Database & Online Map of Sea Turtle Rescue & First Aid Centres in the Mediterranean

EDITORS Bojan Lazar, Matic Jančič

BOOK OF ABSTRACTS

6TH MEDITERRANEAN CONFERENCE ON MARINE TURTLES, POREČ, CROATIA 2018

Book BookofofAbstracts Abstracts

Editors: Editors:

Organizer Organizerand andhosting hostinginstitution institution

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Bojan BojanLazar Lazar

Department DepartmentofofBiodiversity Biodiversity

Blue BlueWorld WorldInstitute InstituteofofMarine MarineResearch Researchand andConservation Conservation

Valamar ValamarDiamant DiamantConference ConferenceCentre Centre

Matic MaticJančič Jančič

Faculty FacultyofofMathematics, Mathematics,Natural NaturalSciences Sciences Veli VeliLošinj, Lošinj,Croatia, Croatia,EU EU

Poreč, Poreč,Croatia Croatia Design: Design: Ideodizajn Ideodizajn/ /ideo.hr ideo.hr

and andInformation InformationTechnologies Technologies

www.blue-world.org www.blue-world.org

University UniversityofofPrimorska Primorska Koper, Koper,Slovenia, Slovenia,EU EU

Supporting SupportingOrganizations Organizations

www.famnit.upr.si www.famnit.upr.si

UN UNEnvironmental EnvironmentalProgram Program––Mediterranean MediterraneanAction ActionPlan Plan Regional RegionalActivity ActivityCenter Centerfor forSpecially SpeciallyProtected ProtectedAreas Areas Council CouncilofofEurope Europe––Bern BernConvention Convention

ISBN: ISBN:978-953-6645-83-1 978-953-6645-83-1 CIP CIPcatalogue cataloguerecord recordisisavailable availableatatthe theNational Nationaland andUniversity UniversityLibrary LibraryininZagreb Zagrebunder underthe thenumber number001009011 001009011 Publisher: Publisher:Croatian CroatianNatural NaturalHistory HistoryMuseum, Museum,Zagreb, Zagreb,Croatia Croatia(2018) (2018) For Forthe thePublisher: Publisher:Tatjana TatjanaVlahović Vlahović

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©University ©UniversityofofPrimorska Primorska Titov Titovtrg trg4,4,SI-6000 SI-6000Koper, Koper,Slovenia, Slovenia,www.upr.si www.upr.si

President President

Steering SteeringCommittee Committee

Bojan BojanLazar Lazar(University (UniversityofofPrimorska) Primorska)

Bojan BojanLazar Lazar(University (UniversityofofPrimorska) Primorska)

No Nouse useofofthis thispublication publicationininwhole wholeororininpart partisisallowed allowedfor forsale saleororfor forany anyother othercommercial commercialpurpose purposewithout withoutwritten written

Conference Conferencesecretary secretary

Draško DraškoHolcer Holcer(Blue (BlueWorld WorldInstitute) Institute)

Matic MaticJančič Jančič(University (UniversityofofPrimorska Primorska

Lobna LobnaBen BenNakhla Nakhla(UNEP (UNEPMAP-RAC/SPA) MAP-RAC/SPA)

&&Blue BlueWorld WorldInstitute) Institute)

Veronique VeroniquededeCussac Cussac(Bern (BernConvention) Convention)

Organizing OrganizingCommittee Committee

Scientific ScientificCommittee Committee

Bojan BojanLazar Lazar(University (UniversityofofPrimorska) Primorska)

Paolo PaoloCasale, Casale,co-chair co-chair(University (UniversityofofPisa, Pisa,Italy) Italy)

permission permissionbybythe theUniversity UniversityofofPrimorska.

Primorska.This Thispublication publicationmay maybebeused usedfor foreducational educationalpurpose purposewithout withoutspecial specialpermispermission sionfrom fromthe thecopyright copyrightholder, holder,provided providedacknowledgement acknowledgementofofthe thesource sourceisismade. made.

