Microbiology Testing Guide - Cannabis Industry

Microbiology Testing Guide Cannabis Industry NEOGEN.com

Microbiology Testing Microbiology testing is a critical way to ensure the effectiveness of your product safety and quality programs. With a clear understanding of the microorganisms in your facility, you can better take steps to mitigate the risks they pose, better protecting your product and your brand reputation. And increasingly across regions, regulations require that all manufacturers take steps to ensure that products are free of pathogen contamination.

NEOGEN® is a leading provider of solutions that quickly and easily detect microorganisms, with over 35 years of experience in the field.

We offer numerous solutions that can be used in-house to detect the presence of, and total counts of: • Total aerobic bacteria

• Salmonella

• Coliform

• Pseudomonas

• E. coli

• Yeast and mold

• Enterobacteriacea The system and vials are validated against USP methodology, including USP 61, 62, 1223, 2021, 2022, and 2023. The software is 21 CFR Part 11-compliant with LIMS compatibility and audit trail.

Our customers are our partners, and providing a product or service is only part of the journey. We pledge to work with you to select the right solution for your needs, and to help integrate it into your workflows. Seeing you through to your goals with dedicated customer service and test training completes the picture.

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Soleris® Next Generation Soleris® Next Generation (NG) can help facilities bring microbiological testing in-house, or make their existing microbial testing simpler, faster, and automated — saving time, labor, and money. Soleris runs a comprehensive range of tests to detect microbes in a variety of testing matrices, and provides a certificate of analysis within 48 hours, with most test results achieved simply overnight. Tests are easy to run, with no need for advanced microbiological training.

The system works by reading color or fluorescence changes as target microorganisms grow in a broth medium, all within ready-to-use test vials.

The Whole System: Soleris NG Instrument • Incubates test vials while monitoring each vial • Simultaneously run up to 32 vials per instrument at 15–50°C • Up to 32 instruments linked can run 1,024 samples at once Soleris Vials • Disposable, ready-to-use vials containing growth media and indicators • Vial design prevents product interference • Each vial type targets different microbe Software • Early warning — automatically shows results as detections occur • The greater the contamination level, the faster the results • Minimize data entry errors — documents and stores data for audit trails Validated Methodology • Using USP Standards • Applicable for EP, USP, and WHO Herbal and botanical requirements

© NEOGEN Corporation, 2021. NEOGEN, ANSR, HARLEQUIN, ISO-GRID, Soleris, and µPrep are registered trademarks of NEOGEN Corporation, Lansing, MI 48912 U.S. Cannabis-Microbiology_Guide_V2

Soleris® NG makes testing for microorganisms simple.

Step One Add sample to a Soleris vial Step Two Add the vial to the machine Step Three Get results

READING ZONE

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NEOGEN® Culture Media From a library of over 315 formulations, NEOGEN® Culture Media offers many high-quality solutions for the cannabis industry, including the Harlequin® line of chromogenic (or color-based) media. Our team is always innovating this product line alongside the evolving standards of microbiological testing, keeping you up to date with the latest formulations.

ISO-GRID® ISO-GRID® is a Health Canada-approved membrane filtration system for the detection, confirmation, and enumeration of microorganisms that include, among others: Diluent

Sample

• Yeast and mold • Total plate count • Coliforms • E. coli, including E. coli O157:H7 • Salmonella

Sample and diluent

Pre-filter

• Staphylococcus • Listeria, including L. monocytogenes

Target organism

Convenience Media

ISO-GRID membrane filter in place Diluent is drawn out, leaving target organism on ISO-GRID membrane filter

Stop the waiting and start testing with NEOGEN’s convenience media products. EZ Prep and µPrep® make weighing and autoclaving a thing of the past. Watch here.

ISO-GRID membrane filter is removed from filtration unit and placed onto agar plate to incubate © NEOGEN Corporation, 2021. NEOGEN, ANSR, HARLEQUIN, ISO-GRID, Soleris, and µPrep are registered trademarks of NEOGEN Corporation, Lansing, MI 48912 U.S. Cannabis-Microbiology_Guide_V2

ANSR® Pathogen Detection ANSR®, or amplified nucleic single temperature reaction, system uses a unique DNA amplification technique to detect pathogens like Salmonella or E. coli. ANSR exponentially amplifies the DNA of any target bacteria present in an enriched food sample, bringing it to a detectable level rapidly. The system takes care of running the test at a constant temperature, as well as reading the results.

ANSR is available for: • E. coli • Salmonella • Campylobacter • E. coli O157:H7 • Listeria, including L. monocytogenes ANSR Listeria Right Now™ is the first-ever one-hour test for detecting Listeria, including L. monocytogenes, in environmental samples for environmental monitoring purposes, allowing facilities to root out harborage niches for the pathogen faster than ever before.

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A special lysis reagent is added to the enrichment sample.

Target pathogen DNA or RNA is released through the lysis step.

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The lysed sample is added to ANSR reagents. A special primer targets specific regions of the pathogen DNA and starts amplification of the DNA, creating millions of copies in minutes.

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The amplified segments of the pathogen DNA bind to special molecular beacons which then fluoresce and are detected by the ANSR reader.

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Results will be reported in 10 minutes for Salmonella, L. monocytogenes, and E. Coli 0157:H7, or 18 minutes for Listeria spp. and Campylobacter, and displayed as positive, negative, or invalid.

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LabLive LabLive is a free training and troubleshooting web service that links you directly to a NEOGEN product specialist for an individualized, interactive session. Upon scheduling a session, we’ll mail you a portable webcam that you can use to demonstrate proficiency for certification, learn new assay techniques, and troubleshoot with our experts.

Live and personal — no travel required.

Learn more online.

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