Bactiquant Aquaculture Rapid testing

Aquaculture

New rapid measure for microbial water quality – total bacteria in minutes

ETV TECHNOLOGY VERIFICATION · US-EPA · 2012

A new tool for the fish farmer who wants to be in control

Rapid testing for total bacteria contribute to better fish welfare

The most advanced smolt facility in the world

A new tool for the fish farmer who wants to be in control Modern recirculating aquaculture systems are revolutionizing fish production. It also increases the demand for being in control of water quality at all stages of the production.

BactiQuant provide a US-EPA Verified rapid technology for analyzing total bacteria in minutes - BactiQuant®-water.

BactiQuant-water is primarily and foremost a microbial water quality parameter. In the fish farm, BactiQuant-water is used with a systematic approach to risc management, that originates in the food production

industry, described as Hazard Analysis and Critical Control Points (HACCP). HACCP is a systematic approach to reduce the risk of biological, chemical and physical hazards in production processes. The starting point is to select Critical Control Points (CCP’s) in the production system. Regular sampling and routine analysis are then used to establish baselines for normal operation. This allows the operator to rapidly detect critical events and focus on early detection and prevention of problems rather than the correction of problems after they have occurred.

Applications

CCP’s

Process control

Source water

Early warning

Fish tanks

Benchmarking

Hatchery

Biofilter maintenance

Before and after water treatment steps

Optimization of sanitation

Outlet

Impact evaluation of operating practices

Pump sump

MISSION CRITICAL Early warning Establish baseline for normal operation and monitor for deviations.

Verify and document Early warning allows the operator to make rapid adjustments in processes and stay in control.

Be proactive Grab samples any place in the production and get a rapid assessment of bacterial abundance.

Documented Technology: BactiQuant technology is being used worldwide and improves the quality of life for millions of people around the globe every day. The technology is well documented for use in aquaculture systems.

Picture kindly provided by Leroy, Belsvik, Norway

Rapid testing for total bacteria contribute to better fish welfare and profitability in Aquaculture This information leaflet describes a project funded by the Ministry of Environment and Food of Denmark. The Project was managed by the DTU Aqua – The National Institute of Aquatic Resources. Recycling of water and fish welfare.

The need for rapid analysis results The market for recycled aquaculture systems is growing rapidly in these years, and large land-based freshwater and saltwater production systems have been established at home and abroad. These systems reuse water and this has clear environmental benefits because water consumption is reduced and the water is cleaned. At the same time, however, the risk of microorganisms being concentrated in the recycled water is raised and in the worst case this can lead to disease outbreaks. Once bacterial growth has occurred, it should not take a week to get an analysis result. Therefore, there will be a great need for technologies that can provide a rapid assessment of the microbiological water quality.

An enzyme analysis for total bacteria A new rapid test for total bacterial analysis in water samples allows the operator to detect poor water quality in time to make rapid adjustments and maintain control of water quality. The BactiQuant method is a simple and robust rapid test, developed and patented by the Danish company, BactiQuant.

The technology was verified by the US-EPA in 2012 and has found wide spread use in water utilities and industrial applications worldwide.

The method consists of a simple measurement of a naturally occurring enzyme activity in bacteria. The BactiQuant water analysis result includes all relevant bacterial groups present in a water sample, as well as particleassociated bacteria.

Testimonial Acuinova, Land based Turbot production, Portugal

Acuinova SA is a turbot farm, potentially one of the world´s biggest land based farms. In full production we are bound to produce 3.500 tonnes of turbot, having installed capacity to quickly expand our production to 7.000 tonnes per year. We also operate at our site a flat fish hatchery producing turbot and sole juveniles in Recirculating Aquaculture Systems.

BactiQuant is a routine analysis method used at our facilities, being a precious tool to help with decision-making process.

Since we started using BactiQuant our system understanding is being much more efficient as we can identify critical points quickly and accurately, which allow us to early intervention.

Data analysis indicates changes in water quality which help us to control diseases associated with bacterial outbreaks.

We also appreciate the extraordinary support provided by BactiQuant.

