethecapabilityoffulfillingtheirfunctionwithoutallowingthemselvestobeconsumedaswellasdisfigured Enzymescanalsocarryontheirfunctionwithoutchangingthechemicalequilibriumbetweenreactantsand products(CooperGM,2000)Enzymesmainfunctionistohelp

Effectofdifferentsizebeadsontheactivityofimmobilisedcatalyse

ofdifferentsizebeadsontheactivityofimmobilisedcatalyseAim:Tofindouthowvaryingthesizeofbeads containingyeastvariestherateofactivityofcatalaseinafermentationreactionofhydrogenperoxidewith immobilisedenzymesAcatalystisasubstancethatspeedsuptherateofachemicalreaction,withoutitselfbeing usedupintheprocesshoweveritmaybeaffectedphysicallyi.e.degradationofthesurfaceofthecatalyst.

Catalystsworkbyreducing

HowPhAndPeroxidaseAffectsEachOtherAndTheEnzymeWas Made

TaraBlanchardBIOLabsec.09NickR.24October2015EnzymesAbstract:Enzymes,catalyticproteinsthatatas catalysiswhichmakestheprocessofchemicalreactionsmoreeasilyTherearetwomainfactorsthatactually affectsenzymesandtheirfunctionswhicharetemperatureandpH.Throughoutthisexperiment,thestudyhowpH andperoxidaseaffectseachotherandtheenzymewasmade.Therecordingsofhowtheenzymesrespondedwhen itwasexposedtofourdifferentpHlevelstocomeupwithan

InthislabweexperimentedwithpH,spectrophotometry,andenzymeslikecatecholaseWeused spectrophotometrytodetecthowenzymeactivitywouldchangeunderdifferentpH,temperature,enzyme concentration,andsubstrateconcentration.Enzymesareimportantforbiochemicalreactionsbecausetheyspeed uptheprocessandallowtheorganismtocontinuelivingTheenzymeusedintheexperimentiscatecholaseandit catalyzesareactionbetweencatecholandwater.Weanalyzedthesamplesinthespectrophotometry...

TheEffectofEnzymesonAppleJuiceProduction

TheEffectofEnzymesonAppleJuiceProductionBiologyPeriod6BackgroundInfoThepurposeofthis experimentwastodeterminewhichenzymeorthecombinationofthetwoenzymesmakesthemostapplejuice fromapplesauceWedidhisby,mixing10dropsofanenzyme(cellulose/pectinase/both)intoafewtablespoons ofapplesauceWethenlefttheresttonature,andwatchedtheliquidfunneloutfor10minutesLastly,we recordedourdata.Thecellwallisacomplicated...

EnzymeLab

IntroductionEnzymesareorganiccatalyststhatspeedupchemicalreactionswithinthebodyEnzymesarespecific foroneparticularreactionorgroupofrelatedreactions.Enzymesarelargeglobularproteinmoleculeswithoneor moreindentationsontheirsurfacecalledactivesites,anactivesiteisthepartoftheenzymethatbindstothe substrate,theactivesitehasaspecificshapethatwillbindtoaspecificsubstrate.Foranenzymetocatalysea reactionthesmallsubstratemoleculesmust...

ofthethree-partexperimentprovideadeeperknowledgeaboutthefactorsthatinfluencetherateofthereactionof theenzymeactivityandhowthefactorsinfluencethestructureorfunctionoftheenzymeAfterconductingthe experimentinPartA,theresultswerethesameasthehypothesisthatasthenumberofdisksincreasestheenzyme activityalso,proportionallyincreasesimultaneouslyshownclearlyinfigureachieved.Whenthreediskswere utilized,increasingtheenzymeconcentrationenabling

HowDoesTemperatureAffectsEnzymeActivity

:Enzymesaremadeupofproteinsandtheirfunctionismainlydeterminedbytheirstructure.Theprimary structureofanenzymeisdeterminedbythesequenceofaminoacidsThesecondarystructureisbasedonthe foldingoftheprimarystructure.Lastly,thetertiarystructureisbasedonthefoldingofthesecondarystructureand itsinteractionsofthesidechainsThepurposeofanenzymeistospeedupchemicalreactionsinacell Temperatureisafactorthataffectsenzymeactivitybecause...

