MicroSynergies Postbiotic Solutions

Postbiotic Solutions Postbio+cs, Parabio+cs, and Paraprobio+cs for situa+onal targe+ng and produc+on stability

For years, MicroSynergies has been a leader in providing a diverse por?olio of beneficial species and strains across mul+ple industries. In fact, it’s true that we have access to more microbes than anyone else on the planet.

To complement our por?olio of bioac+ve microbes, which naturally produce postbio+cs of their own, we’ve curated a line of postbio+cs to help our customers diversify their offerings and address specific issues and maintain efficacy through more harsh processing environments, such as extreme heat and cold.

Summary of Benefits Postbio(cs can provide greater stability and target metabolite survivability in more extreme product processing environments.

Postbio(cs that address specific issues can be used to target solu(ons with great potency.

We also offer live microencapsulated bacteria via our MicroCoat™ line of processing-stable microbes.

The world of postbio+cs is broken down into three main categories, and we offer solu+ons in each of these key areas: •

Postbio8cs: Harvested single metabolites from bacteria fermenta+on.

•

Paraprobio8cs: Whole-cell, inac+vated microorganisms that are treated in a specific way to target the release of an intended metabolite.

•

Parabio8cs: Inac+vated microbial cells, which can be both intact and inten+onally lysed (damaged) cells.

OUR OFFERINGS

MicroPost TM

Postbio(c Metabolites

ParaProTM

Paraprobio(cs

ParaPost TM

For more informa+on, contact MicroSynergies at bugs@microsynergies.com.

Parabio(cs

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ParaPro Postbiotics Line TM

Whole-cell, inac+vated microorganisms treated specifically to release intended metabolites.

ParaPro™ LPC Standard™ Heat-killed Lactobacillus paracasei Item Code: LACPAR-100B-PPR-hC1P Inac+vated Cells per Gram: 100 billion Postbio+cs, Parabio+cs, and Paraprobio+cs for situa+onal targe+ng and produc+on stability Func8ons • Inac+vated cells adhere to intes+nal epithelial cells, forming biofilms, and outcoming pathogenic organisms.

• Not inac+vated by an+bio+cs like live probio+cs.

• Rich in B vitamins, s+mula+ng the growth of organic acid producing genera such as Bifidobacterium, Lactobacilli, and others.

• Has a regulatory effect on non-specific immunity of intes+nal mucosa.

• Ability to absorb mycotoxins in food.

ParaPro™ Spectrum Standard™ Heat-killed Lac+c Acid Bacteria Consor+um Item Code: SPECTR-100B-PPR-hC1P Inac+vated Cells per Gram: 100 billion Func8ons • Inac+vated cells adhere to intes+nal epithelial cells, forming biofilms, and outcoming pathogenic organisms.

• Not inac+vated by an+bio+cs like live probio+cs.

• Exhibits an+-inflammatory, an+prolifera+ve, and an+oxidant proper+es.

• Exerts antagonis+c effect against pathogens.

For more informa+on, contact MicroSynergies at bugs@microsynergies.com.

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ParaPro™ LRH Weight Management™ Heat-killed Lactobacillus rhamnosus Item Code: LACRHA-0000-PPR-hC4U Inac+vated Cells per Gram: Spec Func8ons • Inac+vated cells adhere to intes+nal epithelial cells, forming biofilms, and outcoming pathogenic organisms.

• Not inac+vated by an+bio+cs like live probio+cs.

• Regulates brown fat lipid metabolism and thermogenesis.

• Down-regulates the expression of the fat synthase gene, FASN. • Inhibits lipid synthesis in the liver.

• Increases the expression of ATGL and HSL encoding genes for key catabolic enzymes of lipid metabolism in the liver.

• Promotes the produc+on of free fa_y acids.

• Provides a substrate for non-shivering thermogenesis.

• Elevates expression of the brown fat peroxisome proliferator-ac+vated receptor (PPAR) gene, PPAR-γ, and peroxisome proliferator-ac+vated receptor-γ coac+vator-1α gene, PGC-1α, increasing non-shivering in brown fat heat produc+on.

• Significantly increases the propor+on of small adipocytes (diameter ≤50µm) in epididymal white fat.

• Decreases the propor+on of large adipocytes (diameter ≥70µm).

• Effec+vely reduces the number and volume of lipid droplets in brown adipose +ssue.

• Alleviates fat accumula+on.

• Maintains normal morphology and func+on of brown adipose +ssue.

ParaPro™ LPL Anti-Heliobacter™ Heat-killed Lactobacillus plantarum Item Code: LACPLA-0000-PPR-hC4U Inac+vated Cells per Gram: Spec Func8ons • Inac+vated cells adhere to intes+nal epithelial cells, forming biofilms, and outcoming pathogenic organisms.

• Not inac+vated by an+bio+cs like live probio+cs.

• Resistant to Helicobacter pylori infec+on.

• The base strain grows in ar+ficial gastric juice with pH 2.5 and in medium with bile salt concentra+on of 0.2%, indica+ng that the strain can maintain high ac+vity in the intes+nal tract of the host.

