LABELED YEAST EXTRACT INTERNAL STANDARD WORKFLOW KIT
The QC Workflow kit contains dilute-and-shoot uniquely labeled yeast extract for use as an internal standard in quantitative MS-based quantitative profiling studies. Yeast is an ideal internal standard because it contains 100s of key primary metabolites including sugars, coenzymes, nucleotides, organic acids, amino acids, peptides and polyamines.
Uses Targeted or untargeted, LC-MSbased analysis Ion suppression correction and sample normalization Accurate metabolite ID and quantitation Applicable to numerous sample matrices Method and instrument QC Biomarker discovery
Advantages of the unique 95% U-13C label Automated through the use of ClusterFinder software Labeled peaks easily found using software algorithms and their unlabeled counterparts in any sample matrix are identified and quantitated 100s of metabolite peaks in the yeast extract have been well characterized by LC-MS-MS and incorporated into ClusterFinder Improves metabolite identification Through the use of the 95% U-13C internal standards ClusterFinder can correct for ion suppression and normalize samples Built-in measurement of day-to-day (QA/QC) platform reproducibility Workflow and Software only $200 (90 samples)!
Summary of Benefits of the IROA 13Clabeled Yeast Extract Workflow 1. Daily instrument QA/QC. 2. Economical – $200 for 90 samples 3. No false data; removal of artifactual peaks. 4. Accurate compound formula ID for MS alone; complete ID with addition of SWATH, or IM. 5. Corrects for all source ionization losses for significantly better quantitation. 6. Normalizes samples to a universal standard for complete comparability. 7. Yeast metabolites have been characterized by several LC-MS methods and incorporated into ClusterFinder software. 8. ClusterFinder software solution builds libraries, IDs/quantitates compounds, corrects for ion-suppression and normalizes data. 9. Mass-spec vendor agnostic; specific extensions for inclusion of Sciex SWATH, and Agilent IM.
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