Catalogo Veterinaria by ayleen lissette sanhueza romero

VetPCRTM AERO.HYDRO Detection Kit

Product

VetPCR AERO.HYDRO Detection Kit

DESCRIPTION Aeromonas hydrophila is a ubiquitous Gram-negative, motile, rod-shaped bacterium which can be commonly isolated from fresh water ponds and is also a normal inhabitant of the gastrointestinal tract of fish. The disease primarily affects freshwater fish such as catfish and bass, and many species of tropical or ornamental fish. Fish infected with A. hydrophila may have many different clinical signs. These range from sudden death in otherwise healthy fish to inappetence, swimming abnormalities, pale gills, bloat and skin ulcerations. The skin ulcers may occur at any site on the fish and often they are surrounded by a bright red rim of tissue. Because of the variability of these clinical signs, the diagnosis of this disease based only upon the clinical presentation of the fish is highly unreliable.

Cod. Kit

48 & 96 Test/kit VET0001F(48) or (96)

Contents TM

VetPCR AERO.HYDRO PCR Pre-mixture DNase/RNase-free water AERO.HYDRO PCR Positive control AERO.HYDROPCR Positive control Pre-mixture BrigTM Molecular Weight marker Mineral Oil DNA purification kit (see step 6.1)

Kit 48

Kit 96

48 1 1 4 1 1 50

96 1 1 8 1 1 100

Package

Characteristics Ready to use : only DNA template and D.W. are needed Easy and speed protocol Stable for 1 year at -20ยบC Time-saving and cost-effective

Storage Condition Store at -20ยบC

VetPCRTM AERO.HYDRO Detection Kit is the direct detection of Aeromonas hydrophila on the basis of a genetic database, so it can diagnose very fast and accurately. It can amplify only specific gene using the PCR (Polymerase Chain Reaction) method, and take only 3 hours for detection. Therefore, it is a very fast, accurate and reliable technique.

Expected PCR product size : 475 bp M

AQUACULTURE

500bp

475 bp

Fig1. Electrophoresis of PCR product by VetPCRTM AERO.HYDRO Detection Kit Lane M : BrigTM Molecular Weight Marker (Bioingentech Ltda.) Lane P : Positive control Lane 1~3 : AERO.HYDRO Positive sample Lane N : Negative control

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VetPCRTM EHNV Detection Kit

Product

VetPCR EHNVDetection Kit

DESCRIPTION Epizootic Haematopoietic Necrosis (EHN) is caused by a double-stranded DNA, non-enveloped Iridovirus known as Epizootic Haematopoeitic Necrosis Virus (EHNV). This virus shares at least one antigen with iridoviruses infecting sheat fish (Silurus glanis) and the cat-fish (Ictalurus melas) in Europe and with amphibian iridoviruses from North America (frog virus 3) and Australia (Bohle iridovirus). Recently, the OIE included the two agents, European catfish virus and European sheatfish virus, as causative agents of EHN. There are no specific clinical signs associated with EHN. Mortalities are characterised by necrosis of liver (with or without white spots), spleen, haematopoietic tissue of the kidney and other tissues. Disruption of blood function leads to osmotic imbalance, haemorrhagic lesions, build up of body fluids in the body cavity. The body cavity fluids (ascites) plus enlarged spleen and kidney may cause abdominal distension (dropsy). Clinical disease appears to be associated with poor water quality, as well as water temperature. In rainbow trout, disease occurs at temperatures from 11to 17°C (in nature). No disease is found in redfin perch at temperatures below 12°C under natural conditions. Both juvenile and adult redfin perch can be affected, but juveniles appear more susceptible.

Cod. Kit

48 & 96 Test/kit VET0001F(48) or (96)

Contents VetPCRTM EHNV PCR Pre-mixture DNase/RNase-free water EHNV PCR Positive control EHNV PCR Positive control Pre-mixture BrigTM Molecular Weight marker Mineral Oil DNA purification kit (see step 6.1)

Kit 48

Kit 96

48 1 1 4 1 1 50

96 1 1 8 1 1 100

Characteristics Ready to use : only DNA template and D.W. are needed Easy and speed protocol Stable for 1 year at -20ºC Time-saving and cost-effective

Storage Condition Store at -20ºC Expected PCR product size : 465 bp M

AQUACULTURE

500bp

VetPCRTM EHNV Detection Kit is the direct detection of Epizootic Haematopoietic Necrosis Virus on the basis of a genetic database, so it can diagnose very fast and accurately. It can amplify only specific gene using the PCR (Polymerase Chain Reaction) method, and take only 3 hours for detection. Therefore, it is a very fast, accurate and reliable technique.

465 bp

Fig1. Electrophoresis of PCR product by VetPCRTM EHNV Detection Kit Lane M : BrigTM Molecular Weight Marker (Bioingentech Ltda.) Lane N : Negative control Lane P : Positive control Lane 1~4 : EHNV Positive sample

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VetPCRTM FLAV.COLUM Detection Kit

Product

VetPCR FLAV.COLUM Detection Kit

DESCRIPTION Flavobacterium columnare is a thin Gram-negative rod bacterium of the genus Flavobacterium. Fish with columnaris usually have brown to yellowish-brown lesions (sores) on their gills, skin and/or fins. The bacteria attach to the gill surface, grow in spreading patches, and eventually cover individual gill filaments. This results in cell death. Portions of the gills are eroded by protein and cartilage-degrading enzymes produced by the bacteria. Skin lesions produced by columnaris initially are very shallow. More advanced lesions may be round or oval in shape, yellowish-brown in color, with an open ulcer in the center. A characteristic lesion produced by columnaris is a pale white band encircling the body, often referred to as saddleback condition. As the infection progresses, a yellowish-brown ulcer often is found in the center of the “saddle”. VetPCRTM FLAV.COLUM Detection Kit is the direct detection of Flavobacterium columnare on the basis of a genetic database, so it can diagnose very fast and accurately. It can amplify only specific gene using the PCR (Polymerase Chain Reaction) method, and take only 3 hours for detection. Therefore, it is a very fast, accurate and reliable technique.

Cod. Kit

48 & 96 Test/kit VET0001F(48) or (96)

Contents

Kit 48

Kit 96

48 1 1 4 1 1 50

96 1 1 8 1 1 100

VetPCR FLAV.COLUM PCR Pre-mixture DNase/RNase-free water FLAV.COLUM PCR Positive control FLAV.COLUMPCR Positive control Pre-mixture BrigTM Molecular Weight marker Mineral Oil DNA purification kit (see step 6.1)

Characteristics Ready to use : only DNA template and D.W. are needed Easy and speed protocol Stable for 1 year at -20ºC Time-saving and cost-effective

Storage Condition Store at -20ºC Expected PCR product size : 667 bp M

700bp

AQUACULTURE

Package

667 bp

Fig1. Electrophoresis of PCR product by VetPCRTM FLAV.COLUM Detection Kit Lane M : BrigTM Molecular Weight Marker (Bioingentech Ltda.) Lane P : Positive control Lane 1~4 : FLAV.COLUM Positive sample Lane N : Negative control

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VetPCRTM KUDOA.sp Detection Kit

Product

VetPCR KUDOA.sp Detection Kit

DESCRIPTION

AQUACULTURE

Kudoa sp. is a myxosporean parasite. The majority of Kudoa species infect the somatic muscle of marine and estuarine fish establishing cysts, which contain many spores. As the parasite grows, it produces proteolytic enzymes that break down the filaments of the muscle fibre. While the parasite is within a muscle fibre, it is undetected by the host’s immunological system. It is during this stage when the parasite contains many developing and mature spores, that the infected fibres have a white appearance. As the parasite grows, it breaks the sarcolemma and the host recognizes the presence of the parasite. Then, there is a rapid development of a fibroblast layer around the parasite and the cyst, more properly, pseudocyst, quickly acquires a black appearance. Deterioration occurs rapidly after salmon are killed and there is no known cure. Kudoa contamination is usually first detected when salmon are slaughtered and processed. This parasite is responsible for causing economic losses to the fisheries sector, by causing post-mortem “myoliquefaction”, a softening of the flesh to such an extent that the fish becomes unmarketable. It is not infective to humans. VetPCRTM KUDOA.sp Detection Kit is the direct detection of Kudoa sp. on the basis of a genetic database, so it can diagnose very fast and accurately. It can amplify only specific gene using the PCR (Polymerase Chain Reaction) method, and take only 3 hours for detection. Therefore, it is a very fast, accurate and reliable technique.

Cod. Kit

48 & 96 Test/kit VET0001F(48) or (96)

Contents

Kit 48

Kit 96

48 1 1 4 1 1 50

96 1 1 8 1 1 100

VetPCR KUDOA.sp PCR Pre-mixture DNase/RNase-free water KUDOA.sp PCR Positive control KUDOA.sp PCR Positive control Pre-mixture BrigTM Molecular Weight marker Mineral Oil DNA purification kit (see step 6.1)

Characteristics Ready to use : only DNA template and D.W. are needed Easy and speed protocol Stable for 1 year at -20ºC Time-saving and cost-effective

Storage Condition Store at -20ºC Expected PCR product size : 277 bp M

300bp

277 bp

Fig1. Electrophoresis of PCR product by VetPCRTM KUDOA.sp Detection Kit Lane M : BrigTM Molecular Weight Marker (Bioingentech Ltda.) Lane P : Positive control Lane 1~3 : KUDOA.sp Positive sample Lane N : Negative control

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VetPCRTM NUCLE.SAL Detection Kit

Product

VetPCR NUCLE.SAL Detection Kit

DESCRIPTION Nucleospora salmonis a member of the family Enterocytozoonidae (Apansporoblastina), is an intranuclear microsporidian parasite of marine and freshwater fish, causes diseases mainly in salmonid species. Intranuclear stages of development have been reported for N. salmonis in the nuclei of lymphoblasts and plasmablasts. Nucleospora-like parasites have also been described in nonsalmonid fish. N. salmonis is associated with chronic mortality in several salmonid fish species and may induce a disease characterized by anemia and severe lymphoblastosis accompanied by a leukemic-like state. The disease associated with N. salmonis is confirmed by observation of clinical signs that include exophthalmos, renal hypertrophy and splenomegaly, and swelling of the large intestine and by identification of characteristic developmental stages or spores, distinct from nucleoli or vacuoles, within the nucleus of the host cell. VetPCRTM NUCLE.SAL Detection Kit is the direct detection of Nucleospora salmonis on the basis of a genetic database, so it can diagnose very fast and accurately. It can amplify only specific gene using the PCR (Polymerase Chain Reaction) method, and take only 3 hours for detection. Therefore, it is a very fast, accurate and reliable technique.

48 & 96 Test/kit

Contents

Cod. Kit VET0001F(48) or (96)

Kit 48

Kit 96

48 1 1 4 1 1 50

96 1 1 8 1 1 100

VetPCRTM NUCLE.SAL PCR Pre-mixture DNase/RNase-free water NUCLE.SAL PCR Positive control NUCLE.SAL PCR Positive control Pre-mixture BrigTM Molecular Weight marker Mineral Oil DNA purification kit (see step 6.1)

Characteristics Ready to use : only DNA template and D.W. are needed Easy and speed protocol Stable for 1 year at -20ยบC Time-saving and cost-effective

Storage Condition Store at -20ยบC Expected PCR product size : 237 bp M

AQUACULTURE

300bp

237 bp

Fig1. Electrophoresis of PCR product by VetPCRTM NUCLE.SAL Detection Kit Lane M : BrigTM Molecular Weight Marker (Bioingentech Ltda.) Lane N : Negative control Lane 1~3 : NUCLE.SAL Positive sample Lane P : Positive control

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VetPCRTM PISRIC.SAL Detection Kit

Product

VetPCR PISRIC.SAL Detection Kit

AQUACULTURE

DESCRIPTION Piscirickettsia salmonis is a gram-negative obligate intracellular bacterium that causes systemic septicaemia (salmonid rickettsial syndrome, SRS, or piscirickettsiosis) in salmonid fish. Piscirickettsia-like bacteria are now been recognized with increasing frequency in a variety of other fish species, from both fresh and salt waters around the world. Piscirickettsiosis and piscirickettsiosis-like diseases have affected aquaculture productivity, profitability, the species compatible with commercial rearing, and transportation of fish from site to site. The Chilean aquaculture industry alone attributes annual losses to salmonid piscirickettsiosis of $150 million. In Chile, the syndrome has led to a shift from the more commercially desirable coho salmon to the less desirable but more piscirickettsiosis resistant Atlantic salmon as the primary cultivated species. Clinical signs include; dark in color, and swam at the edges of the pens near the surface. Gross pathology include swollen kidneys and enlarged spleens. Other characteristics seen in some affected fish are anemia (hematocrit 27 % or less), ascites, mottled livers with grey circular lesions, and petechial hemorrhages in the stomach, intestines, pyloric caeca, and visceral fat. VetPCRTM PISCI.SAL Detection Kit is the direct detection of Piscirickettsia salmonis on the basis of a genetic database, so it can diagnose very fast and accurately. It can amplify only specific gene using the PCR (Polymerase Chain Reaction) method, and take only 3 hours for detection. Therefore, it is a very fast, accurate and reliable technique.

Cod. Kit

48 & 96 Test/kit VET0001F(48) or (96)

Contents

Kit 48

Kit 96

48 1 1 4 1 1 50

96 1 1 8 1 1 100

VetPCR PISRIC.SAL PCR Pre-mixture DNase/RNase-free water PISRIC.SAL PCR Positive control PISRIC.SAL PCR Positive control Pre-mixture BrigTM Molecular Weight marker Mineral Oil DNA purification kit (see step 6.1)

Characteristics Ready to use : only DNA template and D.W. are needed Easy and speed protocol Stable for 1 year at -20ยบC Time-saving and cost-effective

Storage Condition Store at -20ยบC Expected PCR product size : 325 bp M

400bp

325 bp

Fig1. Electrophoresis of PCR product by VetPCRTM PISRIC.SAL Detection Kit Lane M : BrigTM Molecular Weight Marker (Bioingentech Ltda.) Lane P : Positive control Lane 1~3 : PISRIC.SAL Positive sample Lane N : Negative control

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VetPCRTM RENI.SAL Detection Kit

Product

VetPCR RENI.SAL Detection Kit

DESCRIPTION

AQUACULTURE

Bacterial kidney disease (BKD) is caused by Renibacterium salmoninarum, a coryne form, rod-shaped, Gram-positive bacterium that is the sole species belonging to the genus Renibacterium. BKD occurs in North America, Japan, Western Europe and Chile. R. salmoninarum infections can buildup over a long period of time, with clinical disease only appearing in advanced infections, usually when the fish have completed their first year of life. Virulence of R. salmoninarum varies with: the strain of bacterium the salmon species infected environmental and holding conditions. The bacteria can evade lysosomal breakdown by the blood cells that engulf them, thus avoid destruction by the fishes’ primary defence mechanism. Nutrition and seawater transfers can also affect the pathogenicity of R. salmoninarum infections and brood stock infection levels are believed to have a direct correlation to susceptibility in their offspring. Progeny of parent stock with low levels or no infection with R. salmoninarum show better survival than offspring from BKD compromised fish. This may reflect greater transmission titres by the latter. VetPCRTM RENI.SAL Detection Kit is the direct detection of Renibacterium salmoninarum on the basis of a genetic database, so it can diagnose very fast and accurately. It can amplify only specific gene using the PCR (Polymerase Chain Reaction) method, and take only 3 hours for detection. Therefore, it is a very fast, accurate and reliable technique.

48 & 96 Test/kit

Contents

Cod. Kit VET0001F(48) or (96)

Kit 48

Kit 96

48 1 1 4 1 1 50

96 1 1 8 1 1 100

VetPCRTM RENI.SAL PCR Pre-mixture DNase/RNase-free water RENI.SAL PCR Positive control RENI.SAL PCR Positive control Pre-mixture BrigTM Molecular Weight marker Mineral Oil DNA purification kit (see step 6.1)

Characteristics Ready to use : only DNA template and D.W. are needed Easy and speed protocol Stable for 1 year at -20ºC Time-saving and cost-effective

Storage Condition Store at -20ºC Expected PCR product size : 544 bp M

600bp

544 bp

Fig1. Electrophoresis of PCR product by VetPCRTM RENI.SAL Detection Kit Lane M : BrigTM Molecular Weight Marker (Bioingentech Ltda.) Lane P : Positive control Lane 1~4 : RENI.SAL Positive sample Lane N : Negative control

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VetPCRTM STREP.PHOC Detection Kit

Product

VetPCR STREP.PHOC Detection Kit

DESCRIPTION Streptococcus phocae a Gram-positive, catalase-negative cocci, was originally associated with pneumonia and sinusitis in harbour seals (Phoca vitulina) infected with morbillivirus in north western Europe, and later was attributed to septicemia, emaciation and abortion in cape fur seals in Namibia and South Africa. Infection with S. phocae has been described in harbour seals (Phoca vitulina) and grey seals (Haliochoerus grypus), both of which belong to the family Phocidae or true seals. The reported cases of S. phocae infection were from seals originating in the north Atlantic Ocean, from the coastline of the British Isles, Scandinavia, the Netherlands and Germany. VetPCRTM STREP.PHOC Detection Kit is the direct detection of Streptococcus phocae on the basis of a genetic database, so it can diagnose very fast and accurately. It can amplify only specific gene using the PCR (Polymerase Chain Reaction) method, and take only 3 hours for detection. Therefore, it is a very fast, accurate and reliable technique.

Cod. Kit

48 & 96 Test/kit VET0001F(48) or (96)

Contents TM

VetPCR STREP.PHOC PCR Pre-mixture DNase/RNase-free water STREP.PHOC PCR Positive control STREP.PHOC PCR Positive control Pre-mixture BrigTM Molecular Weight marker Mineral Oil DNA purification kit (see step 6.1)

Kit 48

Kit 96

48 1 1 4 1 1 50

96 1 1 8 1 1 100

Characteristics Ready to use : only DNA template and D.W. are needed Easy and speed protocol Stable for 1 year at -20ยบC Time-saving and cost-effective

Storage Condition Store at -20ยบC Expected PCR product size : 278 bp M

AQUACULTURE

300bp

278 bp

Fig1. Electrophoresis of PCR product by VetPCRTM STREP.PHOC Detection Kit Lane M : BrigTM Molecular Weight Marker (Bioingentech Ltda.) Lane P : Positive control Lane 1~3 : STREP.PHOC Positive sample Lane N : Negative control

Package tubes vial vial tubes vial vial tests

VetPCRTM RENI.SAL Detection Kit

Product

VetPCR RENI.SAL Detection Kit

DESCRIPTION

AQUACULTURE

48 & 96 Test/kit

Contents

Cod. Kit VET0001F(48) or (96)

Kit 48

Kit 96

48 1 1 4 1 1 50

96 1 1 8 1 1 100

Characteristics Ready to use : only DNA template and D.W. are needed Easy and speed protocol Stable for 1 year at -20ºC Time-saving and cost-effective

Storage Condition Store at -20ºC Expected PCR product size : 544 bp M

600bp

544 bp

Package tubes vial vial tubes vial vial tests

VetPCRTM VIBRIO.spp Detection Kit

Product

VetPCR VIBRIO.spp Detection Kit

DESCRIPTION Vibriosis is a major disease problem in shrimp aquaculture, causing high mortalities and severe economic losses in all producing countries. It is most often considered as an opportunistic pathology in shrimp, but a primary disease caused by highly virulent strains of Vibrio spp. has also been reported. The major genospecies causing vibriosis in shrimp are Vibrio harveyi, V. alginolyticus, V. parahaemolyticus, V. anguillarum. These pathologies occur at all rearing levels, from hatchery tanks to growout ponds. However, specificity has been reported both at the species and the stage level, so strains responsible for larval and juvenile vibriosis are considered to be different, even when belonging to the same species.

Cod. Kit

48 & 96 Test/kit VET0001F(48) or (96)

Contents

Kit 48

Kit 96

48 1 1 4 1 1 50

96 1 1 8 1 1 100

VetPCR VIBRIO.spp PCR Pre-mixture DNase/RNase-free water VIBRIO.spp PCR Positive control VIBRIO.spp PCR Positive control Pre-mixture BrigTM Molecular Weight marker Mineral Oil DNA purification kit (see step 6.1)

Characteristics Ready to use : only DNA template and D.W. are needed Easy and speed protocol Stable for 1 year at -20ยบC Time-saving and cost-effective

Storage Condition VetPCRTM VIBRIO spp. Detection Kit is the direct detection of Vibrio spp. on the basis of a genetic database, so it can diagnose very fast and accurately. It can amplify only specific gene using the PCR (Polymerase Chain Reaction) method, and take only 3hours for detection. Therefore, it is a very fast accurate and reliable technique.

Store at -20ยบC Expected PCR product size : 309 bp M

AQUACULTURE

400bp

309 bp

Fig1. Electrophoresis of PCR product by VetPCRTM VIBRIO.spp Detection Kit Lane M : BrigTM Molecular Weight Marker (Bioingentech Ltda.) Lane P : Positive control Lane 1~4 : VIBRIO.spp Positive sample Lane N : Negative control

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VetPCRTM WSSV Detection Kit

Product

VetPCR WSSV Detection Kit

DESCRIPTION White spot syndrome virus (WSSV), the sole member of the virus family Nimaviridae, is a large double-stranded DNA virus that infects shrimp and other crustaceans. WSSV first appearing in the 1990s in Taiwan, has spread rapidly to shrimp-farming areas all over the world and become one of the major pathogens in cultured shrimp. It has a broad host range, infecting all cultured shrimp as well as other invertebrate aquatic organisms such as crab and crayfish. The virus can cause 100% accumulative mortality in 2~10 days to farmed shrimp. Therefore, WSSV has attracted intensive investigations, resulting in some exciting research progress in the past decade. Studies on virus replication and viron morphogenesis show that DNA replication and de novo envelope formation take place in the nucleus. However, the mechanism of WSSV entry into the shrimp cell and of the spread of the virus in the crustacean body is still unknown. VetPCRTM WSSV Detection Kit is the direct detection of White spot syndrome virus on the basis of a genetic database, so it can diagnose very fast and accurately. It can amplify only specific gene using the PCR (Polymerase Chain Reaction) method, and take only 3hours for detection. Therefore, it is a very fast accurate and reliable technique.

48 & 96 Test/kit

Contents VetPCRTM WSSV PCR Pre-mixture DNase/RNase-free water WSSV PCR Positive control WSSV PCR Positive control Pre-mixture BrigTM Molecular Weight marker Mineral Oil DNA purification kit (see step 6.1)

Cod. Kit VET0001F(48) or (96)

Kit 48

Kit 96

48 1 1 4 1 1 50

96 1 1 8 1 1 100

Characteristics Ready to use : only DNA template and D.W. are needed Easy and speed protocol Stable for 1 year at -20ยบC Time-saving and cost-effective

Storage Condition Store at -20ยบC Expected PCR product size : 340 bp M

AQUACULTURE

400bp

340 bp

Fig1. Electrophoresis of PCR product by VetPCRTM WSSV Detection Kit Lane M : BrigTM Molecular Weight Marker (Bioingentech Ltda.) Lane N : Negative control Lane P : Positive control Lane 1~4 : WSSV Positive sample

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VetPCRTM YER.RUCKE Detection Kit

Product

VetPCR YER.RUCKE Detection Kit

DESCRIPTION Yersinia ruckeri is a gram-negative, rod shaped bacterial pathogen. It is a member of the Kingdom Eubacteria. Most have a flagellum used for mobility. Yersinia ruckeri is the causative agent of Enteric Redmouth (ERM) disease, found in salmonids, mainly rainbow trout. It is also know as Yersiniosis. Symptoms are: the reddening of the mouth, fish will stop eating and may isolate themselves, the fish become darker and their fins become inflamed, and the eyes become exophthalmic and may burst. The bacteria infect most tissues of the body, mainly well-vascularized areas, and can cause hemorrhaging. This can cause internal organs, such as the spleen, to also redden. ERM is spread from direct contact with contaminated fish or their feces. VetPCRTM YER.RUCKE Detection Kit is the direct detection of Yersinia ruckeri on the basis of a genetic database, so it can diagnose very fast and accurately. It can amplify only specific gene using the PCR (Polymerase Chain Reaction) method, and take only 3 hours for detection. Therefore, it is a very fast, accurate and reliable technique.

Cod. Kit

48 & 96 Test/kit VET0001F(48) or (96)

Contents TM

VetPCR YER.RUCKE PCR Pre-mixture DNase/RNase-free water YER.RUCKE PCR Positive control YER.RUCKE PCR Positive control Pre-mixture BrigTM Molecular Weight marker Mineral Oil DNA purification kit (see step 6.1)

Kit 48

Kit 96

48 1 1 4 1 1 50

96 1 1 8 1 1 100

Characteristics Ready to use : only DNA template and D.W. are needed Easy and speed protocol Stable for 1 year at -20ยบC Time-saving and cost-effective

Storage Condition Store at -20ยบC Expected PCR product size : 364 bp M

AQUACULTURE

400bp

364 bp

Fig1. Electrophoresis of PCR product by VetPCRTM YER.RUCKE Detection Kit Lane M : BrigTM Molecular Weight Marker (Bioingentech Ltda.) Lane P : Positive control Lane 1~3 : YER.RUCKE Positive sample Lane N : Negative control

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VetPCRTM IPNV Detection Kit DESCRIPTION

AQUACULTURE

Infectious pancreatic necrosis (IPN) is caused by a highly contagious virus, Infectious pancreatic necrosis virus (IPNV) belonging to the Birnaviridae. It is a bi-segmented dsRNA virus which occurs primarily in freshwater, but appears to be saltwater tolerant. The disease has a wide geographical distribution, occurring in most, if not all, major salmonid farming countries of North and South America, Europe and Asia. The first sign of IPN in salmonid fry is the sudden onset of mortality. This shows a progressive increase in severity, especially following introduction of feed to post-yolk-sac fry. IPN also affects American salmon smolt shortly after transfer to sea-cages. Clinical signs include darkening of the lower third of the body and small swellings on the head and a pronounced distended abdomen and a corkscrewing/spiral swimming motion. Some fish may also show ‘pop-eye’ deformities. Cumulative mortalities may vary from less than 10% to more than 90% depending on the combination of several factors such as virus strain, host and environment. VetPCRTM IPNV Detection Kit is the direct detection of Infectious pancreatic necrosis virus on the basis of a genetic database, so it can diagnose very fast and accurately. It can amplify only specific gene using the PCR (Polymerase Chain Reaction) method, and take only 3 hours for detection. Therefore, it is a very fast, accurate and reliable technique.

Product

VetPCRTM IPNV Detection Kit

48 & 96 Test/kit

Contents VetPCRTM IPNV RT-PCR Pre-mixture VetPCRTM IPNV PCR Pre-mixture BrigRT-PCRTM solution BiotechTM Transcriptase solution DNase/RNase-free water IPNV RT-PCR Positive control IPNV RT-PCR Positive control Pre-mixture IPNV PCR Positive control Pre-mixture BrigTM Molecular Weight marker Mineral Oil RNA extraction kit (see step 6.1) Characteristics

Kit 48 48 48 1 1 1 1 4 4 1 1 50

Cod. Kit VET0001F(48) or (96) Kit 96 96 96 1 1 1 1 8 8 1 2 100

Ready to use : only DNA template and D.W. are needed Easy and speed protocol Stable for 1 year at -20ºC Time-saving and cost-effective

Storage Condition Store at -20ºC Expected PCR product size : 309bp M P 1 2

300bp

309bp

Fig1. Electrophoresis of PCR product by VetPCRTM IPNV Detection Kit Lane M : BrigTM Molecular Weight Marker (Bioingentech Ltda.) Lane P : Positive control Lane 1~4 : IPNV Positive sample Lane N : Negative control

Package tubes vial vial vial vial vial tubes tubes vial vial(s) tests

VetPCRTM ISAV Detection Kit DESCRIPTION Infectious salmon anemia (ISA) virus is an emerging viral pathogen of farmed Atlantic salmon. The ISA disease, first reported in Norway in 1984 and reached a peak incidence in 1991. The virus has a negative-stranded RNA genome and is an orthomyxovirus belonging to the genus Isavirus, family Orthomyxoviridae. Infectious salmon anemia (ISA) is a severe disease affecting farmed Atlantic salmon with typical pathological changes characterized by severe anemia, leukopenia, ascitic fluids, hemorrhagic liver necrosis, and petecchiae of the viscera. Clinical signs of ISA virus infection can include lethargic fish with pale gills, bulging eyes, hemorrhage in the eye chamber, skin and muscle. Internal signs include abdominal fluid bloating, dark pale, yellowish, or black livers, swollen spleen, patchy hemorrhaging in fat tissues, the swim bladder and muscles. These disease symptoms clearly reduce the market value of infected salmon.

AQUACULTURE

VetPCRTM ISAV Detection Kit is the direct detection of Infectious salmon anemia virus on the basis of a genetic database, so it can diagnose very fast and accurately. It can amplify only specific gene using the PCR (Polymerase Chain Reaction) method, and take only 3 hours for detection. Therefore, it is a very fast, accurate and reliable technique.

Product VetPCRTM ISAV Detection Kit

Product 48 & 96 Test/kit

Contents VetPCRTM ISAV RT-PCR Pre-mixture VetPCRTM ISAV PCR Pre-mixture BrigRT-PCRTM solution BiotechTM Transcriptase solution DNase/RNase-free water ISAV RT-PCR Positive control ISAV RT-PCR Positive control Pre-mixture ISAV PCR Positive control Pre-mixture BrigTM Molecular Weight marker Mineral Oil RNA extraction kit (see step 6.1) Characteristics

Kit 48 48 48 1 1 1 1 4 4 1 1 50

Cod. Kit VET0001F(48) or (96) Kit 96 96 96 1 1 1 1 8 8 1 2 100

Ready to use : only DNA template and D.W. are needed Easy and speed protocol Stable for 1 year at -20ยบC Time-saving and cost-effective

Storage Condition Store at -20ยบC Expected PCR product size : 616 bp M N P 1 2

600bp

616 bp

Fig1. Electrophoresis of PCR product by VetPCRTM ISAV Detection Kit Lane M : BrigTM Molecular Weight Marker (Bioingentech Ltda.) Lane N : Negative control Lane P : Positive control Lane 1~4 : ISAV Positive sample

Package tubes vial vial vial vial vial tubes tubes vial vial(s) tests

VetPCRTM NWV Detection Kit DESCRIPTION

AQUACULTURE

Noroviruses (Norwalk and Norwalk-like viruses) are known to be one of the major pathogenic viruses implicated in outbreaks of gastroenteritis caused by feeding of seafood. Norwalk virus contain a positive strand RNA genome of 7.5 kb and a single structural protein of about 60 kDa. It is supposed that Norwalk virus would be emitted into water environment through sewage and the shellfish growing in polluted water environment would enrich with contaminated virus particles in their body. The most common symptoms of Norwalk virus infection are diarrea and/ or vomiting that may be accompanied by abdominal pains, myalgia, headache and sometimes mild fever. In most cases people who are otherwise healthy recover from the infection within one to three days without any treatment. Those infected with norovirus disease should not cook or serve food to others. Thorough handwashing and hygiene are always essential and the most effective means of preventing transmission. VetPCRTM NWV Detection Kit is the direct detection of Norwalk virus on the basis of a genetic database, so it can diagnose very fast and accurately. It can amplify only specific gene using the PCR (Polymerase Chain Reaction) method, and take only 3 hours for detection. Therefore, it is a very fast, accurate and reliable technique.

Product

VetPCRTM NWV Detection Kit

48 & 96 Test/kit

Contents VetPCRTM NWV RT-PCR Pre-mixture VetPCRTM NWV PCR Pre-mixture BrigRT-PCRTM solution BiotechTM Transcriptase solution DNase/RNase-free water NWV RT-PCR Positive control NWV RT-PCR Positive control Pre-mixture NWV PCR Positive control Pre-mixture BrigTM Molecular Weight marker Mineral Oil RNA extraction kit (see step 6.1) Characteristics

Kit 48 48 48 1 1 1 1 4 4 1 1 50

Cod. Kit VET0001F(48) or (96) Kit 96 96 96 1 1 1 1 8 8 1 2 100

Ready to use : only DNA template and D.W. are needed Easy and speed protocol Stable for 1 year at -20ยบC Time-saving and cost-effective

Storage Condition Store at -20ยบC Expected PCR product size : 551 bp M

600bp

551 bp

Fig1. Electrophoresis of PCR product by VetPCRTM NWV Detection Kit Lane M : BrigTM Molecular Weight Marker (Bioingentech Ltda.) Lane N : Negative control Lane 1~3 : NWV Positive sample Lane P : Positive control

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VetPCRTM SPDV Detection Kit DESCRIPTION Pancreatic Disease (PD) is a serious viral disease that affects fish, in particular salmonid fish such as wild Atlantic salmon, rainbow trout and the like. The disease causes lesions in the pancreas, including loss of pancreatic exocrine tissue, and fibrosis, cardiac and skeletal muscle myopathies. Outbreaks of PD were first was first recorded in Scotland in 1976. PD typically affects the fish post-molts during the first year after they are transferred to sea sites and is reported to spread rapidly among farm fish held in sea cages. Clinical signs include lethargy with a tendency to congregate in cage corners and to fail to maintain a horizontal position, cessation of feeding (anorexia) and significant mortalities confirmed these observations in a later study, in which it was found that cardiac and skeletal myopathy is exacerbated in fish suffering from PD.

AQUACULTURE

VetPCRTM SPDV Detection Kit is the direct detection of Salmon Pancreatic disease virus on the basis of a genetic database, so it can diagnose very fast and accurately. It can amplify only specific gene using the PCR (Polymerase Chain Reaction) method, and take only 3 hours for detection. Therefore, it is a very fast, accurate and reliable technique.

Product

VetPCRTM SPDV Detection Kit

48 & 96 Test/kit

Contents VetPCRTM SPDV RT-PCR Pre-mixture VetPCRTM SPDV PCR Pre-mixture BrigRT-PCRTM solution BiotechTM Transcriptase solution DNase/RNase-free water SPDV RT-PCR Positive control SPDV RT-PCR Positive control Pre-mixture SPDV PCR Positive control Pre-mixture BrigTM Molecular Weight marker Mineral Oil RNA extraction kit (see step 6.1) Characteristics

Kit 48 48 48 1 1 1 1 4 4 1 1 50

Cod. Kit VET0001F(48) or (96) Kit 96 96 96 1 1 1 1 8 8 1 2 100

Ready to use : only DNA template and D.W. are needed Easy and speed protocol Stable for 1 year at -20ยบC Time-saving and cost-effective

Storage Condition Store at -20ยบC Expected PCR product size : 542 bp M

600bp

542bp

Fig1. Electrophoresis of PCR product by VetPCRTM SPDV Detection Kit Lane M : BrigTM Molecular Weight Marker (Bioingentech Ltda.) Lane P : Positive control Lane 1~4 : SPDV Positive sample Lane N : Negative control

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VetPCRTM VHSV Detection Kit DESCRIPTION Viral haemorrhagic septicaemia (VHS) is caused by ssRNA enveloped rhabdovirus, known as viral haemorrhagic septicaemia virus (VHSV). VHSV is synonymous with Egtved virus. VHSV is found in continental Europe, the Atlantic Ocean and Baltic Sea. Although VHSV-like infections are emerging in wild marine fish in North America, VHS continues to be considered a European-based disease, until the phylogenetic identities of the VHSVlike viruses which do not cause pathology in rainbow trout can be clearly established. The virus infects blood cells (leucocytes), the endothelial cells of the blood capillaries, haematopoietic cells of the spleen, heart, nephron cells of the kidney, parenchyma of the brainand the pillar cells of the gills. Spread of the virus causes haemorrhage and impairment of osmoregulation. This is particularly severe in juvenile fish, especially during periods when water temperatures ranging between 4 – 14 °C.

AQUACULTURE

VetPCRTM VHSV Detection Kit is the direct detection of Viral haemorrhagic septicaemia virus on the basis of a genetic database, so it can diagnose very fast and accurately. It can amplify only specific gene using the PCR (Polymerase Chain Reaction) method, and take only 3 hours for detection. Therefore, it is a very fast, accurate and reliable technique.

Product

VetPCRTM VHSV Detection Kit

48 & 96 Test/kit

Contents VetPCRTM VHSV RT-PCR Pre-mixture VetPCRTM VHSV PCR Pre-mixture BrigRT-PCRTM solution BiotechTM Transcriptase solution DNase/RNase-free water VHSV RT-PCR Positive control VHSV RT-PCR Positive control Pre-mixture VHSV PCR Positive control Pre-mixture BrigTM Molecular Weight marker Mineral Oil RNA extraction kit (see step 6.1) Characteristics

Kit 48 48 48 1 1 1 1 4 4 1 1 50

Cod. Kit VET0001F(48) or (96) Kit 96 96 96 1 1 1 1 8 8 1 2 100

Ready to use : only DNA template and D.W. are needed Easy and speed protocol Stable for 1 year at -20ºC Time-saving and cost-effective

Storage Condition Store at -20ºC Expected PCR product size : 376bp M

400bp

376bp

Fig1. Electrophoresis of PCR product by VetPCRTM VHSV Detection Kit Lane M : BrigTM Molecular Weight Marker (Bioingentech Ltda.) Lane N : Negative control Lane 1~4 : VHSV Positive sample Lane P : Positive control

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VetPCRTM VNNV Detection Kit

AQUACULTURE

DESCRIPTION The organism responsible for causing viral nervous necrosis (VNN) or viral encephalopathy and retinopathy (VER) has been demonstrated to belong to the group Nodaviridae and is an icosahedral, non-enveloped virus, 2530 nm in diameter. These agents are also known as Striped Jack Nervous Necrosis Virus (SJNNV) and Fish Encephalitis Virus (FEV). All share serological similarities with the exception of those affecting turbot. VNN occurs in Asia, the Mediterranean and the Pacific. VNN affects the nervous system. All affected species show abnormal swimming behaviour (cork-screwing, whirling, darting and belly-up motion) accompanied by variable swim bladder hyperinflation, cessation of feeding, changes in colouration, and mortality. Differences between species are most apparent with relation to age of onset and clinical severity. Earlier clinical onset is associated with greater mortality, thus onset at one day post-hatch in striped jack results in more severe losses than suffered by turbot, where onset is up to three weeks post-hatch. Mortalities range from 10-100%.Two forms of VER have been induced with experimental challenges. Acute - induced by intramuscular inoculation. Sub-acute - by intraperitoneal inoculation, bath, cohabitation and oral routes. VetPCRTM VNNV Detection Kit is the direct detection of Viral nervous necrosis virus on the basis of a genetic database, so it can diagnose very fast and accurately. It can amplify only specific gene using the PCR (Polymerase Chain Reaction) method, and take only 3 hours for detection. Therefore, it is a very fast, accurate and reliable technique.

