XXI Biochemical Engineering National Congress X Biochemical Engineering International Congress XVI Biomedicine and Molecular Biotechnology Scientific Meetings Proceedings
Abstract
Antiulcer activity of the extract of phycobiliproteins of Spirulina maxima on gastric ulcers induced by ethanol in rats a
Oscar Guzman Gomez, bRosa Virginia Garcia Rodriguez, cLucia Quevedo Corona, Ricardo aPerez-Pasten Borja, dEmmanuel Rios Castro, aGerman Alberto Chamorro Ceballos.
aDepartamento
de Farmacia, Escuela Nacional de Ciencias Biologicas, Instituto Politecnico Nacional, Ciudad de Mexico, Mexico. bUnidad de Servicios de Apoyo en Resolucion Analftica, Universidad Veracruzana, Xalapa, Veracruz, Mexico. cDepartamento de Fisiologia, Escuela Nacional de Ciencias Biologicas, Instituto Politecnico Nacional, Ciudad de Mexico, Mexico. d Unidad de Genomica, Proteomica y Metabolomica, LaNSE, Cinvestav-IPN, Ciudad de Mexico, Mexico.
Introduction: Gastric ulcer is among the most common diseases affecting people in the world. Traditional medications can lead to adverse effects, therefore, alternative strategies are needed to prevent the development of gastric mucosal damage. Phycobiliproteins are billiproteins which exerts antioxidant and anti-inflammatory effects in various in vivo and in vitro experimental models. Objective: The aim of this study was to assess the possible gastroprotective activity of phycobiliproteins of Spirulina maxima (PhySm) against ethanol-induced gastric ulcer in rats through evaluation of ulcer index inhibition, antioxidant enzyme activities, lipid peroxidation and as well as histopathological damage. Methodology: Antiulcer activity of PhySm was evaluated by ethanol-in duced gastric ulcer model. PhySm was administered to rats intragastrically and it was applied during eight days to rats. The doses of PhySm administered daily to each group of rats was 100, 200, and 400 mg/kg, respectively, and 40 mg/kg for omeprazole. One hour after the last treatment, 80% ethanol solution (1 ml/rat) was administered. Results: PhySm (100, 200 and 400 mg/kg body weight) showed significantly increased in the ulcer index inhibition value in comparison with ulcer group (P<0.05), the better results were achieved with the higher dose tested. Histological studies supported the observed gastroprotective activity of PhySm. M oreover, the PhySm prevented alcohol-induced decrease in stomach antioxidant enzyme activities such as superoxide dismutase, catalase, and glutathione peroxidase with respect to those treated with ethanol alone. Furthermore, the administration of PhySm reversed the increase in lipid peroxidation induced by ethanol. Conclusion: Our results suggest that PhySm pretreatment could partly protect against ethanol-induced gastric damage in rats and these gastroprotective effects are mediated at least partially through an antioxidant mechanism. Keywords: Antiulcerogenic, Spirulima maxima, Phycobiliproteins, Ethanol.
Area Biomedicine and Health 21-23 March, 2018, Mérida, Yucatán, México
JBBR ©2018 Colegio Mexicano de Ingenieros Bioquímicos
168