Molecular Biology Principles 2

Molecular Pathology

Molecular Biology Principles

h/p://www.interpathology.org

1

Molecular Pathology

Molecular Biology Principles

h/p://www.interpathology.org

2

Molecular Pathology

h/p://www.interpathology.org

Amongst eukaryotes, cellular DNA content does not correlate with phylogeny.!

Classifica(on of Eukaryo(c DNA

Molecular Biology Principles

3

Molecular Pathology

Molecular Biology Principles

h/p://www.interpathology.org

4

Molecular Pathology

Molecular Biology Principles

h/p://www.interpathology.org

5

Molecular Pathology

Molecular Biology Principles

h/p://www.interpathology.org

6

Molecular Pathology

Molecular Biology Principles

h/p://www.interpathology.org

7

Molecular Pathology

Molecular Biology Principles

h/p://www.interpathology.org

8

Molecular Pathology

Molecular Biology Principles

h/p://www.interpathology.org

9

Molecular Pathology

h/p://www.interpathology.org

Antibody domain structure!

H = Heavy chain!

L = Light chain!

V = variable domain!

C = constant domain!

Molecular Biology Principles

Figure 9-22!

10

Molecular Pathology

Molecular Biology Principles

h/p://www.interpathology.org

11

Molecular Pathology

Molecular Biology Principles

h/p://www.interpathology.org

12

Molecular Pathology

Molecular Biology Principles

h/p://www.interpathology.org

13

Molecular Pathology

Molecular Biology Principles

h/p://www.interpathology.org

14

Molecular Pathology

Molecular Biology Principles

h/p://www.interpathology.org

15

Molecular Pathology

h/p://www.interpathology.org

Sites A-F are separated by millions of bp of linear sequence ! but are physically close to each other in interphase nuclei!

Molecular Biology Principles

16

Molecular Pathology

Molecular Biology Principles

h/p://www.interpathology.org

17

Molecular Pathology

Molecular Biology Principles

h/p://www.interpathology.org

18

Molecular Pathology

h/p://www.interpathology.org

Consensus sequence of the minimal bacterial replication origin!

Figure 12-5!

Replication origins, regardless of organism, are (1) unique DNA segments with ! multiple short repeats, (2) recognized by multimeric origin-binding proteins, ! and (3) usually contain an A-T rich stretch!

Molecular Biology Principles

19

Molecular Pathology

Molecular Biology Principles

h/p://www.interpathology.org

20

Molecular Pathology

Molecular Biology Principles

h/p://www.interpathology.org

21

Molecular Pathology

Molecular Biology Principles

h/p://www.interpathology.org

22

Molecular Pathology

Molecular Biology Principles

h/p://www.interpathology.org

23

Molecular Pathology

Molecular Biology Principles

h/p://www.interpathology.org

24

Molecular Pathology

Molecular Biology Principles

h/p://www.interpathology.org

25

Molecular Pathology

h/p://www.interpathology.org

Linear daughter chromatids also are! separated by type II topoisomerases!

Molecular Biology Principles

26

Molecular Pathology

Molecular Biology Principles

h/p://www.interpathology.org

27

Molecular Pathology

Molecular Biology Principles

h/p://www.interpathology.org

28