In vitro models are crucial tools in cancer research because they assist researchers to identify carcinogens, developing cancer therapies, drug discovery, and study about the molecular pathways of tumour growth and spread. Cancer cells are an essential part of any in vitro tumour model. Cancer cell lines are simple to culture, allow for quick comparisons of experiments, and are commonly employed to research tumour cell biology molecular pathways. Tumour cell biology, 3D cell culture, tissue engineering, biomaterials, microfabrication, and microfluidics advancements have facilitated the dynamic development of in vitro tumour models. Our Esteemed Clients Applications of Transwell-Based Assays Studying the influence of chemoattractants on migration and invasion
1. Migration assays: The migration assay includes planting cancer cells on top of a porous membrane and counting how many cells can migrate throughout the membrane toward a chemoattractant. T this is a simple assay, the rate of migration via pores towards serum delivers a high throughput in vitro tumour model. 2. Invasion assays: Invasion assays bring a new layer of complication to this paradigm by adding an Extracellular Matrix (ECM) layer on the porous membrane. Despite migration and invasion assays targeting relatively comparable cell attributes, it is important to note that some drugs and gene alterations have a greater effect on invasion reduction than migration reduction. 3. Transendothelial migration assays:
various cell types
Placing a confluent layer of endothelial cells onto a porous layer is used in transendothelial migration assays. The cell to cell connections between endothelial cells and the ECM that they form add intricacy to this concept. These transendothelial assays are most typically employed to research brain capillary endothelium, although other
Testing the influence of antibodies o invasion and migration of the cells
endothelial cell types, such as HUVECs, can be studied as well.
Studying the influence of other cell types such as fibroblast, macrophages on the invasion of cancer cells Isolation of invasive/non-invasive cell types for molecular analysis Studying relative rates of invasion and migration of
Transwell-Based Models The migration of cells from one region to another is fundamental to the metastatic process. Transwell assays are commonly used to evaluate cancer cell migration and invasion. The three most prevalent transwellbased assays are: