ADCC NF-kB-Luc Reporter Effector Cell Line, Human Jurkat T Lymphocytes
Cat. No.: AD-P001-C
This product is for research use only and is not intended for diagnostic use.
Overview
Description
Human NF-kB-Luc Reporter T Lymphocytes have the Lucia luciferase gene (a secreted luciferase reporter gene), driven by the IFN-β minimal promoter, which is fused with a total of 5 copies of NF-κB transcription Response element and 3 copies of c-Rel binding site.
Human NF-kB-Luc Reporter T Lymphocytes also express a secreted embryonic alkaline phosphatase (SEAP) reporter gene, which works together with five IFN-stimulated response elements under the control of the ISG54 minimal promoter.
As a result, JHuman NF-kB-Luc Reporter T Lymphocytes can simultaneously study the NF-κB pathway by monitoring the activity of Lucia luciferase and the IRF pathway (by evaluating the activity of SEAP).
Features
•Stable expression of Fc receptor CD16A on cell surface
•Stable expression of Luc luciferase reporter gene under the control of ISG54 minimal promoter fused with six NFAT response elements
Applications ADCC reporter effector cells
Specification
Size 3-7 x 10e6 cells
Contents Two kit formats are provided: Complete, everything you need to get started Core, used with customer-defined antibodies and target cells
Cell Background Jurkat T Lymphocytes
Sample Works with both adherent and suspension cells.
Cell Purity >95%
Cell Viability >90%
Sterility Testing
Creative Biolabs provides sterility testing in accordance with USP and EP regulations. All of our sterility testing is performed in an isolator or clean room environments. The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.
Identity Testing
Identity testing is required for newly established cell lines. Isoenzyme analysis is used to confirm the identity of the species of a cell line. Alternative methods for identity testing include DNA fingerprinting, STR analysis and karyology.
Virological Safety Testing
A broad range of viruses is susceptible to affecting human cell lines. We can provide in vivo/vitro virus saftey assays by utilizing various animal systems. These viruses include: adventitious viruses, bovine viruses, human and simian viruses, porcine viruses, retrovirus and rodent viruses.
Genetic Stability TestingWe perform cell genetic stability studies under ICH guidelines. We can provide guidance on the appropriate testing program upon your requirements.
Mycoplasma Testing
Shipping
Storage
Warnings
Research Use Only
Images
Guaranteed mycoplasma-free
Dry ice
-80°C for short-term storage, liquid nitrogen for long-term storage
Avoid multiple freeze/thaw cycles
Our recombinant cell are for research use only, not for diagnostic or therapeutic use.
