FOGALE nanotech
Driving your bioreactor toward the future
CELL CULTURE & FERMENTATION
Driving your bioreactor toward the future
viable cell density for yeast, bacterial, fungal, animal and plant cells. It is suitable for the measurement of both very low and very high cell concentrations in complex media. With additional software programs, it can be transformed into a full Biomass Analyzers.
Access incredible benefits
with the FOGALE Biomass System
Optimize yield and shorten process development Increase control of your culture during scale-up Improve the level and quality of and traceability in production Online Biomass monitoring Measures in real time and records all growth Monitors cell density in the culture media Greatly decreases contamination risks during sampling Reduces the need for intensive lab analysis & eliminates human error
Biomass control has never been so easy ! An ideal instrument for closed loop control in batch, fed-batch and perfusion Keeping your cell density constant or optimizing your feed rate and timing is now very convenient
and all the advantages you want
available in a single model...
Stable and sensitive
in all culture conditions
Not limited
by media conductivity
Modular design
from 1 to 4 probes on the same control unit
Compact design
probes and pre-amplifiers fit all vessel sizes
User friendly
quick installation and easy handling
Frequency scanning and advanced softwares system upgradable at any time
...ALL for the best price For more detailed information: infobiotech@fogale.com / Tel. + 33 466 620 555, or visit our website at www.fogalebiotech.com
Benefits for
cell culture
From process development to cGMP environments, the Biomass System is the choice of many cell culture engineers to optimize their bioreactor operation strategy.
Outstanding performance: - at lab level to understand the process and/or optimize the control strategy - for large-scale manufacturing, including the identification of process deviation, of optimal harvest time and/or PAT application - in animal cell production, whether in suspension or attached to microcarriers - with all of the following cell lines: CHO, PER.C6, Vero, BHK, Hybbridoma, HEK 293, NS0, Hela, Sf9, Sp2/0
Applications Sf9 growth and infection process by a baculovirus (BEVS) Infection time
5.0 4.5
permittivity Biovolume
3.5 3.0
8.0 Linear Correlation Permittivity - Biovolume
2.5
Permittivity (pF/cm)
optimizes: - Time of infection - Successful baculovirus infection - Harvest time
Permittivity (pF/cm)
4.0
10.0
2.0 1.5 1.0
6.0
5.0 4.0
4.0
3.0 2.0 1.0
2.0
0.0 0
1
2
3
4
5
6
7
8
9
10
11
3
0.5
Biovolume (Âľm /ml)
0.0
0.0 0
20
40
60
80
CHO fed-batch cultivation
100 time (h)
120
140
160
Biovolume and viable cell count 14.0
accurate online viable cell count during industrial scale production
Biomass System
12.0
0.025
10.0
0.020
Advanced Cell Analyzer
8.0
0.015 6.0 0.010
4.0 FOGALE online viable cell count
Viable cell count (microscopy - trypan blue) 0.005 FOGALE Permittivity (Biovolume)
2.0
Offline Biovolume (microscopy)
0.0
0.000 0
2
4
6
8
10
12
14
Time (days)
For more detailed information: infobiotech@fogale.com / Tel. + 33 466 620 555, or visit our website at www.fogalebiotech.com
Biovolume
Permittivity (pF/cm) Viable cell count (E6 cell/ml)
and can be used to automatically trigger the start of feeding or to control a proportional feed, ensuring both proper timing and dosage.
0.030
Measurement
principle
properties of living cells; this application has the advantage of measuring only viable cell density. Unlike optical techniques, this system is insensitive to gas bubbles, microcarriers, cell debris or other particles in suspension. - Using FOGALE’s expertise in capacitive measurements and applying a unique and patented method, the Biomass System measures the viable biomass concentration (Biovolume), independent of cell size variations. - By scanning the entire frequency range and with precise measurements of permittivities, the β-dispersion spectra frequency (fc), the maximal permittivity difference (Δεmax), and the coefficient α the morphological and physiological state of cells. - FOGALE’s dielectric spectroscopy experience with animal cell lines brings the technique to a new level of achievement in measurement. Using a powerful patented method, the optional Advanced Cell Analyzer software Δεmax and the cell size distribution is correlated to the α parameters. Cell suspension -dispersion
Under the influence of an electric field, cells with intact plasma membranes (viable cells) are polarized and behave as tiny capacitors
PERMITTIVITY (pF/cm)
Low frequency plateau
max
FOGALE
= Biovolume
fc (critical frequency)
High frequency plateau
Sensors Ideal for scale-up in a cGMP environment
0.01
Probes are made of materials that conform to FDA standards (316L stainless steel, PEEK, platinum, EPDM). 12 and 25 mm diameter sensors are available to fit all port sizes, in a choice of 120, 220 and 320 mm in length to fit all vessels. All sensors are sterilizable in place (SIP) or in an autoclave.