The TheMediterranean MediterraneanConferences ConferencesononMarine MarineTurtles Turtlesare arejoint jointinitiatives initiativesbybythe theSecretariats Secretariatsofofthe thefollowing followingConvenConventions: tions: • •Convention Conventionfor forthe theProtection Protectionofofthe theMediterranean MediterraneanSea Seaagainst againstPollution Pollution(Barcelona (BarcelonaConvention) Convention) • •Convention Conventionononthe theConservation ConservationofofEuropean EuropeanWildlife Wildlifeand andNatural NaturalHabitats Habitats(Bern (BernConvention) Convention) • •Convention Conventionononthe theConservation ConservationofofMigratory MigratorySpecies SpeciesofofWild WildAnimals Animals(Bonn (BonnConvention) Convention)

Draško DraškoHolcer Holcer(Blue (BlueWorld WorldInstitute) Institute)

Bojan BojanLazar, Lazar,co-chair co-chair(University (UniversityofofPrimorska, Primorska,Slovenia) Slovenia)

Disclaimer: Disclaimer:

Matic MaticJančič Jančič(University (UniversityofofPrimorska) Primorska)

Mustapha MustaphaAksissou Aksissou(Abdelmalek (AbdelmalekEssaâdi EssaâdiUniversity, University,Morocco) Morocco)

The Thedesignations designationsemployed employedand andthe thepresentation presentationofofthe thematerial materialininthis thisdocument documentdodonot notimply implythe theexpression expressionofofany anyopinion opinion

Tilen TilenGenov Genov(University (UniversityofofPrimorska) Primorska)

Luis LuisCardona Cardona(University (UniversityofofBarcelona, Barcelona,Spain) Spain)

whatsoever whatsoeverononthe thepart partofofthe theorganizations organizationsinvolved involvedininthe theorganization organizationofofthe theConference Conferenceconcerning concerningthe thelegal legalstatus statusofof

Peter PeterGlasnović Glasnović(University (UniversityofofPrimorska) Primorska)

Sandra SandraHochscheid Hochscheid(Zoological (ZoologicalStation StationAnton AntonDohrn, Dohrn,Italy) Italy)

any anyState, State,Territory, Territory,city cityororarea, area,ororofofitsitsauthorities, authorities,ororconcerning concerningthe thedelimitation delimitationofoftheir theirfrontiers frontiersororboundaries.The boundaries.Theviews views

Aleš AlešOven Oven(University (UniversityofofPrimorska) Primorska)

Carlos CarlosCarreras Carreras(University (UniversityofofBarcelona, Barcelona,Spain) Spain)

expressed expressedininthis thisvolume volumeare arethose thoseofofthe theauthors authorsofofeach eachpaper paperand anddodonot notnecessarily necessarilyrepresent representthe theviews viewsofofthe theabove above

Mitja MitjaTretjak Tretjak(University (UniversityofofPrimorska) Primorska)

Imed ImedJribi Jribi(University (UniversityofofSfax, Sfax,Tunisia) Tunisia)

mentioned mentionedorganizations. organizations.

Živa ŽivaFišer FišerPečnikar Pečnikar(University (UniversityofofPrimorska) Primorska)

Yakup YakupKaska Kaska(Pamukkale (PamukkaleUniversity, University,Turkey) Turkey)

Manica ManicaBalant Balant(University (UniversityofofPrimorska) Primorska)

ALan ALanF.F.Rees Rees(University (UniversityofofExeter, Exeter,U.K.) U.K.)

This Thispublication publicationshould shouldbebecited citedasasfollows: follows:

Martina MartinaLužnik Lužnik(University (UniversityofofPrimorska) Primorska)

Oguz OguzTurkozan Turkozan(Adnan (AdnanMenderes MenderesUniversity, University,Turkey Turkey

Lazar LazarB.B.and andJančič JančičM.M.(editors) (editors)2018.

2018.Book BookofofAbstracts, Abstracts,6th 6thMediterranean MediterraneanConference ConferenceofofMarine MarineTurtles, Turtles,Poreč Poreč- Croatia.

- Croatia.

Mateja MatejaZekan Zekan(Blue (BlueWorld WorldInstitute) Institute)

Travel Travelgrant grantchair chair

168 168pp. pp.

Jure JureMiočić MiočićStošić Stošić(Blue (BlueWorld WorldInstitute) Institute)

Aliki AlikiPanagopoulou Panagopoulou(ARCHELON) (ARCHELON)

2

3

BOOK OF ABSTRACTS

6TH MEDITERRANEAN CONFERENCE ON MARINE TURTLES, POREČ, CROATIA 2018

POSTER PRESENTATIONS SESSION 5: HEALTH AND REHABILITATION

POSTER PRESENTATIONS SESSION 5: HEALTH AND REHABILITATION

DATABASE & ONLINE MAP OF SEA TURTLE RESCUE & FIRST AID CENTRES IN THE MEDITERRANEAN

MULTIDRUG RESISTANCE IN CLINICAL ISOLATES FROM LOGGERHEAD SEA TURTLES (Caretta caretta) LIVING IN THE MEDITERRANEAN SEA

Boura L.1, Touloupaki E.1, Rae V.1, Ullmann J.2

Cirilli M.1, Trotta A.1, Sposato A.1, Marinaro M.2, Valastro C.1, Franchini D.1, Corrente M.1

