experiemnt booklet

Mycelium Experiment -

Thai Fungus Library

A fungus, fungi or funguses is any member of a large group of eukaryotic organisms. A difficult species to fully identify, with over 80,000 named species worldwide and thought to be over a million yet to be identified. Most can only be identified by microscopic study of spores and flagella.These are the common species of mushroom in Thailand.

Name ; Shitake Mushroom Thai Name ; ࡳࡢࡺࡋࡢࡤࡘ Species ; Lentinula edodes Growth Environmemt ; Winter/Rainy 28- 30c

Name ; Spotted Mushroom Thai Name ; ࡳࡢࡺࡋ࠸࡚ࡧࡋࡻࡻࡩ࠾ ࡳࡢࡺࡋࡑࡋ Species ; Lentinus polychrous Growth Environmemt ; All Year 30-3c2

Name ; Olyster mushroom Thai Name ; ࡳࡢࡺࡋࡐࡩ࠾࡚ࡘ Species ; Pleurotus ostreatus Growth Environmemt ; All Year 15 - 32 C

Name ; Jew's Ear Mushroom Thai Name ; ࡳࡢࡺࡋࡢࡰࡢࡐࡰ Species ; Auricularia auricula-judae Growth Environmemt ; All Year/ High Yield

Name ; Matsutake Mushroom Thai Name ; ࡳࡢࡺࡋ࡙ࡩࡐࡩ࠾࡫ Species ; Cyclocybe aegerita Growth Environmemt ; All Year

Name ; Milky Mushroom Thai Name ; ࡳࡢࡺࡋࡌ࡬ࡐࡴ࡚ࡋ Species ; Calocybe indica Growth Environmemt ; All Year 30-38c

Name ; Log White Mushroom Thai Name ; ࡳࡢࡺࡋ࠹ࡤࡐ࠹ࡩ࡞ ࡳࡢࡺࡋࡘࡨࡐ Species ; Lentinus squarrosulas Mont Growth Environmemt ; All Year 31c

Name ; Indian Mushroom Thai Name ; ࡳࡢࡺࡋࡐࡩ࠾ࡖࡼࡩ Species ; Pleurotus pulmonarius Growth Environmemt ; Winter/Low temperature

Name ; Enoki Mushroom Thai Name ; ࡳࡢࡺࡋࡳ࠹ࡺࡘࡎࡤ࠾ Species; Flammulina velutipes Growth Environmemt ; Winter/Rainy

Name ; Lingzhi, Reishi Mushroom Thai Name ; ࡳࡢࡺࡋࡢ࡜࡫ࡐ࠿࡮ࡤ Species ; Ganoderma lucidum Growth Environmemt ; All Year / High Yield

Name ; Straw Mushroom Thai Name ; ࡳࡢࡺࡋࡖࡩ࠾ Species ; Volvariella volvacea Growth Environmemt ; Winter/Rainy

Name ; Splitgill Mushroom Thai Name ; ࡳࡢࡺࡋࡴ࠻࡚࠾ Species ; Schizophyllum commune Growth Environmemt ; All Year 30-35 C

Name ; Straw Thai Name ; ࡖࡩ࠾ Source ; All regions of Thailand, especially in the North Eastern.

Name ; Coco Coir Thai Name ; ࠹࡯࡙ࡘࡧࡕ࡚ࡼࡩ࡞ Source ; All regions of Thailand, especially in the Southern.

Name ; Cotton fiber Thai Name ; ࡐ࡯ࡻࡐ Source ; All regions of Thailand, especially in the Northern.

Name ; Green Beans Thai Name ; ࡍࡨࡻ࡞ࡳ࠹࡬࡙࡞ Source ; All regions of Thailand, especially in the Northern.