Product

VetPCRTM VNNV Detection Kit

48 & 96 Test/kit

Contents VetPCRTM VNNV RT-PCR Pre-mixture VetPCRTM VNNV PCR Pre-mixture BrigRT-PCRTM solution BiotechTM Transcriptase solution DNase/RNase-free water VNNV RT-PCR Positive control VNNV RT-PCR Positive control Pre-mixture VNNV PCR Positive control Pre-mixture BrigTM Molecular Weight marker Mineral Oil RNA extraction kit (see step 6.1) Characteristics

Kit 48 48 48 1 1 1 1 4 4 1 1 50

Cod. Kit VET0001F(48) or (96) Kit 96 96 96 1 1 1 1 8 8 1 2 100

Ready to use : only DNA template and D.W. are needed Easy and speed protocol Stable for 1 year at -20ยบC Time-saving and cost-effective

Storage Condition Store at -20ยบC Expected PCR product size : 580 bp M

600bp

580 bp

Fig1. Electrophoresis of PCR product by VetPCRTM VNNV Detection Kit Lane M : BrigTM Molecular Weight Marker (Bioingentech Ltda.) Lane 1~3 : VNNV Positive sample Lane P : Positive control Lane N : Negative control

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VetPCRTM APEC Detection Kit

Product

VetPCR APEC Detection Kit

DESCRIPTION Escherichia coli is a Gram-negative, rod-shaped bacterium normally found in the intestines of poultry and most other animals; although most are nonpathogenic, a limited number produce extraintestinal infections. Pathogenic strains are most commonly of the 02, 078, and 01 serotypes, but a large number of others also produce disease. Virulence factors include the ability to resist phagocytosis, utilization of highly efficient iron acquisition systems, resistance to killing by serum, production of colicins, and adherence to respiratory epithelium. Large numbers of E. coli are maintained in the poultry house environment through fecal contamination. Initial exposure to pathogenic E. coli may occur in the hatchery from infected or contaminated eggs, but systemic infection usually requires predisposing environmental or infectious causes. Mycoplasmosis, infectious bronchitis, Newcastle disease, hemorrhagic enteritis, and turkey bordetellosis are often complicated by colibacillosis. Bacteremia progresses to septicemia and death, or the infection extends to serosal surfaces, pericardium, joints, and other organs.

AVIAN

VetPCRTM APEC Detection Kit is the direct detection of Avian pathogenic Escherichia coli on the basis of a genetic database, so it can diagnose very fast and accurately. It can amplify only specific gene using the PCR (Polymerase Chain Reaction) method, and take only 3 hours for detection. Therefore, it is a very fast, accurate and reliable technique.

48 & 96 Test/kit

Contents

Cod. Kit VET0001F(48) or (96)

Kit 48

Kit 96

48 1 1 4 1 1 50

96 1 1 8 1 1 100

VetPCR APEC PCR Pre-mixture DNase/RNase-free water APEC PCR Positive control APEC PCR Positive control Pre-mixture BrigTM Molecular Weight marker Mineral Oil DNA purification kit (see step 6.1)

Characteristics Ready to use : only DNA template and D.W. are needed Easy and speed protocol Stable for 1 year at -20ยบC Time-saving and cost-effective

Storage Condition Store at -20ยบC Expected PCR product size : 584 bp M

600bp

584 bp

Fig1. Electrophoresis of PCR product by VetPCRTM APEC Detection Kit Lane M : BrigTM Molecular Weight Marker (Bioingentech Ltda.) Lane 1~3 : APEC Positive sample Lane P : Positive control Lane N : Negative control

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VetPCRTM APV Detection Kit

Product

VetPCR APV Detection Kit

DESCRIPTION Avian polyomavirus (APV) causes budgerigar fledgling disease. The virus has a double-stranded DNA genome. It has a worldwide distribution and is one of the most significant pathogens of fledglings of caged birds such as macaws, conures, eclectus parrots, ring-necked parrots, lovebirds, and budgerigars. Polyomavirus is highly infectious, although many infections may be asymptomatic. Disease in adult birds is rare and may require a simultaneous infection with Psittacine Beak and Feather Disease Virus. Birds infected with APV can develop abdominal distention and a feather abnormality known as “French molt”. The disease causes a lack of down feathers on the back and abdomen, filoplumes on the head and neck, and subcutaneous hemorrhage, sometimes culminating in mortality. APV and PBFD (psittacine beak and feather disease) virus cause similar feather abnormalities and it is difficult to differentiate the viral etiology based on clinical presentations. However, it is important to differentiate them since treatments and countermeasures differ for the two diseases.

Contents TM

VetPCR APV PCR Pre-mixture DNase/RNase-free water APV PCR Positive control APV PCR Positive control Pre-mixture BrigTM Molecular Weight marker Mineral Oil DNA purification kit (see step 6.1)

AVIAN

VET0001F(48) or (96)

Kit 48

Kit 96

48 1 1 4 1 1 50

96 1 1 8 1 1 100

Characteristics Ready to use : only DNA template and D.W. are needed Easy and speed protocol Stable for 1 year at -20ºC Time-saving and cost-effective

Storage Condition Store at -20ºC Expected PCR product size : 500 bp M

VetPCRTM APV Detection Kit is the direct detection of Avian polyomavirus on the basis of a genetic database, so it can diagnose very fast and accurately. It can amplify only specific gene using the PCR (Polymerase Chain Reaction) method, and take only 3 hours for detection. Therefore, it is a very fast, accurate and reliable technique.

48 & 96 Test/k

Cod. Kit

500bp

Fig1. Electrophoresis of PCR product by VetPCRTM APV Detection Kit Lane M : BrigTM Molecular Weight Marker (Bioingentech Ltda.) Lane P : Positive control Lane 1~3 : APV Positive sample Lane N : Negative control

500 bp

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VetPCRTM ASP.FUMI Detection Kit

Product

VetPCR ASP.FUMI Detection Kit

DESCRIPTION The causal agent of avian aspergillosis is a filamentous fungal pathogen; Aspergillus fumigatus, although A. flavus, A. niger, A. nidulans, and A. terreus may cause the disease as well. Fungal infection occurs in acute and chronic forms. The acute form primarily occurs in young birds and newly imported birds and is the result of exposure to a large number of spores. The chronic form is more likely to occur in older birds that have been in captivity. Aspergillosis spores are widespread in the environment and many birds may carry them in their lungs and air sacks until immunosuppression or stress triggers clinical disease. Aspergillosis has been diagnosed in a variety of captive and free-living species of birds. The fungus is ubiquitous in the environment and flourishes in rotting vegetation and decaying organic material. In the acute form, anorexia, dyspnea, or sudden death may occur. White, mucoid exudation, marked congestion of the lungs and air sacs, and pneumonic nodules may be present. In the chronic form, dyspnea, voice change, lethargy, depression, emaciation, polydipsia, and polyuria may occur. Extensive involvement of the respiratory tract can be present before clinical signs are apparent. Ataxia and paralysis occur of the central nervous system is affected.

Cod. Kit

48 & 96 Test/kit VET0001F(48) or (96)

Contents TM

VetPCR ASP.FUMI PCR Pre-mixture DNase/RNase-free water ASP.FUMI PCR Positive control ASP.FUMI PCR Positive control Pre-mixture BrigTM Molecular Weight marker Mineral Oil DNA purification kit (see step 6.1)

Kit 48

Kit 96

48 1 1 4 1 1 50

96 1 1 8 1 1 100

Characteristics Ready to use : only DNA template and D.W. are needed Easy and speed protocol Stable for 1 year at -20ยบC Time-saving and cost-effective

Storage Condition Store at -20ยบC Expected PCR product size : 348 bp M

400bp

AVIAN

VetPCRTM ASP.FUMI Detection Kit is the direct detection of Aspergillus fumigatus on the basis of a genetic database, so it can diagnose very fast and accurately. It can amplify only specific gene using the PCR (Polymerase Chain Reaction) method, and take only 3 hours for detection. Therefore, it is a very fast, accurate and reliable technique.

348 bp

Fig1. Electrophoresis of PCR product by VetPCRTM ASP.FUMI Detection Kit Lane M : BrigTM Molecular Weight Marker (Bioingentech Ltda.) Lane N : Negative control Lane P : Positive control Lane 1~3 : ASP.FUMI Positive sample

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VetPCRTM CAV Detection Kit

Product

VetPCR CAV Detection Kit

DESCRIPTION The chicken anemia virus (CAV) virus is a 25 nm, nonenveloped, icosahedral virus with a single-stranded, circular DNA genome. The proposed classification of CAV is a new family of viruses, designated Circoviridae. The disease hasbeen described in most countries with a developed chicken industry.The primary importance of CAV in commercially produced chickens is due to theimmunosuppression it causes in young chickens. Immunosuppression can adversely affect production characteristics including decreased weight gain, increased unevenness, poor response to vaccination and increased condemnations due to secondary infection. CAV confirmation requires detection of virus antigens or DNA in the thymus or bone marrow. Virus isolation also can be used but is slow and expensive.

48 & 96 Test/k

Contents TM

VetPCR CAV PCR Pre-mixture DNase/RNase-free water CAV PCR Positive control CAV PCR Positive control Pre-mixture BrigTM Molecular Weight marker Mineral Oil DNA purification kit (see step 6.1)

Cod. Kit VET0001F(48) or (96)

Kit 48

Kit 96

48 1 1 4 1 1 50

96 1 1 8 1 1 100

Characteristics Ready to use : only DNA template and D.W. are needed Easy and speed protocol Stable for 1 year at -20ยบC Time-saving and cost-effective

Storage Condition Store at -20ยบC

VetPCRTM CAV Detection Kit is the direct detection of Chicken Anemia Virus on the basis of a genetic database, so it can diagnose very fast and accurately. It can amplify only specific gene using the PCR (Polymerase Chain Reaction) method, and take only 3 hours for detection. Therefore, it is a very fast, accurate and reliable technique.

Expected PCR product size : 405bp M

400bp

AVIAN

Fig1. Electrophoresis of PCR product by VetPCRTM CAV Detection Kit Lane M : BrigTM Molecular Weight Marker (Bioingentech Ltda.) Lane P : Positive control Lane 1~3 : CAV Positive sample Lane N : Negative control

405bp

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VetPCRTM CHLA.PSI Detection Kit

Product

VetPCR CHLA.PSI Detection Kit

DESCRIPTION The members of the order Chlamydiales are obligate intracellular bacteria with a 2-stage developmental cycle of replication. After infection, the bacteria reside within a cytoplasmic inclusion where they replicate by binary fission, then break out of the host cell to be disseminated. Chlamydophila psitacci has a worldwide distribution. Birds are natural hosts but transmission of C. psittaci to humans occurs by inhalation of contaminated dust or through contact with the excretions of infected animals. Infections occur worldwide and have been identified in at least 150 avian species. Clinical signs vary greatly in severity and depend on the species and age of the bird. Avian chlamydiosis can produce upper respiratory signs, conjunctivitis, gastrointestinal dysfunction and hepatitis. Current commonly used methods of C. psitacci diagnosis are culture, ELISA, inmunofluorescent stains and serological tests. Over the last 15 years, advances in the field of molecular biology have allowed for the development of extremely sensitive and specific nucleic acid detection methods. The efficiency of molecular diagnostic techniques generally exceeds that of other methodologies.

AVIAN

VetPCRTM CHLA.PSI Detection Kit is the direct detection of Chlamydophila psittaci on the basis of a genetic database, so it can diagnose very fast and accurately. It can amplify only specific gene using the PCR (Polymerase Chain Reaction) method, and take only 3 hours for detection. Therefore, it is a very fast, accurate and reliable technique.

48 & 96 Test/kit

Contents TM

VetPCR CHLA.PSI PCR Pre-mixture DNase/RNase-free water CHLA.PSI PCR Positive control CHLA.PSI PCR Positive control Pre-mixture BrigTM Molecular Weight marker Mineral Oil DNA purification kit (see step 6.1)

Cod. Kit VET0001F(48) or (96)

Kit 48

Kit 96

48 1 1 4 1 1 50

96 1 1 8 1 1 100

Characteristics Ready to use : only DNA template and D.W. are needed Easy and speed protocol Stable for 1 year at -20ยบC Time-saving and cost-effective

Storage Condition Store at -20ยบC Expected PCR product size : 271bp M

300bp

271bp

Fig1. Electrophoresis of PCR product by VetPCRTM CHLA.PSI Detection Kit Lane M : BrigTM Molecular Weight Marker (Bioingentech Ltda.) Lane N : Negative control Lane P : Positive control Lane 1~3 : CHLA.PSI Positive sample

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VetPCRTM CLOS.PER Detection Kit

Product

VetPCR CLOS.PER Detection Kit

DESCRIPTION Clostridial necrotic enteritis is a relatively common disease in avian species. Clostridium perfringens is a large Gram negative, spore-forming, anaerobic bacterium that causes necrotic enteritis and subclinical disease in chickens and turkeys. Infection in commercial poultry has been increasing at an alarming rate. Symptoms vary depending on the type of Clostridial infection. Disease is generally caused by type-C strains of C. perfringens producing toxin in the small intestines of birds, resulting in rapped loss of condition and weight loss, lethargic behavior, decreased appetite, and blood stained or undigested food. The toxin, and its effects may remain in the system for extended periods of time even after the original bacterial infection has been treated. Fecal-oral infection usually occurs in clostridial enteritis.

48 & 96 Test/k

Contents TM

VetPCR CLOS.PER PCR Pre-mixture DNase/RNase-free water CLOS.PER PCR Positive control CLOS.PER PCR Positive control Pre-mixture BrigTM Molecular Weight marker Mineral Oil DNA purification kit (see step 6.1)

Cod. Kit VET0001F(48) or (96)

Kit 48

Kit 96

48 1 1 4 1 1 50

96 1 1 8 1 1 100

Characteristics Ready to use : only DNA template and D.W. are needed Easy and speed protocol Stable for 1 year at -20ยบC Time-saving and cost-effective

Storage Condition Store at -20ยบC

VetPCRTM CLOS.PER Detection Kit is the direct detection of Clostridium perfringens on the basis of a genetic database, so it can diagnose very fast and accurately. It can amplify only specific gene using the PCR (Polymerase Chain Reaction) method, and take only 3 hours for detection. Therefore, it is a very fast, accurate and reliable technique.

Expected PCR product size : 645bp M

700bp

645bp

AVIAN

Fig1. Electrophoresis of PCR product by VetPCRTM CLOS.PER Detection Kit Lane M : BrigTM Molecular Weight Marker (Bioingentech Ltda.) Lane P : Positive control Lane 1~3 : CLOS.PER Positive sample Lane N : Negative control

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VetPCRTM FUSAR.spp Detection Kit

Product

VetPCR FUSAR.spp Detection Kit

DESCRIPTION Fusarium is a large genus of filamentous fungi widely distributed in soil and in association with plants. Most species are harmless saprobes and are relatively abundant members of the soil microbial community. Some species produce mycotoxins in cereal crops that can affect human and animal health if they enter the food chain. The main toxins produced by these Fusarium species are fumonisins and trichothecenes. Fusarium toxins cause severe lesions in animals that eat the contaminated feed. Other Fusarium mycotoxins cause immuno-supression in animals, as well as peak erosion and high mortality in broilers and weak eggs shells in layers. VetPCRTM FUSAR.spp Detection Kit is the direct detection of Fusarium spp. on the basis of a genetic database, so it can diagnose very fast and accurately. It can amplify only specific gene using the PCR (Polymerase Chain Reaction) method, and take only 3 hours for detection. Therefore, it is a very fast, accurate and reliable technique.

Cod. Kit

48 & 96 Test/kit VET0001F(48) or (96)

Contents TM

VetPCR FUSAR.spp PCR Pre-mixture DNase/RNase-free water FUSAR.spp PCR Positive control FUSAR.spp PCR Positive control Pre-mixture BrigTM Molecular Weight marker Mineral Oil DNA purification kit (see step 6.1)

Kit 48

Kit 96

48 1 1 4 1 1 50

96 1 1 8 1 1 100

Characteristics Ready to use : only DNA template and D.W. are needed Easy and speed protocol Stable for 1 year at -20ยบC Time-saving and cost-effective

Storage Condition Store at -20ยบC Expected PCR product size : 626 bp M

700bp

626 bp

AVIAN

Fig1. Electrophoresis of PCR product by VetPCRTM FUSAR.spp Detection Kit Lane M : BrigTM Molecular Weight Marker (Bioingentech Ltda.) Lane N : Negative control Lane P : Positive control Lane 1~4 : FUSAR.spp Positive sample

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VetPCRTM HIS.MEL Detection Kit

Product

VetPCR HIS.MEL Detection Kit

DESCRIPTION Histomonas meleagridis, a flagellated protozoan parasite, is the causative agent of histomonosis in turkeys, chickens, and occasionally other galliform birds. In turkeys, most infections are fatal; in other birds, mortality is less common. The organs primarily affected by the parasite are the caeca and the liver. Signs are apparent 7-12 days after infection and include listlessness, drooping wings, unkempt feathers, and yellow droppings. Young birds have a more acute disease and die within a few days after signs appear. Older birds may be sick for some time and become emaciated before death.

VetPCRTM HIS.MEL Detection Kit is the direct detection of Histomonas meleagridi on the basis of a genetic database, so it can diagnose very fast and accurately. It can amplify only specific gene using the PCR (Polymerase Chain Reaction) method, and take only 3 hours for detection. Therefore, it is a very fast, accurate and reliable technique.

48 & 96 Test/kit

Contents TM

VetPCR HIS.MEL PCR Pre-mixture DNase/RNase-free water HIS.MEL PCR Positive control HIS.MEL PCR Positive control Pre-mixture BrigTM Molecular Weight marker Mineral Oil DNA purification kit (see step 6.1)

Cod. Kit VET0001F(48) or (96)

Kit 48

Kit 96

48 1 1 4 1 1 50

96 1 1 8 1 1 100

Characteristics Ready to use : only DNA template and D.W. are needed Easy and speed protocol Stable for 1 year at -20ยบC Time-saving and cost-effective

Storage Condition Store at -20ยบC Expected PCR product size : 312 bp M

300bp

312 bp

AVIAN

Fig1. Electrophoresis of PCR product by VetPCRTM HIS.MEL Detection Kit Lane M : BrigTM Molecular Weight Marker (Bioingentech Ltda.) Lane N : Negative control Lane P : Positive control Lane 1~3 : HIS.MEL Positive sample

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VetPCRTM ILTV Detection Kit

Product

VetPCR ILTV Detection Kit

DESCRIPTION Infectious laryngotracheitis virus (ILTV) causes a serious, worldwideoccurring respiratory disease of chickens which affects growth and egg production and may lead to death of the animals. The acute phase of infection lasts between 1and 2 weeks and is often associated with clinical signs like gasping, coughing,expectoration of bloody mucus, and conjunctivitis. Subsequently, an asymptomatic latent infection of the central nervous system can be established.For prevention of disease chickens are immunized with attenuated live-virus vaccines that are suitable for mass application via eye drop, aerosol, or drinking water. Infectious laryngotracheitis is a contagious respiratory disease which is characterized by gasping, neck extension and conjunctivitis (inflammation of the membrane around the eye). ILTV, also designated as gallid herpes virus 1, has been classified as a member of the Alphaherpesvirinae subfamily of the Herpesviridae and represents the only species presently in the genus Infectious Laryngotracheitis-like Viruses.

Contents TM

VetPCR ILTV PCR Pre-mixture DNase/RNase-free water ILTV PCR Positive control ILTV PCR Positive control Pre-mixture BrigTM Molecular Weight marker Mineral Oil DNA purification kit (see step 6.1)

VET0001F(48) or (96)

Kit 48

Kit 96

48 1 1 4 1 1 50

96 1 1 8 1 1 100

Characteristics Ready to use : only DNA template and D.W. are needed Easy and speed protocol Stable for 1 year at -20ยบC Time-saving and cost-effective

Storage Condition Store at -20ยบC Expected PCR product size : 666 bp M

VetPCRTM ILTV Detection Kit is the direct detection of Infectious Laryngotracheitis Virus on the basis of a genetic database, so it can diagnose very fast and accurately. It can amplify only specific gene using the PCR (Polymerase Chain Reaction) method, and take only 3 hours for detection. Therefore, it is a very fast, accurate and reliable technique.

48 & 96 Test/kit

Cod. Kit

700bp

AVIAN

Fig1. Electrophoresis of PCR product by VetPCRTM ILTV Detection Kit Lane M : BrigTM Molecular Weight Marker (Bioingentech Ltda.) Lane P : Positive control Lane 1~3 : ILTV Positive sample Lane N : Negative control

666 bp

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VetPCRTM MDV Detection Kit

Product

VetPCR MDV Detection Kit

DESCRIPTION Marek´s disease is one of the most ubiquitous avian infections; it is identified in chicken flocks worldwide. Every flock, except for those maintained under strict pathogen-free conditions, may be presumed to be infected. Although clinical disease is not always apparent in infected flocks, a subclinical decrease in growth rate and egg production may be economically important. Typically, affected birds show only depression before death, but a transient paralysis syndrome has been associated with Marek´s disease; chickens become ataxic for periods of several days and then recover. This syndrome is rare in immunized birds.

VetPCRTM MDV Detection Kit is the direct detection of Marek´s Disease Virus on the basis of a genetic database, so it can diagnose very fast and accurately. It can amplify only specific gene using the PCR (Polymerase Chain Reaction) method, and take only 3 hours for detection. Therefore, it is a very fast, accurate and reliable technique.

48 & 96 Test/kit

Contents TM

VetPCR MDV PCR Pre-mixture DNase/RNase-free water MDV PCR Positive control MDV PCR Positive control Pre-mixture BrigTM Molecular Weight marker Mineral Oil DNA purification kit (see step 6.1)

Cod. Kit VET0001F(48) or (96)

Kit 48

Kit 96

48 1 1 4 1 1 50

96 1 1 8 1 1 100

Characteristics Ready to use : only DNA template and D.W. are needed Easy and speed protocol Stable for 1 year at -20ºC Time-saving and cost-effective

Storage Condition Store at -20ºC Expected PCR product size : 296 bp M

300bp

296 bp

AVIAN

Fig1. Electrophoresis of PCR product by VetPCRTM MDV Detection Kit Lane M : BrigTM Molecular Weight Marker (Bioingentech Ltda.) Lane N : Negative control Lane P : Positive control Lane 1~3 : MDV Positive sample

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VetPCRTM MYC.GALL Detection Kit

Product

VetPCR MYC.GALL Detection Kit

DESCRIPTION Several Mycoplasma spp. have been isolated from avian hosts; M. gallisepticum (MG), M. iowae, M. meleagridis and M. synoviae (MS) are the most important. Mycoplasmas are fastidious bacteria 0.3~0.8ฮผm in diameter; they lack a cell wall and require a rich growth medium containing serum. Each has distinctive epidemiological and pathological characteristics. MG infection is commonly designated as chronic respiratory disease in chickens and infectious sinusitis in turkeys. Infection may also occur in pheasants, chukar partridges, and peafowl. Infection in pigeons, quail, ducks, geese, and psittacine birds should be considered. MS was first recognized as an acute to chronic infection of chickens and turkeys that produced an exudative tendonitis and bursitis; it now occurs most frequently as a subclinical infection of the upper respiratory tract. MS infection is also a complication of airsacculitis in association with Newcastle disease or infectious bronchitis.

48 & 96 Test/kit VET0001F(48) or (96)

Contents

Kit 48

Kit 96

48 1 1 4 1 1 50

96 1 1 8 1 1 100

VetPCR MYC.GALL PCR Pre-mixture DNase/RNase-free water MYC.GALL PCR Positive control MYC.GALL PCR Positive control Pre-mixture BrigTM Molecular Weight marker Mineral Oil DNA purification kit (see step 6.1)

Characteristics Ready to use : only DNA template and D.W. are needed Easy and speed protocol Stable for 1 year at -20ยบC Time-saving and cost-effective

Storage Condition Store at -20ยบC Expected PCR product size : 379 bp M

VetPCRTM MYC.GALL Detection Kit is the direct detection of Mycoplasma gallisepticum on the basis of a genetic database, so it can diagnose very fast and accurately. It can amplify only specific gene using the PCR (Polymerase Chain Reaction) method, and take only 3 hours for detection. Therefore, it is a very fast, accurate and reliable technique.

Cod. Kit

400bp

379 bp

AVIAN

Fig1. Electrophoresis of PCR product by VetPCRTM MYC.GALL Detection Kit Lane M : BrigTM Molecular Weight Marker (Bioingentech Ltda.) Lane P : Positive control Lane 1~3 : MYC.GALL Positive sample Lane N : Negative control

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VetPCRTM PBFDV Detection Kit

Product

VetPCR PBFDV Detection Kit

DESCRIPTION Psitticine Beak and Feather Disease (PBFD) is a chronic disease characterized by feather dystrophy and loss, beak deformity and ultimately, death. It is caused by a non-enveloped icosahedral DNA virus (PBFDV) belonging to the family Circoviridae. The disease has been reported in Australia, North America, Europe and Asia. PBFD virus usually infects birds less than 3 years of age. The virus is spread from mother to egg or directly to chicks. Viral particles can be spread in feather dust carried by air currents, dried feces or even on the clothing of human handlers. Nest materials, feeding formula, feeding utensils, nets, bird carriers, food dishes and other fomites are easily contaminated with this virus. The first clinically detectable sign of PBFD is the appearance of necrotic, abnormally formed feathers. Many birds infected with PBFD die with in 6-12 months of onset of clinical signs. However, some birds have been known to survive 10-15 years and become chronic carriers. Death usually occurs from secondary bacterial, fungal, parasitic, chlamydial, or viral infections.

AVIAN

VetPCRTM PBFDV Detection Kit is the direct detection of Psitticine Beak and Feather Disease on the basis of a genetic database, so it can diagnose very fast and accurately. It can amplify only specific gene using the PCR (Polymerase Chain Reaction) method, and take only 3 hours for detection. Therefore, it is a very fast, accurate and reliable technique.

48 & 96 Test/kit

Contents TM

VetPCR PBFDV PCR Pre-mixture DNase/RNase-free water PBFDV PCR Positive control PBFDV PCR Positive control Pre-mixture BrigTM Molecular Weight marker Mineral Oil DNA purification kit (see step 6.1)

Cod. Kit VET0001F(48) or (96)

Kit 48

Kit 96

48 1 1 4 1 1 50

96 1 1 8 1 1 100

Characteristics Ready to use : only DNA template and D.W. are needed Easy and speed protocol Stable for 1 year at -20ยบC Time-saving and cost-effective

Storage Condition Store at -20ยบC Expected PCR product size : 395bp M

400bp

395bp

Fig1. Electrophoresis of PCR product by VetPCRTM PBFDV Detection Kit Lane M : BrigTM Molecular Weight Marker (Bioingentech Ltda.) Lane N : Negative control Lane P : Positive control Lane 1~3 : PBFDV Positive sample

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VetPCRTM PiCV Detection Kit

Product

VetPCR PiCV Detection Kit

DESCRIPTION Infections with pigeon circovirus (PiCV) occur in young racing pigeons and pigeons raised for meat production and have been reported worldwide. The viral genome of PiCV (also known as columbid circovirus or CoCV) has been currently classified as a tentative member of family Circoviridae and the genome of PiCV is similar to other members of the family: It is a circular, single-stranded DNA of approximately 1.76–2.04 kb in size, contained within a non-enveloped protein coat. The disease characterized by non-specific clinical signs affects young pigeons up to 6 months of age. Clinical symptoms of secondary illnesses due to viral, bacterial, parasitic, or mycotic infections can be observed depending on the health status of the flock and the zoohygienic conditions of the farm. Infected pigeons may develop symptoms such as lethargy, anorexia, weight loss, reduced race performance, respiratory distress and diarrhea. Typical pathological findings due to circoviral infection are atrophy of the bursa of Fabricius (BF) and thymus.

48 & 96 Test/kit VET0001F(48) or (96)

Contents TM

VetPCR PiCV PCR Pre-mixture DNase/RNase-free water PiCV PCR Positive control PiCV PCR Positive control Pre-mixture BrigTM Molecular Weight marker Mineral Oil DNA purification kit (see step 6.1)

Kit 48

Kit 96

48 1 1 4 1 1 50

96 1 1 8 1 1 100

Characteristics Ready to use : only DNA template and D.W. are needed Easy and speed protocol Stable for 1 year at -20ºC Time-saving and cost-effective

Storage Condition Store at -20ºC Expected PCR product size : 551 bp M

VetPCRTM PiCV Detection Kit is the direct detection of Pigeon Circovirus on the basis of a genetic database, so it can diagnose very fast and accurately. It can amplify only specific gene using the PCR (Polymerase Chain Reaction) method, and take only 3 hours for detection. Therefore, it is a very fast, accurate and reliable technique.

Cod. Kit

600bp

AVIAN

Fig1. Electrophoresis of PCR product by VetPCRTM PiCV Detection Kit Lane M : BrigTM Molecular Weight Marker (Bioingentech Ltda.) Lane P : Positive control Lane 1~3 : PiCV Positive sample Lane N : Negative control

551 bp

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VetPCRTM PLASM.spp Detection Kit

Product

VetPCR PLASM.spp Detection Kit

DESCRIPTION Hemosporidia are microscopic, intracellular parasitic protozoans found within the blood cells and tissues of their avian hosts. Three closely related genera, Plasmodium, Haemoproteus, and Leucocytozoon, are commonly found in wild birds. Infections in highly susceptible species and age classes may result in death. Hemosporidia are transmitted from infected to uninfected birds by a variety of biting flies that serve as vectors, including mosquitoes, black flies, ceratopogonid flies and louse flies. The avian hemosporidia are cosmopolitan parasites of birds, and they have been found in 68 percent of the more than 3,800 species of birds that have been examined. Birds with acute infections of Plasmodium, Haemoproteus, and Leucocytozoon, may exhibit similar signs in the field. These include emaciation, loss of appetite, listlessness, difficulty in breathing, and weakness and lameness in one or both legs. Survivors develop persistent, low-level infections in the blood and tissues that stimulate immunity to reinfection. These survivors do not exhibit any signs of disease, but they serve as reservoirs of infection, allowing the parasites to survive droughts and cold winter weather when vector populations have died off.

AVIAN

VetPCRTM PLASM.spp Detection Kit is the direct detection of Plasmodium spp. on the basis of a genetic database, so it can diagnose very fast and accurately. It can amplify only specific gene using the PCR (Polymerase Chain Reaction) method, and take only 3 hours for detection. Therefore, it is a very fast, accurate and reliable technique.

48 & 96 Test/kit

Contents

Cod. Kit VET0001F(48) or (96)

Kit 48

Kit 96

48 1 1 4 1 1 50

96 1 1 8 1 1 100

VetPCR PLASM.spp PCR Pre-mixture DNase/RNase-free water PLASM.spp PCR Positive control PLASM.spp PCR Positive control Pre-mixture BrigTM Molecular Weight marker Mineral Oil DNA purification kit (see step 6.1)

Characteristics Ready to use : only DNA template and D.W. are needed Easy and speed protocol Stable for 1 year at -20ยบC Time-saving and cost-effective

Storage Condition Store at -20ยบC Expected PCR product size : 328 bp M

400bp

328 bp

Fig1. Electrophoresis of PCR product by VetPCRTM PLASM.spp Detection Kit Lane M : BrigTM Molecular Weight Marker (Bioingentech Ltda.) Lane N : Negative control Lane P : Positive control Lane 1~4 : PLASM.spp Positive sample

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VetPCRTM PsHVs Detection Kit

Product

VetPCR PsHVs Detection Kit

DESCRIPTION Pacheco’s disease is caused by Psittacid herpesviruses (PsHVs) which are a heterogeneous group of viruses. PsHV is an enveloped double-stranded DNA virus belonging to the family alphaherpesvirus. Infection of psittacine birds with Pacheco’s disease virus can result in an acute fatal disease. Many parrot species, originating from multiple geographic regions, are susceptible to Pacheco’s disease. Although some infected birds may survive this fatal disease, they may become latently infected. Some infected birds may develop no symptoms whatsoever, and these birds become chronic carriers of the virus. Pacheco’s disease show nonspecific signs such as lethargy, anorexia, ruffled feathers, sinusitis and intermittent diarrhea. Any type of stress (eg breeding) can activate the virus and result in its being shed in large numbers in the feces. The incubation period is thought to be as short as a few days in some of the smaller parrots. VetPCRTM PsHVs Detection Kit is the direct detection of Psittacid Herpesviruses on the basis of a genetic database, so it can diagnose very fast and accurately. It can amplify only specific gene using the PCR (Polymerase Chain Reaction) method, and take only 3 hours for detection. Therefore, it is a very fast, accurate and reliable technique.

Cod. Kit

48 & 96 Test/kit VET0001F(48) or (96)

Contents

Kit 48

Kit 96

48 1 1 4 1 1 50

96 1 1 8 1 1 100

VetPCR PsHVs PCR Pre-mixture DNase/RNase-free water PsHVs PCR Positive control PsHVs PCR Positive control Pre-mixture BrigTM Molecular Weight marker Mineral Oil DNA purification kit (see step 6.1)

Characteristics Ready to use : only DNA template and D.W. are needed Easy and speed protocol Stable for 1 year at -20ºC Time-saving and cost-effective

Storage Condition Store at -20ºC Expected PCR product size : 342 bp M

400bp

342 bp

AVIAN

Fig1. Electrophoresis of PCR product by VetPCRTM PsHVs Detection Kit Lane M : BrigTM Molecular Weight Marker (Bioingentech Ltda.) Lane P : Positive control Lane 1~3 : PsHVs Positive sample Lane N : Negative control

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VetPCRTM TRICH.GAL Detection Kit

Product

VetPCR TRICH.GAL Detection Kit

DESCRIPTION Trichomonas, a parasitic flagellated protozoan, have three to five anterior flagella, an undulating membrane, and a recurrent flagellum attached to the edge of the undulating membrane. Trichomonas gallinae causes avian trichomoniasis. When present, it is usually found in the upper digestive tract of many species of doves and gallinaceous birds. Some strains may also produce liver and lung lesions. The parasite is transferred to young from the mother during feeding. Transmission between birds may also occur from contaminated feed and water. Infection by this trichomonad can be fatal. The presence of this organism in doves is a common source of infection of falcons and hawks feeding on them. In addition to showing signs of general illness, for example lethargy and fluffed-up plumage, affected birds may drool saliva, regurgitate food, have difficulty in swallowing or show laboured breathing. Finches are frequently seen to have matted wet plumage around the face and beak. In some cases, swelling of the neck may be visible from a distance. The disease may progress over several days or even weeks, consequently affected birds are often emaciated.

AVIAN

VetPCRTM TRICH.GAL Detection Kit is the direct detection of Trichomonas gallinae on the basis of a genetic database, so it can diagnose very fast and accurately. It can amplify only specific gene using the PCR (Polymerase Chain Reaction) method, and take only 3 hours for detection. Therefore, it is a very fast, accurate and reliable technique.

48 & 96 Test/kit

Contents TM

VetPCR TRICH.GAL PCR Pre-mixture DNase/RNase-free water TRICH.GAL PCR Positive control TRICH.GAL PCR Positive control Pre-mixture BrigTM Molecular Weight marker Mineral Oil DNA purification kit (see step 6.1)

Cod. Kit VET0001F(48) or (96)

Kit 48

Kit 96

48 1 1 4 1 1 50

96 1 1 8 1 1 100

Characteristics Ready to use : only DNA template and D.W. are needed Easy and speed protocol Stable for 1 year at -20ยบC Time-saving and cost-effective

Storage Condition Store at -20ยบC Expected PCR product size : 247 bp M

300bp

247 bp

Fig1. Electrophoresis of PCR product by VetPCRTM TRICH.GAL Detection Kit Lane M : BrigTM Molecular Weight Marker (Bioingentech Ltda.) Lane P : Positive control Lane 1~3 : TRICH.GAL Positive sample Lane N : Negative control

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VetPCRTM AIV Detection Kit DESCRIPTION Avian influenza is an infection caused by avian (bird) influenza (flu) viruses. These influenza virus es occur naturally among birds. Wild birds worldwide carry the viruses in their intestines, but usually do not get sick from them. However, avian influenza is very contagious among birds and can make some domesticated birds, including chickens, ducks, and turkeys, very sick and kill them. Infected birds shed influenza virus in their saliva, nasal secretions, and feces. Susceptible birds become infected when they have contact with contaminated secretions or excretions or with surfaces that are contaminated with secretions or excretions from infected birds. Domesticated birds may become infected with avian influenza virus through direct contact with infected waterfowl or other infected poultry, or through contact with surfaces (such as dirt or cages) or materials (such as water or feed) that have been contaminated with the virus. VetPCRTM AIV Detection Kit is the direct detection of Avian influenza virus on the basis of a genetic database, so it can diagnose very fast and accurately. It can amplify only specific gene using the PCR (Polymerase Chain Reaction) method, and take only 4 hours for detection. Therefore, it is a very fast, accurate and reliable technique.