1 2

MEDASSET-Mediterranean Association to Save the Sea Turtles, 1c Licavitou St., 106 72 Athens, Greece Department of Arctic and Marine Biology, Faculty of Biosciences, Fisheries and Economics, UiT The Arctic University of Norway, Framstredet 41–43, 9037 Tromsø, Norway

The “Database & Online Map of Sea Turtle Rescue & First Aid Centres in the Mediterranean” (www.medasset.org/our-projects/sea-turtle-rescue-map) is a project by MEDASSET, aiming to bridge information, communication, and collaboration gaps in sea turtle conservation. Launched in 2016, this open-access tool provides both professionals and citizens with comprehensive information about all known sea turtle rescue facilities in the Mediterranean. We aim at increasing collaborative networking among specialists and raising public awareness of the rescue centre network. Providing easy information access is one crucial step toward increased public involvement (volunteering, donations, etc.). MEDASSET’s up-to-date, visual tool is also useful for identifying gaps in the distribution of rescue and rehabilitation facilities on the Mediterranean scale and can potentially assist in conservation planning and policy-making. The project is an excellent “science to society” example, answering Ullmann & Stachowitsch’s (2015, Nature Conservation 10: 45–69) call for action and bringing their findings to both the public’s and the decision makers’ attention. The above study provided the conceptual framework and baseline data for

138

MEDASSET’s database. Facility data have since been updated and completed through an online survey targeting rescue centres and national authorities (the latter was facilitated by RAC/SPA). The map currently comprises 59 facilities in 17 countries across the Mediterranean. The alpha versions of the database and map were purposefully developed using freely available software to ensure the tool’s sustainability in an NGO context. Organisations are free to embed the map into their websites; thus, multiplying the initiative’s impact. Through the current presentation, we seek to share our lessons learnt and advance our ideas regarding further development of the map and database (interactive and/or multilingual user surface, forum, integration into other GIS databases). Striving to maximise our tool’s benefits to the conservation effort, we seek feedback from rescue centres, scientists, conservationists, and policy makers.

Acknowledgement: MEDASSET warmly thanks the volunteers who helped create the database and map: J.Judge, E.Sun, H.Tzortzakis, and E.Bountouraki.

1 2

Department of Veterinary Medicine, University of Bari, s.p. Casamassima km 3, 70010, Valenzano, Italy.

Department of Infectious, Parasitic and Immune-Mediated Diseases, Istituto Superiore di Sanità, Viale Regina Elena 299, 00161, Rome, Italy

Marine animal species may act as reservoirs of antimicrobial resistant (AMR) microorganisms. Extended-spectrum-β-lactamases (ESBLs) producers are an important category of AMR bacteria. ESBLs are enzymes that hydrolyze all beta-lactams drugs, resulting in limitation of therapeutic options. Between 2016 and 2018, 52 loggerhead sea turtles (Caretta caretta), hospitalized at the Sea Turtle Clinic (Department of Veterinary Medicine, University of Bari, Italy), were sampled for bacteriological investigation. Forty Gram-negative bacteria were isolated from cutaneous lesions (55%), bronchoalveolar lavages (32.5%) and internal lesions (12.5%). Strains were biochemically identified as Aeromonas spp. (22.5%), Vibrio spp. (20%), Citrobacter spp. (20%), Pseudomonas spp. (15%), Acinetobacter spp. (5%), Morganella spp.

(5%), Proteus spp. (5%), Klebsiella spp. (2.5%), Enterobacter spp. (2.5%), Alcaligenes spp. (2.5%). Resistance against 13 antibiotics was determined by disk diffusion methods according to EUCAST guidelines3.