CatalaseLabReport

IntroductionEnzymesarefoundinhumans,animalsandplants.Anenzymeisaproteinthatactsasacatalyst, whichbringsoutabiochemicalreactionBiochemicalreactionsareimportantinhumansbecausetheyhelpwith manyimportantfunctionssuchasdigestionandthebreakingdownofwasteinthebody.Catalaseisanenzyme foundinalmostalllivingorganismsthatareexposeddailytooxygen(suchasfruits,vegetablesandanimals)To determinehowCatalasereactswithhydrogenperoxide(a...

TheEffectofHydrogenPeroxideConcentrationonCatalaseActivity

Becauseofthis,humansalsoproducetheenzymecatalaseperoxidasesinordertoconvertsmallamountsofH2O2 intooxygenandwater.Itusesthefollowingchemicalformula:2H2O2→O2+〖2Hã€2OThisprocessis calleddecompositionastwohydrogenperoxidemoleculesarebrokendownintooxygenandtwowatermolecules Catalaseisanimportantenzymethatprotectscellsfromoxidativedamage,whichhydrogenperoxidecancause.It isanincrediblyefficientenzymewhereonecatalasemoleculecanconvert

LipaseLabReport

Theenzymelipase,ispresentinone'sbody,specificallyfoundinanindividual'spancreas,mouth,andstomach. Lipaseusesseparatefatstobreakdownanyconsumedfoods,sothattheycanproceedandbeabsorbedinthe intestinesThebodytypicallyproduceslipasenaturallybutinsomecases,peoplemayneedtouselipase supplementstoassistthemindigestingfood.Thetesttomeasurethelipaseenzymelevelsinyourbodyis sometimesknownasserumlipaseThistestmeasurestheamountoflipase

RealWorldConnectionLabReport

RealWorldConnections/DiscussionInbothexperiments,therewasoneenvironmentthattheenzymeworkedbest itThegraphsshowthatinthesaltexperiment4%saltconcentrationwastheoptimumenvironmentforthe enzyme,andpH6buffersolutionwastheoptimumpHenvironmentfortheenzyme.Intheotherenvironmentsthe enzymedidnotworkasfast,thereforenotcreatingasmuchproduct.Thehypothesiswasthattheenzymewould workthemostefficientlyinthemostneutralenvironmentThedata

Peroxidase

WhatistheoptimalpHforthefunctioningoftheenzymeperoxidase?Kranz,JBSC110L-H009:12October 2016Introduction:Enzymesareproteinsthatareusedtocatalyzeachemicalreactionbyreducingtheactivation energynecessaryforthereactiontotakeplace.Enzymesarehighlyspecifictoaparticularsubstrateandfunction bestatoptimalpHrangesandtemperatures(Bursal,2013)Peroxidaseisanenzymewhichcanbefoundinmany plantsandanimals,suchashorseradishandhumans.The...

YeastEnzymeLabReport

theclassconductedmultiplelabstotesttheenzymefunctionwithinyeasttoproduceCO2Eachgroupcompleted adifferentexperimenttotesttheeffectsofenzymefunctionwithinyeast.Thegroupsdidatotaloffourdifferent typesofexperiments:changingthetemperature,changingthepH,changingsubstrateconcentrationandchanging thesubstratesthemselvesTheseexperimentswouldallowthegroupstodetermineidealconditionsfortheenzyme functionwithinyeasttoproduceCO2.Results...

FactorsThatAffectTheRateOfAnEnzyme

occurtothereactionrateofanenzymeifthetemperature,pHlevel,concentrationofasubstrateandenzymewere changed.Inthisexperiment,theenzymecatecholase,commonlyfoundinplants,wastestedanditsproduct formation,benzoquinonewasmeasuredWhentheconcentrationofthereactantssuchastheenzymecatecholase, andthesubstratecatecholweretested,theresultsofproductformationincreasedgreatlybecausethehigher concentrationsallowedtheenzymestoincreasetheiractivityrates...

