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回覆科技部(實驗原始資料與重複驗證整理)

Page 5

第一次重複實驗整理 為確認結果之再現性以及我們發現的正確性,本論文在12/17受pubpeer質疑後即開始 三次獨立重複實驗,無論是否有被誤解均一律重作,已於2/17完成三重複實驗,完全驗 證先前之發現,茲將三次重複獨立實驗之整理結果以及原始資料呈現如下。

1B (right panel) OECM‐1‐ sh‐scr

FaDu‐ CDH

1 2

BMI1

FaDu‐BMI1 EV let‐7i

β ‐Actin

Aurora A

Aurora A

p53

β ‐Actin

β ‐Actin

SCR OECM1‐sh‐scr Bi1 OECM1‐sh‐BMI1‐1 Bi2 OECM1‐sh‐BMI1‐2

1.5

FaDu –BMI1 FaDu ‐ CDH sh‐scr sh‐AURKA

BMI1

BMI1

Aurora A

Fold change of protein level

4C

3I

OECM‐1‐ sh‐BMI1

MG132 phospho‐p53 (ser315)

1

+ + +

β ‐Actin 0.5 0

BMI1 BMI1

5D

Aurora A AURKA

5G

FaDu‐ FaDu –BMI1 CDH sh‐scr sh‐AURKA

Snail

FaDu – BMI1 FaDu ‐ CDH sh‐scr sh‐AURKA

SAS‐ sh‐scr BMI1

BMI1

Phospho‐AKT (ser 473)

Aurora A

Total AKT

Phospho‐GSK‐3β (ser9)

Phospho‐GSK3β (ser9)

Total GSK‐3β

Total GSK3β

BMI1 Aurora A

S2C

β ‐Actin

β ‐catenin

β‐Actin

Aurora A

S3E OECM‐1‐ sh‐scr

OECM‐1‐ sh‐AURKA 1 2

S3F OECM‐1‐ sh‐scr

BMI1

BMI1

Aurora A

Aurora A

p53

p53

β ‐actin

β ‐actin

MG132 phospho‐p53 (ser315) β ‐actin

+ + +

MG132 phospho‐p53 (ser315) β ‐actin

OECM‐1‐ sh‐BMI1 1 2

+ + +

β ‐actin

SAS‐ sh‐BMI1 1 2


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