Product

48 & 96 Test/kit

Contents VetPCRTM AIV RT-PCR Pre-mixture VetPCRTM AIV PCR Pre-mixture BrigRT-PCRTM solution BiotechTM Transcriptase solution DNase/RNase-free water AIV RT-PCR Positive control AIV RT-PCR Positive control Pre-mixture AIV PCR Positive control Pre-mixture BrigTM Molecular Weight marker Mineral Oil RNA extraction kit (see step 6.1) Characteristics

Kit 48 48 48 1 1 1 1 4 4 1 1 50

Cod. Kit VET0001F(48) or (96) Kit 96 96 96 1 1 1 1 8 8 1 2 100

Ready to use : only DNA template and D.W. are needed Easy and speed protocol Stable for 1 year at -20ยบC Time-saving and cost-effective

Storage Condition Store at -20ยบC Expected PCR product size : 304 bp M

300bp

AVIAN

Product

VetPCRTM AIV Detection Kit

304 bp

Fig1. Electrophoresis of PCR product by VetPCRTM AIV Detection Kit Lane M : BrigTM Molecular Weight Marker (Bioingentech Ltda.) Lane N : Negative control Lane P : Positive control Lane 1~3 : AIV Positive sample

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VetPCRTM AIV-H5 Detection Kit DESCRIPTION Avian influenza is an infection caused by avian (bird) influenza (flu) viruses. These influenza viruses occur naturally among birds. Wild birds worldwide carry the viruses in their intestines, but usually do not get sick from them. However, avian influenza is very contagious among birds and can make some domesticated birds, including chickens, ducks, and turkeys, very sick and kill them. Infected birds shed influenza virus in their saliva, nasal secretions, and feces. Susceptible birds become infected when they have contact with contaminated secretions or excretions or with surfaces that are contaminated with secretions or excretions from infected birds. Domesticated birds may become infected with avian influenza virus through direct contact with infected waterfowl or other infected poultry, or through contact with surfaces (such as dirt or cages) or materials (such as water or feed) that have been contaminated with the virus.

AVIAN

Avian Influenza Virus H5 • Potentially nine different subtypes • Can be highly pathogenic or low pathogenic • H5 infections have been documented among humans, sometimes causing severe illness and death VetPCRTM AIV-H5 Detection Kit is the direct detection of avian influenza virus serotype H5 on the basis of a genetic database, so it can diagnose very fast and accurately. It can amplify only specific gene using the PCR (Polymerase Chain Reaction) method, and take only 4 hours for detection. Therefore, it is a very fast, accurate and reliable technique.

Product VetPCRTM AIV-H5 Detection Kit

Product 48 & 96 Test/kit

Contents VetPCRTM AIV-H5 RT-PCR Pre-mixture VetPCRTM AIV-H5 PCR Pre-mixture BrigRT-PCRTM solution BiotechTM Transcriptase solution DNase/RNase-free water AIV-H5 RT-PCR Positive control AIV-H5 RT-PCR Positive control Pre-mixture AIV-H5 PCR Positive control Pre-mixture BrigTM Molecular Weight marker Mineral Oil RNA extraction kit (see step 6.1) Characteristics

Kit 48 48 48 1 1 1 1 4 4 1 1 50

Cod. Kit VET0001F(48) or (96) Kit 96 96 96 1 1 1 1 8 8 1 2 100

Ready to use : only DNA template and D.W. are needed Easy and speed protocol Stable for 1 year at -20ºC Time-saving and cost-effective

Storage Condition Store at -20ºC Expected PCR product size : 362 bp M P 1 2

400bp

362 bp

Fig1. Electrophoresis of PCR product by VetPCRTM AIV-H5 Detection Kit Lane M : BrigTM Molecular Weight Marker (Bioingentech Ltda.) Lane P : Positive control Lane 1~3 : AIV-H5 Positive sample Lane N : Negative control

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VetPCRTM AIV-H7 Detection Kit DESCRIPTION Avian influenza is an infection caused by avian (bird) influenza (flu) viruses. These influenza viruses occur naturally among birds. Wild birds worldwide carry the viruses in their intestines, but usually do not get sick from them. However, avian influenza is very contagious among birds and can make some domesticated birds, including chickens, ducks, and turkeys, very sick and kill them. Infected birds shed influenza virus in their saliva, nasal secretions, and feces. Susceptible birds become infected when they have contact with contaminated secretions or excretions or with surfaces that are contaminated with secretions or excretions from infected birds. Domesticated birds may become infected with avian influenza virus through direct contact with infected waterfowl or other infected poultry, or through contact with surfaces (such as dirt or cages) or materials (such as water or feed) that have been contaminated with the virus.

AVIAN

Avian Influenza Virus H7 • Potentially nine different subtypes. • Can be highly pathogenic or low pathogenic. • H7 infection in humans is rare, but can occur among persons who have direct contact with infected birds; symptoms may include conjunctivitis and/or upper respiratory symptoms. VetPCRTM AIV-H7 Detection Kit is the direct detection of Avian influenza virus serotype H7 on the basis of a genetic database, so it can diagnose very fast and accurately. It can amplify only specific gene using the PCR (Polymerase Chain Reaction) method, and take only 4 hours for detection. Therefore, it is a very fast, accurate and reliable technique.

Product

VetPCRTM AIV-H7 Detection Kit

48 & 96 Test/kit

Contents VetPCRTM AIV-H7 RT-PCR Pre-mixture VetPCRTM AIV-H7 PCR Pre-mixture BrigRT-PCRTM solution BiotechTM Transcriptase solution DNase/RNase-free water AIV-H7 RT-PCR Positive control AIV-H7 RT-PCR Positive control Pre-mixture AIV-H7 PCR Positive control Pre-mixture BrigTM Molecular Weight marker Mineral Oil RNA extraction kit (see step 6.1) Characteristics

Kit 48 48 48 1 1 1 1 4 4 1 1 50

Cod. Kit VET0001F(48) or (96) Kit 96 96 96 1 1 1 1 8 8 1 2 100

Ready to use : only DNA template and D.W. are needed Easy and speed protocol Stable for 1 year at -20ºC Time-saving and cost-effective

Storage Condition Store at -20ºC Expected PCR product size : 212 bp M

300bp

212 bp

Fig1. Electrophoresis of PCR product by VetPCRTM AIV-H7 Detection Kit Lane M : BrigTM Molecular Weight Marker (Bioingentech Ltda.) Lane N : Negative control Lane P : Positive control Lane 1~4: AIV-H7 Positive sample

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VetPCRTM AIV-H9 Detection Kit DESCRIPTION Avian influenza is an infection caused by avian(bird) influenza(flu) viruses. These influenza viruses occur naturally among birds. Wild birds worldwide carry the viruses in their intestines, but usually do not get sick from them. However, avian influenza is very contagious among birds and can make some domesticated birds, including chickens, ducks, and turkeys, very sick and kill them. Infected birds shed influenza virus in their saliva, nasal secretions, and feces. Susceptible birds become infected when they have contact with contaminated secretions or excretions or with surfaces that are contaminated with secretions or excretions from infected birds. Domesticated birds may become infected with avian influenza virus through direct contact with infected waterfowl or other infected poultry, or through contact with surfaces (such as dirt or cages) or materials (such as water or feed) that have been contaminated with the virus. Avian Influenza Virus H9 • Potentially nine different subtypes • Documented only in low pathogenic form • At least three H9 infections in humans have been confirmed

Product VetPCRTM AIV-H9 Detection Kit

Contents VetPCRTM AIV-H9 RT-PCR Pre-mixture VetPCRTM AIV-H9 PCR Pre-mixture BrigRT-PCRTM solution BiotechTM Transcriptase solution DNase/RNase-free water AIV-H9 RT-PCR Positive control AIV-H9 RT-PCR Positive control Pre-mixture AIV-H9 PCR Positive control Pre-mixture BrigTM Molecular Weight marker Mineral Oil RNA extraction kit (see step 6.1) Characteristics

AVIAN

Kit 48 48 48 1 1 1 1 4 4 1 1 50

Cod. Kit VET0001F(48) or (96) Kit 96 96 96 1 1 1 1 8 8 1 2 100

Ready to use : only DNA template and D.W. are needed Easy and speed protocol Stable for 1 year at -20ºC Time-saving and cost-effective

Storage Condition Store at -20ºC Expected PCR product size : 445 bp M N P 1

500bp

VetPCRTM AIV-H9 Detection Kit is the direct detection of avian influenza virus serotype H9 on the basis of a genetic database, so it can diagnose very fast and accurately. It can amplify only specific gene using the PCR (Polymerase Chain Reaction) method, and take only 4 hours for detection. Therefore, it is a very fast, accurate and reliable technique.

Product 48 & 96 Test/kit

445 bp

Fig1. Electrophoresis of PCR product by VetPCRTM AIV-H9 Detection Kit Lane M : BrigTM Molecular Weight Marker (Bioingentech Ltda.) Lane N : Negative control Lane P : Positive control Lane 1~3 : AIV-H9 Positive sample

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VetPCRTM ALV Detection Kit DESCRIPTION Lymphoid leukosis occurs naturally only in chickens. The infection is known to occur in virtually all chicken flocks except for some SPF flocks from which it has been eradicated. The frequency of infection has been reduced substantially in the primary breeding stocks of several commercial poultry breeding companies.In recent years this control program has expanded, and infection has become infrequent or absent in certain commercial flocks. Lymphoid leukosis is caused by certain members of the leukosis/ sarcoma group of avian retroviruses. Isolates that can induce lymphoid leukosis in chickens are commonly called avian leukosis viruses and belong to subgroups A, B, C, D, and J. Subgroups A and B are most prevalent in western countries. Subgroup J virus has been isolated in England from broiler breeder stocks experiencing myeloid neoplasms (myelocytoma). A sixth subgroup (E) designates non-oncogenic endogenous viruses produced by viral genes integrated into the host cell DNA. The subgroups have distinct antigenicities and cellular host ranges that are determined by viral envelope glycoproteins. VetPCRTM ALV Detection Kit is the direct detection of Avian leukosis virus on the basis of a genetic database, so it can diagnose very fast and accurately. It can amplify only specific gene using the PCR (Polymerase Chain Reaction) method, and take only 4 hours for detection. Therefore, it is a very fast, accurate and reliable technique.

Product

48 & 96 Test/kit

Contents VetPCRTM ALV RT-PCR Pre-mixture VetPCRTM ALV PCR Pre-mixture BrigRT-PCRTM solution BiotechTM Transcriptase solution DNase/RNase-free water ALV RT-PCR Positive control ALV RT-PCR Positive control Pre-mixture ALV PCR Positive control Pre-mixture BrigTM Molecular Weight marker Mineral Oil RNA extraction kit (see step 6.1) Characteristics

Kit 48 48 48 1 1 1 1 4 4 1 1 50

Cod. Kit VET0001F(48) or (96) Kit 96 96 96 1 1 1 1 8 8 1 2 100

Ready to use : only DNA template and D.W. are needed Easy and speed protocol Stable for 1 year at -20ยบC Time-saving and cost-effective

Storage Condition Store at -20ยบC Expected PCR product size : 396 bp M

400bp

AVIAN

Product

VetPCRTM ALV Detection Kit

396 bp

Fig1. Electrophoresis of PCR product by VetPCRTM ALV Detection Kit Lane M : BrigTM Molecular Weight Marker (Bioingentech Ltda.) Lane P : Positive control Lane 1~3: ALV Positive sample Lane N : Negative control

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VetPCRTM IBDV Detection Kit DESCRIPTION Infectious bursal disease (IBD) is an acute, highly contagious viral infection of young chickens that has lymphoid tissue as its primary target with a special predilection for the bursa of Fabricius. It was first recognized as a specific disease entity by Cosgrove in 1962 and was referred to as ยกavian nephrosis because of the extreme kidney damage found in birds that succumbed to infection. Since the first outbreaks occurred in the area of Gumboro, Delaware, Gumboro disease was a synonym for this disease and is still frequently used. The incubation period is very short, and clinical signs of the disease are seen within 2-3 days after exposure. One of the earliest signs of infection in a flock is the tendency for some birds to pick at their own vents. Soiled vent feathers, whitish or watery diarrhea, anorexia, depression, ruffled feathers, trembling, severe prostration, and finally, death. Affected birds became dehydrated, and in terminal stages of the disease, had a subnormal temperature.

AVIAN

VetPCRTM IBDV Detection Kit is the direct detection of Infectious bursal disease virus on the basis of a genetic database, so it can diagnose very fast and accurately. It can amplify only specific gene using the PCR (Polymerase Chain Reaction) method, and take only 4 hours for detection. Therefore, it is a very fast, accurate and reliable technique.

Product VetPCRTM IBDV Detection Kit

Product 48 & 96 Test/kit

Contents VetPCRTM IBDV RT-PCR Pre-mixture VetPCRTM IBDV PCR Pre-mixture BrigRT-PCRTM solution BiotechTM Transcriptase solution DNase/RNase-free water IBDV RT-PCR Positive control IBDV RT-PCR Positive control Pre-mixture IBDV PCR Positive control Pre-mixture BrigTM Molecular Weight marker Mineral Oil RNA extraction kit (see step 6.1) Characteristics

Kit 48 48 48 1 1 1 1 4 4 1 1 50

Cod. Kit VET0001F(48) or (96) Kit 96 96 96 1 1 1 1 8 8 1 2 100

Ready to use : only DNA template and D.W. are needed Easy and speed protocol Stable for 1 year at -20ยบC Time-saving and cost-effective

Storage Condition Store at -20ยบC Expected PCR product size : 434 bp M N P 1

500bp

434 bp

Fig1. Electrophoresis of PCR product by VetPCRTM IBDV Detection Kit Lane M : BrigTM Molecular Weight Marker (Bioingentech Ltda.) Lane N : Negative control Lane P : Positive control Lane 1~3 : IBDV Positive sample

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VetPCRTM IBV Detection Kit DESCRIPTION Infectious bronchitis (IB) is an acute and highly contagious respiratory disease of chickens. The disease is characterized by respiratory signs including gasping,coughing, sneezing, tracheal rales, and nasal discharge. In young chickens, severe respiratory distress may occur. In layers, respiratory distress, decrease in egg production, and loss of internal egg quality and egg shell quality are reported. Some strains of the virus cause severe kidney damage and may be associated with high mortality. Infectious bronchitis virus (IBV, an enveloped and single-stranded RNA virus) is a member of the Coronaviridae, which includes two genera, Coronavirus and Torovirus, IBV being in Group 3 of the Coronavirus genus. VetPCRTM IBV Detection Kit is the direct detection of Infectious bronchitis virus on the basis of a genetic database, so it can diagnose very fast and accurately. It can amplify only specific gene using the PCR (Polymerase Chain Reaction) method, and take only 4 hours for detection. Therefore, it is a very fast, accurate and reliable technique.

Product

48 & 96 Test/kit

Contents VetPCRTM IBV RT-PCR Pre-mixture VetPCRTM IBV PCR Pre-mixture BrigRT-PCRTM solution BiotechTM Transcriptase solution DNase/RNase-free water IBV RT-PCR Positive control IBV RT-PCR Positive control Pre-mixture IBV PCR Positive control Pre-mixture BrigTM Molecular Weight marker Mineral Oil RNA extraction kit (see step 6.1) Characteristics

Kit 48 48 48 1 1 1 1 4 4 1 1 50

Cod. Kit VET0001F(48) or (96) Kit 96 96 96 1 1 1 1 8 8 1 2 100

Ready to use : only DNA template and D.W. are needed Easy and speed protocol Stable for 1 year at -20ยบC Time-saving and cost-effective

Storage Condition Store at -20ยบC Expected PCR product size : 332bp M

400bp

AVIAN

Product

VetPCRTM IBV Detection Kit

332bp

Fig1. Electrophoresis of PCR product by VetPCRTM IBV Detection Kit Lane M : BrigTM Molecular Weight Marker (Bioingentech Ltda.) Lane P : Positive control Lane 1~3: IBV Positive sample Lane N : Negative control

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VetPCRTM NDV Detection Kit DESCRIPTION Newcastle disease is caused by an RNA virus, paramyxovirus-1 (PMV-1), that can be categorized into three groups: the velogenic strains, which are highly pathogenic and easily transmitted; the mesogenic strains, which show intermediate pathogenicity; and the lentogenic strains, which show low pathogenicity in chickens.The signs depend greatly on whether the virus is neurotropic or viscerotropic.The neurotropic viruses result in respiratory and nervous signs. The viscerotropic viruses, which predominate worldwide, cause respiratory signs with peracute disease, watery-greenish diarrhea, and swelling of the tissues of the head and neck. Nervous signs are also frequently seen in exotic bird species (parrots, etc) infected with viscerotropic viruses and in poultry species that survive beyond the acute phase of the disease. VetPCRTM NDV Detection Kit is the direct detection of Newcastle disease virus on the basis of a genetic database, so it can diagnose very fast and accurately. It can amplify only specific gene using the PCR (Polymerase Chain Reaction) method, and take only 4 hours for detection. Therefore, it is a very fast, accurate and reliable technique.

Product

48 & 96 Test/kit

Contents VetPCRTM NDV RT-PCR Pre-mixture VetPCRTM NDV PCR Pre-mixture BrigRT-PCRTM solution BiotechTM Transcriptase solution DNase/RNase-free water NDV RT-PCR Positive control NDV RT-PCR Positive control Pre-mixture NDV PCR Positive control Pre-mixture BrigTM Molecular Weight marker Mineral Oil RNA extraction kit (see step 6.1) Characteristics

Kit 48 48 48 1 1 1 1 4 4 1 1 50

Cod. Kit VET0001F(48) or (96) Kit 96 96 96 1 1 1 1 8 8 1 2 100

Ready to use : only DNA template and D.W. are needed Easy and speed protocol Stable for 1 year at -20ยบC Time-saving and cost-effective

Storage Condition Store at -20ยบC Expected PCR product size : 544 bp M N P 1

600bp

AVIAN

Product

VetPCRTM NDV Detection Kit

544 bp

Fig1. Electrophoresis of PCR product by VetPCRTM NDV Detection Kit Lane M : BrigTM Molecular Weight Marker (Bioingentech Ltda.) Lane N : Negative control Lane P : Positive control Lane 1~4 : NDV Positive sample

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VetPCRTM PMV Detection Kit DESCRIPTION Avian paramyxovirus (PMV) infections have been reported in chickens and turkeys in association with respiratory disease or decreases in egg production. There are 9 recognized serotypes of avian paramyxoviruses (PMV-1 to PMV-9). Newcastle disease virus (PMV-1) is the most important pathogen of this group for poultry, but PMV-2, -3, -6, and -7 are occasionally associated with disease in chickens and turkeys. PMV-2 has been isolated from wild birds, mainly passerines, and caged psittacine species. The method of transmission to chickens or turkeys is unclear. PMV-3 has been isolated from imported exotic and other bird species held in captivity. Psittacines appear to be the primary host, although PMV-3 will spread among passerines in captivity. Infections by PMV-2, -3, -6, and -7 in turkeys have produced mild to severe respiratory disease, drops in egg production, reduced hatchability and infertility of eggs, and increased numbers of white-shelled eggs. Infection with PMV-2 has produced mild respiratory disease in chickens, but PMV-2 infection is usually most severe in turkeys, especially breeders. Infection is more severe when accompanied by secondary pathogens.

AVIAN

VetPCRTM PMV Detection Kit is the direct detection of avian paramyxovirus on the basis of a genetic database, so it can diagnose very fast and accurately. It can amplify only specific gene using the PCR (Polymerase Chain Reaction) method, and take only 4 hours for detection. Therefore, it is a very fast, accurate and reliable technique.

Product

VetPCRTM PMV Detection Kit

48 & 96 Test/kit

Contents VetPCRTM PMV RT-PCR Pre-mixture VetPCRTM PMV PCR Pre-mixture BrigRT-PCRTM solution BiotechTM Transcriptase solution DNase/RNase-free water PMV RT-PCR Positive control PMV RT-PCR Positive control Pre-mixture PMV PCR Positive control Pre-mixture BrigTM Molecular Weight marker Mineral Oil RNA extraction kit (see step 6.1) Characteristics

Kit 48 48 48 1 1 1 1 4 4 1 1 50

Cod. Kit VET0001F(48) or (96) Kit 96 96 96 1 1 1 1 8 8 1 2 100

Ready to use : only DNA template and D.W. are needed Easy and speed protocol Stable for 1 year at -20ยบC Time-saving and cost-effective

Storage Condition Store at -20ยบC Expected PCR product size : 446 bp M

500bp

446 bp

Fig1. Electrophoresis of PCR product by VetPCRTM PMV Detection Kit Lane M : BrigTM Molecular Weight Marker (Bioingentech Ltda.) Lane N : Negative control Lane P : Positive control Lane 1~3: PMV Positive sample

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VetPCRTM WNV Detection Kit DESCRIPTION West Nile virus (WNV) belongs to the genus Flavivirus of the family Flaviviridae and is an arthropod-borne virus. It possesses a single-stranded plus-sense RNA genome of approximately 11,000 nucleotides. It circulates in natural transmission cycles involving primarily Culex species mosquitoes and birds; humans and other mammals are thought to be incidental hosts. Most corvids infected with WNV die within 3 weeks of infection. Clinical signs prior to death may include incoordinated walking, weakness, lethargy, tremors, and abnormal head posture. Although crows and blue jays account for between 50 and 90 percent of reported avian cases, WNV infection has been identified in over 170 other avian species across North America. Most people who are affected with WNV either have no symptoms or experience mild illness with flu-like symptoms. In some people, particularly the elderly, WNV can cause serious disease that affects the brain tissue. In extreme cases it can cause serious neurological damage and can be fatal.

AVIAN

VetPCRTM WNV Detection Kit is the direct detection of West Nile virus on the basis of a genetic database, so it can diagnose very fast and accurately. It can amplify only specific gene using the PCR (Polymerase Chain Reaction) method, and take only 4 hours for detection. Therefore, it is a very fast, accurate and reliable technique.

Product VetPCRTM WNV Detection Kit

Product 48 & 96 Test/kit

Contents VetPCRTM WNV RT-PCR Pre-mixture VetPCRTM WNV PCR Pre-mixture BrigRT-PCRTM solution BiotechTM Transcriptase solution DNase/RNase-free water WNV RT-PCR Positive control WNV RT-PCR Positive control Pre-mixture WNV PCR Positive control Pre-mixture BrigTM Molecular Weight marker Mineral Oil RNA extraction kit (see step 6.1) Characteristics

Kit 48 48 48 1 1 1 1 4 4 1 1 50

Cod. Kit VET0001F(48) or (96) Kit 96 96 96 1 1 1 1 8 8 1 2 100

Ready to use : only DNA template and D.W. are needed Easy and speed protocol Stable for 1 year at -20ยบC Time-saving and cost-effective

Storage Condition Store at -20ยบC Expected PCR product size : 273bp M P 1 2

300bp

273bp

Fig1. Electrophoresis of PCR product by VetPCRTM WNV Detection Kit Lane M : BrigTM Molecular Weight Marker (Bioingentech Ltda.) Lane P : Positive control Lane 1~3 : WNV Positive sample Lane N : Negative control

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VetPCRTM ANA.MARGI Detection Kit

Product

VetPCR ANA.MARGI Detection Kit

DESCRIPTION

BOVINE

Anaplasmosis is caused by a parasite called, Anaplasma marginale. It invades and multiplies in red blood cells. This parasite can be spread both mechanically and biologically. Mechanical transmission occurs by direct inoculation of cattle with blood-contaminated hypodermic needles, and surgical or dehorning instruments. Tabanids, including horseflies, may also mechanically transmit the disease on their mouthparts after taking a blood meal from an infected animal. Ticks are biological vectors of A. marginale and spread of the disease is most likely to occur through infected tick bites. The species of ticks important in spreading A. marginale are present in Alberta. Outbreaks of Anaplasmosis are usually seasonal and occur during or immediately after the vector season. As the disease progresses, infected red blood cells are destroyed causing anemia and occasionally death. Animals sick with Anaplasmosis may have a fever, and be off feed, depressed, dehydrated, show rapid or difficult breathing and have anemia. A few animals may also become excited or aggressive if enough oxygen doesn’t reach the brain. Cattle of any age can become infected but calves rarely become sick. Infected calves may become carriers of the parasite. On the other hand, up to 30 to 50 percent of cattle that become infected at three years of age or older may die if they develop clinical signs of the disease. VetPCRTM ANA.MARGI Detection Kit is the direct detection of Anaplasma marginale on the basis of a genetic database, so it can diagnose very fast and accurately. It can amplify only specific gene using the PCR (Polymerase Chain Reaction) method, and take only 3 hours for detection. Therefore, it is a very fast, accurate and reliable technique.

Cod. Kit

48 & 96 Test/kit VET0001F(48) or (96)

Contents

Kit 48

Kit 96

48 1 1 4 1 1 50

96 1 1 8 1 1 100

VetPCR ANA.MARGI PCR Pre-mixture DNase/RNase-free water ANA.MARGI PCR Positive control ANA.MARGI PCR Positive control Pre-mixture BrigTM Molecular Weight marker Mineral Oil DNA purification kit (see step 6.1)

Characteristics Ready to use : only DNA template and D.W. are needed Easy and speed protocol Stable for 1 year at -20ºC Time-saving and cost-effective

Storage Condition Store at -20ºC Expected PCR product size : 517 bp M

600bp

517 bp

Fig1. Electrophoresis of PCR product by VetPCRTM ANA.MARGI Detection Kit Lane M : BrigTM Molecular Weight Marker (Bioingentech Ltda.) Lane N : Negative control Lane P : Positive control Lane 1~3 : ANA.MARGI Positive sample

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VetPCRTM BAB.BIGE Detection Kit

Product

VetPCR BAB.BIGE Detection Kit

DESCRIPTION Bovine babesiosis is a major tick-borne disease of cattle caused by protozoan parasites (Babesia spp). In tropical areas the agents are Babesia bovis and Babesia bigemina. Cattle with advanced babesiosis have a low exercise tolerance and sometimes collapse and die while being driven to a yard for treatment. Older animals are more acutely affected (clinical babesiosis is rare in cattle younger than six months). Abortions may occur when pregnant animals are severely affected. Similar clinical signs develop during infection with both organisms. However, the course of the disease differs markedly. Infections are characterized by; fever, anorexia, depression, increased respiratory rate particularly following exertion, muscle tremor, reluctance to move, and haemoglobinuria, occasionally signs of cerebral derangement such as circling, head pressing, mania and convulsions. While these signs are seen very early in the course of B. bovis infections, they only develop in B. bigemina infections in the latter stages when parasitaemia is well advanced. Anaemia and jaundice develop steadily with B. bovis, but much more precipitously with B. bigemina. B. bigemina-affected animals may exhibit irritability and aggression but not the central nervous system signs.

Cod. Kit

48 & 96 Test/kit VET0001F(48) or (96)

Contents

Kit 48

Kit 96

48 1 1 4 1 1 50

96 1 1 8 1 1 100

VetPCR BAB.BIGE PCR Pre-mixture DNase/RNase-free water BAB.BIGE PCR Positive control BAB.BIGE PCR Positive control Pre-mixture BrigTM Molecular Weight marker Mineral Oil DNA purification kit (see step 6.1)

Characteristics Ready to use : only DNA template and D.W. are needed Easy and speed protocol Stable for 1 year at -20ยบC Time-saving and cost-effective

Storage Condition Store at -20ยบC Expected PCR product size : 453 bp M

500bp

BOVINE

VetPCRTM BAB.BIGE Detection Kit is the direct detection of Babesia bigemina on the basis of a genetic database, so it can diagnose very fast and accurately. It can amplify only specific gene using the PCR (Polymerase Chain Reaction) method, and take only 3 hours for detection. Therefore, it is a very fast, accurate, reliable technique.

453 bp

Fig1. Electrophoresis of PCR product by VetPCRTM BAB.BIGE Detection Kit Lane M : BrigTM Molecular Weight Marker (Bioingentech Ltda.) Lane 1~4 : BAB.BIGE Positive sample Lane P : Positive control Lane N : Negative control

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VetPCRTM BAB.BOVI Detection Kit

Product

VetPCR BAB.BOVI Detection Kit

DESCRIPTION

BOVINE

Bovine babesiosis is a major tick-borne disease of cattle caused by protozoan parasites (Babesia spp). In tropical areas the agents are Babesia bovis and Babesia bigemina. Cattle with advanced babesiosis have a low exercise tolerance and sometimes collapse and die while being driven to a yard for treatment. Older animals are more acutely affected (clinical babesiosis is rare in cattle younger than six months). Abortions may occur when pregnant animals are severely affected. Similar clinical signs develop during infection with both organisms. However, the course of the disease differs markedly. Infections are characterized by; fever, anorexia, depression, increased respiratory rate particularly following exertion, muscle tremor, reluctance to move, and haemoglobinuria, occasionally signs of cerebral derangement such as circling, head pressing, mania and convulsions. While these signs are seen very early in the course of B. bovis infections, they only develop in B. bigemina infections in the latter stages when parasitaemia is well advanced. Anaemia and jaundice develop steadily with B. bovis, but much more precipitously with B. bigemina. B. bigemina-affected animals may exhibit irritability and aggression but not the central nervous system signs.

VetPCRTM BAB.BOVI Detection Kit is the direct detection of Babesia bovis on the basis of a genetic database, so it can diagnose very fast and accurately. It can amplify only specific gene using the PCR (Polymerase Chain Reaction) method, and take only 3 hours for detection. Therefore, it is a very fast, accurate and reliable technique.

Cod. Kit

48 & 96 Test/kit VET0001F(48) or (96)

Contents TM

VetPCR BAB.BOVI PCR Pre-mixture DNase/RNase-free water BAB.BOVI PCR Positive control BAB.BOVI PCR Positive control Pre-mixture BrigTM Molecular Weight marker Mineral Oil DNA purification kit (see step 6.1)

Kit 48

Kit 96

48 1 1 4 1 1 50

96 1 1 8 1 1 100

Characteristics Ready to use : only DNA template and D.W. are needed Easy and speed protocol Stable for 1 year at -20ยบC Time-saving and cost-effective

Storage Condition Store at -20ยบC Expected PCR product size : 629 bp M

700bp

629 bp

Fig1. Electrophoresis of PCR product by VetPCRTM BAB.BOVI Detection Kit Lane M : BrigTM Molecular Weight Marker (Bioingentech Ltda.) Lane P : Positive control Lane 1~4 : BAB.BOVI Positive sample Lane N : Negative control

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VetPCRTM BHV-1 Detection Kit

Product

VetPCR BHV-1 Detection Kit

DESCRIPTION Bovine herpesvirus type 1 (BHV-1) a member of the subfamily Alphaherpesvirinae of the Herpesviridae family. Subtypes of BHV-1 have been described on the basis of restriction enzyme cleavage patterns of viral DNA, these types are referred to as BHV-1.1 (respiratory subtype), BHV-1.2 (respiratory and genital subtype). Infectious bovine rhinotracheitis/infectious pustular vulvovaginitis (IBR/ IPV) is a significant disease among domestic and wild bovine animals. Clinical signs of the infection include symptoms of inflammatory processes on both respiratory and genital organs, and abortion. Young calves can develop a systemic disease affecting visceral organs. BHV-1 is able to establish a latent infection in the trigeminal or sacral ganglia. Animals with a latent BHV-1 infection may serve as a source of infection for susceptible animals if and when the virus is reactivated. The infection is transmitted mainly through respiratory, ocular or genital secretions in a direct contact between animals. However, the infection can also spread through fresh or frozen semen from infected bulls. VetPCRTM BHV-1 Detection Kit is the direct detection of bovine herpesvirus type 1 on the basis of a genetic database, so it can diagnose very fast and accurately. It can amplify only specific gene using the PCR (Polymerase Chain Reaction) method, and take only 3 hours for detection. Therefore, it is a very fast, accurate, reliable technique.

Cod. Kit

48 & 96 Test/kit VET0001F(48) or (96)

Contents

Kit 48

Kit 96

48 1 1 4 1 1 50

96 1 1 8 1 1 100

VetPCR BHV-1 PCR Pre-mixture DNase/RNase-free water BHV-1 PCR Positive control BHV-1 PCR Positive control Pre-mixture BrigTM Molecular Weight marker Mineral Oil DNA purification kit (see step 6.1)

Characteristics Ready to use : only DNA template and D.W. are needed Easy and speed protocol Stable for 1 year at -20ยบC Time-saving and cost-effective

Storage Condition Store at -20ยบC Expected PCR product size : 637 bp M

700bp

637 bp

BOVINE

Fig1. Electrophoresis of PCR product by VetPCRTM BHV-1 Detection Kit Lane M : BrigTM Molecular Weight Marker (Bioingentech Ltda.) Lane N : Negative control Lane P : Positive control Lane 1~4 : BHV-1 Positive sample

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VetPCRTM BLV Detection Kit

Product

VetPCR BLV Detection Kit

DESCRIPTION

BOVINE

Bovine leukosis is caused by Bovine Leukemia Virus (BLV), an oncogenic retrovirus which belongs to the Retroviridae family. This disease is widely distributed and endemic in many cattle herds, dairy cows are more commonly affected with enzootic bovine leukosis than beef cattle. Most cattle infected with BLV do not exhibit clinical signs. BLV infection is life-long in cattle so demonstration of serum antibodies to BLV indicates persistent infection. Persistent (or fluctuating) lymphocytosis develops in approximately 30% of BLV-infected cattle. Lymphoma (lymphosarcoma) develops in approximately 3% of BLV-infected cattle but usually not until they are at least six years-of-age, this form of lymphoma is termed the “enzootic”, “endemic”, or “adult” form of bovine leukosis. Clinical signs reflect organ involvement peripheral lymph nodes can be visually or palpably enlarged. When retrobulbar lymph nodes are enlarged, proptosis or exophthalmos can result. Cardiac involvement usually includes the right atrium and can result in congestive heart failure with subcutaneous, ventral-dependent edema. Poor reproductive performance and palpable enlargement of the uterine wall or intra-pelvic lymph nodes are also indicators of malignant lymphoma. VetPCRTM BLV Detection Kit is the direct detection of bovine leukemia virus on the basis of a genetic database, so it can diagnose very fast and accurately. It can amplify only specific gene using the PCR (Polymerase Chain Reaction) method, and take only 3 hours for detection. Therefore, it is a very fast, accurate, reliable technique.

Cod. Kit

48 & 96 Test/kit VET0001F(48) or (96)

Contents

Kit 48

Kit 96

48 1 1 4 1 1 50

96 1 1 8 1 1 100

VetPCR BLV PCR Pre-mixture DNase/RNase-free water BLV PCR Positive control BLV PCR Positive control Pre-mixture BrigTM Molecular Weight marker Mineral Oil DNA purification kit (see step 6.1)

Characteristics Ready to use : only DNA template and D.W. are needed Easy and speed protocol Stable for 1 year at -20ºC Time-saving and cost-effective

Storage Condition Store at -20ºC Expected PCR product size : 655 bp M

700bp

Fig1. Electrophoresis of PCR product by VetPCRTM BLV Detection Kit Lane M : BrigTM Molecular Weight Marker (Bioingentech Ltda.) Lane 1~3 : BLV Positive sample Lane P : Positive control Lane N : Negative control

655 bp

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VetPCRTM BRU.AB Detection Kit

Product

VetPCR BRU.AB Detection Kit

DESCRIPTION Bovine Brucellosis, a disease caused by the Gram negative intracellular facultative bacteria Brucella abortus, is a widely distributed zoonosis throughout the world, affecting mainly cattle and causing sterility in males and abortion in pregnant females. Brucellosis is a disease characterized by abortion, a reduction in fertility and decreased milk production. In cattle, lesions of the female reproductive tract and of the placenta and foetus can lead to abortion with consequent severe economic loss. The foetus, placenta and uterine fluid contain large quantities of B. abortus bacteria which can infect other cattle coming into contact with an infected animal around the time of calving. The organism continues to be excreted in the milk; in the past humans were frequently infected through drinking unpasteurised milk. Breeding bulls which are infected, can transmit the disease to cows at the time of service by infected semen.VetPCRTM BRU.AB Detection Kit is the direct detection of Brucella abortus on the basis of a genetic database, so it can diagnose very fast and accurately. It can amplify only specific gene using the PCR (Polymerase Chain Reaction) method, and take only 3 hours for detection. Therefore, it is a very fast, accurate, reliable technique.