AbstractEnzymesarebiologicalcatalysts.Theyworkbyloweringtheactivationenergyneededtoinitiatea chemicalreactionEnzymesworkwithinanoptimaltemperatureandoptimalpHEnzymesarehighlyspecificfor asinglesubstrate.TheEnzymeisusuallymuchlargerinsizethanthesubstrateitbindsto.Insomecases,an enzymerequiressomethingcalledacofactortobeginthechemicalreaction.Therewerefourdifferentexperiments thatwereexecutedintheenzymelabExperiment71,the

EnzymeKineticsofBeta-Galactosidase

AbstractThisexperimentistostudyandmeasuretheenzymeactivityofβ-galactosidaseinthedifferent concentrationsofo-Nitrophenylgalactoside(ONPG)usingaspectrophotometerThespectrophotometerwasalso setat420nm,awavelengthwhichisbestforrecordingtheabsorbancevaluesfortheexperiment.Fromtheresults, 09mMONPGsolutionhasthehighestabsorbanceand01mMONPGsolutionhastheleastAlso,05mMONPG solutionhasthehighestrateofenzymeactivityanditisthemostefficient...

HowToIncreaseEnzymeConcentrationAffectsReactionSpeed

Increasingenzymeconcentrationincreasesreactionspeed.Thisisduetothemaximumvelocitylevelinwhichthe enzymeisabletobeactiveAstheenzymeconcentrationincreasesitcompensatesfortheamountofrestriction factorssuchastemperature,pHandsubstratelevels;allofwhichincreasestheireffectivenessduetoanimbalance insubstrate(Eed,2013)ThiswasshownusinganincreasedPyrosphosphataseconcentrationthroughoutthe experiments.Asshowninfigure1,theenzymehydrolysis...

InvestigateIfGarlicWouldKillBacteria

explainshowenzymesworkAsseeninFigure1theInducedFitModelshowshowtheactivesiteofanenzyme mustbecomplementaryinshapetothesubstratetoensurerecognitionandbindingoccurbetweenthetwo moleculesCatalaseisanenzymefoundintheliverthatundergoesrecognitionandbindingwithhydrogen peroxide(H202)tocreatewater(H2O)andoxygengas(O2).Theeffectoftemperatureonenzymeactivity TemperaturehasanegativeandpositiveeffectonenzymesAsthetemperature

HowHeatEffectsTheEnzymeActivity

Heateffectstheenzymeactivitybyspeedingupthereactionand/orcompletelydenaturestheenzyme.Whenthe yeastandhydrogenperoxidemixturewasputintothe80degreeC,theamountofO2mLevolvedconstantly stayedat0mlforthecomplete10minutescontrarytotheroomtemperaturewaterinwhichtheamountofO2 evolvedincreasedbyaboutanaverageof7mLforabout6minutesandthenincreasebyabout1-2mL.An enzymedenatureswithhightemperaturebecauseheatchangestheshapeof

TheReactionOfAnEnzymeEssay

Anenzymeisamacromoleculethatactsasacatalyst,achemicalagentthatspeedsupareactionwithoutbeing consumedbythereaction(Coleman2016)WithdifferentpHsandtemperaturestheenzyme'srateofreactioncan drasticallyalterForexample,peroxidase,atypeofenzymethatisfoundinvariousplanttissues,wasusedtolook intothereactionsofGuaiacol+H2O2->Tetraguaiacol+2H2Omixedintotesttubes.Followingtheextractofthe enzymetheprocessofstandardizationwastaking

TheEffectOfTemperatureOnTheEnzymePeroxidase

TheEffectofTemperatureontheEnzymePeroxidaseÂÂÂTheobjectiveofthisexperimentistoexplorethe effectoftemperatureontheenzymeperoxidaseTocomprehendtheeffectthattemperaturecanhaveonenzymes, specificallyperoxidase,onemustunderstandwhatenzymesareandwhattheirfunctionis.Enzymesareproteins thatarefoundincellsthatfunctionascatalyst(MsChang'sEnzymeNotes)Whatismeantbythisisthatenzymes increasethespeedofchemicalreactionswithoutchanging...

IntroductionEnzymesareproteinsinlivingorganismsthatspeedupchemicalreactionsandarealsovitalin makingsureourbodiescanfunction.Thesechemicalreactionsareknowascatalysts.However,enzymescannot createthesereactionsbythemselves.So,theyworkwithmoleculescalledsubstratestohelpthemout.Theenzyme andsubstratewouldusuallyfitperfectlytogether,unlesstheenzymelosesitsshapeTwomajorthingsthatcan impactthedisfigurationoftheenzyme'sshapearechanges...

EnzymeControlledReactionLabReport

ToinvestigatetheeffectoftemperatureonanenzymecontrolledreactionIntroductionandplanningForthe investigationofenzymes,Iamgoingtoconductanexperimenttoseehowtemperaturecanaffecttherateof reactionofenzymesbytestingitwithstarchTheenzymethatwearegoingtouseiscalledamylaseWearegoing totestthisenzymewithstarch.Bymixingamylaseandstarchsolutionstogetherunderdifferenttemperature conditions,wecanrecordtherateofreactionbytakinga...

PotatoCatalaseLab

HowDoesTheEnzymeConcentrationChangeTheConcentrationOf Trypsin

hydrogen,carbon,andoxygen.Proteinsareoftenreferredtoasbeing'thecentralcompoundforlife'.(Moulton)An enzymereferstoaproteinthatfoldsintocomplexshapes,whichallowsmallermoleculestofitinsideofthem,this isknownastheactivesite.Enzymesarebiologicalcatalyststhatspeedupchemicalreactionsinlivingorganisms. (Abpischools.org.uk)Trypsinisanenzymeimportanttothedigestionofproteinsinthebody.Theproteinis producedinthehumanbody,withinthepancreas

Temperature,pHandenzymeconcentrationhaveonEnzymeCatalaseTiffanySieversBiology110,Community CollegeofBaltimoreCountyAbstractThepurposeofthisexperimentwastotesttheeffectsthattemperature,pH, andsubstrateconcentrationhaveonanenzymecatalasereaction.Whataffectswilltemperature,substrate concentration,andpHlevelshaveonanenzymescatalasereaction?Temperature,pHlevelsandsubstrate concentrationwillhaveaneffectontheenzymescatalyzedreaction...

TheEffectOfTemperature,Ph,SpecificityAndCofactorsOfEnzyme Activity

specificityandcofactorsofenzymeactivityweretestedAnenzymeisanorganiccatalystthatspeedsupmetabolic reactionsbyloweringtheactivationenergyincells.Activationenergyistheminimumamountofenergyneeded forthechemicalreactiontooccurandcanbeaffectedbyalteringtheenzyme'senvironmentasshownduringthe experimentsWhentheenzymeisnotattheoptimaltemperatureorpH,thentheenzymemaybecomedenatured, inactiveandloseitsnormalshape.TheenzymeusedwasLactase,...

EffectsofpHonEnzymeActivity

AimToinvestigatetheeffectsofpHonenzymeactivityResearchquestionTowhatwillpHlevels1,7,12affect therateatwhichlivercatalasecatalyzesthedecompositionof10mlofhydrogenperoxide(H2O2)towater(H2O) andoxygen(O2)exemplifiedbythevolumeoffoamreachedin1minuteHypothesisSincetheoptimumpHof livercatalaseisapproximately7.IexpectthegreatestvolumeoffoamtobeachievedatpHof7becauseitisthe optimumpHoflivercatalase.Ialsoexpecttoseelower...

TheEffectOfCatalaseOnEnzymeActivityEssay

EffectsOnEnzymeActivityThisexperimentteststhereactionrateoftheenzymecatalaseEnzymesareproteins andtheyhelptospeedupchemicalreactions.Catalaseisanenzymethathelpstoeliminatetoxinssuchas hydrogenperoxidewithinanorganism(NuffieldFoundation)Whenenzymaticreactionsoccurthereactantis knownasasubstrate.Withinanorganismenzymesareinarathercontrolledenvironmentwheretemperatureand pHlevelstayinatightrange(DominicCorsini)Thehypothesis

AnInvestigationontheRateofReactionoftheEnzymeCatalaseonthe SubstrateHydrogenPeroxide.

AnInvestigationontherateofreactionoftheenzymeCatalaseonthesubstrateHydrogenperoxidePlanAim:To investigatetherateoftheeffectofCatalaseonhydrogenperoxide.IntroductionThisinvestigationwillbecarried outtoinvestigatetherateofreactionoftheenzymecatalaseonthesubstratehydrogenperoxideEnzymesare biologicalcatalysts,whichspeeduptherateofreactionwithoutbeingusedupduringthereaction,whichtake placeinlivingorganismsTheydothisby

BiologicalBranchesOfTheComplexStudyOfLife

complexstudyoflife.Amylase,anenzymethatcatabolizesstarchpolymers,isoneofthemostimportantenzymes neededfortheproductionofcertainfoods,suchassyrups,anddifferentprocessessuchasfermentation.Like everything'sbiologicalnature,thesecertainenzymesareaffectedbydifferentfactorsrangingfrompHlevelsto temperature.Findingoutthetemperatureatwhichtheseenzymesreachtheiroptimalcondition(conditionsat whichtheseenzymesworkthebest),isoneofthemost

