A bioassay method validation framework for laboratory and semi-field tests used to evaluate vector control tools

Introduction

• Vector control interventions play a fundamental role in the control and elimination of vector-borne diseases 1

• The evaluation of vector control products relies on bioassays and semi-field tests that use live insects, to assess the product’s effectiveness 2

• Bioassay method development requires a rigorous validation process to ensure that relevant methods are used to capture appropriate entomological endpoints 3

• Method validation: a process used to demonstrate that a procedure is suitable for its intended purpose and produce reliable results 4

• Currently, there no standardised guidelines for validating novel vector control methods

Results

Methodology

Conclusion

The proposed framework ensures that:

• A method is properly validated

• Methods are sufficiently sensitive to capture different modes of actions and can be used to assess the impact of new tools on various vector populations

• An appropriate experimental design and data analyses that account for both the variability of the method and the product being tested are employed

✓ To generate reliable estimates of product efficacy assessment

• Therefore, at-risk communities have timely access to safe and reliable vector control products

Acknowledgments

Special thanks to Professor Sarah Moore for providing an invaluable critical review of the framework and feedback

References

1. WHO. Global report on insecticide resistance in malaria vectors: 2010-2016. Geneva; 2018

2. WHO, WHO Pesticide Evaluation Scheme. Guidelines for laboratory and field testing of long-lasting insecticidal nets. 2013

3. WHO. Manual on development and use of FAO and WHO specifications for pesticides.

2016

4. ISO. ISO 9000:2015 - Quality management systems Fundamentals and vocabulary.

2015

5. Garcia PL, Buffoni E, Gomes FP, Quero JLV, Garcia PL, Buffoni E, et al. Analytical Method Validation. Wide Spectra of Quality Control. IntechOpen; 2011

6. Lubrizol Life Science. Analytical Method Development and Validation. 2019