Novocastra HER-2 (c-erbB-2 Oncoprotein) Antibodies Clone CB11 1 mL, 0.1 mL lyophilized HER-2 (internal domain) NCL-CB11 F P C 1 mL liquid HER-2 (internal domain) NCL-L-CB11 F P C 7 mL ready-to-use HER-2 (internal domain) RTU-CB11 F P 60 Tests Leica Bond Oracle HER2 IHC System TA9145 P Clone 5A2 1 mL lyophilized HER-2 (internal domain) NCL-c-erbB-2-316 F P Clone 10A7 1 mL, 0.1 mL lyophilized HER-2 (external domain) NCL-CBE-356 P W 1 mL liquid HER-2 (external domain) NCL-L-CBE-356 P W 7 mL ready-to-use HER-2 (external domain) RTU-CBE-356 P Clone CBE1 1 mL lyophilized HER-2 (external domain) NCL-CBE1 P (HIER) Antigen Background The c-erbB-2 oncoprotein is closely-related in structure to the epidermal growth factor receptor and is a member of a large family of cell surface growth factor receptors. c-erbB-2 oncoprotein is reported to be detectable in a proportion of breast and other adenocarcinomas as well as transitional cell carcinomas. c-erbB-2 oncoprotein is present in a wide variety of cell types in a range of normal human fetal and adult tissues, including breast, stomach and ovary.
Product Specific Information Clones 5A2 and CB11 detect the internal domain of the c-erbB-2 oncoprotein. Clones 10A7 and CBE1 detect the external domain of the c-erbB-2 oncoprotein. NCL-CB11 is effective with no pretreatment on fixed, paraffinembedded tissue but the use of the heat induced epitope retrieval (HIER) technique may enhance staining in some cases. To obtain optimal staining on frozen tissue, Carnoy's fixative is recommended. For more information on the Oracle HER2 Bond IHC System see page 11.
Human breast carcinoma: immunohistochemical staining for c-erbB-2 oncoprotein using NCL-CBE-356. Note intense membrane staining of tumor cells. Paraffin section.
Novocastra Herpes simplex virus Antibodies Clone 20.7.1 1 mL lyophilized Herpes simplex virus (type 1) NCL-HSV-1 P (Enzyme) I 12.3.4, Clone 1.1.1 1 mL lyophilized Herpes simplex virus (type 2) NCL-HSV-2 I Infection with Herpes simplex virus (HSV) is extremely common and pathogenesis can vary depending on a variety of factors. These include age, immune status of the individual, the antigenic type of infecting virus (HSV type 1 or 2) and the site of infection. Primary infections with HSV are generally asymptomatic but they tend to be more severe than recurrent productive disease.
Product Specific Information NCL-HSV-1 is HSV type 1 specific and does not cross-react with tissue culture grown HSV type 2 strains. NCL-HSV-2 is HSV type 2 specific and does not cross-react with tissue culture grown strains of HSV type 1.
Leica Bond Oracle HER2 IHC System, TA9145. Herpes simplex virus type 1 (HSV1) infected HEp-2 cells: indirect immunofluorescence for HSV1 using NCL-HSV-1. Note intense staining of HSV1 infected HEp-2 cells only. Acetone-fixed cells.
F Frozen
I Immunouorescence
E Electron microscopy
P ParafďŹ n
C Flow cytometry
O Other applications
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W Western blotting