Cod. Kit

48 & 96 Test/kit VET0001F(48) or (96)

Contents TM

VetPCR BRU.AB PCR Pre-mixture DNase/RNase-free water BRU.AB PCR Positive control BRU.AB PCR Positive control Pre-mixture BrigTM Molecular Weight marker Mineral Oil DNA purification kit (see step 6.1)

Kit 48

Kit 96

48 1 1 4 1 1 50

96 1 1 8 1 1 100

Characteristics Ready to use : only DNA template and D.W. are needed Easy and speed protocol Stable for 1 year at -20ยบC Time-saving and cost-effective

Storage Condition Store at -20ยบC Expected PCR product size : 322 bp M

400bp

322 bp

BOVINE

Fig1. Electrophoresis of PCR product by VetPCRTM BRU.AB Detection Kit Lane M : BrigTM Molecular Weight Marker (Bioingentech Ltda.) Lane N : Negative control Lane P : Positive control Lane 1~4 : BRU.AB Positive sample

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VetPCRTM LEPT.spp Detection Kit

Product

VetPCR LEPT.spp Detection Kit

DESCRIPTION Leptospirosis is a bacterial disease, caused by various strains of the Leptospira species, a flexible, spiral-shaped, Gram-negative spirochete. Leptospira affects all domestic species including humans. Leptospirosis is spread through the urine of infected animals, which gets into water or soil and can survive there for weeks to months. Humans and animals are most likely to get leptospirosis from drinking water contaminated with the bacteria.Human disease may range from mild to severe. Signs include flu-like illness (fever, body aches, headache), weakness, vomiting, mental confusion, jaundice (orange/yellow skin color), and stiff neck. Severe cases can lead to damage of the liver, kidney or central nervous system (brain, spinal cord). In cattle it is usually associated with: Milk Drop Syndrome, obviously a very serious consequence for dairy herds but also of serious consequence for suckler herds. It also can cause abortions and birth of weak or still-born calves.VetPCRTM LEPT.spp Detection Kit is the direct detection of Leptospira spp. on the basis of a genetic database, so it can diagnose very fast and accurately. It can amplify only specific gene using the PCR (Polymerase Chain Reaction) method, and take only 3 hours for detection. Therefore, it is a very fast, accurate, reliable technique.

Cod. Kit

48 & 96 Test/kit VET0001F(48) or (96)

Contents TM

VetPCR LEPT.spp PCR Pre-mixture DNase/RNase-free water LEPT.spp PCR Positive control LEPT.spp PCR Positive control Pre-mixture BrigTM Molecular Weight marker Mineral Oil DNA purification kit (see step 6.1)

Kit 48

Kit 96

48 1 1 4 1 1 50

96 1 1 8 1 1 100

Characteristics Ready to use : only DNA template and D.W. are needed Easy and speed protocol Stable for 1 year at -20ยบC Time-saving and cost-effective

Storage Condition Store at -20ยบC Expected PCR product size : 348 bp M

400bp

348 bp

BOVINE

Fig1. Electrophoresis of PCR product by VetPCRTM LEPT.spp Detection Kit Lane M : BrigTM Molecular Weight Marker (Bioingentech Ltda.) Lane P : Positive control Lane 1~4 : LEPT.spp Positive sample Lane N : Negative control

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VetPCRTM LISTE.MON Detection Kit

Product

VetPCR LISTE.MON Detection Kit

DESCRIPTION Listeriosis usually results from infection by Listeria monocytogenes, a Gram positive rod in the family Listeriaceae. This organism is a facultative intracellular pathogen and cause animal and human disease. Listeriosis is usually a serious problem only in pregnant women, newborns, the elderly, and immunocompromised or debilitated hosts. Most human infections are caused by eating the bacteria in food, but the bacteria can also be spread by inhalation or direct contact. Most cases in the United States involve newborns. Women can become infected during pregnancy but usually show no signs of illness, however their fetus or newborn infant can die from the infection. The elderly or individuals with weak immune systems are also at greater risk for the disease. A skin infection form of the disease can occur in people who handle sick animals.In ruminants, listeriosis can cause encephalitis (inflammation of the brain), abortion or blood poisoning. Signs include depression, loss of appetite, fever, lack of coordination, salivation, facial paralysis, and circling. Disease is more common in younger animals (1 to 3 years old). Infection can also cause mastitis in cows.VetPCRTM LISTE.MON Detection Kit is the direct detection of Listeria monocytogenes on the basis of a genetic database, so it can diagnose very fast and accurately. It can amplify only specific gene using the PCR (Polymerase Chain Reaction) method, and take only 3 hours for detection. Therefore, it is a very fast, accurate, reliable technique.

Cod. Kit

48 & 96 Test/kit VET0001F(48) or (96)

Contents

Kit 48

Kit 96

48 1 1 4 1 1 50

96 1 1 8 1 1 100

VetPCR LISTE.MON PCR Pre-mixture DNase/RNase-free water LISTE.MON PCR Positive control LISTE.MON PCR Positive control Pre-mixture BrigTM Molecular Weight marker Mineral Oil DNA purification kit (see step 6.1)

Characteristics Ready to use : only DNA template and D.W. are needed Easy and speed protocol Stable for 1 year at -20ยบC Time-saving and cost-effective

Storage Condition Store at -20ยบC Expected PCR product size : 322 bp M

400bp

322 bp

BOVINE

Fig1. Electrophoresis of PCR product by VetPCRTM LISTE.MON Detection Kit Lane M : BrigTM Molecular Weight Marker (Bioingentech Ltda.) Lane 1~3 : LISTE.MON Positive sample Lane P : Positive control Lane N : Negative control

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VetPCRTM MYCO.B Detection Kit

Product

VetPCR MYCO.B Detection Kit

BOVINE

DESCRIPTION Mycobacterium bovis is a Gram-positive bacillus with zoonotic potencial, that primarily causes tuberculosis in cattle, but it may also infect and cause illness among other animals, including humans. In humans, M. bovis can cause a type of tuberculosis that may affect the chest, lungs and other parts of the body.The most common route of human infection is from drinking raw (unpasteurized) milk or eating dairy products made from raw milk. Less commonly, the bacteria can enter the body by breathing or through breaks in the skin. Infected humans may show no signs, develop infection in the chest and lungs, or have infection in other parts of the body such as the kidney, spine, and brain. The symptoms of chest and lung infection can include fever, chest pain, and a cough. Infected individuals often cough up blood. In animals, bovine tuberculosis is usually a slowly progressive and debilitating disease, but can occasionally have a quick onset and progress rapidly. Early stages of the infection often show no signs. As the disease progresses, weight loss, lack of appetite, weakness, and a low-grade fever are common. If the disease involves the lungs, animals will have a cough that is worse in the morning, during cold weather or activity, and they may have difficulty breathing. If the digestive tract is involved, animals may develop diarrhea that comes and goes or become constipated. VetPCRTM MICO.B Detection Kit is the direct detection of Mycobacterium bovis on the basis of a genetic database, so it can diagnose very fast and accurately. It can amplify only specific gene using the PCR (Polymerase Chain Reaction) method, and take only 3 hours for detection. Therefore, it is a very fast, accurate, reliable technique.

Cod. Kit

48 & 96 Test/kit VET0001F(48) or (96)

Contents TM

VetPCR MYCO.B PCR Pre-mixture DNase/RNase-free water MYCO.B PCR Positive control MYCO.B PCR Positive control Pre-mixture BrigTM Molecular Weight marker Mineral Oil DNA purification kit (see step 6.1)

Kit 48

Kit 96

48 1 1 4 1 1 50

96 1 1 8 1 1 100

Characteristics Ready to use : only DNA template and D.W. are needed Easy and speed protocol Stable for 1 year at -20ยบC Time-saving and cost-effective

Storage Condition Store at -20ยบC Expected PCR product size : 547 bp M

600bp

547 bp

Fig1. Electrophoresis of PCR product by VetPCRTM MYCO.B Detection Kit Lane M : BrigTM Molecular Weight Marker (Bioingentech Ltda.) Lane N : Negative control Lane 1~3 : MYCO.B Positive sample Lane P : Positive control

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VetPCRTM MRSA Detection Kit

Product

VetPCR MRSA Detection Kit

DESCRIPTION Methicillin resistant Staphylococcus aureus (MRSA), is a type of Staphylococcus aureus that is resistant to the antibacterial activity of methicillin and other related antibiotics of the penicillin class. Mastitis, which may be clinical (severe) or subclinical (moderate), is an important mammary gland disease that is usually caused by bacterial infection. If untreated, it constitutes a serious problem in dairy herds with considerable economic consequences, mainly due to reduced milk production and discarded milk.S. aureus is one of the most important etiologic agents in mastitis of cows, goats, and sheep. Moreover, S. aureus is probably the most infectious agent because it causes a chronic and deep infection in the mammary glands that is extremely difficult to cure.The prevalence of clinical and subclinical bovine mastitis caused by S. aureus ranges from 5 to 50% in different countries. VetPCRTM MRSA Detection Kit is the direct detection of Methicillin resistant Staphylococcus aureus on the basis of a genetic database, so it can diagnose very fast and accurately. It can amplify only specific gene using the PCR (Polymerase Chain Reaction) method, and take only 3 hours for detection. Therefore, it is a very fast, accurate and reliable technique.

Cod. Kit

48 & 96 Test/kit VET0001F(48) or (96)

Contents

Kit 48

Kit 96

48 1 1 4 1 1 50

96 1 1 8 1 1 100

VetPCR MRSA PCR Pre-mixture DNase/RNase-free water MRSA PCR Positive control MRSA PCR Positive control Pre-mixture BrigTM Molecular Weight marker Mineral Oil DNA purification kit (see step 6.1)

Characteristics Ready to use : only DNA template and D.W. are needed Easy and speed protocol Stable for 1 year at -20ยบC Time-saving and cost-effective

Storage Condition Store at -20ยบC Expected PCR product size : 261 bp M

300bp

261 bp

BOVINE

Fig1. Electrophoresis of PCR product by VetPCRTM MRSA Detection Kit Lane M : BrigTM Molecular Weight Marker (Bioingentech Ltda.) Lane P : Positive control Lane 1~4 : MRSA Positive sample Lane N : Negative control

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VetPCRTM SALMO.spp. Detection Kit

Product

VetPCR SALMO.spp. Detection Kit

DESCRIPTION Salmonella are facultative anaerobic, non-spore forming, gram-negative rods of the family Enterobacteriaceae. Enteric salmonellosis is characterized by an acute colitis that results in profuse diarrhea and, occasionally, abdominal pain. Animals become infected with Salmonella through a fecal-oral route of transmission. This occurs by eating materials (e.g., feed, water, pasture grass) contaminated with feces of an infected animal. The bacteria can live for months to years in warm, wet environments. Signs can include watery, foul smelling diarrhea, abdominal pain and fever. Dehydration and shock can occur and lead to death; this is most common in young animals. Some animals infected with Salmonella may not show signs of illness but shed the bacteria in their feces when they are stressed. VetPCRTM SALMOspp. Detection Kit is the direct detection of Salmonella spp. on the basis of a genetic database, so it can diagnose very fast and accurately. It can amplify only specific gene using the PCR (Polymerase Chain Reaction) method, and take only 3 hours for detection. Therefore, it is a very fast, accurate, reliable technique.

Cod. Kit

48 & 96 Test/kit VET0001F(48) or (96)

Contents

Kit 48

Kit 96

48 1 1 4 1 1 50

96 1 1 8 1 1 100

VetPCR SALMO.spp. PCR Pre-mixture DNase/RNase-free water SALMO.spp. PCR Positive control SALMO.spp. PCR Positive control Pre-mixture BrigTM Molecular Weight marker Mineral Oil DNA purification kit (see step 6.1)

Characteristics Ready to use : only DNA template and D.W. are needed Easy and speed protocol Stable for 1 year at -20ยบC Time-saving and cost-effective

Storage Condition Store at -20ยบC Expected PCR product size : 319 bp M

400bp

319 bp

BOVINE

Fig1. Electrophoresis of PCR product by VetPCRTM SALMO.spp. Detection Kit Lane M : BrigTM Molecular Weight Marker (Bioingentech Ltda.) Lane N : Negative control Lane P : Positive control Lane 1~4 : SALMO.spp. Positive sample

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VetPCRTM BCoV Detection Kit DESCRIPTION

BOVINE

Bovine coronavirus (BCoV) a member of the Coronaviridae family, possesses a single-stranded, enveloped, non-segmented RNA genome with positive polarity. BCoV is recognized as the one important pathogen in cattle worldwide, that causes three distinct clinical syndromes in bovine; calf diarrhea, winter dysentery in adult cattle, and as cause of respiratory tract infections in calves and feedlot cattle, including shipping fever. Calf diarrhea, characterized in young calves by profuse watery or hemorrhagic diarrhea, dehydration and often death; winter dysentery, that occurs primarily in adult dairy cows, and is recognized by severe watery diarrhea (sometimes with blood and mucus), decreased milk production, depression, anorexia and sometimes nasolagrimal discharge; and finally as cause of respiratory infections in cattle, including shipping fever. The diagnostic of BCoV by considering the clinical signs and anatomopathologic lesions is not possible, because other disease can appear similar to these syndromes. VetPCRTM BCoV Detection Kit is the direct detection of bovine coronavirus on the basis of a genetic database, so it can diagnose very fast and accurately. It can amplify only specific gene using the PCR (Polymerase Chain Reaction) method, and take only 3 hours for detection. Therefore, it is a very fast, accurate, reliable technique.

Product

VetPCRTM BCoV Detection Kit

48 & 96 Test/kit

Contents VetPCRTM BCoV RT-PCR Pre-mixture VetPCRTM BCoV PCR Pre-mixture BrigRT-PCRTM solution BiotechTM Transcriptase solution DNase/RNase-free water BCoV RT-PCR Positive control BCoV RT-PCR Positive control Pre-mixture BCoV PCR Positive control Pre-mixture BrigTM Molecular Weight marker Mineral Oil RNA extraction kit (see step 6.1) Characteristics

Kit 48 48 48 1 1 1 1 4 4 1 1 50

Cod. Kit VET0001F(48) or (96) Kit 96 96 96 1 1 1 1 8 8 1 2 100

Ready to use : only DNA template and D.W. are needed Easy and speed protocol Stable for 1 year at -20ยบC Time-saving and cost-effective

Storage Condition Store at -20ยบC Expected PCR product size : 692 bp M N P 1

700bp

692 bp

Fig1. Electrophoresis of PCR product by VetPCRTM BCoV Detection Kit Lane M : BrigTM Molecular Weight Marker (Bioingentech Ltda.) Lane N : Negative control Lane P : Positive control Lane 1~4 : BCoV Positive sample

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VetPCRTM BRV Detection Kit DESCRIPTION

BOVINE

Bovine rotavirus (BRV) a member of the Reoviridae family, is a major cause of diarrhea in young calves. It is nonenveloped and has double-shelled capsids surrounding a genome of 11 double-stranded RNA segments. Seven serological groups of rotavirus, A to G, have been identified, but only groups A, B, C, D, and G have been characterized well. Among the seven serogroups, group A rotavirus has been studied in greatest detail, and it is the serogroup most commonly found in cattle worldwide.BRV cause rapid onset of diarrhea in calves from 1 day of age up to about 3 weeks of age; most cases occur in the first week of life. The clinical signs mimic enteric colibacillosis: yellow watery diarrhea, varying degrees of dehydration, acidosis, and electrolyte derangements (hyponatremia, hypochloremia, hyperkalemia), inappetence, and reluctance to stand. Uncomplicated cases are self-limiting and symptomatic for only 1-2 days. However, secondary infections occur commonly, and will influence the ultimate clinical course of the illness. VetPCRTM BRV Detection Kit is the direct detection of bovine rotavirus on the basis of a genetic database, so it can diagnose very fast and accurately. It can amplify only specific gene using the PCR (Polymerase Chain Reaction) method, and take only 3 hours for detection. Therefore, it is a very fast, accurate, reliable technique.

Product

VetPCRTM BRV Detection Kit

48 & 96 Test/kit

Contents VetPCRTM BRV RT-PCR Pre-mixture VetPCRTM BRV PCR Pre-mixture BrigRT-PCRTM solution BiotechTM Transcriptase solution DNase/RNase-free water BRV RT-PCR Positive control BRV RT-PCR Positive control Pre-mixture BRV PCR Positive control Pre-mixture BrigTM Molecular Weight marker Mineral Oil RNA extraction kit (see step 6.1) Characteristics

Kit 48 48 48 1 1 1 1 4 4 1 1 50

Cod. Kit VET0001F(48) or (96) Kit 96 96 96 1 1 1 1 8 8 1 2 100

Ready to use : only DNA template and D.W. are needed Easy and speed protocol Stable for 1 year at -20ยบC Time-saving and cost-effective

Storage Condition Store at -20ยบC Expected PCR product size : 357 bp M

400bp

357 bp

Fig1. Electrophoresis of PCR product by VetPCRTM BRV Detection Kit Lane M : BrigTM Molecular Weight Marker (Bioingentech Ltda.) Lane N : Negative control Lane 1~4 : BRV Positive sample Lane P : Positive control

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VetPCRTM BVDV Detection Kit DESCRIPTION Most Bovine Viral Diarrhoea Virus (BVDV) (a member of the Flaviviridae family) infections are subclinical, but the clinical disease syndromes can be grouped into three categories: acute BVD, in utero infections, and diseases in persistently infected animals. Acute BVD can vary greatly in presentation from fever, depression, and runny nose and eyes, to diarrhea to respiratory disease, and can end in complete recovery or death depending on several factors: including, immune status of the animal, strain they are infected with, and age of the animal. In utero infections with BVDV can result in abortion, persistently infected animals, congenital defects, or normal, immuned calves depending on the stage of gestation the cow is in and her immune staยฌtus when she is infected with the virus. Persistently infected animals can result from in utero infection. The prevalence of these cattle are low (0.5 to 3 percent); but their potential to shed large quantities of virus and infect other animals in the herd is tremendous.

BOVINE

VetPCRTM BVDV Detection Kit is the direct detection of bovine viral diarrhoea virus on the basis of a genetic database, so it can diagnose very fast and accurately. It can amplify only specific gene using the PCR (Polymerase Chain Reaction) method, and take only 3 hours for detection. Therefore, it is a very fast, accurate, reliable technique.

Product

VetPCRTM BVDV Detection Kit

48 & 96 Test/kit

Contents VetPCRTM BVDV RT-PCR Pre-mixture VetPCRTM BVDV PCR Pre-mixture BrigRT-PCRTM solution BiotechTM Transcriptase solution DNase/RNase-free water BVDV RT-PCR Positive control BVDV RT-PCR Positive control Pre-mixture BVDV PCR Positive control Pre-mixture BrigTM Molecular Weight marker Mineral Oil RNA extraction kit (see step 6.1) Characteristics

Kit 48 48 48 1 1 1 1 4 4 1 1 50

Cod. Kit VET0001F(48) or (96) Kit 96 96 96 1 1 1 1 8 8 1 2 100

Ready to use : only DNA template and D.W. are needed Easy and speed protocol Stable for 1 year at -20ยบC Time-saving and cost-effective

Storage Condition Store at -20ยบC Expected PCR product size : 690 bp M

700bp

690 bp

Fig1. Electrophoresis of PCR product by VetPCRTM BVDV Detection Kit Lane M : BrigTM Molecular Weight Marker (Bioingentech Ltda.) Lane 1~3 : BVDV Positive sample Lane P : Positive control Lane N : Negative control

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VetPCRTM AN.PHA Detection Kit

Product

VetPCR AN.PHA Detection Kit

DESCRIPTION Anaplasma spp. are Gram-negative nonmotile, coccoid to ellipsoid bacteria that infect many animal species and cause various diseases. They are obligate intracellular parasites and are usually transmitted through the bite of infected arthropods such as Ixodes scapularis and Rhipicephalus sanguineus. Anaplasma phagocytophilum, formerly known as Ehrlichia phagocytophila, infects neutrophils, and rarely eosinophils, of dogs, cats, ruminants, as well as humans and causes granulocytotropic anaplasmosis. Common clinical signs of canine anaplasmosis include high fever, lethargy, depression and polyarthitis. Neurologic signs (ataxia, seizures and neck pain) can also be seen. VetPCRTM AN.PHA Detection Kit is the direct detection of Anaplasma phagocytophilum on the basis of a genetic database, so it can diagnose very fast and accurately. It can amplify only specific gene using the PCR (Polymerase Chain Reaction) method, and take only 3 hours for detection. Therefore, it is a very fast, accurate and reliable technique.

Cod. Kit

48 & 96 Test/kit VET0001F(48) or (96)

Contents

Kit 48

Kit 96

48 1 1 4 1 1 50

96 1 1 8 1 1 100

VetPCR AN.PHA PCR Pre-mixture DNase/RNase-free water AN.PHA PCR Positive control AN.PHA PCR Positive control Pre-mixture BrigTM Molecular Weight marker Mineral Oil DNA purification kit (see step 6.1)

Characteristics Ready to use : only DNA template and D.W. are needed Easy and speed protocol Stable for 1 year at -20ยบC Time-saving and cost-effective

Storage Condition Store at -20ยบC Expected PCR product size : 452 bp M

500bp

452 bp

CANINE

Fig1. Electrophoresis of PCR product by VetPCRTM AN.PHA Detection Kit Lane M : BrigTM Molecular Weight Marker (Bioingentech Ltda.) Lane 1~3 : AN.PHA Positive sample Lane P : Positive control Lane N : Negative control

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VetPCRTM AN.PTY Detection Kit

Product

VetPCR AN.PTY Detection Kit

DESCRIPTION Anaplasma platys (formerly Ehrlichia platys) is a small rickettsia that can cause ehrlichiosis in dogs. It is usually transmitted through the bite of an infected tick (Rhipicephalus sanguineus) or other arthropod. Infectious Canine Cyclic Thrombocytopenia (ICCT) was the name given to the disease caused by this strain. In most previous reports, ICCT was considered a benign disease without obvious clinical signs. Therefore, A. platys was not considered a very pathogenic organism or an important disease-causing agent. However, other investigators have reported more severe clinical signs, similar in severity to those associated with E. canis infections. Dogs infected with these rickettsiae will develop cyclic parasitemia and cyclic thrombocytopenia at 7- to 14-day intervals.

Cod. Kit

48 & 96 Test/kit VET0001F(48) or (96)

Contents TM

VetPCR AN.PTY PCR Pre-mixture DNase/RNase-free water AN.PTY PCR Positive control AN.PTY PCR Positive control Pre-mixture BrigTM Molecular Weight marker Mineral Oil DNA purification kit (see step 6.1)

Kit 48

Kit 96

48 1 1 4 1 1 50

96 1 1 8 1 1 100

Characteristics Ready to use : only DNA template and D.W. are needed Easy and speed protocol Stable for 1 year at -20ยบC Time-saving and cost-effective

Storage Condition Store at -20ยบC

VetPCRTM AN.PTY Detection Kit is the direct detection of Anaplasma platys on the basis of a genetic database, so it can diagnose very fast and accurately. It can amplify only specific gene using the PCR (Polymerase Chain Reaction) method, and take only 3 hours for detection. Therefore, it is a very fast, accurate and reliable technique.

Expected PCR product size : 432bp M

500bp

432bp

CANINE

Fig1. Electrophoresis of PCR product by VetPCRTM AN.PTY Detection Kit Lane M : BrigTM Molecular Weight Marker (Bioingentech Ltda.) Lane N : Negative control Lane 1~4 : AN.PTY Positive sample Lane P : Positive control

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VetPCRTM BAB.C Detection Kit

Product

VetPCR BAB.C Detection Kit

DESCRIPTION Babesia spp. are protozoal organisms that parasitize erythrocytes, causing anemia in the host. Many different species exist with varying host specificity. B. canis and B. gibsoni are two organisms commonly known to infect dogs. Both organisms have Ixodid tick vectors and are found throughout Asia, Africa, Europe, the Middle East, and North America, with B. canis being more prevalent. Cases of canine babesiosis may present with a wide variation of severity of clinical signs, ranging from a hyperacute, shock-associated, hemolytic crisis to an inapparent, subclinical infection. Dogs typically present with the acute form of babesiosis, which is characterized by general findings such as pyrexia, weakness, mucous membrane pallor, depression, lymphadenopathy, splenomegaly, and general malaise. Laboratory studies may document anemia, thrombocytopenia, hypoalbuminemia, and bilirubinuria. Initially, the anemia is normocytic, normochromic, and nonregenerative, but later develops into a macrocytic, hypochromic, regenerative anemia with reticulocytosis. The anemia is hypochromic because the reticulocytes have not yet formed their adult concentrations of hemoglobin.

Cod. Kit

48 & 96 Test/kit VET0001F(48) or (96)

Contents TM

VetPCR BAB.C PCR Pre-mixture DNase/RNase-free water BAB.C PCR Positive control BAB.C PCR Positive control Pre-mixture BrigTM Molecular Weight marker Mineral Oil DNA purification kit (see step 6.1)

Kit 48

Kit 96

48 1 1 4 1 1 50

96 1 1 8 1 1 100

Characteristics Ready to use : only DNA template and D.W. are needed Easy and speed protocol Stable for 1 year at -20ยบC Time-saving and cost-effective

Storage Condition Store at -20ยบC Expected PCR product size : 294 bp M

300bp

294 bp

CANINE

Fig1. Electrophoresis of PCR product by VetPCRTM BAB.C Detection Kit Lane M : BrigTM Molecular Weight Marker (Bioingentech Ltda.) Lane P : Positive control Lane 1~3 : BAB.C Positive sample Lane N : Negative control

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VetPCRTM BAR.spp Detection Kit

Product

VetPCR BAR.spp Detection Kit

DESCRIPTION Bartonella spp. are small Gram-negative rods, aerobic and highly fastidious bacteria. They are considered as emergent human pathogens and are the etiological agents for human Cat Scratch Disease (CSD). Recent studies have shown that pet cats serve as a major persistent reservoir host, with prolonged asymptomatic bacteremia, for five Bartonella species: B. henselae, B. clarridgeiae, B. koehlerae, B. weissii, and B. elizabethae. Cat and dog fleas and, less often, deer and dog ticks carry the bacteria and serve as the main vectors for transmission from cat to cat, and they also have the potential to act as vectors in transmitting Bartonella spp. from cats to humans. Bartonella spp. may also cause illness in dogs. Furthermore, infection with these bacteria appears to induce disease manifestations, such as endocarditis, peliosis hepatis, and granulomatous disease, that are similar to those observed in both dogs and humans. VetPCRTM BAR spp. Detection Kit is the direct detection of Batonella spp. on the basis of a genetic data base, so it can diagnose very fast and accurately. It can amplify only specific gene using the PCR (Polymerase Chain Reaction) method, and take only 3 hours for detection. Therefore, it is a very fast, accurate and reliable technique.

Cod. Kit

48 & 96 Test/kit VET0001F(48) or (96)

Contents

Kit 48

Kit 96

48 1 1 4 1 1 50

96 1 1 8 1 1 100

VetPCR BAR.spp PCR Pre-mixture DNase/RNase-free water BAR.spp PCR Positive control BAR.spp PCR Positive control Pre-mixture BrigTM Molecular Weight marker Mineral Oil DNA purification kit (see step 6.1)

Characteristics Ready to use : only DNA template and D.W. are needed Easy and speed protocol Stable for 1 year at -20ยบC Time-saving and cost-effective

Storage Condition Store at -20ยบC Expected PCR product size : 314 bp M

400bp

314 bp

CANINE

Fig1. Electrophoresis of PCR product by VetPCRTM BAR.spp Detection Kit Lane M : BrigTM Molecular Weight Marker (Bioingentech Ltda.) Lane N : Negative control Lane P : Positive control Lane 1~4 : BAR.spp Positive sample

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VetPCRTM BRU.CA Detection Kit

Product

VetPCR BRU.CA Detection Kit

DESCRIPTION

CANINE

Canine brucellosis is a disease caused by the bacteria Brucella canis, a Gram-negative coccobacillus or short rod. It is found throughout the world. It is spread through contact with aborted fetuses and discharges from the uterus of infected bitches, during mating, through maternal milk and possibly through airborne transmission in some cases. The bacteria enters the body through mucous membranes and spreads from there to lymph nodes and the spleen. It also spreads to the uterus, placenta and prostate gland as well as other internal organs at times. In female dogs, infection leads to abortion or early death of infected puppies. Infected females may have no other clinical signs. In some cases there may be decreased fertility rather than abortion. This may be due to resorption of fetuses early in their development. In male dogs, infection of the testicles can lead to infertility due to anti-sperm antibodies developed as the body attempts to fight off the bacterial infection. The testes may atrophy after the initial period of swelling. Scrotal enlargement or infection of the skin over the scrotum may be seen. In both female and male dogs there may be infection of spinal discs (diskospondylitis) which can cause back pain and rear leg weakness or even paralysis. Eye inflammation may be seen in either sex. VetPCRTM BRU.CA Detection Kit is the direct detection of Brucella canis on the basis of a genetic data base, so it can diagnose very fast and accurately. It can amplify only specific gene using the PCR (Polymerase Chain Reaction) method, and take only 3 hours for detection. Therefore, it is a very fast, accurate and reliable technique.

Cod. Kit

48 & 96 Test/kit VET0001F(48) or (96)

Contents TM

VetPCR BRU.CA PCR Pre-mixture DNase/RNase-free water BRU.CA PCR Positive control BRU.CA PCR Positive control Pre-mixture BrigTM Molecular Weight marker Mineral Oil DNA purification kit (see step 6.1)

Kit 48

Kit 96

48 1 1 4 1 1 50

96 1 1 8 1 1 100

Characteristics Ready to use : only DNA template and D.W. are needed Easy and speed protocol Stable for 1 year at -20ยบC Time-saving and cost-effective

Storage Condition Store at -20ยบC Expected PCR product size : 322 bp M

400bp

322 bp

Fig1. Electrophoresis of PCR product by VetPCRTM BRU.CA Detection Kit Lane M : BrigTM Molecular Weight Marker (Bioingentech Ltda.) Lane 1~3 : BRU.CA Positive sample Lane P : Positive control Lane N : Negative control

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VetPCRTM CAdV Detection Kit

Product

VetPCR CAdV Detection Kit

DESCRIPTION Canine hepatitis is a disease of the liver and other body organs caused by canine adenovirus type-1 (CAV-1) and canine adenovirus type-2 (CAV-2) which causes respiratory and enteric diseases. The virus is found worldwide and is spread by body fluids including nasal discharge and urine. Recovered patients can shed the virus for up to nine months in the urine. The primary mode of transmission is by direct contact with an infected dog. Contaminated runs, cages, dishes, hands, boots, etc., can also serve as a source of transmission. Initially, the virus affects the tonsils and larynx causing a sore throat, coughing, and occasionally pneumonia. As it enters the bloodstream, it can affect the eyes, liver, and kidneys. The clear portion of the eyes, called the cornea, may appear cloudy or bluish. This is due to edema within the cell layers forming the cornea. The name ‘hepatitis blue eye’ has been used to describe eyes so affected. As the liver and kidneys fail, one may notice seizures, increased thirst, vomiting, and/or diarrhea.

48 & 96 Test/kit VET0001F(48) or (96)

Contents TM

VetPCR CAdV PCR Pre-mixture DNase/RNase-free water CAdV PCR Positive control CAdV PCR Positive control Pre-mixture BrigTM Molecular Weight marker Mineral Oil DNA purification kit (see step 6.1)

Kit 48

Kit 96

48 1 1 4 1 1 50

96 1 1 8 1 1 100

Characteristics Ready to use : only DNA template and D.W. are needed Easy and speed protocol Stable for 1 year at -20ºC Time-saving and cost-effective

Storage Condition Store at -20ºC Expected PCR product size : 658 bp M

VetPCRTM CAdV Detection Kit is the direct detection of Canine adenovirus type-1/type-2 on the basis of a genetic database, so it can diagnose very fast and accurately. It can amplify only specific gene using the PCR (Polymerase Chain Reaction) method, and take only 3 hours for detection. Therefore, it is a very fast, accurate and reliable technique.

Cod. Kit

700bp

658 bp

CANINE

Fig1. Electrophoresis of PCR product by VetPCRTM CAdV Detection Kit Lane M : BrigTM Molecular Weight Marker (Bioingentech Ltda.) Lane 1~3 : CAdV Positive sample Lane P : Positive control Lane N : Negative control

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VetPCRTM CAMP.JE Detection Kit

Product

VetPCR CAMP.JE Detection Kit

DESCRIPTION Campylobacter sp. cause disease (usually diarrhea) in a variety of species including dogs, cats, birds, ferrets, guinea pigs hamsters, rabbits, rodents and other species - including humans. Organisms of the genus Campylobacter are Gram-negative, microaerophiles that cause 5-11% of all diarrhea cases. Many infected dogs show no signs. Stress, the presence of another disease or other factors (e.g. pregnancy) may increase susceptibility to develop the disease. Diarrhea (watery to bloody with mucus and sometimes bile-stained) lasting 5-15 days is usually how the disease presents, and it is most common in dogs less than 6 months old.Occasionally chronic diarrhea can result - lasting for months, and sometimes there is an increased body temperature and an increased white cell (leukocyte) count. Many infected cats show no signs. Usually affected cats are less than 6 months old. Diarrhea (sometimes bloody) is the sign seen most often, but in most of the cases reported in the literature other infectious agents were found to be present as well e.g. toxoplasma, giardia.

CANINE

VetPCRTM CAMP.JE Detection Kit is the direct detection of Campylobacter jejuni on the basis of a genetic database, so it can diagnose very fast and accurately. It can amplify only specific gene using the PCR (Polymerase Chain Reaction) method, and take only 3 hours for detection. Therefore, it is a very fast, accurate and reliable technique.

Cod. Kit

48 & 96 Test/kit VET0001F(48) or (96)

Contents

Kit 48

Kit 96

48 1 1 4 1 1 50

96 1 1 8 1 1 100

VetPCR CAMP.JE PCR Pre-mixture DNase/RNase-free water CAMP.JE PCR Positive control CAMP.JE PCR Positive control Pre-mixture BrigTM Molecular Weight marker Mineral Oil DNA purification kit (see step 6.1)

Characteristics Ready to use : only DNA template and D.W. are needed Easy and speed protocol Stable for 1 year at -20ยบC Time-saving and cost-effective

Storage Condition Store at -20ยบC Expected PCR product size : 304 bp M

300bp

304 bp

Fig1. Electrophoresis of PCR product by VetPCRTM CAMP.JE Detection Kit Lane M : BrigTM Molecular Weight Marker (Bioingentech Ltda.) Lane P : Positive control Lane 1~4 : CAMP.JE Positive sample Lane N : Negative control

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VetPCRTM CAdV-2 Detection Kit

Product

VetPCR CAdV-2 Detection Kit

DESCRIPTION Adenoviruses are linear, double-stranded DNA viruses which infect a wide variety of mammals and birds. Two adenoviruses have been identified in the dog: canine adenovirus type 1 (CAV-1) which infects most of the major organs causing, among other diseases, hepatitis; and canine adenovirus type 2 (CAV-2) which causes respiratory and enteric diseases. CAV-2 is one of the major causes of infectious tracheobronchitis in dogs, also known as kennel cough. Vaccination against CAV-2 will not prevent infection with this virus but it can minimize its severity so that the chances of developing secondary bacterial infections and complications are lessened. Normally, symptoms of kennel cough will develop within a week after a dog has been exposed to CAV-2. The most common symptoms are a dry, hacking cough followed by retching, and coughing up a white foamy discharge. The cough is brought on by an inflammation of the trachea and bronchi. Some dogs also develop conjunctivitis, rhinitis or a nasal discharge.

CANINE

VetPCRTM CAV-2 Detection Kit is the direct detection of Canine adenovirus type-2 on the basis of a genetic data base, so it can diagnose very fast and accurately. It can amplify only specific gene using the PCR (Polymerase Chain Reaction) method, and take only 3 hours for detection. Therefore, it is a very fast, accurate and reliable technique.

Cod. Kit

48 & 96 Test/kit VET0001F(48) or (96)

Contents TM

VetPCR CAdV-2 PCR Pre-mixture DNase/RNase-free water CAdV-2 PCR Positive control CAdV-2 PCR Positive control Pre-mixture BrigTM Molecular Weight marker Mineral Oil DNA purification kit (see step 6.1)

Kit 48

Kit 96

48 1 1 4 1 1 50

96 1 1 8 1 1 100

Characteristics Ready to use : only DNA template and D.W. are needed Easy and speed protocol Stable for 1 year at -20ยบC Time-saving and cost-effective

Storage Condition Store at -20ยบC Expected PCR product size : 658 bp M

700bp

658 bp

Fig1. Electrophoresis of PCR product by VetPCRTM CAdV-2 Detection Kit Lane M : BrigTM Molecular Weight Marker (Bioingentech Ltda.) Lane P : Positive control Lane 1~4 : CAdV-2 Positive sample Lane N : Negative control

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VetPCRTM CHLA.spp Detection Kit

Product

VetPCR CHLA.spp Detection Kit

DESCRIPTION Chlamydia spp. are a group of obligately intracellular bacteria that replicate inside of a cytoplasmic inclusion. They form spore-like structures that are called elementary bodies and are released from host cells and infect other cells. Chlamydiae are found in virtually all vertebrate and invertebrate animals. Currently nine pathogenic Chlamydia spp. are known that infect mammals. Chlamydial diseases are typically not caused by toxins produced by the bacteria but by an ineffective cellular immune response that fails to eliminate the bacteria. These diseases are typically chronic in nature, and manifest themselves as granulomatous lesions on mucosal membranes. Scarring lesions may be the consequence of repeated infections. Specific Chlamydia spp. are typically associated with specific hosts and disease manifestations. Acute chlamydial diseases may present as respiratory diseases (C. pneumoniae, C. psittaci, C. pecorum, C. suis), enteritis, polyarthritis (C. pecorum), or urogenital infections (C. trachomatis, C. suis, C. pecorum).

CANINE

VetPCRTM CHLA spp. Detection Kit is the direct detection of Chlamydia spp. on the basis of a genetic data base, so it can diagnose very fast and accurately. It can amplify only specific gene using the PCR (Polymerase Chain Reaction) method, and take only 3 hours for detection. Therefore, it is a very fast, accurate and reliable technique.

