Plasmid DNA Extraction

Introduction

A time-saving approach from traditional column-based extraction to automatic extraction

Complete the automatic extraction within 40 minutes

Concentration and quality comparison of different plasmid DNA extraction kits (6PE, Q-brand).

(A) High copy number plasmids (pUC18) (B) low copy number plasmids (pBR322) extracted by different extraction kits using Nanodrop.

TANBead Plasmid DNA Extraction Kit is a nucleic acid purification kit based on magnetic bead technology by using with corresponding TANBeadNucleic Acid Extractor, which can automatically isolate and purify total DNA from E.coli strains. The purified plasmid can be used with any downstream application employing PCR, gel electrophoresis, sequencing, and cloning. The kit utilizes the unique non-alkaline lysis method to simplify the pre-treatment protocol of plasmid extraction. The plasmid preparation is not endotoxin-free. It is applicable for all molecular field.

Figure 1.

Agarose gel electrophoresis comparison of different plasmid DNA extraction kits (6PE, Q-brand).

(A) High copy number plasmids (pUC18) extracted by different extraction kits using agarose gel electrophoresis. After cleavage with the restriction enzyme HindIII, the band position will be located at 2,686 bp. (B) Low copy number plasmids (pBR322) extracted by different extraction kits using agarose gel electrophoresis. After cleavage with the restriction enzyme HindIII, the band position will be located at 7,211 bp.

Specification

U: Before cleavage with the restriction enzyme HindIII C: After cleavage with the restriction enzyme HindIII

Reagents Kits 40

Reagents Kits

Veterinary DNA / RNA Extraction

Introduction

High-throughput automated magnetic bead extraction technology

Highly efficient, convenient and time saving

Diverse sample range

High disease positive detection rate

Forensic DNA Extraction

TANBead Veterinary Nucleic Acid Extraction Kit is designed for the isolation of host and pathogen nucleic acid from different samples, such as stool, tissue, and serum. Our magnetic-bead based technology and corresponding extractors provide automated, high-throughput and user-friendly extraction system. The extracted nucleic acids can be applied to PCR and qPCR, etc.

Table 1.

Positive detection rates of porcine viruses in different samples by 6VT and Q- and L-brand kits.

Table 2.

Detection of new castle disease virus (NDV) gene extracted from turkey throat swab by 6VT kit

Table 3.

Positive detection rates of pathogens in aquatic products extracted by Q-brand kit and 6VT.

Figure 1.

Detection of Newcastle Disease Virus (NDV) gene extracted by 6VT and MN-brand kit.

Specification

Automated magnetic-bead based nucleic acids extraction technology

Purify limited nucleic acids from various forensic sample types

Provide choices for different sample inputs, such as 8, 48, 96 tests per rum

Reagents Kits 42

TANBead Nucleic Acid Extraction Kit (6TF) is dedicated to isolate DNA from small sample quantity or challenging sizes. Samples need to be treated by following the preparation steps, incubated in incubation buffer, and treated with Proteinase K and DTT (Dithiothreitol). The lysates need to be added into Auto Plate or Auto Tube and automatically processed using TANBead Nucleic Acid Extractor. The highly extracted and purified DNA can be directly applied for various applications, including real-time PCR, short tandem repeat (STR) analysis, mitochondrial DNA (mtDNA) analysis and other forensic tests.

Figure 1. Figure 2.

Comparison of Ct value of GAPDH obtained from different samples using different nucleic acid extraction kits.

Extraction of DNA from various samples (blood, dried blood spot, semen stain, nail, hair, chewing gum, cigarette butt, saliva swab and touched items) was performed by TANBead 6TF series and competitor nucleic acid extraction kits, Q/ M and Q/I. The Ct values of GAPDH in various samples were examined by qPCR though CFX 96 real-time PCR System. (n=4 per test)

Introduction Specification

Feature Specification

Application PCR and qPCR

Sample cigarette, hair, blood stain, dried blood spot, semen stain, chewing gun, nail

Reagent kits 6TF series

Operation time 55-75 min

DNA concentration from different samples using TANBead Forensic DNA Extraction Kit.

DNA concentrations from different kinds of forensic samples were evaluated with Quantifiler ® Trio DNA Quantification Kit. The concentration was extrapolated from the standard curve of Small Autosomal target.

Reagents