alleles
Suspect D
Suspect C
Suspect B
Suspect A
Crime Scene
Allele Ladder
The picture below shows results similar to what can be seen following performance of the kit.
15 10 7 5 4 3 2 1
Fig. 8. Representative results from Crime Scene Investigator PCR Basics kit following PCR amplification, gel electrophoresis and gel staining.
The sample in lane 2, the Crime Scene DNA, has two alleles: 7 and 3. The genotype of the Crime Scene DNA is then 7-3 (or 3-7). The genotype of the next sample is 10-3, the next sample is 5-2, and so on. A summary of the alleles identified and the genotypes obtained is in the table below: Lane of gel 1 2 3 4 5 6
DNA sample Allele Ladder Crime Scene Suspect A Suspect B Suspect C Suspect D
genotype (identified alleles) N/A 7-3 (allele 7 + allele 3) 10-3 (allele 10 + allele 3) 5-2 (allele 5 + allele 2) 7-3 (allele 7 + allele 3) 10-2 (allele 10 + allele 2)
The final step in the experiment is to examine the results and determine whether there is a match between the Crime Scene DNA and any of the DNA samples obtained for analysis for the investigation. Samples that don't match the Crime Scene DNA can be excluded from the investigation; samples that do match the Crime Scene DNA are included as part of the suspect pool.
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