peimand -15

.n eperaion meere e l-dlonine, Glycine & l- ueie uang n-Oukone tehe acid nWalen in ralio (9 12 0).

2. apBeDMMÉA oromagraahy papn ship, choanahgrophic gr, g/oan ofpenoil, scale, ninhydrin reagent spry, copitbry Rioe

. Prinuale 9he given experimer 6o7d an min cpte o diPAent raki ob a bo opHon o di¢Ferrnt componenh prent in mixke coluhan, Dhe Componen wih vigher ataopon rak will pam to Aigher lw es ond urte verte, hen Hhe 8olvent an caled mobile phae while chromahog rophy pap A ttakonay phae. he dFfan /euet o ditAnn companenh Cen bo hon Seen óy 6prying ninhyonin reagent, vhich reorh uih amino avd do yelo purple Co/oumd e'pes

4.oedske io) Cre,pon feak mirkne 6y dibroluing 5mg ob eoch o omino atd in s0 ml. o walen. prepore developvr solulion 6 oml. n- bukanol , 1o ml. glociat oroHe qcid ond 5om). o waer, puk ib in chroma/o9uphy Jr. () pp0e 0.9 Pol. e ninhyhin uoing abaoluk aleohol.

Teacher's Remarks.

Teacher's Sigrature.

Experiment No.5. Date Page No. 52_

) Drouw 0 line nean one end ek he Qhromaogrephy Poper, ca * O00/ine ohow onokhen 1ine ?em, adove he bare tine poink the cenhe o6 d line by morking (X).

5 By the help og apilloy tube puf one odrop o P<pned tAt mixkure af "x" pnion.

(6) puk he ohroma/agrophy paper ship in chromd a9Taphy Jar Coneining 1:5 of douelopor sohuton, such hat he deuelo -per Solukon d'oes' reoch he doint "x '" manked in chramalo09 a/ph pupn, bong +he poper Ar 5 houm so, dewelopur Coluion muy gpead an d rite.

()Remoue he 8hine, ory i (les it dry nak1rally ), 4hon enay the shipe cwih Dinh ydin soluhon.

8 Meanun fAe dis/ance 4rauelted by diPrxnt companen k Fom bae line as we o soluent, than caleuak he Retardahjon Pa chr o Rr ualue

Gbsernkong- 9o oaleudak Ro uatue R: is4gnte travele d 6y comoanenhr Disbone raveled éy solsen^ (o'euelppen)

Teacher's Signature eacher's Remarks.

.OomponehtA-

R: d 096 e. Componen A is "Gyine " . Componenf ARE G. 0.0 i.e, Componen B i "dl-Alonine " 3, Componenk e 9. 0.61 e, Component is (-/ucine

6, scelta he rale avtarpion of t-(ucine i ower". Abon d,l.Abnine ond Gy cine , he order o6 odcophion oj 8 amino coeid in deue foper coluhon f n-6utenel, Arekie auid and waer C:115) con be wikn ao Gulycine > dl-A/anine fucine

Teacher's Signature. Teacher's Remarks.

Date 1-<J- ':Jan - 1 Page No. ~"J. .

-

JUhi . I :1. ,.-· Oo d~lthfnine . -M~ 9olCJ6/l/ly · &111Jolc. oet'<I a} d/t/'~11/. l~m~N/J 1 t, on r/. lo? <1t>rl <t~IVrm,:n, . (1/s,olu.l./<m ~U'a I

:- ~ea~e1r , Cf/CJ?) 'n:ll, ·rna/-ch 6ox, IJum.,n 6~->-n&'t', >te, waJvr 6a.M,

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\

~-~ :- ?1>, eo/u6,'II~ o~ CJny ruJn.lonre 9e1>~ !nef'ttll';U I I I uJ I M ,,, Crt.lV>e. '/) -lern/>thfl.k!'>:C. , ,'6 -~e rolu6,'l1').y . a}- d,'P~nf -lunf.)ettlhl?Ua J.l,c,,, . . ~t C/'Jn ~'l/.,'rr, a k : .. , d,.sro !uJ./on fr!' '' 1-/~al- O/J !'q/i.1/-,'(Y) •: hP~ we .· .•rkl*l'l!J t,er.,~o/c ()cl~ '10 J/1 COIi(, t'n J.Y'ltl)C>"rt ti l'olu h'on CPn <ie. elemm'·- Mr/ only 6v lllwlHll(J r:t9t,111t} 8hnebrd SoluJ,'on Ob /,'ke NaO}f.