Seventy-four isolates were multidrug resistant. The

isolates exhibited highest resistance to β-lactam antibiotics (87.9%), followed by quinolones, such as ciprofloxacin (67%), tetracycline (58%) Interestingly, all the isolates were resistant to Imipenem (100%), a drug for human use. Nine strains (22.5%) were resistant to all antibiotics tested. ESBLs genes of most common families (CTX, AmpC, TEM, and SHV) were screened by PCR. The most frequently detected genes were blaAmp-C (45%), blaCTX-M (17.5%) and blaTEM (12.5%), followed by blaSHV (2.5%). Five isolates (5%) simultaneously harboured 3 genes and 6 isolates (15%) 2 genes. Antimicrobial susceptibility patterns of bacterial isolates are an important information for appropriate treatment of sea turtles in rehabilitation centres. In addition, they could help tracing the origin and spread of multidrug resistant bacteria in turtles, which have a significant ecological impact on the marine environment.

Presenting Author: Margie Cirilli (margie.cirilli@uniba.it)

Presenting Author: Eleana Touloupaki (eleanatouloupaki@medasset.org)

139

DATABASE & MAP OF SEA TURTLE RESCUE CENTRES IN THE MEDITERRANEAN Liza

ORIGINS

1 Boura ,

Eleana

1 Touloupaki ,

Vicky

1 Rae ,

Judith

2 Ullmann

HOW IT WORKS

Scientific call A,B,C for: • Common database • Networking • Public involvement MEDASSET Project brings “Science to society”

DATA

• Open access, cloud database & map • Freeware (Google Sheets, Google Maps) • Open use: all can embed map in websites DATA SOURCES Conceptual framework 1 & baseline data A provided by review

2

• Maintenance by volunteers • Low cost

Baseline data update: 2016 online survey of facilities & authorities (assisted by RAC/SPA)

3

Maintenance: continually (request by facilities) periodically (survey every 2-3 yrs)

Sea Turtle Rescue Map: www.medasset.org/our-projects/sea-turtle-rescue-map

 Contact details  Year of establishment  Volunteer positions  Sea turtle statistics ..... And more!

LIMITATIONS Participation

• High among facilities • Low among national authorities (6 of 17)

BENEFITS ↑ Awareness ↑ Stranding reports ↑ Visits ↑ Volunteers ↑ Donations

STATUS  Launched Sept. 2016  61 facilities  in 17 countries  >12.000 map views! (as of Oct. 2018)

Sustainable in NGO context!

OPEN ACCESS  USER FRIENDLY  UP-TO-DATE VISUAL TOOL

Key:

Rescue Centre

WHAT’S NEXT? Facilities

• Are you on the map?

• How can we improve?

Data Quality

• Self-declared information • No screening or quality assessment of facilities by MEDASSET • No analytical statistics of map use

First Aid Station

Informal/Temporary Facility

Funders – Partners Beta Version:

• Multilingual & online forms for data update by facilities • Stylised Google Map • Integration with GIS databases & mobile apps

 Gap ID in facility distribution  Planning & policy-making

 Accessibility  Integration  Interactivity  Multiplication of Use & Impact

Gamma Version:

• Interactive multilingual microsite

• Direct editing by facilities • Forum for networking and exchange • Sophisticated statistics of map use

ACKNOWLEDGEMENTS MEDASSET warmly thanks the volunteers who helped create database & map: J. Judge, E. Sun, H. Tzortzakis, E. Bountouraki

1 MEDASSET

Mediterranean Association to Save the Sea Turtles 1c Licavitou St., 106 72 Athens, Greece Corresponding author: Liza Boura, lizaboura@medasset.org Tel: + 30 210 3613572

LITERATURE CITED

2 UiT

The Arctic University of Norway Tromsø, Norway Department of Arctic and Marine Biology judith.ullmann@uit.no

A) Ullmann J, Stachowitsch M, 2015. A critical review of the Mediterranean sea turtle rescue network: a web looking for a weaver. Nature Conservation, 10: 45–69. | B) Panagopoulou A, Rees AF, 2009. Networking among Rescue Centres in the Mediterranean. In: Demetropoulos P, Turkozan O (Eds) Proceedings of the Second Mediterranean Conference on Marine Turtles, Kemer, Antalya (Turkey), May 2005. Barcelona Convention, Bern Convention, Bonn Convention (CMS), pp 144–147. | C) Kasparek M, 2003. Proposals for Setting-Up a Clearing-House Mechanism to Monitor Marine Turtle Populations in the Mediterranean. In: Margaritoulis D, Demetropoulos A (Eds) Proceedings of the First Mediterranean Conference on Marine Turtles, Rome (Italy), October 2001. Barcelona Convention, Bern Convention, Bonn Convention (CMS), Nicosia, Cyprus, pp 151–155.