C3: Collaborating to Conquer Cancer Fall 2012

Page 9

DEC DINGCANCER HOW GOOD DNA GOES BAD

A

cell builds new DNA like zipping closed

well to subsequent tines, and the difficulty attaching the next

a winter coat, adding new zipper tines as the

building blocks sounds an alarm that signals the DNA polymerase

new DNA matches itself to the pattern DNA. But

enzymes that do the actual stacking to quickly fix the problem.

sometimes these two strands don’t stay perfectly zipped

And then even after DNA replication is finished, the new strand

together; sometimes they slip or wobble.

is compared to the old strand and any mismatched bulges or other

When the new strand slips, a little kink in the zipper can

abnormalities are pruned and repaired. Any errors that remain after

result in an extra nucleotide “tine.” When the pattern strand slips,

stacking and checking are coded into the DNA as mutations, but even

the kink can result in a missing nucleotide.

then cells with abnormal DNA aren’t likely to survive to replicate.

A wobble briefly makes a nucleotide “tine” on the pattern strand

Cells have to pass frequent tests of normalcy—like a Tour de France

able to grab a nucleotide tine other than its proper pairing—the

rider taking a drug test at the end of every stage—and cells that rush

wobble can insert the wrong nucleotide in the new strand. Most of

through their lifecycle like a doping rider aren’t allowed to continue.

these wobbles don’t last long—a mismatched tine doesn’t attach

Doping cells are pruned by apoptosis—programmed cell suicide.

NEW STRAND SLIP

PATTERN STRAND

PATTERN STRAND SLIP

NEW STRAND

PATTERN STRAND

NEW STRAND

MISMATCHED NUCLEOTIDE

EXTRA NUCLEOTIDE

MISSING NUCLEOTIDE

9 C3: WINTER 2012


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