Cod. Kit

48 & 96 Test/kit VET0001F(48) or (96)

Contents TM

VetPCR CHLA.spp PCR Pre-mixture DNase/RNase-free water CHLA.spp PCR Positive control CHLA.spp PCR Positive control Pre-mixture BrigTM Molecular Weight marker Mineral Oil DNA purification kit (see step 6.1)

Kit 48

Kit 96

48 1 1 4 1 1 50

96 1 1 8 1 1 100

Characteristics Ready to use : only DNA template and D.W. are needed Easy and speed protocol Stable for 1 year at -20ยบC Time-saving and cost-effective

Storage Condition Store at -20ยบC Expected PCR product size : 261 bp M

300bp

261 bp

Fig1. Electrophoresis of PCR product by VetPCRTM CHLA.spp Detection Kit Lane M : BrigTM Molecular Weight Marker (Bioingentech Ltda.) Lane 1~3 : CHLA.spp Positive sample Lane P : Positive control Lane N : Negative control

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VetPCRTM CHV Detection Kit

Product

VetPCR CHV Detection Kit

DESCRIPTION Canine herpesvirus (CHV) can cause fading puppy syndrome, upper respiratory tract disease (kennel cough) and abortion/stillbirths in dogs. The main route of transmission appears to be oronasal from infected puppies or from nasal or vaginal excretions of adults. The virus spreads rapidly through kennels but usually only causes disease in very young puppies. Infection of newborn puppies commonly results in death. Puppies infected with CHV at the time of birth will generally start to show clinical signs of infection at four to six days of age. Infected puppies will exhibit persistent cry, a diminished suckling response, yellow green diarrhea and abdominal pain. Fever is usually not present. Death frequently occurs within 48 hours after clinical signs are noted. One or all pups in a litter infected at birth may show signs of herpesvirus infection. Infection of adults or puppies over 3 weeks old results in replication in the respiratory tract without clinical disease. The virus can undergo latent infection and reactivation, and further shedding can be induced by immunosuppression or stress.

CANINE

VetPCRTM CHV Detection Kit is the direct detection of Canine herpesvirus on the basis of a genetic database, so it can diagnose very fast and accurately. It can amplify only specific gene using the PCR (Polymerase Chain Reaction) method, and take only 3 hours for detection. Therefore, it is a very fast, accurate and reliable technique.

Cod. Kit

48 & 96 Test/kit VET0001F(48) or (96)

Contents TM

VetPCR CHV PCR Pre-mixture DNase/RNase-free water CHV PCR Positive control CHV PCR Positive control Pre-mixture BrigTM Molecular Weight marker Mineral Oil DNA purification kit (see step 6.1)

Kit 48

Kit 96

48 1 1 4 1 1 50

96 1 1 8 1 1 100

Characteristics Ready to use : only DNA template and D.W. are needed Easy and speed protocol Stable for 1 year at -20ยบC Time-saving and cost-effective

Storage Condition Store at -20ยบC Expected PCR product size : 419 bp M

500bp

419 bp

Fig1. Electrophoresis of PCR product by VetPCRTM CHV Detection Kit Lane M : BrigTM Molecular Weight Marker (Bioingentech Ltda.) Lane N : Negative control Lane 1~4 : CHV Positive sample Lane P : Positive control

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VetPCRTM CLOS.PER Detection Kit

Product

VetPCR CLOS.PER Detection Kit

DESCRIPTION Clostridium perfringens is one of the most wide spread pathogenic bacteria, and has been associated with a range of diarrheal diseases in both humans and animals. In the dog, C. perfringens has been associated with 28~34 percent of diarrheic cases. The most common clinical signs are chronic intermittent or persistent diarrhea. In some animals acute diarrhea is the primary sign. In fact, some of the cases of hemorrhagic gastroenteritis (HGE syndrome), characterized by acute bloody diarrhea and an increased packed cell volume that most practitioners have seen over the years, may have been due to C. perfringens enterotoxicosis (CPE). Many animals exhibit signs of large bowel diarrhea, but small bowel signs may be seen as well. In some cases signs may be seen for only a day or two at a time, with persistent recurrences on a weekly, monthly, or on a less frequent basis. VetPCRTM CLOS.PER Detection Kit is the direct detection of Clostridium perfringens on the basis of a genetic database, so it can diagnose very fast and accurately. It can amplify only specific gene using the PCR (Polymerase Chain Reaction) method, and take only 3 hours for detection. Therefore, it is a very fast accurate and reliable technique.

Cod. Kit

48 & 96 Test/kit VET0001F(48) or (96)

Contents TM

Kit 48

Kit 96

48 1 1 4 1 1 50

96 1 1 8 1 1 100

Characteristics Ready to use : only DNA template and D.W. are needed Easy and speed protocol Stable for 1 year at -20ยบC Time-saving and cost-effective

Storage Condition Store at -20ยบC Expected PCR product size : 645 bp M

700bp

645 bp

CANINE

Fig1. Electrophoresis of PCR product by VetPCRTM CLOS.PER Detection Kit Lane M : BrigTM Molecular Weight Marker (Bioingentech Ltda.) Lane N : Negative control Lane 1~4 : CLOS.PER Positive sample Lane P : Positive control

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VetPCRTM CPV Detection Kit

Product

VetPCR CPV Detection Kit

CANINE

DESCRIPTION Two distinct parvoviruses (CPV), are now known to infect dogs - the pathogenic CPV-2, which was recognized as a new disease of dogs and wild canines in 1978, and the “canine minute virus” (CMV, CPV-1) reported by Binn in 1970. MVC, a completely different parvovirus, had not been associated with natural disease until 1992. CMV may cause pneumonia, myocarditis and enteritis in young pups, or transplacental infections in pregnant dams, with embryo resorptions and fetal death. Confirmed infections have been reported in the USA, Sweden, Germany, and, more recently in Italy. Only about 30 cases have been reported. Canine parvovirus (CPV, CPV-2) and feline panleukopenia virus (FPV) are very closely related and are important pathogens of their respective hosts, the dog and cat. CPV-2 infects dogs and other Canidae such as wolves, coyotes, South American dogs and Asiatic raccoon dogs, but not cats. FPV and the FPV-like viruses infect both large and small cats, as well as mink, raccoons, and possibly foxes, but not dogs. However, the clear separation of a cat virus infecting only cats (FPV) and a dog virus infecting only dogs (CPV-2) is no longer certain as the original dog virus, CPV-2 was transitory, and replaced in nature by socalled “new antigenic types” (CPV-2a and CPV-2b) that infect or replicate in, and are transmitted between, dogs and cats. Clinical signs of CPV are well known, and only briefly reviewed here since they have been reviewed in several publications. Disease is often asymptomatic in older dogs or in pups that receive a low virus dose since the severity of infection is highly dose related. For example, a pup may acquire infection by CPV in a contaminated kennel, dog show, or veterinary clinic and experience only mild, o no illness. However, virus amplified in the intestine of that pup would be shed in large amounts to littermates or other susceptible dogs in contact. In contrast to the marked panleukopenia seen in cats infected with FPV, a relative lymphopenia, not panleukopenia, is often observed in dogs infected with CPV. Lymphocyte numbers decline, but there is little effect on eosinophil, basophil,monocyte, or red cell numbers. VetPCRTM CPV Detection Kit is the direct detection of Canine parvovirus type-1/ type-2 on the basis of a genetic database, so it can diagnose very fast and accurately. It can amplify only specific gene using the PCR (Polymerase Chain Reaction) method, and take only 3 hours for detection. Therefore, it is a very fast, accurate and reliable technique.

Cod. Kit

48 & 96 Test/kit VET0001F(48) or (96)

Contents TM

VetPCR CPV PCR Pre-mixture DNase/RNase-free water CPV PCR Positive control CPV PCR Positive control Pre-mixture BrigTM Molecular Weight marker Mineral Oil DNA purification kit (see step 6.1)

Kit 48

Kit 96

48 1 1 4 1 1 50

96 1 1 8 1 1 100

Characteristics Ready to use : only DNA template and D.W. are needed Easy and speed protocol Stable for 1 year at -20ºC Time-saving and cost-effective

Storage Condition Store at -20ºC Expected PCR product size : 435 bp M

500bp

435 bp

Fig1. Electrophoresis of PCR product by VetPCRTM CPV Detection Kit Lane M : BrigTM Molecular Weight Marker (Bioingentech Ltda.) Lane N : Negative control Lane P : Positive control Lane 1~3 : CPV Positive sample

Package tubes vial vial tubes vial vial tests

VetPCRTM DIRO.IM Detection Kit

Product

VetPCR DIRO.IM Detection Kit

Cod. Kit

48 & 96 Test/kit VET0001F(48) or (96)

Contents TM

VetPCR DIRO.IM PCR Pre-mixture DNase/RNase-free water DIRO.IM PCR Positive control DIRO.IM PCR Positive control Pre-mixture BrigTM Molecular Weight marker Mineral Oil DNA purification kit (see step 6.1)

Kit 48

Kit 96

48 1 1 4 1 1 50

96 1 1 8 1 1 100

Characteristics Ready to use : only DNA template and D.W. are needed Easy and speed protocol Stable for 1 year at -20ยบC Time-saving and cost-effective

Storage Condition Store at -20ยบC Expected PCR product size : 344 bp M

400bp

344 bp

CANINE

Fig1. Electrophoresis of PCR product by VetPCRTM DIRO.IM Detection Kit Lane M : BrigTM Molecular Weight Marker (Bioingentech Ltda.) Lane N : Negative control Lane P : Positive control Lane 1~3 : DIRO.IM Positive sample

Package tubes vial vial tubes vial vial tests

VetPCRTM EHR.CA Detection Kit

Product

VetPCR EHR.CA Detection Kit

DESCRIPTION

CANINE

Canine ehrlichiosis is a disease of dogs, wolves and other canids. Occurring worldwide, canine ehrlichiosis is also known by other names such as ‘tracker dog disease’, ‘tropical canine pancytopenia’, ‘canine hemorrhagic fever’ and ‘canine typhus.’ The disease is caused by Ehrlichieae, ticktransmitted, Gram-negative, obligately intracellular bacteria that infect the leukocytes of specific mammalian hosts. There are several ehrlichieae that can infect dogs but Ehrlichia canis is the most common and severe one. Disease caused by E. canis typically occurs in three phases. The initial acute phase is characterized by fever, malaise, lymphadenomegaly, splenomegaly, thrombocytopenia, leukopenia, and nonregenerative anemia. Symptoms subside in 2 to 4 weeks but are followed by a subclinical phase that persists for 2–3 months to years, during which infected dogs are carriers. Some dogs subsequently enter a chronic phase, a period when severe clinical ehrlichiosis occurs. E. canis causes ocular disease and meningitis during this phase. Ehrlichia is transmitted by the Brown Dog tick, Rhipicephalus sanguineus. The immature form of the tick feeds on an animal infected with Ehrlichia. When the tick later feeds on another animal, the Ehrlichia is passed on. VetPCRTM EHR.CA Detection Kit is the direct detection of Ehrlichia canis on the basis of a genetic database, so it can diagnose very fast and accurately. It can amplify only specific gene using the PCR (Polymerase Chain Reaction) method, and take only 3 hours for detection. Therefore, it is a very fast, accurate and reliable technique.

Cod. Kit

48 & 96 Test/kit VET0001F(48) or (96)

Contents TM

VetPCR EHR.CA PCR Pre-mixture DNase/RNase-free water EHR.CA PCR Positive control EHR.CA PCR Positive control Pre-mixture BrigTM Molecular Weight marker Mineral Oil DNA purification kit (see step 6.1)

Kit 48

Kit 96

48 1 1 4 1 1 50

96 1 1 8 1 1 100

Characteristics Ready to use : only DNA template and D.W. are needed Easy and speed protocol Stable for 1 year at -20ºC Time-saving and cost-effective

Storage Condition Store at -20ºC Expected PCR product size : 645 bp M

700bp

645 bp

Fig1. Electrophoresis of PCR product by VetPCRTM EHR.CA Detection Kit Lane M : BrigTM Molecular Weight Marker (Bioingentech Ltda.) Lane N : Negative control Lane 1~4 : EHR.CA Positive sample Lane P : Positive control

Package tubes vial vial tubes vial vial tests

VetPCRTM EHR.spp Detection Kit

Product

VetPCR EHR.spp Detection Kit

DESCRIPTION

CANINE

Canine ehrlichiosis is a disease of dogs, wolves and other canids. Occurring worldwide, canine ehrlichiosis is also known by other names such as ‘tracker dog disease’, ‘tropical canine pancytopenia’, ‘canine hemorrhagic fever’ and ‘canine typhus.’ The disease is caused by Ehrlichieae, ticktransmitted, Gram-negative, obligately intracellular bacteria that infect the leukocytes of specific mammalian hosts. There are several ehrlichieae that can infect dogs but Ehrlichia canis is the most common and severe one. Disease caused by E. canis typically occurs in three phases. The initial acute phase is characterized by fever, malaise, lymphadenomegaly, splenomegaly, thrombocytopenia, leukopenia, and nonregenerative anemia. Symptoms subside in 2 to 4 weeks but are followed by a subclinical phase that persists for 2–3 months to years, during which infected dogs are carriers. Some dogs subsequently enter a chronic phase, a period when severe clinical ehrlichiosis occurs. E. canis causes ocular disease and meningitis during this phase. Ehrlichia is transmitted by the Brown Dog tick, Rhipicephalus sanguineus. The immature form of the tick feeds on an animal infected with Ehrlichia. When the tick later feeds on another animal, the Ehrlichia is passed on. VetPCRTM EHR spp. Detection Kit is the direct detection of Ehrlichia spp. on the basis of a genetic database, so it can diagnose very fast and accurately. It can amplify only specific gene using the PCR (Polymerase Chain Reaction) method, and take only 3 hours for detection. Therefore, it is a very fast, accurate and reliable technique.

Cod. Kit

48 & 96 Test/kit VET0001F(48) or (96)

Contents TM

VetPCR EHR.spp PCR Pre-mixture DNase/RNase-free water EHR.spp PCR Positive control EHR.spp PCR Positive control Pre-mixture BrigTM Molecular Weight marker Mineral Oil DNA purification kit (see step 6.1)

Kit 48

Kit 96

48 1 1 4 1 1 50

96 1 1 8 1 1 100

Characteristics Ready to use : only DNA template and D.W. are needed Easy and speed protocol Stable for 1 year at -20ºC Time-saving and cost-effective

Storage Condition Store at -20ºC Expected PCR product size : 451 bp M

500bp

451 bp

Fig1. Electrophoresis of PCR product by VetPCRTM EHR.spp Detection Kit Lane M : BrigTM Molecular Weight Marker (Bioingentech Ltda.) Lane N : Negative control Lane 1~3 : EHR.spp Positive sample Lane P : Positive control

Package tubes vial vial tubes vial vial tests

VetPCRTM GIAR.spp Detection Kit

Product

VetPCR GIAR.spp Detection Kit

DESCRIPTION Giardia is a protozoan parasite that lives in the intestine of affected animals. It is unclear whether there are several species of this parasite or whether there is one species that affect several different animals, including people. These small parasites are very easy to miss on a fecal exam and may not be present in the stool of animals infected with the organism. Repeated fecal exams are sometimes necessary to identify this parasite. Not all animals in which infection can be demonstrated have clinical signs. This leads some people to believe that the parasite may not cause disease. Most vets think that there may just be other factors, like the animal’s immune response to the parasite that cause some animals to develop disease and not others. Clinical signs of Giardia include weight loss, inability to gain weight appropriately during growth, diarrhea, vomiting, lack of appetite and greasy appearing stools. Them most commonly used medication for Giardia infection is metronidazole. This disease may be contagious to people from infected dogs so good sanitary practices, like washing your hands after handling an infected puppy, are very important. If a family member develops similar clinical signs, a physician should be consulted.

CANINE

VetPCRTM GIAR spp. Detection Kit is the direct detection of Giardia spp. on the basis of a genetic database, so it can diagnose very fast and accurately. It can amplify only specific gene using the PCR (Polymerase Chain Reaction) method, and take only 3 hours for detection. Therefore, it is a very fast, accurate and reliable technique.

Cod. Kit

48 & 96 Test/kit VET0001F(48) or (96)

Contents TM

VetPCR GIAR.spp PCR Pre-mixture DNase/RNase-free water GIAR.spp PCR Positive control GIAR.spp PCR Positive control Pre-mixture BrigTM Molecular Weight marker Mineral Oil DNA purification kit (see step 6.1)

Kit 48

Kit 96

48 1 1 4 1 1 50

96 1 1 8 1 1 100

Characteristics Ready to use : only DNA template and D.W. are needed Easy and speed protocol Stable for 1 year at -20ºC Time-saving and cost-effective

Storage Condition Store at -20ºC Expected PCR product size : 587 bp M

600bp

587 bp

Fig1. Electrophoresis of PCR product by VetPCRTM GIAR.spp Detection Kit Lane M : BrigTM Molecular Weight Marker (Bioingentech Ltda.) Lane 1~3 : GIAR.spp Positive sample Lane P : Positive control Lane N : Negative control

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VetPCRTM LEISH.spp Detection Kit

Product

VetPCR LEISH.spp Detection Kit

DESCRIPTION Canine leishmaniasis is a parasitic worldwide zoonotic disease due to an unicellular blood protozoa of the genus Leishmania, transmitted by a tiny insect (female phlebotomidae). Leishmaniasis also affects the man and a few other mammals, but the Leishmania species involved can be very different. The phlebotome absorbs Leishmania when pumping the blood of a parasited dog, and it can retransmit them to another dog one or two weeks after the infecting meal. Man is an exceptional host; when he happens to be contaminated, he may develop a lesion at the inoculation point (cutaneous leishmaniasis), or an internal disease, called “Kala-Azar”, inducing the enlargement of the spleen and the liver. Dogs present a suspicious injury on the nose, becomes apathetic and loses weight. Most often, the loss of temporal muscles gives him the air of an old dog. Some other signs are less constant: claws lengthening, dandruff in the hair, enlargement of lymphatic nodes, nose bleed, ocular inflammation and renal troubles.

CANINE

VetPCRTM LEISH spp. Detection Kit is the direct detection of Leishmania spp. on the basis of a genetic data base, so it can diagnose very fast and accurately. It can amplify only specific gene using the PCR (Polymerase Chain Reaction) method, and take only 3 hours for detection. Therefore, it is a very fast, accurate and reliable technique.

Cod. Kit

48 & 96 Test/kit VET0001F(48) or (96)

Contents TM

VetPCR LEISH.spp PCR Pre-mixture DNase/RNase-free water LEISH.spp PCR Positive control LEISH.spp PCR Positive control Pre-mixture BrigTM Molecular Weight marker Mineral Oil DNA purification kit (see step 6.1)

Kit 48

Kit 96

48 1 1 4 1 1 50

96 1 1 8 1 1 100

Characteristics Ready to use : only DNA template and D.W. are needed Easy and speed protocol Stable for 1 year at -20ºC Time-saving and cost-effective

Storage Condition Store at -20ºC Expected PCR product size : 195 bp M

200bp

195 bp

Fig1. Electrophoresis of PCR product by VetPCRTM LEISH.spp Detection Kit Lane M : BrigTM Molecular Weight Marker (Bioingentech Ltda.) Lane N : Negative control Lane P : Positive control Lane 1~4 : LEISH.spp Positive sample

Package tubes vial vial tubes vial vial tests

VetPCRTM LEPTO.spp Detection Kit

Product

VetPCR LEPTO.spp Detection Kit

DESCRIPTION Leptospirosis is a bacterial disease caused by pathogenic members of the Genus Leptospira. The disease occurs worldwide in numerous animal hosts, including the dog. The canine disease presents as an acute infection of the kidney and liver and, sometimes, as a septicemia. Chronic kidney disease is a common sequel of infection and abortions may occur in pregnant dams. Because many aspects of the infection are poorly understood, there is the possibility that the disease in dogs may go undiagnosed. Leptospira do not multiply outside of the host and their survival depends on environmental conditions in which leptospirae are found, e.g., soil and water conditions. Leptospira are highly susceptible to drying and to pH changes - pH<6 and pH>8 are inhibitory; temperatures < 7~10ยบC and temperatures > 34~36ยบC are detrimental. Leptospira organisms survive up to 180 days in wet soil, for many months in surface water and they survive better in stagnant than in free-flowing water. The source of infection to animals is either by direct contact with infected urine, fetal and placental material or fluids, uterine discharges, or indirect contact from a contaminated environment.

CANINE

VetPCRTM LEPTO Detection Kit is the direct detection of Leptospira spp. on the basis of a genetic database, so it can diagnose very fast and accurately. It can amplify only specific gene using the PCR (Polymerase Chain Reaction) method, and take only 3 hours for detection. Therefore, it is a very fast, accurate and reliable technique.

Cod. Kit

48 & 96 Test/kit VET0001F(48) or (96)

Contents TM

VetPCR LEPTO.spp PCR Pre-mixture DNase/RNase-free water LEPTO.spp PCR Positive control LEPTO.spp PCR Positive control Pre-mixture BrigTM Molecular Weight marker Mineral Oil DNA purification kit (see step 6.1)

Kit 48

Kit 96

48 1 1 4 1 1 50

96 1 1 8 1 1 100

Characteristics Ready to use : only DNA template and D.W. are needed Easy and speed protocol Stable for 1 year at -20ยบC Time-saving and cost-effective

Storage Condition Store at -20ยบC Expected PCR product size : 348 bp M

400bp

348 bp

Fig1. Electrophoresis of PCR product by VetPCRTM LEPTO.spp Detection Kit Lane M : BrigTM Molecular Weight Marker (Bioingentech Ltda.) Lane N : Negative control Lane P : Positive control Lane 1~4 : LEPTO.spp Positive sample

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VetPCRTM MYCO.spp Detection Kit

Product

VetPCR MYCO.spp Detection Kit

DESCRIPTION Mycoplasmas are bacteria that lack a cell wall, but are enclosed by a lipid bilayer membrane. They colonize the mucous membranes of the respiratory and genital tracts as well as red blood cells, and are found in many animals and humans. Several species are pathogenic and a variety of infections such as anaemia, arthritis, infertility and respiratory disease have been attributed to infection with mycoplasmas. Mycoplasmas are approximately 0.3/0.8 um in size and readily pass through 450 and 300 nm membranes. They are fastidious in nature and some require cholesterol, sterol or urea for growth. Most Mycoplasma spp. normally appear in the upper respiratory tract. They have also been isolated at necropsy from lungs of dogs and cats with various types of pneumonia but are not generally present in the lungs of healthy animals, especially cats. Also, these are opportunistic organisms normally found in the vaginal canal. In large enough numbers, they may cause infertility, early embryonic deaths, abortion, stillbirths and weak, nonviable pups.

48 & 96 Test/kit VET0001F(48) or (96)

Contents TM

VetPCR MYCO.spp PCR Pre-mixture DNase/RNase-free water MYCO.spp PCR Positive control MYCO.spp PCR Positive control Pre-mixture BrigTM Molecular Weight marker Mineral Oil DNA purification kit (see step 6.1)

Kit 48

Kit 96

48 1 1 4 1 1 50

96 1 1 8 1 1 100

Characteristics Ready to use : only DNA template and D.W. are needed Easy and speed protocol Stable for 1 year at -20ยบC Time-saving and cost-effective

Storage Condition Store at -20ยบC Expected PCR product size : 399 bp M

VetPCRTM MYCOspp. Detection Kit is the direct detection of Mycoplasma spp. on the basis of a genetic database, so it can diagnose very fast and accurately. It can amplify only specific gene using the PCR (Polymerase Chain Reaction) method, and take only 3 hours for detection. Therefore, it is a very fast accurate and reliable technique.

Cod. Kit

400bp

399 bp

CANINE

Fig1. Electrophoresis of PCR product by VetPCRTM MYCO.spp Detection Kit Lane M : BrigTM Molecular Weight Marker (Bioingentech Ltda.) Lane P : Positive control Lane 1~3 : MYCO.spp Positive sample Lane N : Negative control

Package tubes vial vial tubes vial vial tests

VetPCRTM NEO.CAN Detection Kit

Product

VetPCR NEO.CAN Detection Kit

DESCRIPTION

CANINE

Neospora caninum is a recently discovered, apicomplexan, coccidial protozoan that asociate with neonatal neurological and neuromuscular disease in mammals such as dogs, cattle, sheep and deer. In infected neonatal dogs, progressive hind limb paresis and paralysis are the most common clinical signs. Skin involvement has only been reported in older dogs. The life cycle of this parasite consists of three stages known as tachyzoite, tissue cyst and oocyst. Tachyzoites are the rapidly multiplying form of the parasite that invades a variety of cells, producing the characteristic lesions of neosporosis in affected animals. The latent form is the tissue cyst, which contains bradyzoites and is found in peripheral and central nervous tissue. Although other animals may be potential hosts of this parasite, only dogs can serve as both definitive (ie have tachyzoites in their tissues) and intermediate (ie shed oocysts in their feces) hosts of this parasite. When a definitive host ingests tissue cysts from infected intermediate host tissues, sexual development of this parasite takes place. This results in shedding of unsporulated oocysts in the feces. Sporulation occurs outside the host. Intermediate hosts such as cattle, dogs, sheep, goats, horses and deer may then become infected by ingesting food or water contaminated with the oocysts. VetPCRTM NEO.CAN Detection Kit is the direct detection of Neospora caninum on the basis of a genetic data base, so it can diagnose very fast and accurately. It can amplify only specific gene using the PCR (Polymerase Chain Reaction) method, and take only 3 hours for detection. Therefore, it is a very fast, accurate and reliable technique.

Cod. Kit

48 & 96 Test/kit VET0001F(48) or (96)

Contents TM

VetPCR NEO.CAN PCR Pre-mixture DNase/RNase-free water NEO.CAN PCR Positive control NEO.CAN PCR Positive control Pre-mixture BrigTM Molecular Weight marker Mineral Oil DNA purification kit (see step 6.1)

Kit 48

Kit 96

48 1 1 4 1 1 50

96 1 1 8 1 1 100

Characteristics Ready to use : only DNA template and D.W. are needed Easy and speed protocol Stable for 1 year at -20ยบC Time-saving and cost-effective

Storage Condition Store at -20ยบC Expected PCR product size : 261 bp M

300bp

261 bp

Fig1. Electrophoresis of PCR product by VetPCRTM NEO.CAN Detection Kit Lane M : BrigTM Molecular Weight Marker (Bioingentech Ltda.) Lane P : Positive control Lane 1~4 : NEO.CAN Positive sample Lane N : Negative control

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VetPCRTM PAST.MU Detection Kit

Product

VetPCR PAST.MU Detection Kit

DESCRIPTION Pasteurella multocida is a Gram-negative, nonmotile, facultatively anaerobic coccobacillus that forms part of the commensal flora in the oral cavity, upper respiratory and gastrointestinal tract of domesticated and wild animals. However, it is capable of producing septicemic or respiratory diseases in animals by infecting organs and tissues that have been previously weaken by stress,injuries or other microbial infections. In addition, this bacterium also acts as an opportunistic pathogen to humans, since bite and scratch wounds from pet animals such as cats and dogs can result in severe localized infections. VetPCRTM PAST.MU Detection Kit is the direct detection of Pasteurella multocida on the basis of a genetic data base, so it can diagnose very fast and accurately. It can amplify only specific gene using the PCR (Polymerase Chain Reaction) method, and take only 3hours for detection. Therefore, it is a very fast, accurate and reliable technique.

Cod. Kit

48 & 96 Test/kit VET0001F(48) or (96)

Contents TM

VetPCR PAST.MU PCR Pre-mixture DNase/RNase-free water PAST.MU PCR Positive control PAST.MU PCR Positive control Pre-mixture BrigTM Molecular Weight marker Mineral Oil DNA purification kit (see step 6.1)

Kit 48

Kit 96

48 1 1 4 1 1 50

96 1 1 8 1 1 100

Characteristics Ready to use : only DNA template and D.W. are needed Easy and speed protocol Stable for 1 year at -20ยบC Time-saving and cost-effective

Storage Condition Store at -20ยบC Expected PCR product size : 252 bp M

300bp

252 bp

CANINE

Fig1. Electrophoresis of PCR product by VetPCRTM PAST.MU Detection Kit Lane M : BrigTM Molecular Weight Marker (Bioingentech Ltda.) Lane P : Positive control Lane 1~4 : PAST.MU Positive sample Lane N : Negative control

Package tubes vial vial tubes vial vial tests

VetPCRTM RICK.spp Detection Kit

Product

VetPCR RICK.spp Detection Kit

DESCRIPTION Bacteria of the genus Rickettsia are carried as parasites by many ticks, fleas, and lice, and cause diseases such as typhus, rickettsialpox, BrillZinsser disease, Boutonneuse fever, Rocky Mountain spotted fever, and endemic typhus in human and animals. They have also been associated with a range of plant diseases. Like viruses, they grow only in living cells. The name rickettsia is used for any member of the Rickettsiales. They are thought to be the closest living relative to the very mitochondria which inhabit each cell of all Eukaryotes, including humans, working as the energy-conversion organ for that cell. Rickettsia are non-motile, Gramnegative, non-sporeforming, highly pleomorphic organisms that can present as cocci, rods or thread-like. Obligate intracellular parasites, the Rickettsia depend on entry, growth, and replication within the cytoplasm of eukaryotic host cells (typically endothelial cells). Because of this, Rickettsia cannot live in artificial nutrient environments and are grown either in tissue or embryo cultures (typically, chicken embryos are used). In the past they were regarded as microorganisms positioned somewhere between viruses and true bacteria. The majority of Rickettsia bacteria are susceptible to antibiotics of the tetracycline group.

CANINE

VetPCRTM RICK Detection Kit is the direct detection of Rickettsia spp. on the basis of a genetic database, so it can diagnose very fast andaccurately. It can amplify only specific gene using the PCR (Polymerase Chain Reaction) method, and take only 3 hours for detection. Therefore, it is a very fast accurate and reliable technique.

Cod. Kit

48 & 96 Test/kit VET0001F(48) or (96)

Contents TM

VetPCR RICK.spp PCR Pre-mixture DNase/RNase-free water RICK.spp PCR Positive control RICK.spp PCR Positive control Pre-mixture BrigTM Molecular Weight marker Mineral Oil DNA purification kit (see step 6.1)

Kit 48

Kit 96

48 1 1 4 1 1 50

96 1 1 8 1 1 100

Characteristics Ready to use : only DNA template and D.W. are needed Easy and speed protocol Stable for 1 year at -20ยบC Time-saving and cost-effective

Storage Condition Store at -20ยบC Expected PCR product size : 384 bp M

400bp

384 bp

Fig1. Electrophoresis of PCR product by VetPCRTM RICK.spp Detection Kit Lane M : BrigTM Molecular Weight Marker (Bioingentech Ltda.) Lane N : Negative control Lane 1~4 : RICK.spp Positive sample Lane P : Positive control

Package tubes vial vial tubes vial vial tests

VetPCRTM SALMO.spp Detection Kit

Product

VetPCR SALMO.spp Detection Kit

DESCRIPTION Salmonella is a rod-shaped, motile bacterium - nonmotile exceptions S. gallinarum and S. pullorum-, nonsporeforming and Gram-negative. There is a widespread occurrence in animals, especially in poultry and swine. Environmental sources of the organism include water, soil, insects, factory surfaces, kitchen surfaces, animal feces, raw meats, raw poultry, and raw seafoods, to name only a few. Salmonellosis is a bacterial disease caused by the bacterium Salmonella. Many different kinds of Salmonella can make people sick. Most people have diarrhea, fever, and stomach pain that start 1 to 3 days after they get infected. These symptoms usually go away after 1 week. Sometimes, people have to see a doctor or go to the hospital because the diarrhea is severe or the infection has affected other organs. Salmonella infections can result in a variety of illnesses, such as pneumonia, meningitis and septicemia. The most common clinical signs for both reptiles and people include:Extreme diarrhea, vomiting and high fever.

48 & 96 Test/kit VET0001F(48) or (96)

Contents TM

VetPCR SALMO.spp PCR Pre-mixture DNase/RNase-free water SALMO.spp PCR Positive control SALMO.spp PCR Positive control Pre-mixture BrigTM Molecular Weight marker Mineral Oil DNA purification kit (see step 6.1)

Kit 48

Kit 96

48 1 1 4 1 1 50

96 1 1 8 1 1 100

Characteristics Ready to use : only DNA template and D.W. are needed Easy and speed protocol Stable for 1 year at -20ยบC Time-saving and cost-effective

Storage Condition Store at -20ยบC Expected PCR product size : 319 bp M

VetPCRTM SALMO spp. Detection Kit is the direct detection of Salmonella spp. on the basis of a genetic database, so it can diagnose very fast and accurately. It can amplify only specific gene using the PCR (Polymerase Chain Reaction) method, and take only 3 hours for detection. Therefore, it is a very fast accurate and reliable technique.

Cod. Kit

400bp

319 bp

CANINE

Fig1. Electrophoresis of PCR product by VetPCRTM SALMO.spp Detection Kit Lane M : BrigTM Molecular Weight Marker (Bioingentech Ltda.) Lane N : Negative control Lane 1~4 : SALMO.spp Positive sample Lane P : Positive control

Package tubes vial vial tubes vial vial tests

VetPCRTM TOXO.GO Detection Kit

Product

VetPCR TOXO.GO Detection Kit

DESCRIPTION

CANINE

Toxoplasmosis is the most common parasitic infection worldwide affecting humans and a number of domestic animals. The organism that causes toxoplasmosis is the obligate intracellular protozoan parasite Toxoplasma gondii. The cat is the only animal in which sexual reproduction of the organism occurs. Thus, cats are the only domestic animal that has the potential to shed the organism’s eggs. Despite the high prevalence of T. gondii infection, significant clinical disease in cats (and other species) appears to be very rare. This implies that many infected animals can be carriers of the parasite with unnoticed symptoms. When disease does occur, it may develop either following primary infection due to an inadequate immune response to stop the invasive tachyzoites, or as a result of reactivated infection due to compromised immunity. The clinical signs of toxoplasmosis in dogs are usually characterized by ataxia, diarrhoea and respiratory distress. Focal necrotic areas in the lung, liver and brain of infected dogs are common and could lead to development of several clinical signs. Therefore, toxoplasmosis in dogs and other animals can mimic many infectious diseases. Dogs may transmit Toxoplasmosis to humans. VetPCRTM TOXO.GO Detection Kit is the direct detection of Toxoplasma gondii on the basis of a genetic data base, so it can diagnose very fast and accurately. It can amplify only specific gene using the PCR (Polymerase Chain Reaction) method, and take only 3 hours for detection. Therefore, it is a very fast, accurate and reliable technique.

Cod. Kit

48 & 96 Test/kit VET0001F(48) or (96)

Contents TM

VetPCR TOXO.GO PCR Pre-mixture DNase/RNase-free water TOXO.GO PCR Positive control TOXO.GO PCR Positive control Pre-mixture BrigTM Molecular Weight marker Mineral Oil DNA purification kit (see step 6.1)

Kit 48

Kit 96

48 1 1 4 1 1 50

96 1 1 8 1 1 100

Characteristics Ready to use : only DNA template and D.W. are needed Easy and speed protocol Stable for 1 year at -20ºC Time-saving and cost-effective

Storage Condition Store at -20ºC Expected PCR product size : 462 bp M

500bp

462 bp

Fig1. Electrophoresis of PCR product by VetPCRTM TOXO.GO Detection Kit Lane M : BrigTM Molecular Weight Marker (Bioingentech Ltda.) Lane 1~3 : TOXO.GO Positive sample Lane P : Positive control Lane N : Negative control

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VetPCRTM CCoV Detection Kit DESCRIPTION

CANINE

Canine coronavirus (CCoV) is a cause of sporadic outbreaks of enteritis in dogs.The infection was first described by Binn in 1971 during an epizootic in Germany,although serological evidence was obtained earlier suggesting that dogs may be naturally infected with a coronavirus related to swine transmissible gastroenteritis virus (TGEV). CCoV is now recognized as an important, but imperfectly understood, pathogen of dogs. The incubation period is short. Vomiting and diarrhea may be seen by 1 - 3 days post-infection and, when clinical illness occurs, virus spreads rapidly. The virus is highly contagious and often may cause clinical signs in some dogs, with no illness occurring in other contact animals. Feces may be mucoid or watery, sometimes streaked with blood, and it is exceptionally malodorous. Pups become dehydrated, depressed and anorexic, even if fluid therapy is started early. There is generally no fever, although elevated body temperatures have been observed in some cases. VetPCRTM CCoV Detection Kit is the direct detection of Canine coronavirus on the basis of a genetic database, so it can diagnose very fast and accurately. It can amplify only specific gene using the PCR (Polymerase Chain Reaction) method, and take only 3hours for detection. Therefore, it is a very fast, accurate and reliable technique.