0.1 FREQUENCY (Mhz)
1
FOGALE frequencies to measure viable cell density or Biovolume
10
100
Driving your bioreactor toward the future
Softwares X system: an intuitive software program for plotting and recording the biomass and conductivity. It is designed for quick installation and rapid probe configuration by the user.
Biomass +: a software package to customize your Biomass System as an online, real time comprehensive cell analyzer.
Biomass Spectrometer: (optional)
Industrial IP 65 enclosure for industrial environments
tracks the physiology of your cells. It describes in detail the process dynamics and can be used to control the culture, driving it through the optimal operating range. In process development, it has proven to be a valuable technology that enables process understanding(including an alert to process deviations and identification of optimal harvesting time), which in turn leads to applications in large scale manufacturing. cell-specific spectral fingerprint is a powerful tool for culture traceability and PAT application.
Advanced Cell Analyser: (optional) number of viable cells and cell size, and determines cell size distribution in real time. In addition, it calculates online the cells’ doubling time. Now you can compare online and offline data with consistency! VCD reference method, at the same time reducing the costs and eliminating the risks of manual sampling and counting.
Technical specifications BiomassSystem Concentration range
0 -109 cell/ml for animal cells 0 - 200 g/l dry weight for yeast and bacteria
Resolution Conductivity range
0.01 - 106 cell/ml for animal cell 0.02 g/l dry weight for yeast 0.5 - 100 mS/cm
Numeric outputs / serial port
RS 232 C/Mod BUS protocol data transfer
Analog outputs
4 - 20 mA or 0-10 V for Biomass / Conductivity
Number of channel
monitor up to 4 probes simultaneously It is possible to start with one probe, and to add more later
Dimensions
Standard for rack mounting: 450 x 270 x 150 mm (19", 3U) Stainless IP 65 enclosure: 350 x 170 x 460 mm
Benefits for
fermentation
gas bubbles, cell debris and other particles in suspension.
- Precise and linear, up to extremely high cell densities of bacteria, filamentous fungi or flocculating yeasts - Convenient for controlling a fed-batch process, continuous fermentation, or programming an induction time based on a defined biomass target - Stable and accurate in the highly conductive media occuring in fermentation
Applications Filamentous fungi fermentation monitoring Filamentous fungi fermentation monitoring Absidia (pellets)
biomass (Absidia corymbifera) in a highly aerated media.
Pichia pastoris growth and fed-batch control T1
20,0
T2
18,0
70
Production on methanol
Growth on glycerol
60
12,0
40 Linear Correlation Permittivity - OD
10,0
4,0 2,0
Harvest Time
6,0
(OD 600 nm)
30
8,0
Per mi t t i v i t y ( p F / c m)
20
10
0,0
0 0
20
40
60
T ime (h)
80
100
Optical density OD (600nm)
50
14,0
Induction Time
- Cell growth on glycerol - Induction time (T1) - Fed-batch on methanol - Harvest time (T2)
Permittivity (pF/cm)
16,0
FOGALE Nanotech, known as the worldwide reference for viable biomass monitoring based on capacitance, provides specific standard & integrated solutions.
Exper
tise d an
ormance Perf Wherever your business is located, our worldwide support provides the service you deserve. Our team of experts will help you set up new systems and will provide full-scope customer service. Our sensors and systems, our experience and knowhow, our professionalism and commitment are the guarantee of your entire satisfaction.
Distributor
Europe/Asia: FOGALE nanotech, France +33 466 620 555 - infobiotech@fogale.com
For more information about our products, please call us at +33 (0) 466 620 555 or visit our website www.fogalebiotech.com
USA: FOGALE nanotech Inc, Los Angeles, CA / (310) 348 2974 - usabiotech@fogale.com