For more informa+on, contact MicroSynergies at bugs@microsynergies.com.

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• • • • • •

The base strain has a strong ability to inhibit the growth of Helicobacter pylori and can significantly reduce the urease ac+vity of Helicobacter pylori.

Through the establishment of a Helicobacter pylori infec+on animal model, it was found that LPL An+-Heliobacter™ could significantly alleviate H. pylori infec+on in mice, and the an+-Helicobacter pylori IgG an+body level decreased.

Content of Helicobacter pylori in gastric +ssue was decreased by interven+on of H. pylori coloniza+on and prevented pathological changes of gastric +ssue.

At the same +me, LPL An+-Heliobacter™ can reduce the content of H. pylori in the mouth, adjust the balance of oral flora, and promote oral health.

The urease ac+vity of Helicobacter pylori significantly decreased ajer LPL An+Heliobacter™ treatment.

H. pylori fermenta+on broth, when treated LPL An+-Heliobacter™ for 2 hours, significantly decreased the ability of H. pylori to adsorb intes+nal epithelial cells.

ParaPost Postbiotics Line TM

Inac+vated microbial sells, both in whole and inten+onally lysed format.

ParaPost™ CS-Cell Standard™ Heat-killed Saccharomyces cerevisiae Item Code: SACCER-CELL-PAR-sB1i Inac+vated Cells per Gram: Func8ons • Inac+vated cells adhere to intes+nal epithelial cells, forming biofilms, and outcoming pathogenic organisms.

• Not inac+vated by an+bio+cs like live probio+cs.

• Offers great protec+on across a wide spectrum of Salmonella species.

• Product Cross-Makeup: o 55% Carbohydrate o 31% Crude Protein o 35% Beta Glucans o 20% MOS o 1-2% Fats • Recommended final product inclusion rate: 0.1-0.3%

For more informa+on, contact MicroSynergies at bugs@microsynergies.com.

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ParaPost™ CS-Hydro Standard™ Heat-killed Saccharomyces cerevisiae Item Code: SACCER-HYDR-PAR-sB1i Inac+vated Cells per Gram: 15,000 Func8ons • Inac+vated cells adhere to intes+nal epithelial cells, forming biofilms, and outcoming pathogenic organisms.

• Not inac+vated by an+bio+cs like live probio+cs.

• Offers great protec+on across a wide spectrum of Salmonella species and E. coli.

• Increases diges+bility.

• Source of free nucleo+des and nucleosides, glutamine, pep+des, short-chain poly pep+des, beta-glucans, and MOS. • Product Makeup: o 41% Crude Protein o 23% Beta Glucans o 15% MOS o 6% Nucleo+des o 16% Other cell wall/metabolites including: thiamine (B1), riboflavin (B2), pyridoxine (B6), cobalamin (B12), folic acid, bio+n, choline, calcium, phosphorus, potassium, magnesium, and sodium.

• Recommended final product inclusion rate: 2-5%

ParaPost™ CS-Auto Standard™ Heat-killed Saccharomyces cerevisiae Item Code: SACCER-AUTO-PAR-sB1i Inac+vated Cells per Gram: 20 billion Func8ons • Inac+vated cells adhere to intes+nal epithelial cells, forming biofilms, and outcoming pathogenic organisms.

• Not inac+vated by an+bio+cs like live probio+cs.

• Product Cross-Makeup: o 40% Metabolites including: thiamine (B1), riboflavin (B2), niacin (B3), pantothenic acid (B5), pyridoxine (B6), choline (B7), bio+n (B8), folic acid (B9), and cobalamin (B12)… as well as many amino acids. o 35% Crude Protein o 25% Beta Glucans o 15% MOS For more informa+on, contact MicroSynergies at bugs@microsynergies.com.

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MicroPost Postbiotics Line TM

Harvested single metabolites from bacteria fermenta+on.

MicroPost™ EZ-Protein Standard™ Harvested fermenta+on extract from (see tables that follow) Item Code: Varies Concentra+on per Gram: Varies Func8ons • Not inac+vated by an+bio+cs like live probio+cs.

• Enzyme breaks down proteins in the gut.

• Various MicroPost™ EZ-Protein Standard™ products for specific targe+ng located in tables that follow.

MicroPost™ EZ-Lipid Standard™ Harvested fermenta+on extract from (see tables that follow) Item Code: Varies Concentra+on per Gram: Varies Func8ons • Not inac+vated by an+bio+cs like live probio+cs.

• Enzyme breaks down fats in the gut.

• Various MicroPost™ EZ-Lipid Standard™ products for specific targe+ng located in tables that follow.

MicroPost™ EZ-Carb Standard™ Harvested fermenta+on extract from (see tables that follow) Item Code: Varies Concentra+on per Gram: Varies Func8ons • Not inac+vated by an+bio+cs like live probio+cs.

• Enzyme breaks down carbohydrates in the gut.

• Various MicroPost™ EZ-Carb Standard™ products for specific targe+ng located in tables that follow.

For more informa+on, contact MicroSynergies at bugs@microsynergies.com.