Product

VetPCRTM CCoV Detection Kit

48 & 96 Test/kit

Contents VetPCRTM CCoV RT-PCR Pre-mixture VetPCRTM CCoV PCR Pre-mixture BrigRT-PCRTM solution BiotechTM Transcriptase solution DNase/RNase-free water CCoV RT-PCR Positive control CCoV RT-PCR Positive control Pre-mixture CCoV PCR Positive control Pre-mixture BrigTM Molecular Weight marker Mineral Oil RNA extraction kit (see step 6.1) Characteristics

Kit 48 48 48 1 1 1 1 4 4 1 1 50

Cod. Kit VET0001F(48) or (96) Kit 96 96 96 1 1 1 1 8 8 1 2 100

Ready to use : only DNA template and D.W. are needed Easy and speed protocol Stable for 1 year at -20ยบC Time-saving and cost-effective

Storage Condition Store at -20ยบC Expected PCR product size : 622 bp M P 1 2 700bp

N 622 bp

Fig1. Electrophoresis of PCR product by VetPCRTM CCoV Detection Kit Lane M : BrigTM Molecular Weight Marker (Bioingentech Ltda.) Lane P : Positive control Lane 1~4 : CCoV Positive sample Lane N : Negative control

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VetPCRTM CDV Detection Kit DESCRIPTION Canine distemper is a highly infectious, acute or subacute, febrile viral disease of dogs and other carnivores, which occurs world-wide. The disease is caused by canine distemper virus (CDV), a member of the genus morbillivirus which belongs to the paramyxovirus family. There is great variation in the duration and severity of clinical disease. Signs may range from no visible signs to severe disease, with or without CNS signs, with approximately 50% mortality. The first pyrexia (3 - 6 DPI) may pass unnoticed, the second peak (several days later and intermittent thereafter) is usually connected with serous (later mucopurulent) nasal and ocular discharge, depression and anorexia. Lymphopenia is always present during the early infection. Gastrointestinal and/or respiratory signs may follow, often enhanced by secondary bacterial infection. However, about 50% of CDV infections in dogs are probably subclinical or very mild. Some dogs develop CNS signs, often, but not always, following systemic disease. Depending on the virus strain, the signs may be more related to acute grey matter or subacute white matter disease.

CANINE

VetPCRTM CDV Detection Kit is the direct detection of Canine distemper virus on the basis of a genetic data base, so it can diagnose very fast and accurately. It can amplify only specific gene using the PCR (Polymerase Chain Reaction) method, and take only 3hours for detection. Therefore, it is a very fast accurate and reliable technique.

Product VetPCRTM CDVDetection Kit

Product 48 & 96 Test/kit

Contents VetPCRTM CDV RT-PCR Pre-mixture VetPCRTM CDV PCR Pre-mixture BrigRT-PCRTM solution BiotechTM Transcriptase solution DNase/RNase-free water CDV RT-PCR Positive control CDV RT-PCR Positive control Pre-mixture CDV PCR Positive control Pre-mixture BrigTM Molecular Weight marker Mineral Oil RNA extraction kit (see step 6.1) Characteristics

Kit 48 48 48 1 1 1 1 4 4 1 1 50

Cod. Kit VET0001F(48) or (96) Kit 96 96 96 1 1 1 1 8 8 1 2 100

Ready to use : only DNA template and D.W. are needed Easy and speed protocol Stable for 1 year at -20ยบC Time-saving and cost-effective

Storage Condition Store at -20ยบC Expected PCR product size : 329 bp M P 1 2

400bp

329 bp

Fig1. Electrophoresis of PCR product by VetPCRTM CDV Detection Kit Lane M : BrigTM Molecular Weight Marker (Bioingentech Ltda.) Lane P : Positive control Lane 1~3 : CDV Positive sample Lane N : Negative control

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VetPCRTM CPIV Detection Kit DESCRIPTION Canine parainfluenza virus is similar to the human common cold virus. Alone, it is not very dangerous, however, infected animals become very prone to secondary infections that often lead to severe respiratory infections. Kennel Cough is an infection of the dog’s upper respiratory tract and main lower airways. It can be caused by a number of different bacteria or viruses. Kennel Cough is rarely fatal but is very distressing for dog and owner. The most common sign is a dry, harsh, hacking cough due to inflammation of the voicebox, bronchial tubes, and windpipe. Sometimes the dog coughs so hard it gags. The coughing is usually made worse by exercise, excitement or pressure on the throat region. The symptoms generally occur 2-8 days after exposure to an infected animal. In particularly bad cases the animals may have a fever and poor appetite. Because the respiratory system is in a weakened state, these dogs are also at risk of developing pneumonia. VetPCRTM CPIV Detection Kit is the direct detection of Canine parainfluenza virus on the basis of a genetic database, so it can diagnose very fast and accurately. It can amplify only specific gene using the PCR (Polymerase Chain Reaction) method, and take only 3 hours for detection. Therefore,it is a very fast, accurate and reliable technique.

Product

VetPCRTM CPIV Detection Kit

48 & 96 Test/kit

Contents VetPCRTM CPIV RT-PCR Pre-mixture VetPCRTM CPIV PCR Pre-mixture BrigRT-PCRTM solution BiotechTM Transcriptase solution DNase/RNase-free water CPIV RT-PCR Positive control CPIV RT-PCR Positive control Pre-mixture CPIV PCR Positive control Pre-mixture BrigTM Molecular Weight marker Mineral Oil RNA extraction kit (see step 6.1) Characteristics

Kit 48 48 48 1 1 1 1 4 4 1 1 50

Cod. Kit VET0001F(48) or (96) Kit 96 96 96 1 1 1 1 8 8 1 2 100

Ready to use : only DNA template and D.W. are needed Easy and speed protocol Stable for 1 year at -20ºC Time-saving and cost-effective

Storage Condition Store at -20ºC Expected PCR product size : 408 bp M

500bp

408 bp

CANINE

Fig1. Electrophoresis of PCR product by VetPCRTM CPIV Detection Kit Lane M : BrigTM Molecular Weight Marker (Bioingentech Ltda.) Lane P : Positive control Lane 1~4 : CPIV Positive sample Lane N : Negative control

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VetPCRTM RV Detection Kit DESCRIPTION Rabies is a zoonotic viral disease which infects domestic and wild animals. It is transmitted to other animals and humans through close contact with saliva from infected animals (i.e. bites, scratches, licks on broken skin and mucous membranes). Once symptoms of the disease develop, rabies is fatal to both animals and humans. The first symptoms of rabies are usually non-specific and suggest involvement of the respiratory, gastrointestinal and/or central nervous systems. In the acute stage, signs of hyperactivity (furious rabies) or paralysis (dumb rabies) predominate. In both furious and dumb rabies, paralysis eventually progresses to complete paralysis followed by coma and death in all cases, usually due to respiratory failure. Without intensive care, death occurs during the first seven days of illness. VetPCRTM RV Detection Kit is the direct detection of Rabies virus on the basis of a genetic database, so it can diagnose very fast and accurately. It can amplify only specific gene using the PCR (Polymerase Chain Reaction) method, and take only 3 hours for detection. Therefore, it is a very fast, accurate and reliable technique.

Product

VetPCRTM RV Detection Kit

48 & 96 Test/kit

Contents VetPCRTM RV RT-PCR Pre-mixture VetPCRTM RV PCR Pre-mixture BrigRT-PCRTM solution BiotechTM Transcriptase solution DNase/RNase-free water RV RT-PCR Positive control RV RT-PCR Positive control Pre-mixture RV PCR Positive control Pre-mixture BrigTM Molecular Weight marker Mineral Oil RNA extraction kit (see step 6.1) Characteristics

Cod. Kit VET0001F(48) or (96) Kit 96

Kit 48 48 48 1 1 1 1 4 4 1 1 50

96 96 1 1 1 1 8 8 1 2 100

Ready to use : only DNA template and D.W. are needed Easy and speed protocol Stable for 1 year at -20ยบC Time-saving and cost-effective

Storage Condition Store at -20ยบC Expected PCR product size : 404 bp M

500bp

404 bp

CANINE

Fig1. Electrophoresis of PCR product by VetPCRTM RV Detection Kit Lane M : BrigTM Molecular Weight Marker (Bioingentech Ltda.) Lane 1~3 : RV Positive sample Lane P : Positive control Lane N : Negative control

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VetPCRTM BAB.EQUI Detection Kit

Product

VetPCR BAB.EQUI Detection Kit

EQUINE

DESCRIPTION Equine piroplasmosis is caused by the intracellular, haemoprotozoan parasites Babesia equi (recently reclassified as Theileria equi) and Babesia caballi, which are transmitted by ticks of several genera. The disease is found in many tropical and subtropical areas. Clinical manifestation of the disease is variable and often includes icterus (jaundice), haemoglobinuria and fever. Both chronic and acute infection can occur. Sub-clinical infected animals are of major concern, as they can be carriers of the organism. In addition to the fact that subclinical babesiosis may negatively affect the animal’s performance, it has been shown that strenuous exercise, such as that experienced in horse racing, can cause sub-clinical infections to become acute. Thus there is a real need for the diagnosis of both clinical and sub-clinical infections. In general, B. caballi causes a less severe disease, as only about 1% of the red blood cells are infected. Infections may not be apparent, but can persist 1 to 4 years before they are eventually eliminated. They may be associated with poor appetite, poor performance and weight loss. In contrast, B. equi infects up to 20% of red blood cells, leading to more severe clinical signs including fever, anemia, icterus, increased respiratory and heart rates and enlargement of the spleen. The parasites destroy red blood cells, causing anemia, and the released hemoglobin may cause icterus and dark urine. Colic, constipation followed by diarrhea, and swelling of the legs can occur. Foals can be infected in utero, and can be aborted or born anemic and weak. Animals with B. equi infections become life-long carriers. VetPCRTM BAB.EQUI Detection Kit is the direct detection of Babesia equi on the basis of a genetic database, so it can diagnose very fast and accurately. It can amplify only specific gene using the PCR (Polymerase Chain Reaction) method, and take only 3 hours for detection. Therefore, it is a very fast, accurate and reliable technique.

Cod. Kit

48 & 96 Test/kit VET0001F(48) or (96)

Contents

Kit 48

Kit 96

48 1 1 4 1 1 50

96 1 1 8 1 1 100

VetPCR BAB.EQUI PCR Pre-mixture DNase/RNase-free water BAB.EQUI PCR Positive control BAB.EQUI PCR Positive control Pre-mixture BrigTM Molecular Weight marker Mineral Oil DNA purification kit (see step 6.1)

Characteristics Ready to use : only DNA template and D.W. are needed Easy and speed protocol Stable for 1 year at -20ºC Time-saving and cost-effective

Storage Condition Store at -20ºC Expected PCR product size : 323 bp M

400bp

323 bp

Fig1. Electrophoresis of PCR product by VetPCRTM BAB.EQUI Detection Kit Lane M : BrigTM Molecular Weight Marker (Bioingentech Ltda.) Lane N : Negative control Lane 1~4 : BAB.EQUI Positive sample Lane P : Positive control

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VetPCRTM BAB.CAB Detection Kit

Product

VetPCR BAB.CAB Detection Kit

EQUINE

DESCRIPTION Equine piroplasmosis is caused by the intracellular, haemoprotozoan parasites Babesia equi (recently reclassified as Theileria equi) and Babesia caballi, which are transmitted by ticks of several genera. The disease is found in many tropical and subtropical areas. Clinical manifestation of the disease is variable and often includes icterus (jaundice), haemoglobinuria and fever. Both chronic and acute infection can occur. Sub-clinical infected animals are of major concern, as they can be carriers of the organism. In addition to the fact that subclinical babesiosis may negatively affect the animal’s performance, it has been shown that strenuous exercise, such as that experienced in horse racing, can cause sub-clinical infections to become acute. Thus there is a real need for the diagnosis of both clinical and sub-clinical infections. In general, B. caballi causes a less severe disease, as only about 1% of the red blood cells are infected. Infections may not be apparent, but can persist 1 to 4 years before they are eventually eliminated. They may be associated with poor appetite, poor performance and weight loss. In contrast, B. equi infects up to 20% of red blood cells, leading to more severe clinical signs including fever, anemia, icterus, increased respiratory and heart rates and enlargement of the spleen. The parasites destroy red blood cells, causing anemia, and the released hemoglobin may cause icterus and dark urine. Colic, constipation followed by diarrhea, and swelling of the legs can occur. Foals can be infected in utero, and can be aborted or born anemic and weak. Animals with B. equi infections become life-long carriers. VetPCRTM BAB.CAB Detection Kit is the direct detection of Babesia caballi on the basis of a genetic database, so it can diagnose very fast and accurately. It can amplify only specific gene using the PCR (Polymerase Chain Reaction) method, and take only 3 hours for detection. Therefore, it is a very fast, accurate and reliable technique.

Cod. Kit

48 & 96 Test/kit VET0001F(48) or (96)

Contents

Kit 48

Kit 96

48 1 1 4 1 1 50

96 1 1 8 1 1 100

VetPCR BAB.CAB PCR Pre-mixture DNase/RNase-free water BAB.CAB PCR Positive control BAB.CAB PCR Positive control Pre-mixture BrigTM Molecular Weight marker Mineral Oil DNA purification kit (see step 6.1)

Characteristics Ready to use : only DNA template and D.W. are needed Easy and speed protocol Stable for 1 year at -20ºC Time-saving and cost-effective

Storage Condition Store at -20ºC Expected PCR product size : 311 bp M

400bp

311 bp

Fig1. Electrophoresis of PCR product by VetPCRTM BAB.CAB Detection Kit Lane M : BrigTM Molecular Weight Marker (Bioingentech Ltda.) Lane P : Positive control Lane 1~4 : BAB.CAB Positive sample Lane N : Negative control

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VetPCRTM EHV-1 Detection Kit

Product

VetPCR EHV-1 Detection Kit

DESCRIPTION

EQUINE

Equine herpesvirus type I (EHV-1) is a member of the Alphaherpesvirinae subfamily. EHV-1 is a major cause worldwide of epidemic abortion, perinatal mortality, respiratory disease and neurological disorders in horses. As with other herpesvirus infections, lifelong latency of the virus in the nervous system of the host occurs, and periodical reactivation of the virus can cause new outbreaks. Upper respiratory infection is the most common manifestation of EHV-1 infection. Commonly, young horses have depression, poor appetite, nasal discharge and cough. If young horses are housed or pastured together, many horses in the herd may experience disease from which they recover uneventfully. Disease may be mild or unapparent in older horses. Neurological signs occur infrequently as a result of EHV-1 infection; however, outbreaks of neurologic disease associated with fever, nasal discharge and cough have been reported in the USA and elsewhere. Neurologic signs may include incoordination that can progress to an inability to stand. Horses may be unable to urinate or may dribble small volumes of urine. Horses may have difficulty producing manure. Sometimes the neurologic signs are accompanied by cellulitis (inflammation or swelling of the limbs) and petechiae (small hemorrhages on the gums). VetPCRTM EHV-1 Detection Kit is the direct detection of Equine herpesvirus types-1 on the basis of a genetic database, so it can diagnose very fast and accurately. It can amplify only specific gene using the PCR (Polymerase Chain Reaction) method, and take only 3 hours for detection. Therefore, it is a very fast, accurate and reliable technique.

Cod. Kit

48 & 96 Test/kit VET0001F(48) or (96)

Contents

Kit 48

Kit 96

48 1 1 4 1 1 50

96 1 1 8 1 1 100

VetPCR EHV-1 PCR Pre-mixture DNase/RNase-free water EHV-1 PCR Positive control EHV-1 PCR Positive control Pre-mixture BrigTM Molecular Weight marker Mineral Oil DNA purification kit (see step 6.1)

Characteristics Ready to use : only DNA template and D.W. are needed Easy and speed protocol Stable for 1 year at -20ยบC Time-saving and cost-effective

Storage Condition Store at -20ยบC Expected PCR product size : 290 bp M

300bp

290 bp

Fig1. Electrophoresis of PCR product by VetPCRTM EHV-1 Detection Kit Lane M : BrigTM Molecular Weight Marker (Bioingentech Ltda.) Lane 1~3 : EHV-1 Positive sample Lane P : Positive control Lane N : Negative control

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VetPCRTM EHV-4 Detection Kit

Product

VetPCR EHV-4 Detection Kit

DESCRIPTION Equine herpesvirus type IV (EHV-4) is a virus of the Alphaherpesvirinae subfamily. Equine herpesvirus type I (EHV-1) and EHV-4 are remarkably ubiquitous in the world’s horse population and are primary respiratory pathogens that can cause respiratory tract disease without major predisposing factors. EHV-4 produce an acute febrile respiratory disease on primary infection, characterized by rhinopharyngitis and tracheobronchitis. Outbreaks of respiratory disease occur annually among foals in areas with concentrated horse populations; elsewhere, episodes are sporadic. Most of these outbreaks in weanlings are caused by strains of EHV-4. The age, seasonal, and geographic distributions vary and probably are determined by immune status and concentration of horses. In individual horses, the outcome of exposure is determined by viral strain involved, immune status, pregnancy status, and possibly age. Infection of pregnant mares with EHV-4 strains rarely results in abortion.

48 & 96 Test/kit VET0001F(48) or (96)

Contents

Kit 48

Kit 96

48 1 1 4 1 1 50

96 1 1 8 1 1 100

VetPCR EHV-4 PCR Pre-mixture DNase/RNase-free water EHV-4 PCR Positive control EHV-4 PCR Positive control Pre-mixture BrigTM Molecular Weight marker Mineral Oil DNA purification kit (see step 6.1)

Characteristics Ready to use : only DNA template and D.W. are needed Easy and speed protocol Stable for 1 year at -20ºC Time-saving and cost-effective

Storage Condition Store at -20ºC Expected PCR product size : 292 bp M

VetPCRTM EHV-4 Detection Kit is the direct detection of Equine herpesvirus types-4 on the basis of a genetic database, so it can diagnose very fast and accurately. It can amplify only specific gene using the PCR (Polymerase Chain Reaction) method, and take only 3 hours for detection. Therefore, it is a very fast, accurate and reliable technique.

Cod. Kit

300bp

292 bp

EQUINE

Fig1. Electrophoresis of PCR product by VetPCRTM EHV-4 Detection Kit Lane M : BrigTM Molecular Weight Marker (Bioingentech Ltda.) Lane N : Negative control Lane 1~4 : EHV-4 Positive sample Lane P : Positive control

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VetPCRTM EHV-5 Detection Kit

Product

VetPCR EHV-5 Detection Kit

DESCRIPTION Equine herpesvirus type V (EHV-5) classified as members of the Gammaherpesvirinae subfamily. The pathogenic significance of the equine gammaherpesviruses (EHV-2 and 5) is uncertain, but they may cause upper respiratory tract disease, generalized malaise and fever, pharyngitis, and enlarged lymph nodes in young horses. Studies have shown that EHV-5 infection is very common in young horses and EHV-5 infections persist in young horses for at least several months. However, EHV-5 is not specifically associated with respiratory disease. In contrast, EHV-2 is less commonly isolated from young horses and its detection is associated with the occurrence of mild respiratory disease. VetPCRTM EHV-5 Detection Kit is the direct detection of Equine herpesvirus types-5 on the basis of a genetic database, so it can diagnose very fast and accurately. It can amplify only specific gene using the PCR (Polymerase Chain Reaction) method, and take only 3 hours for detection. Therefore, it is a very fast, accurate and reliable technique.

Cod. Kit

48 & 96 Test/kit VET0001F(48) or (96)

Contents TM

VetPCR EHV-5 PCR Pre-mixture DNase/RNase-free water EHV-5 PCR Positive control EHV-5 PCR Positive control Pre-mixture BrigTM Molecular Weight marker Mineral Oil DNA purification kit (see step 6.1)

Kit 48

Kit 96

48 1 1 4 1 1 50

96 1 1 8 1 1 100

Characteristics Ready to use : only DNA template and D.W. are needed Easy and speed protocol Stable for 1 year at -20ยบC Time-saving and cost-effective

Storage Condition Store at -20ยบC Expected PCR product size : 355 bp M

400bp

355 bp

EQUINE

Fig1. Electrophoresis of PCR product by VetPCRTM EHV-5 Detection Kit Lane M : BrigTM Molecular Weight Marker (Bioingentech Ltda.) Lane N : Negative control Lane P : Positive control Lane 1~3 : EHV-5 Positive sample

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VetPCRTM LAW.INTRA Detection Kit

Product

VetPCR LAW.INTRA Detection Kit

DESCRIPTION Proliferative enteropathy (PE), also known as proliferative ileitis, is caused by infection with Lawsonia intracellularis, an obligate intracellular, curve-shaped, argyrophilic bacterium. The disease has been detected in domestic and laboratory animals including primates, pig, horse, dog, rat, ferret, guinea pig, rabbit and hamster. This agent causes proliferation of intestinal cells, resulting in enteric disease which is sometimes fatal. The disease is responsible for serious economic loss to animal production worldwide. L. intracellularis is an obligate intracellular bacterium. Animals suffering from chronic PE may have clinical or sub-clinical effects on weight gain, feed conversion and fecal consistency. Diarrhea is a common symptom. VetPCRTM LAW.INTRA Detection Kit is the direct detection of Lawsonia intracellularis on the basis of a genetic database, so it can diagnose very fast and accurately. It can amplify only specific gene using the PCR (Polymerase Chain Reaction) method, and take only 3 hours for detection. Therefore, it is a very fast, accurate and reliable technique.

Cod. Kit

48 & 96 Test/kit VET0001F(48) or (96)

Contents

Kit 48

Kit 96

48 1 1 4 1 1 50

96 1 1 8 1 1 100

VetPCR LAW.INTRA PCR Pre-mixture DNase/RNase-free water LAW.INTRA PCR Positive control LAW.INTRA PCR Positive control Pre-mixture BrigTM Molecular Weight marker Mineral Oil DNA purification kit (see step 6.1)

Characteristics Ready to use : only DNA template and D.W. are needed Easy and speed protocol Stable for 1 year at -20ยบC Time-saving and cost-effective

Storage Condition Store at -20ยบC Expected PCR product size : 261 bp M

300bp

261 bp

EQUINE

Fig1. Electrophoresis of PCR product by VetPCRTM LAW.INTRA Detection Kit Lane M : BrigTM Molecular Weight Marker (Bioingentech Ltda.) Lane P : Positive control Lane 1~4 : LAW.INTRA Positive sample Lane N : Negative control

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VetPCRTM LEP.INTE Detection Kit

Product

VetPCR LEP.INTE Detection Kit

DESCRIPTION

EQUINE

Leptospirosis is a disease of worldwide significance that infects many domestic and wildlife animal species and humans. The scientific name of the infecting organism is Leptospira interrogans, a flexible, spiral-shaped, Gram-negative spirochete with internal flagella; within this species there are many different strains (serovars). L. interrogans can be spread between animals through contact with infected urine, venereal or placental transfer, or bite wounds. Indirect transmission occurs through exposure of susceptible animals to contaminated water sources, food, bedding or human hands. This bacteria can survive in manure up to 2 months. Stagnant or slow moving water provides a suitable habitat; the organism can survive up to 20 days in water. This explains why outbreaks often increase during periods of flooding. Fever, shivering and muscle tenderness are among the first signs of acute infection. Rapid dehydration may develop subsequently. In subacute infections, the animal usually develops a fever, anorexia, dehydration, and increased thirst. Animals with liver involvement may develop icterus. Conjunctivitis and recurrent uveitis may occur in chronic infections. Breeding success can be impacted because of increased risk of abortion and decreased milk production in mares. VetPCRTM LEP.INTE Detection Kit is the direct detection of Leptospira interrogans on the basis of a genetic database, so it can diagnose very fast and accurately. It can amplify only specific gene using the PCR (Polymerase Chain Reaction) method, and take only 3 hours for detection. Therefore, it is a very fast, accurate and reliable technique.

Cod. Kit

48 & 96 Test/kit VET0001F(48) or (96)

Contents

Kit 48

Kit 96

48 1 1 4 1 1 50

96 1 1 8 1 1 100

VetPCR LEP.INTE PCR Pre-mixture DNase/RNase-free water LEP.INTE PCR Positive control LEP.INTE PCR Positive control Pre-mixture BrigTM Molecular Weight marker Mineral Oil DNA purification kit (see step 6.1)

Characteristics Ready to use : only DNA template and D.W. are needed Easy and speed protocol Stable for 1 year at -20ยบC Time-saving and cost-effective

Storage Condition Store at -20ยบC Expected PCR product size : 308 bp M

400bp

308 bp

Fig1. Electrophoresis of PCR product by VetPCRTM LEP.INTE Detection Kit Lane M : BrigTM Molecular Weight Marker (Bioingentech Ltda.) Lane P : Positive control Lane 1~4 : LEP.INTE Positive sample Lane N : Negative control

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VetPCRTM RHOD.EQUI Detection Kit

Product

VetPCR RHOD.EQUI Detection Kit

DESCRIPTION Rhodococcus equi is a Gram-positive, pleomorphic coccobacillus that is a frequent cause of pneumonia and enteritis in foals, especially before 6 months of age. It has also been linked to a variety of suppurative processes in immune-suppressed humans. The organism has a worldwide distribution and can easily be isolated from soil and environmental samples. The onset of clinical signs of R. equi pneumonia in foals is often subtle, and the infection is usually not recognized until severe abscessation has occurred. By that time, prognosis is poor. Culture of the organism from tracheal wash was previously considered the “gold standard” for diagnosis. However, it can be difficult to reliably culture R. equi from a tracheal wash sample, possibly because of prior antibiotic administration or the presence of multiple pathogenic bacterial species.

Cod. Kit

48 & 96 Test/kit VET0001F(48) or (96)

Contents TM

VetPCR RHOD.EQUI PCR Pre-mixture DNase/RNase-free water RHOD.EQUI PCR Positive control RHOD.EQUI PCR Positive control Pre-mixture BrigTM Molecular Weight marker Mineral Oil DNA purification kit (see step 6.1)

Kit 48

Kit 96

48 1 1 4 1 1 50

96 1 1 8 1 1 100

Characteristics Ready to use : only DNA template and D.W. are needed Easy and speed protocol Stable for 1 year at -20ºC Time-saving and cost-effective

Storage Condition Store at -20ºC

VetPCRTM RHOD.EQUI Detection Kit is the direct detection of Rhodococcus equi on the basis of a genetic database, so it can diagnose very fast and accurately. It can amplify only specific gene using the PCR (Polymerase Chain Reaction) method, and take only 3 hours for detection. Therefore, it is a very fast, accurate and reliable technique.

Expected PCR product size : 265 bp M

300bp

265 bp

EQUINE

Fig1. Electrophoresis of PCR product by VetPCRTM RHOD.EQUI Detection Kit Lane M : BrigTM Molecular Weight Marker (Bioingentech Ltda.) Lane P : Positive control Lane 1~3 : RHOD.EQUI Positive sample Lane N : Negative control

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VetPCRTM SALMO.spp Detection Kit

Product

VetPCR SALMO.spp Detection Kit

DESCRIPTION

EQUINE

Salmonella are facultative anaerobic, non-spore forming, gramnegative rods of the family Enterobacteriaceae. There are more than 2200 serovars of Salmonella and all are considered possible pathogens for adult horses. The most commonly isolated serovars are Salmonella typhimurium, S. agona, S. anatum, S. krefeld, S. newport, and S. saint-paul. Enteric salmonellosis is characterized by an acute colitis that results in profuse diarrhea and, occasionally, abdominal pain. Horses with enteric salmonellosis often have signs compatible with endotoxemia, and suffer from cardio-vascular shock and coagulopathies. Horses are usually febrile, tachycardic, moderately to severely obtunded, and dehydrated. Other clinical signs include fever and leukopenia, colic and proximal enteritis with gastric reflux, dark red or purple mucous membrane, abdominal pain, and abdominal distention. It is important recognize that the salmonellosis in horses can develop without diarrhea. Complications in animals with enteric salmonellosis include laminitis, bacteremia and septicemia, renal failure, thrombophlebitis, disseminated intra-vascular coagulation, hepatitis, and fungal pneumonia secondary to compromise of the intestinal barrier and the immune system. VetPCRTM SALMO.spp Detection Kit is the direct detection of Salmonella spp. on the basis of a genetic database, so it can diagnose very fast and accurately. It can amplify only specific gene using the PCR (Polymerase Chain Reaction) method, and take only 3 hours for detection. Therefore, it is a very fast, accurate and reliable technique.

Cod. Kit

48 & 96 Test/kit VET0001F(48) or (96)

Contents TM

Kit 48

Kit 96

48 1 1 4 1 1 50

96 1 1 8 1 1 100

Characteristics Ready to use : only DNA template and D.W. are needed Easy and speed protocol Stable for 1 year at -20ยบC Time-saving and cost-effective

Storage Condition Store at -20ยบC Expected PCR product size : 319 bp M

400bp

319 bp

Fig1. Electrophoresis of PCR product by VetPCRTM SALMO.spp Detection Kit Lane M : BrigTM Molecular Weight Marker (Bioingentech Ltda.) Lane N : Negative control Lane P : Positive control Lane 1~4 : SALMO.spp Positive sample

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VetPCRTM SARC.NEU Detection Kit

Product

VetPCR SARC.NEU Detection Kit

DESCRIPTION Equine Protozoal Myeloencephalitis (EPM) is an infection of the central nervous system that can affect equines of any age and sex. EPM is one of the most challenging and exasperating diseases in horses, not only for veterinary scientists but for horse owners as well. The primary parasite that causes the disease is the single-celled protozoan parasite Sarcocystis neurona. The clinical signs of EPM are a result of direct neuronal damage early in the disease and the secondary inflammatory reaction. The neurologic signs that it causes are most commonly asymmetric incoordination (ataxia), weakness and spasticity, although they may mimic almost any neurologic condition. VetPCRTM SARC.NEU Detection Kit is the direct detection of Sarcocystis neurona on the basis of a genetic database, so it can diagnose very fast and accurately. It can amplify only specific gene using the PCR (Polymerase Chain Reaction) method, and take only 3 hours for detection. Therefore, it is a very fast, accurate and reliable technique.

Cod. Kit

48 & 96 Test/kit VET0001F(48) or (96)

Contents

Kit 48

Kit 96

48 1 1 4 1 1 50

96 1 1 8 1 1 100

VetPCR SARC.NEU PCR Pre-mixture DNase/RNase-free water SARC.NEU PCR Positive control SARC.NEU PCR Positive control Pre-mixture BrigTM Molecular Weight marker Mineral Oil DNA purification kit (see step 6.1)

Characteristics Ready to use : only DNA template and D.W. are needed Easy and speed protocol Stable for 1 year at -20ยบC Time-saving and cost-effective

Storage Condition Store at -20ยบC Expected PCR product size : 405 bp M

500bp

405 bp

EQUINE

Fig1. Electrophoresis of PCR product by VetPCRTM SARC.NEU Detection Kit Lane M : BrigTM Molecular Weight Marker (Bioingentech Ltda.) Lane 1~4 : SARC.NEU Positive sample Lane P : Positive control Lane N : Negative control

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VetPCRTM STREP.EQUI Detection Kit

Product

VetPCR STREP.EQUI Detection Kit

DESCRIPTION Streptococcus equi subspecie equi is the etiologic agent of strangles and is responsible for nearly 30% of all reported equine infections worldwide. Strangles is characterized by pharyngeal constriction in the horse’s upper respiratory tract as a consequence of lymph node swelling and is often accompanied by abscessation. The very closely related organism Streptococcus zooepidemicus (S. equi subspecie zooepidemicus) is a significant cause of equine lower airway disease, foal pneumonia, endometritis, and abortion. There is currently no effective vaccine or treatment for strangles. In the past, identification of S. equi bacteria usually relied on culture of the bacteria, but this technique is slow and not very sensitive. A recent study has shown that repeated nasopharyngeal swabbing and culture of Streptococcus equi could not detect the development of healthy carriers in more than 50% of strangles outbreaks. VetPCRTM STREP.EQUI Detection Kit is the direct detection of Streptococcus equi on the basis of a genetic database, so it can diagnose very fast and accurately. It can amplify only specific gene using the PCR (Polymerase Chain Reaction) method, and take only 3 hours for detection. Therefore, it is a very fast, accurate and reliable technique.

Cod. Kit

48 & 96 Test/kit VET0001F(48) or (96)

Contents TM

VetPCR STREP.EQUI PCR Pre-mixture DNase/RNase-free water STREP.EQUI PCR Positive control STREP.EQUI PCR Positive control Pre-mixture BrigTM Molecular Weight marker Mineral Oil DNA purification kit (see step 6.1)

Kit 48

Kit 96

48 1 1 4 1 1 50

96 1 1 8 1 1 100

Characteristics Ready to use : only DNA template and D.W. are needed Easy and speed protocol Stable for 1 year at -20ºC Time-saving and cost-effective

Storage Condition Store at -20ºC Expected PCR product size : 397 bp M

400bp

397 bp

EQUINE

Fig1. Electrophoresis of PCR product by VetPCRTM STREP.EQUI Detection Kit Lane M : BrigTM Molecular Weight Marker (Bioingentech Ltda.) Lane N : Negative control Lane P : Positive control Lane 1~4 : STREP.EQUI Positive sample

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VetPCRTM EAV Detection Kit DESCRIPTION Equine arteritis virus (EAV) is a positive stranded RNA virus in the family Arteriviridae, order Nidovirales, and infects horses worldwide. Sporadic respiratory disease and sudden death in foals, abortion in mares, and mild or subclinical infections in adult horses have been described resulting from this infection. Adult stallions may become chronically infected; they can become a reservoir and spread the virus via their semen. Clinical signs of EAV infection include depression, anorexia, jaundice, rash, nasal discharge, cough, swelling of the lower limbs, scrotum or mammary gland, periobital swelling and conjunctivitis (pink eye). Mares and geldings eliminate infection within 21 to 30 days. However, 30-60 % of infected stallions become carrriers and shed the virus in their semen. Only one serotype of EAV is reported, but there are several strains that differ in their virulence and neutralization phenotype. VetPCRTM EAV Detection Kit is the direct detection of Equine arteritis virus on the basis of a genetic database, so it can diagnose very fast and accurately. It can amplify only specific gene using the PCR (Polymerase Chain Reaction) method, and take only 4 hours for detection. Therefore, it is a very fast, accurate and reliable technique.

Product VetPCRTM EAV Detection Kit

Contents VetPCRTM EAV RT-PCR Pre-mixture VetPCRTM EAV PCR Pre-mixture BrigRT-PCRTM solution BiotechTM Transcriptase solution DNase/RNase-free water EAV RT-PCR Positive control EAV RT-PCR Positive control Pre-mixture EAV PCR Positive control Pre-mixture BrigTM Molecular Weight marker Mineral Oil RNA extraction kit (see step 6.1) Characteristics

Kit 48 48 48 1 1 1 1 4 4 1 1 50

Cod. Kit VET0001F(48) or (96) Kit 96 96 96 1 1 1 1 8 8 1 2 100

Ready to use : only DNA template and D.W. are needed Easy and speed protocol Stable for 1 year at -20ยบC Time-saving and cost-effective

Storage Condition Store at -20ยบC Expected PCR product size : 299 bp M N 1 2

300bp

AQUACULTURE EQUINE

Product 48 & 96 Test/kit

299 bp

Fig1. Electrophoresis of PCR product by VetPCRTM EAV Detection Kit Lane M : BrigTM Molecular Weight Marker (Bioingentech Ltda.) Lane N : Negative control Lane 1~3 : EAV Positive sample Lane P : Positive control

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VetPCRTM EEEV Detection Kit

AQUACULTURE EQUINE

DESCRIPTION Venezuelan equine encephalitis (VEE), eastern equine encephalitis (EEE) and western equine encephalitis (WEE) viruses are arthropod borne viruses (arboviruses). They are members of the family Togaviridae, genus Alphavirus. Members of the genus Alphavirus have a spherical, enveloped virion 60 to 65 nm in diameter and possess a single-stranded, positive-sense RNA genome over 11 kb in length. The natural transmission cycles of these three viruses involve a variety of mosquito and avian species. Under unique ecological conditions, VEE, EEE and WEE viruses are transmitted from avian hosts to dead-end hosts such as equines, non-human primates and humans. They are associated with both human and equine encephalitis throughout the Americas. Geographically, there is a difference in the distribution of these three antigenically and ecologically distinct viruses. The majority of EEE virus activity has occurred in the eastern United States within the geographic range of Culiseta melanura, the primary insect vector of North American EEE virus. The majority of WEE virus activity has occurred in the western United States, where Culex tarsalis is the primary mosquito vector of WEE virus. In contrast to both EEE and WEE, the distribution of VEE is primarily restricted to Central and South America. VetPCRTM EEEV Detection Kit is the direct detection of Eastern equine encephalitis virus on the basis of a genetic database, so it can diagnose very fast and accurately. It can amplify only specific gene using the PCR (Polymerase Chain Reaction) method, and take only 4 hours for detection. Therefore, it is a very fast, accurate and reliable technique.

Product

VetPCRTM EEEV Detection Kit

48 & 96 Test/kit

Contents VetPCRTM EEEVRT-PCR Pre-mixture VetPCRTM EEEV PCR Pre-mixture BrigRT-PCRTM solution BiotechTM Transcriptase solution DNase/RNase-free water EEEV RT-PCR Positive control EEEV RT-PCR Positive control Pre-mixture EEEV PCR Positive control Pre-mixture BrigTM Molecular Weight marker Mineral Oil RNA extraction kit (see step 6.1) Characteristics

Kit 48 48 48 1 1 1 1 4 4 1 1 50

Cod. Kit VET0001F(48) or (96) Kit 96 96 96 1 1 1 1 8 8 1 2 100

Ready to use : only DNA template and D.W. are needed Easy and speed protocol Stable for 1 year at -20ยบC Time-saving and cost-effective

Storage Condition Store at -20ยบC Expected PCR product size : 472 bp M

300bp

472 bp

Fig1. Electrophoresis of PCR product by VetPCRTM EEEV Detection Kit Lane M : BrigTM Molecular Weight Marker (Bioingentech Ltda.) Lane 1~3 : EEEV Positive sample Lane P : Positive control Lane N : Negative control

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VetPCRTM EIAV Detection Kit

AQUACULTURE EQUINE

DESCRIPTION Equine infectious anemia (EIA), also known as Swamp Fever, is a viral disease known to occur in all members of Equidae, including horses, mules and donkeys. Equine infectious anemia virus (EIAV) is a member of the genus Lentivirus of the family Retroviridae. EIAV is unique among the lentiviruses in that the initial acute febrile response and associated viremia are followed by recurrent cycles of the disease and, finally, a prolonged asymptomatic period. Acute cases experience fever and hemorrhaging 7-30 days post infection. Very few horses with this initial fever are detected by owners. Chronic infection is marked by episodes of fever, weight loss, depression, progressive weakness, anemia and edema. These signs occur every two weeks in recurring cycles. Other symptoms that may occur during the course of the disease include loss of appetite, frequent urination, diarrhea, weakness, paralysis of the hindquarters, paleness of the mucous membranes, yellowish discoloration of the conjunctiva, small pinpoint hemorrhages beneath the tongue, rapid breathing and accelerated pulse. Pregnant mares may abort. The EIAV most frequently is transmitted between horses in close proximity by large biting insects, such as horseflies and deerflies. VetPCRTM EIAV Detection Kit is the direct detection of Equine infectious anemia virus on the basis of a genetic database, so it can diagnose very fast and accurately. It can amplify only specific gene using the PCR (Polymerase Chain Reaction) method, and take only 4 hours for detection. Therefore, it is a very fast, accurate and reliable technique.

