Collagen Type I N
A high quality, rat tail collagen solution used for creating 3D collagen gels (e.g., in chemotaxis assays)
Live Cell Imaging
EW
• Provides in vivo–like ECM (extracellular matrix) structures • High concentrated collagen solution – usable for a variety of gel concentrations • Fast polymerizing for optimal cell distribution in gel • Non-pepsinized, native collagen for a close-to-nature situation
Applications • Chemotaxis assays in firm 3D collagen gels • Leukocyte and tumor cell migration • 3D cell microscopy • Culturing cells on thin or thick collagen layers • Coating of cell cultureware to promote cell attachment • Differentiation studies – 2D versus 3D High resolution microscopy during chemotaxis and cell polarization, using the HT-1080 LifeAct cell line (see page 34)
• Highly suited for live cell imaging and fluorescence microscopy
Technical Features: • High-quality purified protein from rat tendons • Ideal for use in combination with µ-Slide Chemotaxis 3D (see p. 109) • Ready to use • Highly standardized manufacturing process • Stringent quality standards Cell spheroid (or micro tumor) on top of, or embedded in, a 3D matrix for long-term cultivation. The upper well provides nutrients by means of diffusion. Specifications : Rat tail tendon
Appearance
Optically clear viscous liquid
Extraction
Acid, non-pepsinized
Purity
> 90 % by SDS PAGE
Sterility
Sterile, for cell culture
Contaminants
Negative for DNA, bacteria, fungi, and mycoplasma
Growth factors None
52
Supplied in 17.5 mM acetic acid (0.1%)
pH
pH 3.8
Functional control
3D gelling and 2D coating test in cell culture
Storage
• Storage at -20°C for well-defined quality and reproducibility
ibidi APPLICATION NOTE
Source
Formulation
• Simple and easy protocol and handling
-20°C
The Application Note Collagen I Gel in 3D Cell Culture (AN 26) provides detailed protocols on how to prepare collagen I gels with different cell culture media and different types of collagen.
Ordering Information: Cat. No.
Description
50201
Collagen Type I, rat tail, non-pepsinized, 5 mg / ml, 1 x 5 ml
Pcs. / Box 1
50202
Collagen Type I, rat tail, non-pepsinized, 5 mg / ml, 4 x 5 ml
1
50203
Collagen Type I, rat tail, non-pepsinized, 5 mg / ml, 1 x 100 ml
1
50204
Collagen Type I, rat tail, non-pepsinized, 10 mg / ml, 1 x 5 ml
1
50205
Collagen Type I, rat tail, non-pepsinized, 10 mg / ml, 4 x 5 ml
1
50206
Collagen Type I, rat tail, non-pepsinized, 10 mg / ml, 1 x 100 ml
1