Results: A total of 560 patients have been recruited and results were available for analysis in 533 patients up to Jan 2019. The demographic characteristics and antibiotic resistance rates were similar across the two treatment groups. The eradication rate in the second line treatment were 88.3% (235/266) and 88.4% (236/267) in the levofloxacin sequential therapy and bismuth quadruple therapy groups, respectively (p=1.000) in the ITT analysis. The eradication rates were 89.7% (235/262) and 92.9% (236/254) in the levofloxacin sequential therapy and bismuth quadruple therapy according to PP analyses, respectively (p=0.195). The efficacy of levofloxacin sequential therapy, but not bismuth quadruple therapy, appeared to be affected by levofloxacin resistance. The frequency of any adverse effects was higher in patients treated with bismuth quadruple therapy than levofloxacin sequential therapy (76.4% vs. 44.1%, p＜0.001). Conclusions: Levofloxacin sequential therapy and bismuth quadruple therapy are similarly effective in the second-line treatment for H. pylori infection (Trial registration number: NCT NCT03148366). 09:10-09:20 PROBIOTICS-CONTAINING YOGURT INGESTION AND H. PYLORI
ERADICATION CAN RESTORE FECAL FAECALIBACTERIUM PRAUSNITZII DYSBIOSIS IN H. PYLORI-INFECTED CHILDREN
Yao-Jong Yang1,2, Peng-Chieh Chen2, Fu-Ping Lai1, Bor-Shyang Sheu2,3 Departments of Pediatrics, National Cheng Kung University Hospital, Tainan, Taiwan1 Institute of Clinical Medicine, College of Medicine, National Cheng Kung University, Tainan, Taiwan2 Departments of Internal Medicine, National Cheng Kung University Hospital, Tainan, Taiwan3 Background: H. pylori infection can alter the diversity and richness of gut microbiota, and probiotics ingestion can improve H. pylori-induced gastric and systemic inflammation in vivo and in vitro. Aims: To investigate the compositional differences in fecal microbiota between children with and without H. pylori infection, and to test whether probioticscontaining yogurt and H. pylori eradication can improve dysbiosis in children. Methods: Ten H. pylori-infected children and 10 age- and sex-matched controls were enrolled to ingest probiotics-containing yogurt for 4 weeks. Ten-day triple therapy plus yogurt was given to the H. pylori-infected children on the 4th week. Fecal samples were collected at enrollment, after yogurt ingestion, and 4 weeks after successful H. pylori eradication. Fecal secretory immunoglobulin A and cytokines were measured by ELISA. The composition of fecal microbiota was checked through metagenomic sequencing of the V4 region of the 16S rRNA gene. The OTUs demonstrating major changes in relative abundance were identified by LEfSe and Metastats.
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