Product

VetPCRTM EIAV Detection Kit

48 & 96 Test/kit

Contents VetPCRTM EIAV RT-PCR Pre-mixture VetPCRTM EIAV PCR Pre-mixture BrigRT-PCRTM solution BiotechTM Transcriptase solution DNase/RNase-free water EIAV RT-PCR Positive control EIAV RT-PCR Positive control Pre-mixture EIAV PCR Positive control Pre-mixture BrigTM Molecular Weight marker Mineral Oil RNA extraction kit (see step 6.1) Characteristics

Kit 48 48 48 1 1 1 1 4 4 1 1 50

Cod. Kit VET0001F(48) or (96) Kit 96 96 96 1 1 1 1 8 8 1 2 100

Ready to use : only DNA template and D.W. are needed Easy and speed protocol Stable for 1 year at -20ยบC Time-saving and cost-effective

Storage Condition Store at -20ยบC Expected PCR product size : 408 bp M

500bp

408 bp

Fig1. Electrophoresis of PCR product by VetPCRTM EIAV Detection Kit Lane M : BrigTM Molecular Weight Marker (Bioingentech Ltda.) Lane 1~4 : EIAV Positive sample Lane P : Positive control Lane N : Negative control

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VetPCRTM EIV-A1 Detection Kit DESCRIPTION Influenza is one of the most important respiratory diseases of the horse. Equine influenza is caused for Equine Influenza Virus type-A (EIV-A), belongs to the Orthomyxovirus family. There are two subtypes of equine influenza virus; A/Equi 1/H7N7 (EIV-A1), first isolated in 1956 and A/Equi 2/ H3N8 (EIV-A2), first isolated in 1963. Both subtypes have caused disease. It is a severe acute upper respiratory infection. Equine influenza appears similar to a range of other viral respiratory diseases. Most of these viruses produce rather mild signs which include a discharge from the nose and coughing. Equine Influenza produces more severe symptoms with horses developing a fever and a dry hacking cough. Horses become ill and are reluctant to eat or drink for several days but usually recover in 2 to 3 weeks. In countries where breeding and racing horses is a major industry, outbreaks of the disease can result in significant economic loss.

AQUACULTURE EQUINE

VetPCRTM EIV-A1 Detection Kit is the direct detection of Equine Influenza Virus type-A1 on the basis of a genetic database, so it can diagnose very fast and accurately. It can amplify only specific gene using the PCR (Polymerase Chain Reaction) method, and take only 4 hours for detection. Therefore, it is a very fast, accurate and reliable technique.

Product

VetPCRTM EIV-A1 Detection Kit

48 & 96 Test/kit

Contents VetPCRTM EIV-A1 RT-PCR Pre-mixture VetPCRTM EIV-A1 PCR Pre-mixture BrigRT-PCRTM solution BiotechTM Transcriptase solution DNase/RNase-free water EIV-A1 RT-PCR Positive control EIV-A1 RT-PCR Positive control Pre-mixture EIV-A1 PCR Positive control Pre-mixture BrigTM Molecular Weight marker Mineral Oil RNA extraction kit (see step 6.1) Characteristics

Kit 48 48 48 1 1 1 1 4 4 1 1 50

Cod. Kit VET0001F(48) or (96) Kit 96 96 96 1 1 1 1 8 8 1 2 100

Ready to use : only DNA template and D.W. are needed Easy and speed protocol Stable for 1 year at -20ยบC Time-saving and cost-effective

Storage Condition Store at -20ยบC Expected PCR product size : 536 bp M

600bp

536 bp

Fig1. Electrophoresis of PCR product by VetPCRTM EIV-A1 Detection Kit Lane M : BrigTM Molecular Weight Marker (Bioingentech Ltda.) Lane N : Negative control Lane 1~3 : EIV-A1 Positive sample Lane P : Positive control

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VetPCRTM EIV-A2 Detection Kit DESCRIPTION Influenza is one of the most important respiratory diseases of the horse. Equine influenza is caused for Equine Influenza Virus type-A (EIV-A), belongs to the Orthomyxovirus family. There are two subtypes of equine influenza virus; A/Equi 1/H7N7 (EIV-A1), first isolated in 1956 and A/Equi 2/ H3N8 (EIV-A2), first isolated in 1963. Both subtypes have caused disease. It is a severe acute upper respiratory infection. Equine influenza appears similar to a range of other viral respiratory diseases. Most of these viruses produce rather mild signs which include a discharge from the nose and coughing. Equine Influenza produces more severe symptoms with horses developing a fever and a dry hacking cough. Horses become ill and are reluctant to eat or drink for several days but usually recover in 2 to 3 weeks. In countries where breeding and racing horses is a major industry, outbreaks of the disease can result in significant economic loss. VetPCRTM EIV-A2 Detection Kit is the direct detection of Equine Influenza Virus type-A2 on the basis of a genetic database, so it can diagnose very fast and accurately. It can amplify only specific gene using the PCR (Polymerase Chain Reaction) method, and take only 4 hours for detection. Therefore, it is a very fast, accurate and reliable technique.

Product

48 & 96 Test/kit

Contents VetPCRTM EIV-A2 RT-PCR Pre-mixture VetPCRTM EIV-A2 PCR Pre-mixture BrigRT-PCRTM solution BiotechTM Transcriptase solution DNase/RNase-free water EIV-A2 RT-PCR Positive control EIV-A2 RT-PCR Positive control Pre-mixture EIV-A2 PCR Positive control Pre-mixture BrigTM Molecular Weight marker Mineral Oil RNA extraction kit (see step 6.1) Characteristics

Kit 48 48 48 1 1 1 1 4 4 1 1 50

Cod. Kit VET0001F(48) or (96) Kit 96 96 96 1 1 1 1 8 8 1 2 100

Ready to use : only DNA template and D.W. are needed Easy and speed protocol Stable for 1 year at -20ยบC Time-saving and cost-effective

Storage Condition Store at -20ยบC Expected PCR product size : 368 bp M

400bp

AQUACULTURE EQUINE

Product

VetPCRTM EIV-A2 Detection Kit

368 bp

Fig1. Electrophoresis of PCR product by VetPCRTM EIV-A2 Detection Kit Lane M : BrigTM Molecular Weight Marker (Bioingentech Ltda.) Lane 1~3 : EIV-A2 Positive sample Lane P : Positive control Lane N : Negative control

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VetPCRTM VEEV Detection Kit

AQUACULTURE EQUINE

DESCRIPTION Venezuelan equine encephalitis (VEE), Eastern equine encephalitis (EEE) and Western equine encephalitis (WEE) viruses are arthropod borne viruses (arboviruses). They are members of the family Togaviridae, genus Alphavirus. Members of the genus Alphavirus have a spherical, enveloped virion 60 to 65 nm in diameter and possess a single-stranded, positivesense RNA genome over 11 kb in length. The natural transmission cycles of these three viruses involve a variety of mosquito and avian species. Under unique ecological conditions, VEE, EEE and WEE viruses are transmitted from avian hosts to dead-end hosts such as equines, non-human primates and humans. They are associated with both human and equine encephalitis throughout the Americas. Geographically, there is a difference in the distribution of these three antigenically and ecologically distinct viruses. The majority of EEE virus activity has occurred in the eastern United States within the geographic range of Culiseta melanura, the primary insect vector of North American EEE virus. The majority of WEE virus activity has occurred in the western United States, where Culex tarsalis is the primary mosquito vector of WEE virus. In contrast to both EEE and WEE, the distribution of VEE is primarily restricted to Central and South America. VetPCRTM VEEV Detection Kit is the direct detection of Venezuelan equine encephalitis virus on the basis of a genetic database, so it can diagnose very fast and accurately. It can amplify only specific gene using the PCR (Polymerase Chain Reaction) method, and take only 4 hours for detection. Therefore, it is a very fast, accurate and reliable technique.

Product

VetPCRTM VEEV Detection Kit

48 & 96 Test/kit

Contents VetPCRTM VEEV RT-PCR Pre-mixture VetPCRTM VEEV PCR Pre-mixture BrigRT-PCRTM solution BiotechTM Transcriptase solution DNase/RNase-free water VEEV RT-PCR Positive control VEEV RT-PCR Positive control Pre-mixture VEEV PCR Positive control Pre-mixture BrigTM Molecular Weight marker Mineral Oil RNA extraction kit (see step 6.1) Characteristics

Kit 48 48 48 1 1 1 1 4 4 1 1 50

Cod. Kit VET0001F(48) or (96) Kit 96 96 96 1 1 1 1 8 8 1 2 100

Ready to use : only DNA template and D.W. are needed Easy and speed protocol Stable for 1 year at -20ยบC Time-saving and cost-effective

Storage Condition Store at -20ยบC Expected PCR product size : 448 bp M

500bp

448 bp

Fig1. Electrophoresis of PCR product by VetPCRTM VEEV Detection Kit Lane M : BrigTM Molecular Weight Marker (Bioingentech Ltda.) Lane N : Negative control Lane P : Positive control Lane 1~4 : VEEV Positive sample

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VetPCRTM WEEV Detection Kit

AQUACULTURE EQUINE

DESCRIPTION Venezuelan equine encephalitis (VEE), Eastern equine encephalitis (EEE) and Western equine encephalitis (WEE) viruses are arthropod borne viruses (arboviruses). They are members of the family Togaviridae, genus Alphavirus. Members of the genus Alphavirus have a spherical, enveloped virion 60 to 65 nm in diameter and possess a single-stranded, positivesense RNA genome over 11 kb in length. The natural transmission cycles of these three viruses involve a variety of mosquito and avian species. Under unique ecological conditions, VEE, EEE and WEE viruses are transmitted from avian hosts to dead-end hosts such as equines, non-human primates and humans. They are associated with both human and equine encephalitis throughout the Americas. Geographically, there is a difference in the distribution of these three antigenically and ecologically distinct viruses. The majority of EEE virus activity has occurred in the eastern United States within the geographic range of Culiseta melanura, the primary insect vector of North American EEE virus. The majority of WEE virus activity has occurred in the western United States, where Culex tarsalis is the primary mosquito vector of WEE virus. In contrast to both EEE and WEE, the distribution of VEE is primarily restricted to Central and South America. VetPCRTM WEEV Detection Kit is the direct detection of Western equine encephalitis virus on the basis of a genetic database, so it can diagnose very fast and accurately. It can amplify only specific gene using the PCR (Polymerase Chain Reaction) method, and take only 4 hours for detection. Therefore, it is a very fast, accurate and reliable technique.

Product

VetPCRTM WEEV Detection Kit

48 & 96 Test/kit

Contents VetPCRTM WEEV RT-PCR Pre-mixture VetPCRTMWEEV PCR Pre-mixture BrigRT-PCRTM solution BiotechTM Transcriptase solution DNase/RNase-free water WEEV RT-PCR Positive control WEEV RT-PCR Positive control Pre-mixture WEEV PCR Positive control Pre-mixture BrigTM Molecular Weight marker Mineral Oil RNA extraction kit (see step 6.1) Characteristics

Kit 48 48 48 1 1 1 1 4 4 1 1 50

Cod. Kit VET0001F(48) or (96) Kit 96 96 96 1 1 1 1 8 8 1 2 100

Ready to use : only DNA template and D.W. are needed Easy and speed protocol Stable for 1 year at -20ยบC Time-saving and cost-effective

Storage Condition Store at -20ยบC Expected PCR product size : 497 bp M

500bp

497 bp

Fig1. Electrophoresis of PCR product by VetPCRTM WEEV Detection Kit Lane M : BrigTM Molecular Weight Marker (Bioingentech Ltda.) Lane N : Negative control Lane P : Positive control Lane 1~4 : WEEV Positive sample

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VetPCRTM WNV Detection Kit DESCRIPTION

AQUACULTURE EQUINE

West Nile virus (WNV) belongs to the genus Flavivirus of the family Flaviviridae and is an arthropod-borne virus. It possesses a singlestranded plus-sense RNA genome of approximately 11,000 nucleotides. This virus can cause encephalitis or meningitis, infection of the brain and the spinal cord. WNV circulates in natural transmission cycles involving primarily Culex species mosquitoes and birds; humans and other mammals, such as primates and horses, are thought to be incidental hosts. Infection with West Nile Virus does not always lead to signs of illness in people or animals. Horses appear to be a species that is susceptible to infection with the virus. In horses that do become clinically ill, the virus infects the central nervous system and may cause symptoms of encephalitis. Clinical signs of encephalitis in horses may include a general loss of appetite and depression, in addition to any combination of the following signs: fever, weakness of hind limbs, paralysis of hind limbs, impaired vision, ataxia, head pressing, aimless wandering, convulsions, inability to swallow, walking in circles, hyperexcitability and coma. VetPCRTM WNV Detection Kit is the direct detection of West Nile virus on the basis of a genetic database, so it can diagnose very fast and accurately. It can amplify only specific gene using the PCR (Polymerase Chain Reaction) method, and take only 4 hours for detection. Therefore, it is a very fast, accurate and reliable technique.

Product

VetPCRTM WNV Detection Kit

48 & 96 Test/kit

Contents VetPCRTM WNV RT-PCR Pre-mixture VetPCRTMWEEV PCR Pre-mixture BrigRT-PCRTM solution BiotechTM Transcriptase solution DNase/RNase-free water WNV RT-PCR Positive control WNV RT-PCR Positive control Pre-mixture WNV PCR Positive control Pre-mixture BrigTM Molecular Weight marker Mineral Oil RNA extraction kit (see step 6.1) Characteristics

Kit 48 48 48 1 1 1 1 4 4 1 1 50

Cod. Kit VET0001F(48) or (96) Kit 96 96 96 1 1 1 1 8 8 1 2 100

Ready to use : only DNA template and D.W. are needed Easy and speed protocol Stable for 1 year at -20ยบC Time-saving and cost-effective

Storage Condition Store at -20ยบC Expected PCR product size : 273 bp M

300bp

273 bp

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VetPCRTM BAR.spp Detection Kit

Product

VetPCR BAR.spp Detection Kit

DESCRIPTION Bartonella spp. are small Gram-negative rods, aerobic and highly fastidious bacteria. They are considered as emergent human pathogens and are the etiological agents for human Cat Scratch Disease (CSD). Recent studies have shown that pet cats serve as a major persistent reservoir host, with prolonged asymptomatic bacteremia, for five Bartonella species: B. henselae, B. clarridgeiae, B. koehlerae, B. weissii, and B. elizabethae. Cat and dog fleas and, less often, deer and dog ticks carry the bacteria and serve as the main vectors for transmission from cat to cat, and they also have the potential to act as vectors in transmitting Bartonella spp. from cats to humans. People who contract the bacteria through a scratch (CSD) develop an erythematous papule at the site of the wound. In 3 to 10 days, lymphadenopathy may develop. People with the disease may also experience fever, anorexia, malaise, headache, myalgia, arthralgia, nausea, and skin eruptions. Most cases of cat scratch disease are self-limiting, but may take several months to resolve.

48 & 96 Test/kit VET0001F(48) or (96)

Contents TM

Kit 48

Kit 96

48 1 1 4 1 1 50

96 1 1 8 1 1 100

Characteristics Ready to use : only DNA template and D.W. are needed Easy and speed protocol Stable for 1 year at -20ยบC Time-saving and cost-effective

Storage Condition Store at -20ยบC Expected PCR product size : 314 bp M

VetPCRTM BAR.spp Detection Kit is the direct detection of Bartonella spp. on the basis of a genetic database, so it can diagnose very fast and accurately. It can amplify only specific gene using the PCR (Polymerase Chain Reaction) method, and take only 3 hours for detection. Therefore, it is a very fast, accurate and reliable technique.

Cod. Kit

400bp

314 bp

FELINE

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VetPCRTM CHLA.FELIS Detection Kit

Product

VetPCR CHLA.FELIS Detection Kit

DESCRIPTION Feline chlamydiosis (also known as feline pneumonitis) is a relatively mild, chronic upper respiratory disease caused by the bacterium Chlamydophila felis (previously Chlamydia psittaci var. felis). The main symptom is conjunctivitis, an abnormal eye discharge due to inflammation of the conjunctiva, the membrane lining the inside of the eyelid. The infection can also cause nasal discharge, sneezing, and pneumonia. Left untreated, the infection tends to become chronic, lasting weeks or months. Chlamydiosis is symptomatic only in the respiratory tract and eyes. When symptoms do appear, the most common include the following: anorexia (loss of appetite; may occur as the disease progresses), coughing, difficulty breathing, fever (may occur as the disease progresses), pneumonia (in young kittens 2 to 4 weeks old, which could be fatal), runny nose (rhinitis), sneezing, watery eyes due to conjunctivitis (either one or both eyes). The inflammation leads to discharge, swelling, and pain; the cats eyes may be red and weepy. This is the main symptom of chlamydiosis and usually occurs initially in one eye and eventually affects both eyes. Rarely, it becomes chronic.

FELINE

VetPCRTM CHLA.FELIS Detection Kit is the direct detection of Chlamydophila felis on the basis of a genetic database, so it can diagnose very fast and accurately. It can amplify only specific gene using the PCR (Polymerase Chain Reaction) method, and take only 3 hours for detection. Therefore, it is a very fast, accurate and reliable technique.

Cod. Kit

48 & 96 Test/kit VET0001F(48) or (96)

Contents TM

VetPCR CHLA.FELIS PCR Pre-mixture DNase/RNase-free water CHLA.FELIS PCR Positive control CHLA.FELIS PCR Positive control Pre-mixture BrigTM Molecular Weight marker Mineral Oil DNA purification kit (see step 6.1)

Kit 48

Kit 96

48 1 1 4 1 1 50

96 1 1 8 1 1 100

Characteristics Ready to use : only DNA template and D.W. are needed Easy and speed protocol Stable for 1 year at -20ยบC Time-saving and cost-effective

Storage Condition Store at -20ยบC Expected PCR product size : 490 bp M

500bp

490 bp

Fig1. Electrophoresis of PCR product by VetPCRTM CHLA.FELIS Detection Kit Lane M : BrigTM Molecular Weight Marker (Bioingentech Ltda.) Lane N : Negative control Lane P :Positive control Lane 1~3 : CHLA.FELIS Positive sample

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VetPCRTM DIR.IM Detection Kit

Product

VetPCR DIR.IM Detection Kit

48 & 96 Test/kit VET0001F(48) or (96)

Contents

DESCRIPTION Feline Heartworm Disease is caused by the parasite Dirofilaria immitis, a worldwide filarial nematode causing heartworm disease in dogs and cats. The life cycle of the parasite involves a definitive host (dog or coyote), mosquitoes, and a susceptible cat. Now in general, the cat is not considered a definitive host for the parasite. Signs of heartworm disease in cats are similar to those in dogs: rapid breathing, coughing, decreased appetite, loss of weight, and listlessness. We may also see vomiting, blindness, and seizures. In some cases, death can occur. On the other hand, some infected cats show no signs at all. Overall, cats are infected by heartworms much less commonly, even in areas in which heavily infected dogs are present. Male cats tend to be infected more commonly and have a larger worm burden than female cats, mostly because male cats spend more time roaming outside and have less resistance to infection than female cat. VetPCRTM DIR.IM Detection Kit is the direct detection of Dirofilaria immitis on the basis of a genetic database, so it can diagnose very fast and accurately. It can amplify only specific gene using the PCR (Polymerase Chain Reaction) method, and take only 3 hours for detection. Therefore, it is a very fast, accurate and reliable technique.

Cod. Kit

VetPCR DIR.IM PCR Pre-mixture DNase/RNase-free water DIR.IM PCR Positive control DIR.IM PCR Positive control Pre-mixture BrigTM Molecular Weight marker Mineral Oil DNA purification kit (see step 6.1)

Kit 48

Kit 96

48 1 1 4 1 1 50

96 1 1 8 1 1 100

Characteristics Ready to use : only DNA template and D.W. are needed Easy and speed protocol Stable for 1 year at -20ยบC Time-saving and cost-effective

Storage Condition Store at -20ยบC Expected PCR product size : 344 bp M

400bp

344 bp

FELINE

Fig1. Electrophoresis of PCR product by VetPCRTM DIR.IM Detection Kit Lane M : BrigTM Molecular Weight Marker (Bioingentech Ltda.) Lane P : Positive control Lane 1~3 : DIR.IM Positive sample Lane N : Negative control

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VetPCRTM FeLV Detection Kit

Product

VetPCR FeLV Detection Kit

DESCRIPTION Feline leukemia virus (FeLV) infection is responsible for more deaths among cats than any other infectious disease. The virus affects domestic cats and occurs in some wild felines as well. There are three main types of feline leukemia virus: FeLV-A, FeLV-B, and FeLV-C. FeLV-positive cats can be infected with one, two, or all three types: FeLV-A occurs in all FeLVinfected cats and causes severeimmunosuppression (weak-ened immune system). FeLV-B occurs in about 50% of all FeLV-infected cats and causes more neoplastic disease (i.e., tumors and other abnormal tissue growths) than cats infected only with FeLV-A. FeLV-C occurs in about 1% of FeLVinfected cats and causes severe anemia. After the initial infection, the virus replicates in the tonsils and pharyngeal lymph nodes (the pharynx is the muscular tube in the neck). Then it spreads via the bloodstream to other parts of the body, especially the lymph nodes, bone marrow, and intestinal tissue, where it continues to replicate. Viremia, the presence of virus in the blood, usually shows up 2 to 4 weeks after the initial infection.

FELINE

VetPCRTM FeLV Detection Kit is the direct detection of Feline leukemia virus on the basis of a genetic database, so it can diagnose very fast and accurately. It can amplify only specific gene using the PCR (Polymerase Chain Reaction) method, and take only 3 hours for detection. Therefore, it is a very fast, accurate and reliable technique.

Cod. Kit

48 & 96 Test/kit VET0001F(48) or (96)

Contents TM

VetPCR FeLV PCR Pre-mixture DNase/RNase-free water FeLV PCR Positive control FeLV PCR Positive control Pre-mixture BrigTM Molecular Weight marker Mineral Oil DNA purification kit (see step 6.1)

Kit 48

Kit 96

48 1 1 4 1 1 50

96 1 1 8 1 1 100

Characteristics Ready to use : only DNA template and D.W. are needed Easy and speed protocol Stable for 1 year at -20ยบC Time-saving and cost-effective

Storage Condition Store at -20ยบC Expected PCR product size : 341 bp M

400bp

Fig1. Electrophoresis of PCR product by VetPCRTM FeLV Detection Kit Lane M : BrigTM Molecular Weight Marker (Bioingentech Ltda.) Lane P :Positive control Lane 1~3 : FeLV Positive sample Lane N : Negative control

341 bp

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VetPCRTM FHV-1 Detection Kit

Product

VetPCR FHV-1 Detection Kit

48 & 96 Test/kit VET0001F(48) or (96)

Contents

DESCRIPTION Feline herpesvirus FHV-1 (previously known as feline rhinotracheitis virus) an Alphaherpesvirus, is one of the most common viruses among cats. FHV-1 causes an acute respiratory illness of cats known as rhinotracheitis. The virus has a worldwide distribution and affects both domestic and wild cats. Infected cats develop respiratory symptoms; sneezing, nasal discharge, rhinitis (inflammation of the nose), and conjunctivitis (inflammation of the membrane lining the eyelid). The virus also affects the reproductive tract and can cause complications during pregnancy. Rhinotracheitis is part of the feline upper respiratory infection complex, which is a group of viral and bacterial infections (e.g., calicivirus, chlamydiosis) that cause sneezing and discharge from the eyes and nose. Cats often have two or more of these upper respiratory infections at the same time, and FHV-1 is one of the most common. VetPCRTM FHV-1 Detection Kit is the direct detection of Feline herpesvirus type-1 on the basis of a genetic database, so it can diagnose very fast and accurately. It can amplify only specific gene using the PCR (Polymerase Chain Reaction) method, and take only 3 hours for detection. Therefore, it is a very fast, accurate and reliable technique.

Cod. Kit

VetPCR FHV-1 PCR Pre-mixture DNase/RNase-free water FHV-1 PCR Positive control FHV-1 PCR Positive control Pre-mixture BrigTM Molecular Weight marker Mineral Oil DNA purification kit (see step 6.1)

Kit 48

Kit 96

48 1 1 4 1 1 50

96 1 1 8 1 1 100

Characteristics Ready to use : only DNA template and D.W. are needed Easy and speed protocol Stable for 1 year at -20ยบC Time-saving and cost-effective

Storage Condition Store at -20ยบC Expected PCR product size : 391 bp M

400bp

391 bp

FELINE

Fig1. Electrophoresis of PCR product by VetPCRTM FHV-1 Detection Kit Lane M : BrigTM Molecular Weight Marker (Bioingentech Ltda.) Lane P : Positive control Lane 1~3 : FHV-1 Positive sample Lane N : Negative control

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VetPCRTM FPV Detection Kit

Product

VetPCR FPV Detection Kit

DESCRIPTION Panleukopenia (feline distemper) is caused by a virus very similar to the one that causes parvovirus disease in dogs. It is very stable in the environment and can survive years at room temperature. It survives well in lower temperatures as well, and is not killed by many of the common disinfectants. Contact with a bleach solution for 10 minutes will inactivate the virus. FPV is most commonly transmitted when a susceptible cat has contact with the feces or urine of infected cats. Infected cats shed the virus in their feces and urine up to 6 weeks after they recover. FPV can also be spread by contact with urine- or feces-contaminated items such as food bowls, water dishes, clothing, shoes, hands, bedding, and litter boxes. The symptoms of panleukopenia can be similar to those seen in dogs with parvo or canine distemper (vomiting, diarrhea, and seizures), which is why the disease is sometimes called ‘feline distemper.’ Panleukopenia in young unvaccinated kittens is usually fatal.

FELINE

48 & 96 Test/kit VET0001F(48) or (96)

Contents TM

VetPCR FPV PCR Pre-mixture DNase/RNase-free water FPV PCR Positive control FPV PCR Positive control Pre-mixture BrigTM Molecular Weight marker Mineral Oil DNA purification kit (see step 6.1)

Kit 48

Kit 96

48 1 1 4 1 1 50

96 1 1 8 1 1 100

Characteristics Ready to use : only DNA template and D.W. are needed Easy and speed protocol Stable for 1 year at -20ºC Time-saving and cost-effective

Storage Condition Store at -20ºC Expected PCR product size : 303 bp M

VetPCRTM FPV Detection Kit is the direct detection of Feline panleukopenia virus on the basis of a genetic database, so it can diagnose very fast and accurately. It can amplify only specific gene using the PCR (Polymerase Chain Reaction) method, and take only 3 hours for detection. Therefore, it is a very fast, accurate and reliable technique.

Cod. Kit

300bp Fig1. Electrophoresis of PCR product by VetPCRTM FPV Detection Kit Lane M : BrigTM Molecular Weight Marker (Bioingentech Ltda.) Lane N : Negative control Lane P :Positive control Lane 1~4 : FPV Positive sample

303 bp

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VetPCRTM MYC.HF Detection Kit

Product

VetPCR MYC.HF Detection Kit

48 & 96 Test/kit VET0001F(48) or (96)

Contents

DESCRIPTION Hemobartonellosis in cats is caused by Mycoplasma haemofelis (Mhf ), a extracellular pleomorphic bacterium previously known as Haemobartonella felis. Cats infected with this organism typically develop regenerative anemia as the bacteria attack the cat’s red blood cells. M. haemofelis can be transmitted from one animal to another by bloodfeeding arthropods such as fleas and ticks. The bacteria can also be transmitted from female cats with clinical disease to their newborn offspring in the absence of arthropod vectors. The severity of disease produced by M. haemofelis varies, from cats that are mildly anemic and without clinical signs to cats that are markedly depressed and die as a result of severe anemia. The most common clinical signs in ill cats are depression, weakness, anorexia, weight loss and pale mucous membranes. Studies have estimated the prevalence of this bacterium in the feline population from 0.9 to 28%. VetPCRTM MYC.HF Detection Kit is the direct detection of Mycoplasma haemofelis on the basis of a genetic database, so it can diagnose very fast and accurately. It can amplify only specific gene using the PCR (Polymerase Chain Reaction) method, and take only 3 hours for detection. Therefore, it is a very fast, accurate and reliable technique.

Cod. Kit

VetPCR MYC.HF PCR Pre-mixture DNase/RNase-free water MYC.HF PCR Positive control MYC.HF PCR Positive control Pre-mixture BrigTM Molecular Weight marker Mineral Oil DNA purification kit (see step 6.1)

Kit 48

Kit 96

48 1 1 4 1 1 50

96 1 1 8 1 1 100

Characteristics Ready to use : only DNA template and D.W. are needed Easy and speed protocol Stable for 1 year at -20ºC Time-saving and cost-effective

Storage Condition Store at -20ºC Expected PCR product size : 410 bp M

400bp

410 bp

FELINE

Fig1. Electrophoresis of PCR product by VetPCRTM MYC.HF Detection Kit Lane M : BrigTM Molecular Weight Marker (Bioingentech Ltda.) Lane N : Negative control Lane P : Positive control Lane 1~4 : MYC.HF Positive sample

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VetPCRTM TOXO.GO Detection Kit

Product

VetPCR TOXO.GO Detection Kit

DESCRIPTION Toxoplasmosis, a disease of cats and other mammalian species, is caused by aparasitic protozoan, Toxoplasma gondii. Protozoa are single-celled organisms that are among the simplest creatures in the animal kingdom. Although infection with Toxoplasma is fairly common, actual disease caused by the parasite is relatively rare. Most cats show no clinical signs of infection with Toxoplasma. Occasionally, however, clinical disease “toxoplasmosis” occurs, kittens and young adult cats being more often affected than older animals. Lethargy, depression, loss of appetite, and fever are typical early nonspecific signs. Pneumonia, manifested by respiratory distress of gradually increasing severity, is the outstanding sign in many cats. Inflammation of the pancreas and enlargement of lymph nodes also occur. Toxoplasmosis can also affect the eyes and central nervous system, producing inflammation of the retina or anterior ocular chamber, abnormal pupil size and responsiveness to light, blindness, incoordination, heightened sensitivity to touch, personality changes, circling, head pressing, twitching of the ears, difficulty in chewing and swallowing food, seizures, and loss of control over urination and defecation.

48 & 96 Test/kit VET0001F(48) or (96)

Contents TM

Kit 48

Kit 96

48 1 1 4 1 1 50

96 1 1 8 1 1 100

Characteristics Ready to use : only DNA template and D.W. are needed Easy and speed protocol Stable for 1 year at -20ºC Time-saving and cost-effective

Storage Condition Store at -20ºC Expected PCR product size : 462 bp M

VetPCR TOXO.GO Detection Kit is the direct detection of Toxoplasma gondii on the basis of a genetic database, so it can diagnose very fast and accurately. It can amplify only specific gene using the PCR (Polymerase Chain Reaction) method, and take only 3 hours for detection. Therefore, it is a very fast, accurate and reliable technique.

Cod. Kit

500bp

462 bp

FELINE

Fig1. Electrophoresis of PCR product by VetPCRTM TOXO.GO Detection Kit Lane M : BrigTM Molecular Weight Marker (Bioingentech Ltda.) Lane P :Positive control Lane 1~3 : TOXO.GO Positive sample Lane N : Negative control

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VetPCRTM FCV Detection Kit DESCRIPTION Feline calicivirus (FCV), member of the family Caliciviridae, genus Vesivirus is a major cause of acute and chronic upper respiratory diseases in cats. Commonly seen clinical signs include rhinitis, conjunctivitis, stomatitis, gingivitis and glossitis. Less often pneumonia, fever, abortion, cystitis and limping are seen. After resolution of the infection, apparently healthy cats may shed virus for several months or even years. Prevalence rates of feline calicivirus in cats without clinical symptoms vary between 2.5 and 25%. These asymptomatic carrier cats are believed to contribute to the epidemiology of feline calicivirus infection and disease. Recently, virulent feline calicivirus strains associated with a hemorrhagic-like fever and mortality up to 60% in adult cats have been described. Feline calicivirus vaccines have been used widely in the field for the last 20 years. Despite these efforts, feline calicivirusrelated illnesses still present a major problem in the feline population especially for cats living in groups. VetPCRTM FCV Detection Kit is the direct detection of Feline calicivirus on the basis of a genetic database, so it can diagnose very fast and accurately. It can amplify only specific gene using the PCR (Polymerase Chain Reaction) method, and take only 4 hours for detection. Therefore, it is a very fast, accurate and reliable technique.

Product VetPCRTM FCV Detection Kit

Product 48 & 96 Test/kit

Contents VetPCRTM FCV RT-PCR Pre-mixture VetPCRTM FCV PCR Pre-mixture BrigRT-PCRTM solution BiotechTM Transcriptase solution DNase/RNase-free water FCV RT-PCR Positive control FCV RT-PCR Positive control Pre-mixture FCV PCR Positive control Pre-mixture BrigTM Molecular Weight marker Mineral Oil RNA extraction kit (see step 6.1) Characteristics

Kit 48 48 48 1 1 1 1 4 4 1 1 50

Cod. Kit VET0001F(48) or (96) Kit 96 96 96 1 1 1 1 8 8 1 2 100

Ready to use : only DNA template and D.W. are needed Easy and speed protocol Stable for 1 year at -20ยบC Time-saving and cost-effective

Storage Condition Store at -20ยบC Expected PCR product size : 309 bp M 1 2 3

300bp

309 bp

FELINE

Fig1. Electrophoresis of PCR product by VetPCRTM FCV Detection Kit Lane M : BrigTM Molecular Weight Marker (Bioingentech Ltda.) Lane 1~3 : FCV Positive sample Lane P : Positive control Lane N : Negative control

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VetPCRTM FIPV Detection Kit DESCRIPTION Feline infectious peritonitis (FIP) is a serious, nearly always fatal systemic viral disease of wild and domestic cats caused by a coronavirus, a type of virus that afflicts pigs, dogs, and in some forms, humans. The clinical name for the virus that causes FIP is feline coronavirus. A common, relatively benign form of feline coronavirus is the feline enteric coronavirus (FECV). When FECV mutates into a disease-causing form, it is called FIPV (feline infectious peritonitis virus). If a cats immune system responds poorly, the mutant FECV may cause the systemic infection called FIP. In wet FIP, fluid accumulates in body cavities-typically in the abdominal cavity, where it causes progressive, but generally painless, swelling; or in the thoracic (chest) cavity, where it causes respiratory distress due to compression of the lungs and fluid backing up into the airways. In dry FIP, fluid buildup is minimal and symptoms depend on which organs are affected by lesions. About half of all dry cases produce eye inflammation or neurological problems: paralysis, unsteady gait, and seizures. Other lesions may cause kidney or liver failure. Weight loss, pancreatic disease, depression, anemia, and fever are usually present. VetPCRTM FIPV Detection Kit is the direct detection of Feline infectious peritonitis virus on the basis of a genetic database, so it can diagnose very fast and accurately. It can amplify only specific gene using the PCR (Polymerase Chain Reaction) method, and take only 4 hours for detection. Therefore, it is a very fast, accurate and reliable technique.

Product VetPCRTM FIPV Detection Kit

Product 48 & 96 Test/kit

Contents VetPCRTM FIPV RT-PCR Pre-mixture VetPCRTM FIPV PCR Pre-mixture BrigRT-PCRTM solution BiotechTM Transcriptase solution DNase/RNase-free water FIPV RT-PCR Positive control FIPV RT-PCR Positive control Pre-mixture FIPV PCR Positive control Pre-mixture BrigTM Molecular Weight marker Mineral Oil RNA extraction kit (see step 6.1) Characteristics

Kit 48 48 48 1 1 1 1 4 4 1 1 50

Cod. Kit VET0001F(48) or (96) Kit 96 96 96 1 1 1 1 8 8 1 2 100

Ready to use : only DNA template and D.W. are needed Easy and speed protocol Stable for 1 year at -20ยบC Time-saving and cost-effective

Storage Condition Store at -20ยบC Expected PCR product size : 587 bp M 1 2 3

600bp

587bp

FELINE

Fig1. Electrophoresis of PCR product by VetPCRTM FIPV Detection Kit Lane M : BrigTM Molecular Weight Marker (Bioingentech Ltda.) Lane 1~3 : FIPV Positive sample Lane P : Positive control Lane N : Negative control

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VetPCRTM FIV Detection Kit DESCRIPTION Feline immunodeficiency virus (FIV) causes an infectious disease in domestic cats and cheetahs similar to human immunodeficiency virus (HIV infection) in humans. It attacks and weakens the body’s immune system, making the animal susceptible to infections and diseases that don’t affect healthy cats. There is neither a cure nor a vaccine for FIV. Though eventually fatal, an FIV-positive cat can live for many years without any signs of illness. FIV is transmitted primarily through deep, penetrating bite wounds. A mother cat may transmit the virus to her newborn kittens during gestation, passage through the birth canal, or nursing. FIV can also be transmitted through the transfusion of contaminated blood. FIV affects only felines. Some of the pathogens (i.e., bacteria, parasites) that cause opportunistic infections in FIV-positive cats may be transmitted from animals to humans and could cause illness in people with compromised immune systems. VetPCRTM FIV Detection Kit is the direct detection of Feline immunodeficiency virus on the basis of a genetic database, so it can diagnose very fast and accurately. It can amplify only specific gene using the PCR (Polymerase Chain Reaction) method, and take only 4 hours for detection. Therefore, it is a very fast, accurate and reliable technique.