. . '· ·• . .. ; - q. ~ =- We I><,«!./(, &r,lw,,ine Me ~olu6!1//y o0 6en~oic acid Cd -Aue o'il'f+>U>/. I -ltmPfhMI~ r' •e. ·.. 10°c ond lf/o . 0 c. ./o't- 11,/r we -trn/J<n< ./wo dl/!~nJ. • . • • • I I rolul-t'ol) al- '90 9c . (11)(/ /0 9 ( r,,t,A Je~N~e 'Y . I 'I oJ prw../r>O,"rt gei/.uwi. ~cl r ,cluh'on 06 l:t!11~0/c - CJ_cld, /,-ea/. I-he. Solu././01, . .. ll upkJ "I~- 5"0 °c , wail- /../fl Hie f o /ul-,'on ,°? C•OI d,wn kJ ,qo 0 c . j I •. . . • ' I (!Jk~~p ,;, rn,'nrl ./1,e falu/.lon hken tl,ou/r/ he af </O"l. eUM riun'1, I f}~ ~on ~<fn? _ fo,, kp_~p in ; r, - -M~/-/c. w 5'-~ &'!J.f-1, 'I I ca; DY'HnrJ Me /nrleh> , ')Q/.t,r(J.J.l.J..t Some 6en~olc.. act'tl tJJltl be Sep-I - en-okrJ t',., 1-vr iI- ~lll(I l','!-lvr pt:1.f>Vr.

1. · ~o•c I 2> t0°C. I . I , • I • r. ___J I :- volu~ reJJtlJft8 b,nHc. (' ~ '/6t()/ , om/ , .,d. ~,,/u&'i!,"&_ t1J A./o 0c. : - - n,,.;.r,uJla us,d :, N, V,: /J., V.t ·... -.,:,~· i.1, .: ? · , "'": "'150 fJoOH ,' Vi -~ ·,om/ . · aemolc (Jc,'r/ OJ 11,-. , I , ./v, ·: At..rid;tj -;-,,bv-,..eHt (vol~ -~ -olJ 1-1& ·0>-1)'· ..... .:. •N,1. V~ -;> N, ,t lo -= 1 ~/6 . N, = o -o8J U"°"' It; f • JJ, V' : I 50 . · O">'- •·· O· O~t :) · ~ •Cont,n~·/.;~ · ot, ·b;h r olc ' ac,'t af 1./o•c h 0, 0 o&~ 9 hoo.,,.,. /0 fJ1V, = NJVJ ~) N, ~to~ j_ Kt-=) N,-: O•Ot 9~m/lil:. jp 9"'r- --0 -- 0~-< ·o.0 t -;) u c·onren/..tJ.h 'o,( o! lO •c ·,.r ··o, ooj rJ'tf''l? pa 100ml ~ , (0 fM,J ?oke /om, . 06 !Jll~k ond 1-lh-o/.t. ii- OcJbt'11~J.. N loo 9~rr:lf>1d &0l11 · l.----I - N•0'1> Nr,0/-1, U1!1)8 MMo/!Jl,/-1,al,'11 Q,9 /r,c{/co.h-r '

JOml 10ml.

CS') ~f)cY't< o?IW Qolu/./on, 6v IJUM''}J Mt faltltt0.kd f'o/v/;ot, oJ ,ooc., bf./ U/')llf) ir 6qJ.I, , h&"t<. a9c,,',, ~o,ne 6en,10/c aclr/ &el- se~d'' v1>,•11-&r //. U;;,/,,& J)lllvr /J r,f)th- .

(6) 0(7,+e IO,r,I , 06 J/lkhtt/~ (JI)(/ -lihtt/-( IJ Qfjr,,'/, JJ DkJn(lm · fJ/1'0 to/ Nao4 Um"\9 /Jlluio/pMaJe,~ aa !nrt !e~ltfr.

3- :- 8clu6il,'J.v 06 ~m~ott odd Qf '(Jo 0~ ls 0 ·00.8 <. [}'t()m /loom I ,~olu6il l7to6 ~tMu i t aclcl a.I lo 0c • o,~ooi. &Tom/ 1oom 1• IS -