InvestigatingWhichLactaseEnzymesWereMostEffectiveAt

Inthisexperiment,theresultsidentifythedifferenttemperaturesthatthelactaseenzymesweremosteffectiveand ineffectiveatAt0°,1%ofglucosewaspresentinthemilkmeaningthatthelacteezeenzymeswereeffectiveat thetemperaturebutthereactiontimeoftheenzymeswereslower.At40°,themilkcontainedthemaximum amountofglucoseat2%ormore.Thisindicatesthatatthistemperature,thelacteezeenzymesworkthemost effectiveandtheyhavethemostamountofcollisionswhich

TheEffectOfPh,EnzymeConcentrationAndTemperatureOnThe CatalyticRateOfEnzymesEssay

TheEnzymePropertiesLabwasperformedtoanalyzetheeffectsofpH,enzymeconcentration,andtemperature onthecatalyticrateofenzymes.Manyfunctionswithinthehumanbodyrequire/involveenzymes.Itwasfound thatenzymeshaveanoptimalpHlevel,catalyticrateincreasesastheconcentrationoftheenzymeincreases,and anoptimaltemperature.TheenzymethatwasusedthroughouttheexperimentwasALP(alkalinephosphatase enzyme)ALPfunctionsbestataneutralpHlevel,asithadthe

TheEffectOfLowPhOnEnzymeActivity

TheEffectoflowpHonEnzymeActivityNameInstitutionAffiliationOutlineEnzyme:AmylaseSource:Barley Substrates:StarchfrompotatoesIodineMethod:Time-basedexperimentontheenzyme'sreactiontoconverting Starchintosugarsbytestingtheamountoftimeinwhicha2cm3ofstarchsolutionwouldbeconvertedtosugars ControlGroup:•AmountofIodine•AmountofStarchSampleSize:1.50gIntroductionEnzymesare typicallyproteinsandactascatalystsforvarious

ScientificMethodology&EnzymeActivity4/28/15Thepurposeofthisexperiementistodeterminetheeffectof temperatureontherateofenzymereaction,specificallytestingthereactionratesat0,23,37,and55degrees Celcius.Introduction:1.AccordingtothebookCampbellBiology,anenzymeisabiologicalcatalystthatreduces theactivationenergyinareaction,allowingthechemicalreactiontotakeplaceatafasterrateAsubstratebindsto theactivesiteofanenzyme,allowingachemical...

InvestigatingTheEffectOfPhOnTheReactionOfAPoultry(Enzyme

)ReactionEssay

theoutcomeofapoultry(enzyme)reactionGoalObserveandrecordwhathappenswhenenzymes(chicken)are addedtodifferentamountsofacid(hydrogenperoxide)mixedwithwater.Theactivityleveloftheseenzymeswill differandbedependentuponseveralfactors,suchasconsistency,sizeandshapeofthemoleculeSpecificfactor willbetheconcentrationenzymeandsubstrate.Iwillconductacontrolledexperimenttodeterminetheinfluence ofpHontheenzymecatalaseandthereleaseof...

CatalaseEnzymeLab

Introduction:Thecatalaseenzymeisasubstanceinsidealllivingthingsthatspeedsupchemicalreactionsand activities.Enzymesareproteinsthatlowertheactivationenergyofchemicalreactionsandworkonsubstrates, whicharetheproductbeingchangedEnzymesareveryspecificandcertainenzymesonlyreactwithcertain substrates,theactivesiteofanenzymeisthepartoftheenzymethatbindstothesubstrate.Theactivesiteandthe substratemusthavethesameshapeinorderforthis

EnzymesEssay

EnzymesareanimportantpartofallmetabolicreactionsinthebodyTheyarecatalyticproteins,abletoincrease therateofareaction,withoutbeingconsumedintheprocessofdoingso(Campbell96).Thisallowstheenzymeto beusedagaininanotherreactionEnzymesspeedupreactionsbyloweringtheactivationenergy,theenergy neededtobreakthechemicalbondsbetweenreactantsallowingthemtocombinewithothersubstancesandform products(Campbell100)Inthisexperimentthe