Keywords for BactiQuant: rapid results with high accuracy, efficient system control, extraordinary decision-making tool and exceptional customer service.

RENATA SERRADEIRO, CEO Acuinova – actividades piscicolas, SA

The procedure itself comprises three simple steps. 1) The bacteria in a given water sample are concentrated by filtration on a sterile filter. 2) The filter is saturated with an enzyme substrate that upon degradation by a specific enzyme in bacteria is releasing a compound that emits fluorescence. 3) After a 5-10 minutes reaction the fluorescence is measured using a field portable fluorometer.

The amount of fluorescence is directly proportional to the total content of bacteria in the water sample.

New insights The new analysis method is a bit of an eye opener for relationships in aquaculture operating practices and microbial water quality. Now the operator can quantify and shed new light on processes that have not been described so far. At the DTU Aqua, the rapid method has been applied to a number of research projects in pilot scale plants, as a central measurement parameter. The analysis method was also demonstrated and implemented on a Danish model-3-fish farm and a smolt production plant in Norway. In both cases, the BactiQuant measurements provided important information about the impact and optimization of sanitizing agents, while measurements on the smolt production plant, provided new knowledge about the importance of the feed impact on water quality and the effect of biofilter maintenance.

The analysis method also provided new insights into how particles and bacteria are removed and are created in the plant.

Water quality management The starting point is to select critical control points (CCP’s) in the production systems.

Regular sampling and routine analysis is

then used to establish a baseline for normal operation in the CCP. This allows the operator to rapidly detect critical events and focus on early detection and prevention of problems, rather than the correction of problems after they have occurred. Baseline data can also be compared to the operating routines, or changed assumptions, for example, as a result of new feed, feed spill, altered operating practices for sludge drainage or recycling of biofilters.

Benefits and applications The projects have demonstrated some of the rapid method’s clear benefits and applications in the aquaculture industry. BactiQuant measurements have given a new view of bacterial dynamic causal relationships, some immediately straightforward others more complex. The method is suitable for controlled aquaculture studies and research, and is already included at the end of the project as a routine measurement method at several European knowledge institutions. Today the use of the technology, as a process monitoring tool, has been implemented at several large scale fish production facilities around the world.

Project title: Stable water quality in fish farming with new microbiological rapid test [Biostable water] Project participants: BactiQuant, Billund AquakulturService A/S and DTU Aqua.

Project period: 1/1 2014- 30/09 2016 Granted amount: 1,578,278 kr.

From GUDP Contact: Lars-Flemming Pedersen, tel: +45 35 88 32 15, email: lfp@aqua.dtu.dk

BactiQuant enjoy collaborations with Applied Aquaculture Research Institutions in Europe: • DTU Aqua the national institute of aquatic resources, Denmark • NOFIMA, Institute for applied research within the fields of fisheries, aquaculture and food research, Norway • Institut für Binnenfischerei e.V. Potsdam-Sacrow - Im Königswald, Germany • Fisheries Research Station Baden-Württemberg, Germany • Gesellschaft für Marine Aquakultur (GMA) mbH , Germany

SCIENTIFIC PUBLICATIONS: • Rojas-Tirado, P., Pedersen, P. B., & Pedersen, L. F. (2016). Bacterial activity dynamics in the water phase during start-up of recirculating aquaculture systems.

Aquacultural Engineering. http://www.sciencedirect.com/science/ article/pii/S0144860916301509 • Rojas-Tirado, P.A.,Pedersen, P.B. Vadstein, O., and Pedersen, L.F. (2016) Effects of feed loading on microbial Water quality in RAS. In international Conference on Recirculating Aquaculture, Roanoke, Aug., 2016. http://media.wix.com/ugd/9c091d_ bda94a40dd8a48f8af6a55712b95f4ab.pdf • Pedersen, L.F., and Pedersen, P.B. (2016) Microbial Water quality-tools and challenges. In International Conference on Recirculating Aquaculture, Roanoke, Wv, USA, Aug. 2016 http://media.wix.com/ugd/9c091d_ bda94a40dd8a48f8af6a55712b95f4ab.pdf • Pedersen et al. (2017) Particle surface area and bacterial activity in recirculating aquaculture systems. http://www. sciencedirect.com/science/article/pii/ S0144860917300092

• Pedersen, Per Bovbjerg; von Ahnen, Mathis; Fernandes, Paulo; Naas, Christopher; Pedersen, Lars-Flemming; Dalsgaard, Anne Johanne Tang. Room for all? – Particulate surface and bacterial activity in RAS in 3rd Nordic RAS workshop on recirculating Aquaculture Systems Molde, Norway, 30 september – 1 October 2015: Book of abstracts. ed./ Dalsgaard Anne Johanne Tang, Technical University of Denmark.

