第一次重複實驗整理 為確認結果之再現性以及我們發現的正確性,本論文在12/17受pubpeer質疑後即開始 三次獨立重複實驗,無論是否有被誤解均一律重作,已於2/17完成三重複實驗,完全驗 證先前之發現,茲將三次重複獨立實驗之整理結果以及原始資料呈現如下。
1B (right panel) OECM‐1‐ sh‐scr
FaDu‐ CDH
1 2
BMI1
FaDu‐BMI1 EV let‐7i
β ‐Actin
Aurora A
Aurora A
p53
β ‐Actin
β ‐Actin
SCR OECM1‐sh‐scr Bi1 OECM1‐sh‐BMI1‐1 Bi2 OECM1‐sh‐BMI1‐2
1.5
FaDu –BMI1 FaDu ‐ CDH sh‐scr sh‐AURKA
BMI1
BMI1
Aurora A
Fold change of protein level
4C
3I
OECM‐1‐ sh‐BMI1
MG132 phospho‐p53 (ser315)
1
+ + +
β ‐Actin 0.5 0
BMI1 BMI1
5D
Aurora A AURKA
5G
FaDu‐ FaDu –BMI1 CDH sh‐scr sh‐AURKA
Snail
FaDu – BMI1 FaDu ‐ CDH sh‐scr sh‐AURKA
SAS‐ sh‐scr BMI1
BMI1
Phospho‐AKT (ser 473)
Aurora A
Total AKT
Phospho‐GSK‐3β (ser9)
Phospho‐GSK3β (ser9)
Total GSK‐3β
Total GSK3β
BMI1 Aurora A
S2C
β ‐Actin
β ‐catenin
β‐Actin
Aurora A
S3E OECM‐1‐ sh‐scr
OECM‐1‐ sh‐AURKA 1 2
S3F OECM‐1‐ sh‐scr
BMI1
BMI1
Aurora A
Aurora A
p53
p53
β ‐actin
β ‐actin
MG132 phospho‐p53 (ser315) β ‐actin
+ + +
MG132 phospho‐p53 (ser315) β ‐actin
OECM‐1‐ sh‐BMI1 1 2
+ + +
β ‐actin
SAS‐ sh‐BMI1 1 2