Progenitor Cell Analysis Core Ed Jachimowicz, MS jachie@mmc.org Pradeep Sathyanarayana, PhD sathyp@mmc.org This core provides instrumentation and expertise for cell analysis and isolation. Cell isolation (sterile) is via fluorescent-activated and/or magnetic technologies (FACS, MACS). The FACSAria-2 (BD) is a fixed optics alignment benchtop high-speed sorter. Digital electronics and variable flow pressures allow sorting with 8+ parameters/markers possible at up to 1,000 cells per second. The autoMACS (Miltenyi Biotec) utilizes immuno-tagging of cells with non-toxic iron oxide particles. Enrichments or purifications of cells of interest can be via either positive, or negative selection. MACS is scalable (and can be used a prior to FACS). Analyses of cell properties and parameters are supported by a BD FACSCalibur flow cytometer; Turner Biosystems fluorimeter & luminometer; and STEMVision system for imaging and analyzing cells cultured in 6-well plate formats. A tissue culture suite is also available, together with basic support for stem and progenitor cell culture. Services available include: Flow Cytometry Cell isolation / sorting (FACS, MACS) Training in flow cytometry (and analysis) Fluorimetric & luminometer assays (96-well format) Tissue culture Supported in part by COBRE P30GM103465
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