Name ; Coffee Grounds Thai Name ; ࠸ࡩ࠸࠸ࡩࡴࡖ Source ; All regions of Thailand, especially in the Central region

Thai Substrate Library

Substrate mycelium is known as primary mycelium or vegetative mycelium and grows in both solid grown and submerged cultures. The substrate mycelium inevitably develops from the germinating spores but never produces spores itself. Many differentiate to form aerial hyphae on solid surfaces. In Thailand, thereare many substrate that commonly used are here, plus the other to explor.

Name ; Rice Husk Thai Name ; ࡴ࠸࡜ࡑ Source ; All regions of Thailand, especially in the Central region

Name ; Corn Core Thai Name ; ࡴ࠸ࡐ࠹ࡼࡩ࡞ࡵࡕࡋ Source ; All regions of Thailand, especially in the North Eastern.

Name ; Organic Tea Leaves Thai Name ; ࡶࡑࡁࡩ Source; All regions of Thailand, especially in the Northern.

Name ; Corn stalks Thai Name ; ࡌࡼࡐ࠹ࡼࡩ࠾ࡵࡕࡋ Source ; All regions of Thailand, especially in the North Eastern.

Name ; Wood Ship Thai Name ; ࡁ࡫ࡼࡐࡷࡘࡼࡡࡨࡑ Source ; All regions of Thailand, especially in the Southern part

(;3(5,0(17,1* 352&(66

Base Plate Growth

Sterilization

Cooling

Growth in Bag

Module are on the point of independence of form and fction There are vearius types of modu, each module will achieve difrent varying goals including flexibity

The grains are, at this point, still full of countless micro-organisms that would all form threats to the vulnerable mycelium when it is trying to colonize the grain. To eliminate all these competitors, we sterilize the bags of grain in a pressure cooker for 3 hours.

After sterilization, the bags are taken out of the pressure cooker and left to cool down overnight before the spawn (mycelium starter) is added. This is important because if the grainsare still hot the mycelium would be killed immediately.

Waiting for the m and processes s monitor the grow mycelium should the grains. When the bag are comp material is ready into a different sh

* Sanitize your hands and equipment on every steps to avoid contamination * The substrate should be sterlize and mix with mycelium within 7 days

mycelium to grow so far went right, wth. If all the d start colonising n the contents of pletely white, the y to be transferred hape.

Transfer to Shape

Drying

Done,

When the bags are completely colonised, the mycelium is broken up again to be transferred to the desired shape. Once transferred, the mycelium needs time for a second growth. The little strands of mycelium will grow again, glueing the substrate together, outer skin making a solid form and a white

When the growing is complete, in order to turn the living we have to heat the mycelium which kills it, stopping any organism into a biomaterial further growth. We do this by heating a de-hydrating chamber and leave the object for a few hours, drying out the material and killing the organism with the heat.

Module are on the point of independence of form and fction There are vearius types of modu, each module will achieve difrent varying goals including flexibity

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Phase 1 Cell

GL.01

LP.01

FV.01

TC.01

PO.01

SM.01

GL + TC

Pattern.02

Pattern.03

Pattern.04

Pattern.05

Pattern.06

GL.01

LP.01

FV.01

TC.01

PO.01

SM.01

GL.02

LP.02

FV.02

TC.02

PO.02

SM.02

GL.03

LP.03

FV.03

TC.03

PO.03

SM.03

GL.03

LP.03

FV.03

TC.03

PO.03

SM.03

Phase 0 Unit No nutrients just leave it grow

Sugar(glucose)+ Gypsum+ Pumice(pH)+ Magnesium

Nutrients only Magnesium+Sugar

* Sanitize your hands and equipment on every steps to avoid contamination * The substrate should be sterlize and mix with mycelium within 7 days

Phase 2 Module - (How well it take on the shape of the molded)

GL.01

FV.01

LP.01

Phase 3 Systems (Form and Function)