National Institute of Aquatic Resources, 2015, p. 28 DTU Aqua Report; No. 3012015).

• Christopher Naas (2016) Effects of varying biocarrier circulation intervals and methanol dosages on the denitrification performance of a SIDReactor. Fishery Science and Aquaculture.

Humboldt-Universität zu Berlin.

Lebensswissenschaftliche Fakultät.

• Christopher Naas (2014) Organic matter removal in a free Water Surface Wetland of a Trout Farm. Institut für Tierzucht und Tierhaltung Agrar- und Ernährungswissenschaftliche Fakutät der Christian-Albrechts-Universität zu Kiel.

• Rojas-Tirado,Paula Andrea (2018) Microbial water quality within Recirculating Aquaculture systems. PhD thesis, Section for Aquaculture, National Institute of Aquatic Resources, Technical Univeristy of Denmark.

One of the most advanced smolt facilities in the world Leroy Seafood Group is a world-leading seafood corporation with a history reaching back to 1899.

grow out. The smolt production chain starts with the fertilized eggs and the fish are reared to 80g within 39 weeks.

The Group’s core business is the production of salmon and trout, catches of whitefish, processing, product development, marketing, sale and distribution of seafood. The 3.800 employees deliver seafood corresponding to five million meals every year in more than 80 countries around the world.

An important focus for the Leroy smolt operation is to maintain a stable production environment. The BactiQuant-water technology is used to monitor microbial water quality at all stages of production.

The applications include, monitoring feeding impact, filter functions, verification of cleaning procedures, improving hydrodynamics in tanks and catching any irregularities in the microbial water quality.

Leroy is operating one of the world’s largest smolt facilities, located west of Trondheim. The facility has an area of 11.000 square meters, and consists of 11 separate departments, with three hatcheries and eight departments for

Julia Fossberg, Biologist, Lerøy Midt AS

- Revolutionizing microbial monitoring BactiQuant also offer the technology in an online version, which promptly quantifies total bacteria in water systems and sends out warning to the users affected when bacteria level is out of specification. The BactiQuant online system makes use of a statistical process control (SPC) algorithm. BactiQuant online can be implemented at main control points throughout the water system network

and critical points in industrial plants, and thus, sample the water quality at predetermined intervals and report to a control database.

The technology is based on our handheld technology and the unique combination of handheld and online technology will provide an unprecedented comprehensive capability to react with maximum flexibility in a pollution event.

Bacterial level (BQW-value)

Online data from an RAS farm Online data from an RAS farm.

25000

Technical issues with pumps

20000 15000 10000 5000 0 26-12-2020

Normal baseline 02-01-2021

09-01-2021

16-01-2021

23-01-2021

30-01-2021

06-02-2021

13-02-2021

Date and �me

ADVANTAGES: Eliminates labor intensive sampling and analysis - easy to install and move around Online bacterial monitoring/ minute based 24/7 results. analysis results reported within minutes of the analysis.

High accuracy. Larger sample sizes with bacteria concentrated through filtration minimizing the risk of false positives.

Early warning system. Results accessible from all platforms. Sets off an alarm if the result is deviating from normal.

The unique combination of handheld and online technology will provide an unprecedented comprehensive capability to react with maximum flexibility in a pollution event.

BactiQuant online also detects bacteria associated with particles and bacteria that are difficult to culture on plates

Dr. Neergaards vej 3 DK-2970 Hoersholm

Tel. +45 39 16 10 72 info@bactiquant.com

www.bactiquant.com