RAPTOR Criteria Table Grading Scale
Attribute Weight (1 to 10)
Description
Singleplex Sensitivity Gram+
10
Limit of detection (Gram + bacteria), B. anthracis
Singleplex Sensitivity Gram-
10
Limit of detection (Gram - bacteria), Y. pestis
Singleplex Sensitivity DNA Virus
10
Singleplex Sensitivity RNA Virus
Attribute
Unit of Measure
Ideal Instrument
Vendor Supplied Information
Hardware Testing Overall Score %
Low (1-3)
Medium (4-7)
High (8-10)
Grade
cfu/ml
Bacteria: 10e7-10e9
Bacteria: 1000-10e6
Bacteria: 1-100
10
cfu/ml
Bacteria: 10e7-10e9
Bacteria: 1000-10e6
Bacteria: 1-100
10
Limit of detection (DNA Virus), Vaccinia
pfu/ml
Virus: 10e7-10e9
Virus: 1000-10e6
Virus: 1-100/mL
10
Limit of detection (RNA Virsu), VEE
pfu/ml
Virus: 10e7-10e9
Virus: 1000-10e6
Singleplex Sensitivity Toxin
10
Limit of detection (Toxin), Botulinum Type A Toxin
mass/mL
Toxins: ug/mL
Multiplex Sensitivity Gram+
5
Limit of detection (Gram + bacteria), B. anthracis
cfu/ml
Bacteria: 10e7-10e9
Multiplex Sensitivity Gram-
5
Limit of detection (Gram - bacteria), Y. pestis
cfu/ml
Bacteria: 10e7-10e9
Multiplex Sensitivity - DNA Virus
5
Limit of detection (DNA Virus), Vaccinia
pfu/ml
Multiplex Sensitivity - RNA Virus
5
Limit of detection (RNA Virsu), VEE
pfu/ml
Multiplex Sensitivity Toxin
5
Limit of detection (Toxin), Botulinum Type A Toxin
Multiplex
9
Number of reportable agents per run
# individual Score = 1 for Score = 5 for Score =8+ for targets per test 1 target 2 targets 3+ targets
Score
Claim
Grade
100
5.00E+04
6
100
No Claim
0
10
100
1.00E+05
Virus: 1-100/mL
10
100
Toxins: ng/mL
Toxins: pg/mL
10
Bacteria: 1000-10e6
Bacteria: 1-100
10
Bacteria: 1000-10e6
Bacteria: 1-100
Virus: 10e7-10e9
Virus: 1000-10e6
Virus: 10e7-10e9
mass/mL # of agents per run
Score
(Wt x Grade)
Grade
60
>5,000,000
1
10
0
50,000,000
3
30
6
60
>100000000
1
10
N/a
0
0
N/a
0
0
100
10ng/mL
7
70
> 10,000 ng/mL
2
20
50
1.00E+06
#VALUE!
0
0
10
50
2.50E+05
#VALUE!
0
0
Virus: 1-100/mL
10
50
1.00E+06
#VALUE!
0
0
Virus: 1000-10e6
Virus: 1-100/mL
10
50
1.00E+08
#VALUE!
0
0
Toxins: ug/mL
Toxins: ng/mL
Toxins: pg/mL
10
50
50 ng/mL
0
0
1 = 0 score; 2-7
8-19
20-34+
10
90
4
2
18
4
2
18
(Wt x Grade)
#VALUE!
(Wt x Grade)
Multiplex
9
Number of individual targets per test
Batch Processing
9
If looking for a single agent, the number of samples that can be processed at the same time by one analyzer.
# of samples
1 = 0 score; 2-9
Boot Up
2
Time required for warm-up and calibration prior to sample analysis
minutes
14+
Sample Preparation Time
8
Time required from receipt of sample until it is ready to be analyzed.
minutes
35+
Analysis Time
9
Time required to provide a test answer after the analysis has been initiated.
minutes
35+
15<35
Sample Preparation
Info Only
Sample preparation required?
Yes/No
Total Analysis Time
Info Only
The duration of time to provide final identification of target from raw data.
minutes
Weight
10
Approximate weight of instrument with power source
lbs
24-36
8<24
2<8
10
100
12.3
5
50
12.3
10
100
Instrument Size
9
square feet
3.5+ sqft
1.5<3.5 sqft
0 <1.5 sq ft
10
90
1
8
72
1
8
72
Logistical Footprint
Info Only
Approximate footprint of instrument only in operational mode Approximate footprint of instrument with ancillary equipment Approximate weight of ancillary equipment (microcentrifuge, plate shaker, plate washer, laptop)
10
90
4
4
36
4
4
36
50+
10
90
1
0
0
1
0
0
6<14
0<6
10
20
0
10
20
0
10
20
15<35
0<15
10
80
0
10
80
0
10
80
0<15
10
90
15
7
63
28
5
45
10-50
Informational only
No
Informational only
Approximately 28 minutes i(ncludes time required for baseline correction (required for a new assay coupon))
14
0
20
76
91 square feet
Ancillary Equipment Weight
Info Only
Power Requirements
8
Battery Supplied
Yes/No
Power Requirements
5
Expected power required
Watts
Informational only
lbs
Less than 0.5 sq. ft
Informational only No = 0; Yes = 10
About 1 lb. 10
80
10
50
Yes
10
80
Yes
10
80
#VALUE! 700+
301-700
0-300
#VALUE!
#VALUE!
User Maintenance
Info Only
Time required for Preventative Maintenance
hours per year
Informational only
Unknown
Ancillary Equipment
Info Only
List ancillary equipment requirement for operation of this instrument
List ancillary equipment
Informational only
A/C Adapter, battery charger
Storage area required for consumables
sqft
Informational only
Each kit measures 7.125 x 7.25 inches (0.36 sq. ft)
Info Only
Special conditions required for consumables (e.g. refrigeration, high humidity).
conditions
Informational only
Refrigeration
Open Architecture
Info Only
Multiple sources of consumables or self-designed assays
Reagent sources (List)
Informational only
No
Informational only
Approximately 10mL per test.
Waste Management
Info Only
Approximate volume of waste streams
Quoted Cost
Info Only
System cost as quoted or listed
$
Informational only
Purchase Cost
Info Only
Actual system cost as purchased
$
Informational only
Reagent Cost
Info Only
Reagent cost per sample
$
Informational only
Service Cost
Info Only
Cost for yearly service.
$
Informational only
Unknown
Compatibility/ Interchangeability
Info Only
Interoperability
Info Only
Types of data files for export (e.g., ASCII, XML, tab delimited text)
File types (list)
Informational only
Proprietary software required to download files.
Usability
Consumables Consumables Requirements
Approximate mL per test
Decontamination Requirements
Info Only
Is decontamination required?
Yes/No (If Yes, internal and/or external ?)
Informational only
Yes. Internal system components are exposed to test agent.
Readiness Level
Info Only
Current Technical Readiness Level
Technology Readiness Level (TRL)
Informational only
Based on SME feedback the TRL is
Costs
Score
Result
Maturity
Logistical Support
Power
Size
Run Time
Flexibility
Target Identification
Category
GRAND TOTAL #VALUE!
130