ap-bio-1-artificial-selection by Joan Rasmussen

Artificial Selection: Teacher’s Guide

Kit # 3674-01

Captive Care (continued) Planting: • Place arabidopsis seeds in about 1 (continued) mL of fresh water in microfuge tube and place in a refrigerator for 2-4 days (but not longer than a week). This cold snap will improve germination. • Seeds should be sown on top of fluffy soil since they need light to germinate efficiently. Germination should occur in 1-5 days. Carefully monitor moisture until plants get to 4-5 leaf stage (7-10 days after planting). After this time, the tray can dry out periodically. Captive Care (continued) • Fungus gnats and aphids are the most common pests affecting lab-grown arabidopsis. Orthene, at a 1:50 dilution misted onto the Planting: plants can control aphids; gnats are harder to eliminate.

Live material care guide

• Place arabidopsis seeds in about 1 mL of fresh water in microfuge tube and place in a refrigerator for 2-4 days (but not longer than a week). This cold snap will improve germination. Information • Seeds should be sown on top of fluffy soil since they need light to germinate efficiently. Germination should occur in Lfie 1-5Cycle/Span: days. Carefully monitor moisture until plants get to 4-5 leaf stage (7-10 days after planting). After this time, the candicot, dry out • tray Typical 1-5periodically. days for germination. Flowers appear about 3 weeks from planting. Seeds mature about 6 weeks from initial planting. These are the generally self fertilizing, with alab-grown low rate arabidopsis. of cross-fertilization. • Fungus gnats and plants aphids are most common pests affecting Orthene, at a 1:50 dilution misted onto the can control aphids; gnats are harder toineliminate. • plants Cross-fertilization can be accomplished a laboratory, using a paintbrush to transfer pollen from the flower of one plant to the

pistil of the plant to be fertilized.

Information • Arabidopsis is a member of the mustard family (Brassacacaea or Crusiferea) which includes many human food species including Lfieradishes, Cycle/Span: cress, cabbage, broccoli, cauliflower, kale and Brussels sprouts. •• Typical dicot, is 1-5a days germination. Flowers 3 weeks from planting. Seeds mature about 6 weeks from initialtumefaArabidopsis weedfor with a small genome ofappear about about 125 Mb that has been fully sequenced since 2000. Agrobacterium planting. plants generally self fertilizing, with rate ofand cross-fertilization. molecular transformation. Manya low mutants their sequences have been characterized. ciens canThese be used for are • Cross-fertilization can be accomplished in a laboratory, using a paintbrush to transfer pollen from the flower of one plant to the pistil of the plant to be fertilized. • Arabidopsis is a member of the mustard family (Brassacacaea or Crusiferea) which includes many human food species including Wild Habitat radishes, cress, cabbage, broccoli, cauliflower, kale and Brussels sprouts. • Arabidopsis Asia and Africa125 butMb it that is now world-wide as you might for a successful • Arabidopsis isoriginated a weed withina Europe, small genome of about hasfound been fully sequenced since 2000.expect Agrobacterium tumefa- weed. It is consumed by herbivores like rabbits, flea beetles and butterfly or gnat larvae. It is usually an annual plant that can overwinter ciens can be used for molecular transformation. Many mutants and their sequences have been characterized.

in the early part of its vegetative life cycle followed by flowering in the spring.

Disposition Wild Habitat

We do not recommend any and laboratory specimen theworld-wide wild, and as especially notexpect specimens that are not native •• Arabidopsis originated inreleasing Europe, Asia Africa but it is nowinto found you might for a successful weed. It is to the environment. When finished with your plant please disposeorofgnat it bylarvae. incineration in a well-ventilated consumed by herbivores like rabbits, flea beetles and butterfly It is usually an annual plant area. that can overwinter in the early part of its vegetative life cycle followed by flowering in the spring.

Disposition • We do not recommend releasing any laboratory specimen into the wild, and especially not specimens that are not native to the environment. When finished with your plant please dispose of it by incineration in a well-ventilated area.

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