Gene Therapy & Molecular Biology Volume 2 Issue A

Figure 7. The effect of c-myc overexpression on IL-1-inducible growth inhibition and hypophosphorylation of RB. (A) C6/myc.L1 or vector.L1 cultures were exposed to IL-1 or vehicle for 24, 48, and 72 h, and then subjected to [3 H]thymidine incorporation studies. Each value point is a mean of 12 observations from 3 different experiments. Error bars indicate Âąstandard deviations. (B) Spheroids produced from C6/myc.L1 or C6/vector.L1 cultures were exposed to IL-1 or vehicle, and 3-dimensional growth was determined at the various time points indicated. Each value point is a mean of 24 observations from 3 separate experiments. (C) C6/myc.L1 or C6/ vector.L1 transfected cells were unexposed (-) or exposed (+) to IL-1 for 48 hours, and then whole-cell protein extracts were prepared from the cells and subjected to Western blot analysis using the anti-RB antibody. The slow-migrating differentially phosphorylated forms of RB (pRB) and the fast-migrating hypophosphorylated form of RB (RB) are indicated.