Appl i cat i onsofCel lCul t ur e
One oft he mostessent i alt ool si n cel l ul arand mol ecul arbi ol ogyi scel lcul t ur e,whi ch of f er s super i ormodelsyst emsf orst udyi ngt het ypi calphysi ol ogyandbi ochemi st r yofcel l s( suchas met abol i cst udi es,agei ng) ,t heef f ect sofdr ugsandt oxi csubst ancesont hecel l s,andmut agenesi sandcar ci nogenesi s.Addi t i onal l y ,i ti sut i l i sedf orbi ol ogi calcompoundmanuf act ur eonabi g scal easwel lasdr ugr esear chandscr eeni ng( e. g. ,vacci nes,t her apeut i cpr ot ei ns) .Themai n benef i tofempl oyi ngcl onalcel l si ncel lcul t ur ef oranyoft heseappl i cat i onsi st hest abi l i t yand r epr oduci bi l i t yoft her esul t st hatmayobt ai n.
VACCI NE PRODUCTI ON: Thedevel opmentandmanuf act ur i ngofvacci nesar eone oft he mostcr uci alappl i cat i onsofcel lcul t ur e.The pol i o vacci newasevent ual l ydevel opedbecauseoft heabi l i t yt o gr ow vastquant i t i esofvi r usi ncel lcul t ur e,andcel l sar e bei ngempl oyedext ensi vel yt odayt omakevacci nat i onsf or manyot herdi seases.
CANCER RESEARCH: Radi at i on,chemi cal s,and vi r usesar ej usta f ew mechani sms t hatcan t ur n nor malcel l si nt o cancer ous ones. Then,t hesecel l scanbeut i l i sedt oexami necancermor e t hor oughl yandt r ypr ospect i vef r esht her api es.
MOLECULAR AND CELLULAR BI OLOGY: One oft he mostcr i t i calt ool si n cel l ul arand mol ecul ar bi ol ogy i s cel lcul t ur e,whi ch of f er s appr opr i at e model syst emsf orst udyi ngt het ypi calphysi ol ogyandbi ochemi st r yofcel l s( suchasmet abol i cst udi es,agei ng) ,t heef f ect s ofvar i oust oxi csubst ancesont hecel l s,andmut agenesi s and car ci nogenesi s.The uni f or mi t yand r epr oduci bi l i t yof t hedat aacqui r edf r om abat chofcl onalcel l si st hepr i mar y benef i t of cel l cul t ur ef or any of t hese appl i cat i ons.
GENE THERAPY: Thecel lcul t ur et echni quei susedt osubst i t ut enonf unct i onal cel l s wi t h cel l s t hat have f unct i oni ng genes.
I MMUNOLOGI CALRESEARCH: Byempl oyi ngcel lcul t ur et echni ques,sci ent i st scanl ear n how di f f er enti mmunecel l s,cyt oki nes,l ymphoi dcel l s,and t he i nt er act i ons bet ween pat hogens and hostcel l s al l f unct i on.
VI RUS CULTI VATI ON: Si nce cel l cul t ur e i s mor e pr act i cal , af f or dabl e, and manageabl et han ot herani malmet hods,i ti sf r equent l y empl oyed t or epl i cat e vi r uses.I ti s si mpl et oi dent i f y speci f i c cel l s on whi ch t he vi r us mul t i pl i es and see cyt opat hi cconsequences.
DRUG DI SCOVERY: Themostdependabl ei nvi t r oassaysar et hoset hati nvol ve cel l cul t ur es, whi ch ar e car r i ed out dur i ng t he dr ug devel opmentpr ocess.Dr ug di scover y has l ong i ncl uded t wodi mensi onal ( 2D) cel l cul t ur es. These days, t hr eedi mensi onal( 3D)cel lcul t ur es and cocul t ur es ar e r epl aci ngt hem i nt er msofi mpor t ance.