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MicroPost™ EZ-Protein™ Targeted Formats Product Name

Organism

EZ-Protein ANASP™ EZ-Protein BL-AP™

Aspergillus niger

EZ-Protein AN-FP™

Aspergillus niger

EZ-Protein AOFPA™ EZ-Protein AOFPHU™ EZ-Protein AOFPHUT™ EZ-Protein BS-NP™

Aspergillus oryzae

EZ-Protein AOPPD™

Aspergillus oryzae

EZ-Protein AMPAM™ EZ-Protein AOPXQ™

Aspergillus melleus

Bacillus licheniformis

Aspergillus oryzae Aspergillus oryzae Bacillus sub6lis

Aspergillus oryzae

Func3on A complex mixture of both exo- and endo- proteases that hydrolyzes proteins.

Cleaves pepCde bonds at random within the protein structure.

Hydrolyzes proteins at low pHs. A recommended alternaCve to animal derived Pepsin.

A complex mixture of both exo- and endo- proteases that hydrolyzes proteins.

A complex mixture of both exo- and endo- proteases that hydrolyzes proteins.

A complex mixture of both exo- and endo- proteases that hydrolyzes proteins.

Cleaves pepCde bonds at random within the protein structure.

A complex mixture of both exo- and endo- proteases that hydrolyzes proteins. Contains high exo-protease (pepCdase) acCvity.

A complex mixture of both exo- and endo- proteases that hydrolyzes proteins.

A complex mixture of both exo- and endo- proteases that hydrolyzes proteins. Contains high X-prolyl aminopepCdase acCvity.

MicroPost™ EZ-Lipid™ Targeted Formats Product Name

Organism

EZ-Lipid RO-L™

Rhizopus oryzae

EZ- Lipid AN-LAN™

Aspergillus niger

EZ- Lipid AO-LG™

Aspergillus oryzae

EZ- Lipid CC-YL™

Candida cylindracea

Func3on Cleaves triacylglycerides at the 1-, 2-, or 3-posiCon producing a mixture of di- and monoglycerides and free faVy acids.

Cleaves triacylglycerides at the 1- or 3-posiCon producing a mixture of di- and monoglycerides and free faVy acids.

Cleaves triacylglycerides at the 1- or 3-posiCon producing a mixture of di- and monoglycerides and free faVy acids.

Cleaves triacylglycerides at the 1-, 2-, or 3-posiCon producing a mixture of di- and monoglycerides and free faVy acids.

For more informa+on, contact MicroSynergies at bugs@microsynergies.com.

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MicroPost™ EZ-Carb™ Targeted Formats Product Name

Organism

Func3on

EZ-Carb AN-AG™

Aspergillus niger

EZ-Carb AN-AMY™

Aspergillus niger

EZ-Carb BA-BA™

Bacillus amyloliquefaciens

EZ-Carb TR-BG™

Trichoderma reesei

EZ-Carb AN-AGF™

Aspergillus niger

EZ-Carb TR-CLS™

Trichoderma reesei

EZ-Carb AN-CAN™

Aspergillus niger

EZ-Carb AO-FMY™

Aspergillus oryzae

EZ-Carb AO-LAC™

Aspergillus oryzae

EZ-Carb AN-HMC™

Aspergillus niger

EZ-Carb AN-INL™

Aspergillus niger

Hydrolyzes hemicellulose, a complex mixture of polysaccharides including galactomannans, glucomannans, mannans, xylans, and arabinoxylans.

Breaks down inulin to glucose and fructose.

EZ-Carb SC-INV™

Saccharomyces cerevisiae

Breaks down sucrose to glucose and fructose.

EZ-Carb AN-PCT™

Aspergillus niger

Breaks down pecCn, a polysaccharide in cell walls.

EZ-Carb TL-XYS™

Trichoderma longibrachiatum

Hydrolyzes linear beta-1,4-xylan producing xylose.

Hydrolyzes the terminal -1,6-glycosidic bonds in oligosaccharides liberaCng simple sugars.

SequenCally hydrolyzes terminal α-1,4 and α-1,6glycosidic bonds from the non-reducing ends of starch chains.

Randomly hydrolyzes glycosidic bonds within the starch chains. Typically used with Amyloglucosidase for complete starch hydrolysis.

Hydrolyzes the -1,3-glycosidic bonds found in betaglucans in cell walls.

Hydrolyzes the terminal -1,6-glycosidic bonds in oligosaccharides liberaCng simple sugars.

Hydrolyzes both internal and external beta-glycosidic bonds in cellulose. Contains all three cellulolyCc acCviCes: endo-, exo-cellulases and glucosidase.

Hydrolyzes both internal and external beta-glycosidic bonds in cellulose. Contains all three cellulolyCc acCviCes: endo-, exo-cellulases and -glucosidase.

Randomly hydrolyzes glycosidic bonds within the starch chains to produce soluble dextrins, maltose and glucose.

Breaks down lactose to glucose and galactose.

For more informa+on, contact MicroSynergies at bugs@microsynergies.com.

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