Molecular Biology | XL Biotec

MOLECULAR BIOLOGY

Blue Gel Loading Buffer

BLUE GEL LOADING BUFFER, GEL CHROMATOGRAPHY – JENA BIOSCIENCE

Blue Gel Loading Buffer

Gel loading for Jena Bioscience In order to make loading DNA-containing samples into the wells of agarose and polyacrylamide gels easier, buffers have been developed.

TracBioscienceking dyes are present in the buffers as a DNA fragment migration indication. They also include glycerol to increase density and EDTA to stop nuclease activity.

Total RNA Purification Kit The Total RNA Purification Kit is made to quickly, efficiently, and effectively separate total RNA from small amounts of different samples such blood, plant and animal tissue, bacteria, and viruses.

Complete removal of inhibitors, such as divalent cations and proteins, is possible with the spin column-based technique. The absence of phenol makes handling the kit safe and ensures that no dangerous waste is generated. Numerous downstream applications might make use of the whole RNA that has been isolated. Up to 100 g of RNA can be purified using the kit for each batch.

Preparation procedure: Before start, add the following components (not included in the kit) as indicated on the respective bottle: 2-Mercaptoethanol to the Lysis Buffer (10 μl 2Mercaptoethanol per 1 ml Lysis Buffer) 96-99 % Ethanol to Blood Washing Buffer, First Washing Buffer and Second Washing Buffer

Haemophilus

In newborns and young children, Haemophilus influenzae type b can cause bacteremia, pneumonia, and acute bacterial meningitis. To prevent infection, vaccines made of a protein-conjugated polysaccharide from Haemophilus influenzae type b have been created. Fundamentals of the Assay When test specimens, which may contain Human IgG reactive antibodies, are subjected to microtiter wells coated with Haemophilus influenzae type b oligosaccharide conjugated to human serum abumin (HbOHA), the results are recorded. Unbound components in the test sample are washed away following an incubation period. During a second incubation phase, specific bound Human IgG react with an anti-Human IgG conjugated with HRP. A specific bound enzyme conjugate is identified after a second wash cycle by its interaction with the substrate solution, 2,2′ Azino-di[3-ethyl-benzthiazolinesulfonate (ABTS). The assay is spectrophotometrically analyzed to determine the quantity of a sample contains human anti-Haemophilus influenzae type B antibodies.

Deoxyribonuclease I Enzyme

Deoxyribonuclease I Enzyme (Molecular Biology Grade) Mybiosource,USA Deoxyribonuclease I (DNase I), >=1500u/mg, Lyophilized; EC=3.1.21.1; 5′Oligonucleotidohydrolase Product Synonym Names Deoxyribonuclease I (DNase I), 1500u/mg, Lyophilized Research Use Only For Research Use Only. Not for use in diagnostic procedures.

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