Impedance-Based Wound Healing Assays using ECIS With ECIS’s impedance measurements, functional wound healing assays can be performed with unrivalled convenience. After wounding cells on an electrode with an electric pulse, ECIS’s labelfree impedance measurement technology allows the user to measure the wound healing process in real time. ECIS standard 96 well screening plates are designed for high-throughput applications, such as screening of migration inhibitors.
Input:
Output:
• Cells (50,000 per well)
• Time taken for wound healing per compound
• Medium, compounds (300 µl per well)
Wounding
10
15
20
Time [h]
14 12 10
......
Compound 96
5
Compound 95
0
16
Compound 7
1000
18
Compound 6
2000
20
Compound 5
Inhibitor
Compound 4
Normal
Compound 3
Enhancer
3000
Compound 2
4000
22
Control
5000
Compound 1
Impedance (Ohm)
Control
6000
Duration Wound Healing [h]
24
7000
0
Live Cell Screening Imaging
The general effects of enhancers and inhibitors on wound healing are provided below, along with typical data from migration inhibitors screenings.
Typical Migration Data Obtained Using ECIS Technology The emptied electrode is re-covered with cells after 14 hours. The graph shows that different inhibitors slow down the wound healing process. The ECIS principle is explained in detail starting from page 138.
Screening in ibidi’s Multi-Well Plates The ibidi technology used with µ-Plates 24 well, µ-Plates 96 well, and µ-Plates 384 well (see page 89) provides all the important features required for enhanced microscopy: a coverslip like bottom of standard No.1.5 thickness with extremely low autofluorescence, and the unique ibiTreat tissue culture treatment that makes the µ-Plates biocompatible, as proven in ibidi’s µ-Slides and µ-Dishes. Another outstanding feature of ibidi’s µ-Plates is their excellent stability with all common solvents used in cell-based assays.
ibidi FREE SAMPLES Order a free sample at www.ibidi.com to test ibidi’s Multi-well Plates with your experiments.
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