Product

VetPCRTM FIV Detection Kit

48 & 96 Test/kit

Contents VetPCRTM FIV RT-PCR Pre-mixture VetPCRTM FIV PCR Pre-mixture BrigRT-PCRTM solution BiotechTM Transcriptase solution DNase/RNase-free water FIV RT-PCR Positive control FIV RT-PCR Positive control Pre-mixture FIV PCR Positive control Pre-mixture BrigTM Molecular Weight marker Mineral Oil RNA extraction kit (see step 6.1) Characteristics

Cod. Kit VET0001F(48) or (96) Kit 96

Kit 48 48 48 1 1 1 1 4 4 1 1 50

96 96 1 1 1 1 8 8 1 2 100

Ready to use : only DNA template and D.W. are needed Easy and speed protocol Stable for 1 year at -20ºC Time-saving and cost-effective

Storage Condition Store at -20ºC Expected PCR product size : 286 bp M N P 1

300bp

286 bp

FELINE

Fig1. Electrophoresis of PCR product by VetPCRTM FIV Detection Kit Lane M : BrigTM Molecular Weight Marker (Bioingentech Ltda. Lane N : Negative control Lane P : Negative control Lane 1~4 : FIV Positive sample

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VetPCRTM ACTI.PLEU Detection Kit

Product

VetPCR ACTI.PLEU Detection Kit

DESCRIPTION Actinobacillus pleuropneumoniae, a gram-negative coccobacillus belonging to the family Pasteurellaceae, is the causative agent of porcine pleuropneumonia, a highly infectious disease of fattening pigs occurring worldwide. This disease is characteristically an acute necrotizing hemorrhagic bronchopneumonia, with accompanying fibrinous pleuritis. A. pleuropneumoniae is carried in the tonsils and upper respiratory tract. It is transmitted short distances by droplet infection and only survives outside the pig for a few days. The organism may affect the pig from weaning through to slaughter but usually the age is from 8 to 16 weeks. The incubation period is very short, as little as 12 hours. Toxins produce severe damage to the lungs. Consequences of infection may range from acute fibrinous pneumonia and pleuritis with high mortality to subclinical infection. VetPCRTM ACTI.PLEU Detection Kit is the direct detection of Actinobacillus pleuropneumoniae on the basis of a genetic database, so it can diagnose very fast and accurately. It can amplify only specific gene using the PCR (Polymerase Chain Reaction) method, and take only 3 hours for detection. Therefore, it is a very fast, accurate and reliable technique.

Cod. Kit

48 & 96 Test/kit VET0001F(48) or (96)

Contents

Kit 48

Kit 96

48 1 1 4 1 1 50

96 1 1 8 1 1 100

VetPCRTM ACTI.PLEU PCR Pre-mixture DNase/RNase-free water ACTI.PLEU PCR Positive control ACTI.PLEU PCR Positive control Pre-mixture BrigTM Molecular Weight marker Mineral Oil DNA purification kit (see step 6.1)

Characteristics Ready to use : only DNA template and D.W. are needed Easy and speed protocol Stable for 1 year at -20ยบC Time-saving and cost-effective

Storage Condition Store at -20ยบC Expected PCR product size : 455 bp M

500bp

455 bp

PORCINE

Fig1. Electrophoresis of PCR product by VetPCRTM ACTI.PLEU Detection Kit Lane M : BrigTM Molecular Weight Marker (Bioingentech Ltda.) Lane 1~3 : ACTI.PLEU Positive sample Lane P : Positive control Lane N : Negative control

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VetPCRTM ADV Detection Kit

Product

VetPCR ADV Detection Kit

DESCRIPTION ADV, also known as pseudorabies virus (PrV, porcine herpesvirus 1), belongs to the family Herpesviridae, subfamily Alphaherpesvirinae. Herpesvirus virions are enveloped, about 150 nm in diameter, and contain an icosahedral nucleocapsid about 100 nm in diameter, composed of 162 capsomers. The genome is linear dsDNA, 125-235 kbp in size. Replication occurs in the nucleus with sequential transcription of immediate early (α), early (β) and late (γ) genes producing α, ©, and γ proteins respectively; the earlier genes and their products regulate the transcription of later genes. DNA replication and encapsidation occur in the nucleus; the envelope is acquired by budding through the inner layer of the nuclear envelope. The virus infects the central nervous system and other organs, such as the respiratory tract, in virtually all mammals except humans and the tailless apes. Aujeszky’s disease (AD) is primarily a disease of swine, which serve as a reservoir and the principal source of natural infection for a diverse range of secondary hosts, including cattle, sheep, goats, dogs, cats, and many feral species. Humans are refractory. VetPCRTM ADV Detection Kit is the direct detection of Aujeszky’s disease virus on the basis of a genetic database, so it can diagnose very fast and accurately. It can amplify only specific gene using the PCR (Polymerase Chain Reaction) method, and take only 3 hours for detection. Therefore, it is a very fast accurate and reliable technique.

Cod. Kit

48 & 96 Test/kit VET0001F(48) or (96)

Contents

Kit 48

Kit 96

48 1 1 4 1 1 50

96 1 1 8 1 1 100

VetPCRTM ADV PCR Pre-mixture DNase/RNase-free water ADV PCR Positive control ADV PCR Positive control Pre-mixture BrigTM Molecular Weight marker Mineral Oil DNA purification kit (see step 6.1)

Characteristics Ready to use : only DNA template and D.W. are needed Easy and speed protocol Stable for 1 year at -20ºC Time-saving and cost-effective

Storage Condition Store at -20ºC Expected PCR product size : 496 bp M

500bp

496 bp

PORCINE

Fig1. Electrophoresis of PCR product by VetPCRTM ADV Detection Kit Lane M : BrigTM Molecular Weight Marker (Bioingentech Ltda.) Lane N : Negative control Lane 1~4 : ADV Positive sample Lane P : Positive control

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VetPCRTM BCHY.HYO Detection Kit

Product

VetPCR BCHY.HYO Detection Kit

DESCRIPTION Brachyspira hyodysenteriae, a Gram-negative, motile, oxygen tolerant, anaerobic, loosely coiled spirochaete; is the etiological agent of swine dysentery (SD), a severe mucohemorrhagic diarrheal disease of weanling to finishing pigs. The disease is usually observed in 15-70 kg pigs, but the disease may also occur in adults and occasionally in suckling piglets. Clinical expression is normally low mortality/high morbidity, and affected pigs can vary from the classical mucohaemorrhagic diarrhoea - various amounts blood and mucus, or faeces varying from soft, grey to light brown to dark. Severe cases can show watery faeces, with blood and mucus, and stained hindquarters. SD is primarily transmitted by ingestion of fecal material either from clinically affected pigs or from clinically normal pigs that carry B. hyodysenteriae. VetPCRTM BCHY.HYO Detection Kit is the direct detection of Brachyspira hyodysenteriae on the basis of a genetic database, so it can diagnose very fast and accurately. It can amplify only specific gene using the PCR (Polymerase Chain Reaction) method, and take only 3 hours for detection. Therefore, it is a very fast, accurate and reliable technique.

Cod. Kit

48 & 96 Test/kit VET0001F(48) or (96)

Contents

Kit 48

Kit 96

48 1 1 4 1 1 50

96 1 1 8 1 1 100

VetPCRTM BCHY.HYO PCR Pre-mixture DNase/RNase-free water BCHY.HYO PCR Positive control BCHY.HYO PCR Positive control Pre-mixture BrigTM Molecular Weight marker Mineral Oil DNA purification kit (see step 6.1)

Characteristics Ready to use : only DNA template and D.W. are needed Easy and speed protocol Stable for 1 year at -20ยบC Time-saving and cost-effective

Storage Condition Store at -20ยบC Expected PCR product size : 446 bp M

500bp

446 bp

PORCINE

Fig1. Electrophoresis of PCR product by VetPCRTM BCHY.HYO Detection Kit Lane M : BrigTM Molecular Weight Marker (Bioingentech Ltda.) Lane P : Positive control Lane 1~4 : BCHY.HYO Positive sample Lane N : Negative control

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VetPCRTM BCHY.PILO Detection Kit

Product

VetPCR BCHY.PILO Detection Kit

DESCRIPTION Brachyspira pilosicoli, the causative agent of porcine intestinal spirochetosis. B. pilosicoli is a weakly beta-hemolytic, anaerobic intestinal spirochete that occurs worldwide in pigs. B. pilosicoli has also been detected in several other farmed and wild animal species, as well as in humans. In growing pigs, the disease causes diarrhoea and affects the production and welfare of animals. The clinical signs of B. pilosicoli infection varied from loose stools to watery, mucoid diarrhoea. VetPCRTM BCHY.PILO Detection Kit is the direct detection of Brachyspira pilosicoli on the basis of a genetic database, so it can diagnose very fast and accurately. It can amplify only specific gene using the PCR (Polymerase Chain Reaction) method, and take only 3 hours for detection. Therefore, it is a very fast, accurate and reliable technique.

Cod. Kit

48 & 96 Test/kit VET0001F(48) or (96)

Contents VetPCRTM BCHY.PILO PCR Pre-mixture DNase/RNase-free water BCHY.PILO PCR Positive control BCHY.PILO PCR Positive control Pre-mixture BrigTM Molecular Weight marker Mineral Oil DNA purification kit (see step 6.1)

Kit 48

Kit 96

48 1 1 4 1 1 50

96 1 1 8 1 1 100

Characteristics Ready to use : only DNA template and D.W. are needed Easy and speed protocol Stable for 1 year at -20ยบC Time-saving and cost-effective

Storage Condition Store at -20ยบC Expected PCR product size : 423 bp M

500bp

423 bp

PORCINE

Fig1. Electrophoresis of PCR product by VetPCRTM BCHY.PILO Detection Kit Lane M : BrigTM Molecular Weight Marker (Bioingentech Ltda.) Lane N : Negative control Lane P : Positive control Lane 1~4 : BCHY.PILO Positive sample

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VetPCRTM BOR.BRON Detection Kit

Product

VetPCR BOR.BRON Detection Kit

DESCRIPTION Bordetella bronchiseptica is a Gram-negative bacterium which usually causes disease in rabbits and wild animals. It is the one of the agents of atrophic rhinitis in swine. Although the organism is generally associated with animal diseases, there have recently been increasing numbers of reports of B. bronchiseptica in immunocompromised human hosts. B. bronchiseptica causes respiratory disease in many species. In particular, it is one of the etiologic agents of atrophic rhinitis and pneumonia in swine. The characteristic lesion of atrophic rhinitis is atrophy of the nasal turbinate bones. Microscopic lesions include mucosal infiltration by neutrophils, loss of cilia, metaplasia of the epithelium, and resorption of bone with replacement by fibrous tissue. Pulmonary lesions are characterized by infiltration of the airways with neutrophils, necrosis of the alveoli and blood vessels, hemorrhage, and, eventually, extensive fibrosis as the lesions become more chronic. VetPCRTM BOR.BRON Detection Kit is the direct detection of Bordetella bronchiseptica on the basis of a genetic database, so it can diagnose very fast and accurately. It can amplify only specific gene using the PCR (Polymerase Chain Reaction) method, and take only 3 hours for detection. Therefore, it is a very fast accurate and reliable technique.

Cod. Kit

48 & 96 Test/kit VET0001F(48) or (96)

Contents

Kit 48

Kit 96

48 1 1 4 1 1 50

96 1 1 8 1 1 100

VetPCRTM BOR.BRON PCR Pre-mixture DNase/RNase-free water BOR.BRON PCR Positive control BOR.BRON PCR Positive control Pre-mixture BrigTM Molecular Weight marker Mineral Oil DNA purification kit (see step 6.1)

Characteristics Ready to use : only DNA template and D.W. are needed Easy and speed protocol Stable for 1 year at -20ยบC Time-saving and cost-effective

Storage Condition Store at -20ยบC Expected PCR product size : 261 bp M

300bp

261 bp

PORCINE

Fig1. Electrophoresis of PCR product by VetPCRTM BOR.BRON Detection Kit Lane M : BrigTM Molecular Weight Marker (Bioingentech Ltda.) Lane P : Positive control Lane 1~4 : BOR.BRON Positive sample Lane N : Negative control

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VetPCRTM HAEM.PARA Detection Kit

Product

VetPCR HAEM.PARA Detection Kit

DESCRIPTION Haemophilus parasuis is a gram-negative bacterium of the family Pasteurellaceae and is the etiologic agent of Glässer’s disease in pigs, which is characterized by serofibrinous to fibrinopurulent polyserositis, arthritis, and meningitis. H. parasuis usually affects young pigs after 4 to 6 weeks in the nursery. Affected animals may show a variety of clinical signs and lesions, depending on the immune status of the herd, virulence of the strain, and stage of infection. Systemic infection by H. parasuis in naive animals is characterized clinically by high fever (41.7 °C), lethargy, swollen joints, coughing, abdominal breathing, and central nervous system signs, such as trembling and paddling. Lesions are characterized by fibrinous exudate on the pericardium, pleura, peritoneum, meninges, and joints. In endemically infected, stable herds, H. parasuis usually affects older animals. VetPCRTM HAEM.PARA Detection Kit is the direct detection of Haemophilus parasuis on the basis of a genetic database, so it can diagnose very fast and accurately. It can amplify only specific gene using the PCR (Polymerase Chain Reaction) method, and take only 3 hours for detection. Therefore, it is a very fast, accurate and reliable technique.

Cod. Kit

48 & 96 Test/kit VET0001F(48) or (96)

Contents VetPCRTM HAEM.PARA PCR Pre-mixture DNase/RNase-free water HAEM.PARA PCR Positive control HAEM.PARA PCR Positive control Pre-mixture BrigTM Molecular Weight marker Mineral Oil DNA purification kit (see step 6.1)

Kit 48

Kit 96

48 1 1 4 1 1 50

96 1 1 8 1 1 100

Characteristics Ready to use : only DNA template and D.W. are needed Easy and speed protocol Stable for 1 year at -20ºC Time-saving and cost-effective

Storage Condition Store at -20ºC Expected PCR product size : 279 bp M

300bp

279 bp

PORCINE

Fig1. Electrophoresis of PCR product by VetPCRTM HAEM.PARA Detection Kit Lane M : BrigTM Molecular Weight Marker (Bioingentech Ltda.) Lane N : Negative control Lane P : Positive control Lane 1~4 : HAEM.PARA Positive sample

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VetPCRTM LAW.INTRA Detection Kit

Product

VetPCR LAW.INTRA Detection Kit

DESCRIPTION Lawsonia intracellularis is the aetiological agent Porcine proliferative enteropathy. It is a Gram negative obligate intracellular bacterium and requires rat enterocyte cell lines for maintenance. The uncomplicated proliferation of mucosa due to L. intracellularis is referred to as Porcine intestinal adenomatosis, while additional changes that may be superimposed on this lesion may result in regional ileitis (muscular hypertrophy), necrotic enteritis (coagulative necrosis) or proliferative haemorrhagic enteropathy (haemorrhage). It is likely that necrotic enteritis represents secondary bacterial infection superimposed on a primary L. intracellularis lesion. VetPCRTM LAW.INTRA Detection Kit is the direct detection of Lawsonia intracellularis on the basis of a genetic database, so it can diagnose very fast and accurately. It can amplify only specific gene using the PCR (Polymerase Chain Reaction) method, and take only 3 hours for detection. Therefore, it is a very fast, accurate and reliable technique.

Cod. Kit

48 & 96 Test/kit VET0001F(48) or (96)

Contents

Kit 48

Kit 96

48 1 1 4 1 1 50

96 1 1 8 1 1 100

VetPCRTM LAW.INTRA PCR Pre-mixture DNase/RNase-free water LAW.INTRA PCR Positive control LAW.INTRA PCR Positive control Pre-mixture BrigTM Molecular Weight marker Mineral Oil DNA purification kit (see step 6.1)

Characteristics Ready to use : only DNA template and D.W. are needed Easy and speed protocol Stable for 1 year at -20ยบC Time-saving and cost-effective

Storage Condition Store at -20ยบC Expected PCR product size : 302 bp M

300bp

302 bp

PORCINE

Fig1. Electrophoresis of PCR product by VetPCRTM LAW.INTRA Detection Kit Lane M : BrigTM Molecular Weight Marker (Bioingentech Ltda.) Lane P : Positive control Lane 1~3 : LAW.INTRA Positive sample Lane N : Negative control

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VetPCRTM LEPT.spp Detection Kit

Product

VetPCR LEPT.spp Detection Kit

DESCRIPTION Leptospirosis is a bacterial disease, caused by various strains of the Leptospira species, a flexible, spiral-shaped, Gram-negative spirochete. Leptospira affects all domestic species including humans. Leptospirosis is spread through the urine of infected animals, which gets into water or soil and can survive there for weeks to months. Humans and animals are most likely to get leptospirosis from drinking water contaminated with the bacteria.Human disease may range from mild to severe. Signs include flu-like illness (fever, body aches, headache), weakness, vomiting, mental confusion, jaundice (orange/yellow skin color), and stiff neck. Severe cases can lead to damage of the liver, kidney or central nervous system (brain, spinal cord). L. pomona is the most common serovar affecting pigs; less commonly L. tarassovi infection may occur. The signs seen are as follows: abortion, stillbirths or the birth of live, weak piglets; kidney lesions (white spots) in grower/finisher pigs; rarely fever, jaundice and death may occur in young piglets. VetPCRTM LEPT spp. Detection Kit is the direct detection of Leptospira spp. on the basis of a genetic database, so it can diagnose very fast and accurately. It can amplify only specific gene using the PCR (Polymerase Chain Reaction) method, and take only 3 hours for detection. Therefore, it is a very fast, accurate and reliable technique.

Cod. Kit

48 & 96 Test/kit VET0001F(48) or (96)

Contents

Kit 48

Kit 96

48 1 1 4 1 1 50

96 1 1 8 1 1 100

VetPCRTM LEPT.spp PCR Pre-mixture DNase/RNase-free water LEPT.spp PCR Positive control LEPT.spp PCR Positive control Pre-mixture BrigTM Molecular Weight marker Mineral Oil DNA purification kit (see step 6.1)

Characteristics Ready to use : only DNA template and D.W. are needed Easy and speed protocol Stable for 1 year at -20ยบC Time-saving and cost-effective

Storage Condition Store at -20ยบC Expected PCR product size : 348 bp M

400bp

348 bp

PORCINE

Fig1. Electrophoresis of PCR product by VetPCRTM LEPT.spp Detection Kit Lane M : BrigTM Molecular Weight Marker (Bioingentech Ltda.) Lane N : Negative control Lane P : Positive control Lane 1~3 : LEPT.spp Positive sample

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VetPCRTM LISTE.MON Detection Kit

Product

VetPCR LISTE.MON Detection Kit

DESCRIPTION Listeriosis usually results from infection by Listeria monocytogenes, a Gram positive rod in the family Listeriaceae. This organism is a facultative intracellular pathogen and cause animal and human disease. Listeriosis is usually a serious problem only in pregnant women, newborns, the elderly, and immunocompromised or debilitated hosts. Most human infections are caused by eating the bacteria in food, but the bacteria can also be spread by inhalation or direct contact. Most cases in the United States involve newborns. Women can become infected during pregnancy but usually show no signs of illness, however their fetus or newborn infant can die from the infection. The elderly or individuals with weak immune systems are also at greater risk for the disease. A skin infection form of the disease can occur in people who handle sick animals. In pigs, exposure results in infection but disease is uncommon. In piglets and weaners the bacterium may cause; a septicaemia and high temperature in piglets, nervous signs possibly meningitis, weak piglets at birth, pneumonia, head on one side, middle ear infections. VetPCRTM LISTE.MON Detection Kit is the direct detection of Listeria monocytogenes on the basis of a genetic database, so it can diagnose very fast and accurately. It can amplify only specific gene using the PCR (Polymerase Chain Reaction) method, and take only 3 hours for detection. Therefore, it is a very fast, accurate and reliable technique.

Cod. Kit

48 & 96 Test/kit VET0001F(48) or (96)

Contents

Kit 48

Kit 96

48 1 1 4 1 1 50

96 1 1 8 1 1 100

VetPCRTM LISTE.MON PCR Pre-mixture DNase/RNase-free water LISTE.MON PCR Positive control LISTE.MON PCR Positive control Pre-mixture BrigTM Molecular Weight marker Mineral Oil DNA purification kit (see step 6.1)

Characteristics Ready to use : only DNA template and D.W. are needed Easy and speed protocol Stable for 1 year at -20ยบC Time-saving and cost-effective

Storage Condition Store at -20ยบC Expected PCR product size : 322 bp M

400bp

322 bp

PORCINE

Fig1. Electrophoresis of PCR product by VetPCRTM LISTE.MON Detection Kit Lane M : BrigTM Molecular Weight Marker (Bioingentech Ltda.) Lane N : Negative control Lane P : Positive control Lane 1~4 : LISTE.MON Positive sample

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VetPCRTM MYCO.HYOP Detection Kit

Product

VetPCR MYCO.HYOP Detection Kit

DESCRIPTION Mycoplasma hyopneumoniae is the causative agent of enzootic pneumonia and plays an important role in the porcine respiratory disease complex (PRDC). The chronic form of the disease affects up to 80% of pigs around the world and is characterized by high morbidity and low mortality rates. As a single infection, it causes enzootic pneumonia, a prevalent and persistent respiratory disease of swine. In combination with other respiratory pathogens, i.e., porcine reproductive and respiratory syndrome virus (PRRSV) or swine influenza virus, however, pneumonia due to the second pathogen is significantly enhanced. Vaccination against M. hyopneumoniae does not prevent its colonization of the swine respiratory tract or protect sufficiently against disease, nor does it obviate the potentiating role of M. hyopnuemoniae in dual infection with other infectious pathogens. VetPCRTM MYCO.HYOP Detection Kit is the direct detection of Mycoplasma hyopneumoniae on the basis of a genetic database, so it can diagnose very fast and accurately. It can amplify only specific gene using the PCR (Polymerase Chain Reaction) method, and take only 3 hours for detection. Therefore, it is a very fast accurate and reliable technique.

Cod. Kit

48 & 96 Test/kit VET0001F(48) or (96)

Contents VetPCRTM MYCO.HYOP PCR Pre-mixture DNase/RNase-free water MYCO.HYOP PCR Positive control MYCO.HYOP PCR Positive control Pre-mixture BrigTM Molecular Weight marker Mineral Oil DNA purification kit (see step 6.1)

Kit 48

Kit 96

48 1 1 4 1 1 50

96 1 1 8 1 1 100

Characteristics Ready to use : only DNA template and D.W. are needed Easy and speed protocol Stable for 1 year at -20ยบC Time-saving and cost-effective

Storage Condition Store at -20ยบC Expected PCR product size : 596 bp M

600bp

596 bp

PORCINE

Fig1. Electrophoresis of PCR product by VetPCRTM MYCO.HYOP Detection Kit Lane M : BrigTM Molecular Weight Marker (Bioingentech Ltda.) Lane 1~3 : MYCO.HYOP Positive sample Lane P : Positive control Lane N : Negative control

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VetPCRTM PAS.MUL Detection Kit

Product

VetPCR PAS.MUL Detection Kit

DESCRIPTION Pasteurella multocida is a Gram-negative, nonmotile, facultatively anaerobic coccobacillus that forms part of the commensal flora in the oral cavity, upper respiratory and gastrointestinal tract of domesticated and wild animals. However, it is capable of producing septicemic or respiratory diseases in animals by infecting organs and tissues that have been previously weakened by stress, injuries or other microbial infections. In addition, this bacterium also acts as an opportunistic pathogen to humans, since bite and scratch wounds from pet animals such as cats and dogs can result in severe localized infections. VetPCRTM PAS.MUL Detection Kit is the direct detection of Pasteurella multocida on the basis of a genetic database, so it can diagnose very fast and accurately. It can amplify only specific gene using the PCR (Polymerase Chain Reaction) method, and take only 3hours for detection. Therefore, it is a very fast accurate and reliable technique.

Cod. Kit

48 & 96 Test/kit VET0001F(48) or (96)

Contents

Kit 48

Kit 96

48 1 1 4 1 1 50

96 1 1 8 1 1 100

VetPCRTM PAS.MUL PCR Pre-mixture DNase/RNase-free water PAS.MUL PCR Positive control PAS.MUL PCR Positive control Pre-mixture BrigTM Molecular Weight marker Mineral Oil DNA purification kit (see step 6.1)

Characteristics Ready to use : only DNA template and D.W. are needed Easy and speed protocol Stable for 1 year at -20ยบC Time-saving and cost-effective

Storage Condition Store at -20ยบC Expected PCR product size : 252 bp M

300bp

252 bp

PORCINE

Fig1. Electrophoresis of PCR product by VetPCRTM PAS.MUL Detection Kit Lane M : BrigTM Molecular Weight Marker (Bioingentech Ltda.) Lane P : Positive control Lane 1~4 : PAS.MUL Positive sample Lane N : Negative control

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VetPCRTM PCV-2 Detection Kit

Product

VetPCR PCV-2 Detection Kit

DESCRIPTION Postweaning multisystemic wasting syndrome (PMWS) was initially recognized in weaning piglets of a high-health-status herd. Since then, PMWS has become an economically important disease in virtually all regions of the world that produce pigs. PMWS primarily affects pigs between 5 and 18 weeks of age. Clinical PMWS signs include progressive weight loss, dyspnea, tachypnea, anemia, diarrhea, and jaundice. The primary causative agent of PMWS has been identified as porcine circovirus type-2 (PCV2). Porcine circovirus type-1 (PCV-1) was discovered as a noncytopathic contaminant of the porcine kidney cell line PK-15 and is nonpathogenic to pigs. Both PCV-1 and PCV-2 are small, nonenveloped viruses with a single-stranded circular DNA genome of about 1.76 kb. PCV belongs to the Circoviridae family along with chicken anemia virus, psittacine beak and feather disease virus, and tentative members columbid circovirus, goose circovirus, and canary circovirus. VetPCRTM PCV-2 Detection Kit is the direct detection of Porcine circovirus type-2 on the basis of a genetic database, so it can diagnose very fast and accurately. It can amplify only specific gene using the PCR (Polymerase Chain Reaction) method, and take only 3 hours for detection. Therefore, it is a very fast accurate and reliable technique.

Cod. Kit

48 & 96 Test/kit VET0001F(48) or (96)

Contents VetPCRTM PCV-2 PCR Pre-mixture DNase/RNase-free water PCV-2 PCR Positive control PCV-2 PCR Positive control Pre-mixture BrigTM Molecular Weight marker Mineral Oil DNA purification kit (see step 6.1)

Kit 48

Kit 96

48 1 1 4 1 1 50

96 1 1 8 1 1 100

Characteristics Ready to use : only DNA template and D.W. are needed Easy and speed protocol Stable for 1 year at -20ยบC Time-saving and cost-effective

Storage Condition Store at -20ยบC Expected PCR product size : 462 bp M

500bp

462 bp

PORCINE

Fig1. Electrophoresis of PCR product by VetPCRTM PCV-2 Detection Kit Lane M : BrigTM Molecular Weight Marker (Bioingentech Ltda.) Lane N : Negative control Lane P : Positive control Lane 1~3 : PCV-2 Positive sample

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VetPCRTM SALMO.spp Detection Kit

Product

VetPCR SALMO.spp Detection Kit

DESCRIPTION Salmonella are facultative anaerobic, non-spore forming, Gram-negative rods of the family Enterobacteriaceae. Enteric salmonellosis is characterized by an acute colitis that results in profuse diarrhea and, occasionally, abdominal pain. Animals become infected with Salmonella through a fecal-oral route of transmission. This occurs by eating materials (e.g., feed, water, pasture grass) contaminated with feces of an infected animal. The bacteria can live for months to years in warm, wet environments. Signs can include watery, foul smelling diarrhea, abdominal pain and fever. Dehydration and shock can occur and lead to death; this is most common in young animals. Salmonella multiply mainly in the intestines of young growing pigs but also in some sows. They may be shed in faeces for several weeks or months with no clinical disease. Salmonella in the gut of the pig can contaminate carcasses during the slaughter process and their presence creates potential public health risks from food poisoning. VetPCRTM SALMO spp. Detection Kit is the direct detection of Salmonella spp. on the basis of a genetic database, so it can diagnose very fast and accurately. It can amplify only specific gene using the PCR (Polymerase Chain Reaction) method, and take only 3 hours for detection. Therefore, it is a very fast, accurate and reliable technique.

Cod. Kit

48 & 96 Test/kit VET0001F(48) or (96)

Contents

Kit 48

Kit 96

48 1 1 4 1 1 50

96 1 1 8 1 1 100

VetPCRTM SALMO.spp PCR Pre-mixture DNase/RNase-free water SALMO.spp PCR Positive control SALMO.spp PCR Positive control Pre-mixture BrigTM Molecular Weight marker Mineral Oil DNA purification kit (see step 6.1)

Characteristics Ready to use : only DNA template and D.W. are needed Easy and speed protocol Stable for 1 year at -20ยบC Time-saving and cost-effective

Storage Condition Store at -20ยบC Expected PCR product size : 319 bp M

400bp

319 bp

PORCINE

Fig1. Electrophoresis of PCR product by VetPCRTM SALMO.spp Detection Kit Lane M : BrigTM Molecular Weight Marker (Bioingentech Ltda.) Lane P : Positive control Lane 1~3 : SALMO.spp Positive sample Lane N : Negative control

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VetPCRTM PoRV Detection Kit DESCRIPTION Porcine rubulavirus (PoRV) infection or “blue eye” disease is caused by the porcine rubulavirus, which is also called the La-Piedad-Michoacan paramyxovirus (LPMV). This virus, which was first isolated in Mexico in the early 1980s, belongs to the genus Rubulavirus and family Paramyxoviridae. It is characterized by encephalitis and respiratory disease in piglets, reproductive failure in adult pigs, and occasional corneal opacity in all ages. The clinical signs observed are variable and dependant on the age of the pigs infected. Typically, the disease in piglets 2-15 days of age usually start with fever, a rough hair coat, and an arched back. These signs are quickly followed by progressive nervous signs of ataxia, weakness, rigidity, muscle tremor, abnormal posture, and death within 48 hours. In older pigs, clinical signs are much less severe, consisting of transient fever, sneezing, and coughing. Nervous system signs were less common and less obvious, and mortality rates are very low. VetPCRTM PoRV Detection Kit is the direct detection of Porcine rubulavirus on the basis of a genetic database, so it can diagnose very fast and accurately. It can amplify only specific gene using the PCR (Polymerase Chain Reaction) method, and take only 3 hours for detection. Therefore, it is a very fast, accurate and reliable technique.

Product

VetPCRTM PoRV Detection Kit

48 & 96 Test/kit

Contents VetPCRTM PoRV RT-PCR Pre-mixture VetPCRTM PoRV PCR Pre-mixture BrigRT-PCRTM solution BiotechTM Transcriptase solution DNase/RNase-free water PoRV RT-PCR Positive control PoRV RT-PCR Positive control Pre-mixture PoRV PCR Positive control Pre-mixture BrigTM Molecular Weight marker Mineral Oil RNA extraction kit (see step 6.1) Characteristics

Kit 48 48 48 1 1 1 1 4 4 1 1 50

Cod. Kit VET0001F(48) or (96) Kit 96 96 96 1 1 1 1 8 8 1 2 100

Ready to use : only DNA template and D.W. are needed Easy and speed protocol Stable for 1 year at -20ºC Time-saving and cost-effective

Storage Condition Store at -20ºC Expected PCR product size : 432 bp M

500bp

432 bp

PORCINE

Fig1. Electrophoresis of PCR product by VetPCRTM PoRV Detection Kit Lane M : BrigTM Molecular Weight Marker (Bioingentech Ltda.) Lane 1~4 : PoRV Positive sample Lane P : Positive control Lane N : Negative control

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VetPCRTM PRRSV Detection Kit DESCRIPTION The etiologic agent is a virus in the group Arteriviridae. The virus is enveloped and ranges in size from 45 to 80 mm. Inactivation is possible after treatment with ether or chloroform; however, the virus is very stable under freezing conditions, retaining its infectivity for 4 mo at -70 C. As the temperature rises, infectivity is reduced (15-20 min at 56 C). PRRS appears to have two distinct clinical phases: reproductive failure and postweaning respiratory diseases. The reproductive phase of the disease includes increases in the number of stillborn piglets, mummified fetuses, premature farrowings, and weak-born pigs. Stillbirths and mummies may increase up to 25-35%, and abortions can exceed 10%. Anorexia and agalactia are evident in lactating sows and result in increased (30-50%) preweaning mortality. Suckling piglets develop a characteristic thumping respiratory pattern, and histopathologic examination of lung tissue reveals a severe, necrotizing, interstitial pneumonia. VetPCRTM PRRSV Detection Kit is the direct detection of Porcine reproductive and respiratory syndrome virus on the basis of a genetic database, so it can diagnose very fast and accurately. It can amplify only specific gene using the PCR (Polymerase Chain Reaction) method, and take only 3 hours for detection. Therefore, it is a very fast accurate and reliable technique.

Product VetPCRTM PRRSV Detection Kit

Product 48 & 96 Test/kit

Contents VetPCRTM PRRSV RT-PCR Pre-mixture VetPCRTM PRRSV PCR Pre-mixture BrigRT-PCRTM solution BiotechTM Transcriptase solution DNase/RNase-free water PRRSV RT-PCR Positive control PRRSV RT-PCR Positive control Pre-mixture PRRSV PCR Positive control Pre-mixture BrigTM Molecular Weight marker Mineral Oil RNA extraction kit (see step 6.1) Characteristics

Kit 48 48 48 1 1 1 1 4 4 1 1 50

Cod. Kit VET0001F(48) or (96) Kit 96 96 96 1 1 1 1 8 8 1 2 100

Ready to use : only DNA template and D.W. are needed Easy and speed protocol Stable for 1 year at -20ยบC Time-saving and cost-effective

Storage Condition Store at -20ยบC Expected PCR product size : 496 bp M 1 2 3

500bp

496 bp

PORCINE

Fig1. Electrophoresis of PCR product by VetPCRTM PRRSV Detection Kit Lane M : BrigTM Molecular Weight Marker (Bioingentech Ltda.) Lane 1~3 : PRRSV Positive sample Lane P : Positive control Lane N : Negative control

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VetPCRTM SIV Detection Kit DESCRIPTION Swine influenza is an acute, highly contagious, respiratory disease that results from infection with type A influenza virus. It is commonly known in the industry as Swine Flu, Hog Flu, and Pig Flu. The etiology of Hog Flu is a Type A Influenza virus which belongs to the Orthomyxoviridae family. It is an enveloped, RNA virus with a lipid envelope and a helical nucleocapsid. Field isolates of variable virulence exist, and clinical manifestation may be determined by secondary organisms. Pigs are the principal hosts of classic swine influenza virus. Influenza is transmitted primarily pig-to-pig by the nasopharyngeal route. Nasal secretions are laden with virus during the acute febrile stage. The virus is easily carried and spread by avian species, particularly waterfowl, and humans. Care should be taken to prevent spread from and between birds and humans to swine. The virus can be shed for 30 days post-inoculation and has been recovered from clinically normal animals. VetPCRTM SIV Detection Kit is the direct detection of Swine influenza virus on the basis of a genetic database, so it can diagnose very fast and accurately. It can amplify only specific gene using the PCR (Polymerase Chain Reaction) method, and take only 3 hours for detection. Therefore, it is a very fast accurate and reliable technique.

Product VetPCRTM SIV Detection Kit

Product 48 & 96 Test/kit

Contents VetPCRTM SIV RT-PCR Pre-mixture VetPCRTM SIV PCR Pre-mixture BrigRT-PCRTM solution BiotechTM Transcriptase solution DNase/RNase-free water SIV RT-PCR Positive control SIV RT-PCR Positive control Pre-mixture SIV PCR Positive control Pre-mixture BrigTM Molecular Weight marker Mineral Oil RNA extraction kit (see step 6.1) Characteristics

Cod. Kit VET0001F(48) or (96) Kit 96

Kit 48 48 48 1 1 1 1 4 4 1 1 50

96 96 1 1 1 1 8 8 1 2 100

Ready to use : only DNA template and D.W. are needed Easy and speed protocol Stable for 1 year at -20ยบC Time-saving and cost-effective

Storage Condition Store at -20ยบC Expected PCR product size : 582 bp M N 1 2

600bp

582 bp

PORCINE

Fig1. Electrophoresis of PCR product by VetPCRTM SIV Detection Kit Lane M : BrigTM Molecular Weight Marker (Bioingentech Ltda.) Lane N : Negative control Lane 1~3 : SIV Positive sample Lane P : Positive control

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