GL.01

GL.01

GL.01

LP.01

LP.01

LP.01

**Selected Mushrooms GL - Ganoderma lucidum

FV - Flammulina velutipes

PO - Pleurotus ostreatus

LP - Lentinus polychrous

TC - Tricholoma crassum

SM - Schizophyllum commune

Experiment 1 -

Cell

Cover

Mycelium

Agar

Container

2

1

1. Alcohol 2. Tissue 3. Microwave 4. Gloves 5. Tweezer 6. Agar 7. Tape 8. Pen 9. Container 10. Foil 11. Spoon

4

S a

7

5

9

W

8

11

3 10

6

M o

Sanitize hands and utensils

Put the Agar in micowave

Microwave for a minute

Wait for it to set

Pour agar into Petridish

Quickly add mycelium into the dish (operate this step inside the box)

Mark the code of each container

Observe and document

*** The Agar should be use within 7 days to avoid contamination

1. Case 2. Cover 3. PVC Tube 4. Gloves 5. Silicone Glue 1

N ATIO N I AM M ONT YCELIU C M M O S FR IXINNG M E T M I ING TEP OF T C S TE PRO NG THE I DUR

5

3

60

cm

* The container is fully covered to avoid contamination during operation * Everything Inside should be clean before closing the lid * None of the items contacting air from outside

4

2

m

40c

Strain

Day 1

Day 2

Day 3

Day 4

Day 5

Day 6

Day 7

Pattern.01

Pattern.02

Pattern.03

Pattern.04

Pattern.05

Pattern.06

Mix Strain

Day 1

Day 2

Day 3

Day 4

Day 5

Day 6

Day 7

Pattern.01

Pattern.02

Pattern.03

Pattern.04

Pattern.05

Lentinus

The container is fully covered to avoid contamination during

Reishi

The container is fully covered to avoid contamination during

Cell ; Conclusion Eventhough the experiment was failed to observe the pattern due to the contamination, and amount of nutrients.

We observed that certain species of mycelium be able to survive even in local condition, which could be the ideal species to grow in uncontrolled environment of Local.

Milky + Plurotus

Lentinus

The container is fully covered to avoid contamination during

The container is fully covered to avoid contamination during

Milky

Plurotus

The container is fully covered to avoid contamination during

The container is fully covered to avoid contamination during

Lentinus

Reishi

Plurotus Lentinus Milky

Milky

Plurotus

Experiment 2 -

Unit

Cover

Mycelium

Substrate

60% Coco coir 33% water 5% Pumice 2% Magnesium

Container

TOOLS PREPARATION

2 1

1. Alcohol 2. Aluminium Foil 3. Mixing Bowl 4. Gloves 5. Tweezers 6. Jars 7. Water 8. Digital Weight 9. Container 10. Substrate 11. Cooker 12. Pot

4

3

Sanitize and ute

5

7

6

8

9

10

11

Cover t withalu

12

Add su the con

e hands ensils

Mix the substrate with 50%water and tiny amount of nutrients

Stir it throughly

the jar uminium foil

Add water 1/3 of jar, and start boiling

Once temperature hits 65-85 Degree, turn off and leave it for hour

ubtrate to ntainer

Add mycelium into substrate

Close the lid, and leave it grows

EXPERIMENT CONTAINER 1

1. Plastic container 2. Cover 3. Holes

* The container covered with lid avoid risk of contamination * The container should have some holes for air exchnages * The container is transulcent allows just small amount of light to enter

AIR EXCHANGE

HUMIDNITY

No nutrients just leave it grow

Day 1

Day 2

Day 3

Day 4

Day 5

Day 6

Day 7

GL.01

LP.01

FV.01

TC.01

PO.01

SM.01

Sugar(glucose)+ Gypsum+ Pumice(pH)+ Magnesium

Day 1

Day 2

Day 3

Day 4

Day 5

Day 6

Day 7

GL.02

LP.02

FV.02

TC.02

PO.02

SM.02

Nutrients only Magnesium+Sugar

Day 1

Day 2

Day 3

Day 4

Day 5

Day 6

Day 7

GL.03

LP.03

FV.03

TC.03

PO.03

SM.03

Results

No nutrients just leave it grow

Sugar(glucose)+ Gypsum+ Pumice(pH)+ Magnesium

Nutrients only Magnesium+Sugar

GL.03

LP.03

FV.03

TC.03

PO.03

SM.03

Unit ; Conclusion

Oyster

Hard texture, form as a unit

This experiment help prove the fact that the external factor like nutrients and pH does not effect the growth of the mycelium. These are the 3 mycelium that seems to have potential in different aspect. As a result the first one have a hard texture, Second one has a spiky roots, and Third one is the softest, and the most fibrous.

Due to the fact that each mycelium have the different in it’s fibrous and texture, which these aspect will be affect on the ability of who will it take form.

Lentinus

Plurotus

Spiky roots

The softest, and the most fibrous

Experiment 3 -

Module

Cover

Mycelium

Substrate

60% Coco coir 33% water 5% Pumice 2% Magnesium

Container

Transfer to Shape When the bags are completely colonised, the mycelium is broken up again to be transferred to the desired shape. Once transferred,

Transfer to Shape When the bags are completely colonised, the mycelium is broken up again to be transferred to the desired shape. Once transferred,

Oyster Hard texture, form as a unit

Lentinus Spiky roots

Plurotus The softest, and the most fibrous

Plurotus The softest, and the most fibrous

How well mycelium take form Even each of modular focus on different functions, but the most important aspect of module is to be precise as possible.

The experiment test is to observe how well can different species of mycelium take shape in the mold. The quality to be observe such as, fine edge, small gaps, textures.

Experiment 4 -

System

Mycelium

Mycelium

Mycelium

1 -( 4.2 + 2.1 ) x 1.8 x 2 2 = 61.7 cm 3

1 -( 5.32 x 4.54 ) 2 = 60.328 cm 3

= 11.2 cm 2

= 4.1 cm

2

Structure

Surface Texture

System and Pattern These module system will be test on two things including, the system of each geometric, meaning the performant on how well it can take weight.

These threes system have the same number of volume.

Secondly, the qurity of each surface texture, how well it pervient sound.

The suface area will not be the same.

5x ( 2.5 x 5 ) = 62.5 cm

3

= 13.4 cm

2

Triangular Shape The triangular shape known as strong and stable shape to handle the loads

These is the layout of the mold *** Mold make from cardboard

Hexagonal Shape The surface of Hexagon shape designed to have a slope to form randomness of surfaces that could absorbs and reflect more sound

These is the layout of the mold *** Mold make from cardboard

Rectangular Shape The most simple and flexible shape that be able to form a complex geometry

These is the layout of the mold *** Mold make from cardboard

Hexagonal Shape

Triangular Shape

Weight This can be anything, however need to used the same in evey system Panel A panel to put the weight on top

Suface the weight transferred These will be different in each system geometric

The system of each Geometric Form

Rectangular Shape

A fungus, fungi or funguses is any member of a large group of eukaryotic organisms. A difficult species to fully identify, with over 80,000 named species worldwide and thought to be over a million yet to be identified. Most can only be identified by microscopic study of spores and flagella.These are the common species of mushroom in Thailand.

Hexagonal Shape

Triangular Shape

Microphone

Sound Source

A small condenser microphone which helps to "collect" sounds.

Using general mobile phone as sound source for portability and neutral input.

Panel A small condenser microphone which helps to "collect" sounds.

Closed Lid A lid cover this side of the box to prevent the sound to leak out of the box

Soundproof Qurity of each Surface Pattern

Rectangular Shape

Due to the surface texture of each system, it will directly affeact the soundproof quality. I f there is no texture on the surface we will not be able to test the soundproof only can text the load-bearing of the system There will be a panel between the box that cut into the shape of each system and at the end of one side(the side that provide the sound) will be a lid cover up and the other end will be where we place the mic (no lid)