The Laboratory Report Fall 2015

The

Fall 2015

Laboratory Report A Publication of the Department of Pathology and Genomic Medicine

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Drs. Cagle, Chevez-Barrios, and Schwartz Named Texas Super Doctors

ongratulations to Drs. Philip Cagle, Patricia Chevez-Barrios, and Mary Schwartz on once again being voted to the list of Texas Super Doctors for 2015.

They have been selected for this honor many times over the past several years.

Dr. Cagle, medical director of pulmonary pathology for the Department of Pathology and Genomic Medicine is also Editor-in-Chief of Archives of Pathology and Laboratory Medicine, which has the largest circulation of any general pathology journal in the world and was voted the most influential pathology journal of the past 100 years by the Division of Biomedical and Life Sciences of the Special Libraries Association in 2009.

Table of Contents 2

PISA Conference Dr. Cagle Recieves CAP Award 3 New Cancer Tests from Molecular Diagnostics Dr. Powell on Resident Preparedness 4 Department Members in Peru 5 Department Welcomes New Faculty 6 Books by Department Members 7 CAP Highlights 8 New Residents and Fellows 9 OAD Welcomes New

Scientific Writer 10 Publications

Dr. Chevez-Barrios specializes in ophthalmic pathology and is the director of the ophthalmic pathology fellowship program for the Department. She is also the research co-director for the Retinoblastoma Center of Houston and the current president of the American Association of Ocular Oncologists and Pathologists. Dr. Schwartz is medical director of anatomic pathology and director of our surgical pathology fellowship. Dr. Schwartz is a recognized leader in pathology education and an invaluable resource to our clinicians.

ments in their respective field. The continued recognition of Drs. Cagle, Chevez-Barrios, and Schwartz as Super Doctors is indicative of their contributions to the profession of pathology and their dedication and hard work in the service of our patients and trainees. Congratulations once again!

Super Doctors are nominated by their peers for achieving high levels of recognition and attaining significant achieve-

Upcoming Conferences and Symposia

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Department Members Recognized at the Inaugural Conference of Pathobiology for Investigators, Students, and Academicians (PISA)

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he inaugural Pathobiology for Investigators, Students, and Academicians (PISA) conference, sponsored by the American Society for Investigative Pathology (ASIP), was held in Baltimore, Maryland on October 8-10. The conference “Pathways to Translational Medicine: Recent Advances in Cell Injury, Inflammation, and Neoplasia” was extremely well attended and the Houston Methodist Department of Pathology and Genomic Medicine was represented by several of our faculty and trainees.

Dr. Wesley Long, Associate Director of Diagnostic Microbiology, was awarded the ASIP Award for Junior Faculty for Outstanding Research in Experimental Pathology for his abstract titled: “Population Genomic Analysis of 1,201 Klebsiella pneumoniae Human Isolates Reveals Unexpectedly Extensive Diversity”. Other winners from the Department include Dr. Jianguo Wen, Assistant Research Professor of Pathology and

From left: Drs. Luchang Zhu, Wesley Long, and Jianguo Wen.

Genomic Medicine, for his abstract “Develop a New Aptamerdrug Conjugate to Target Multiple Myeloma”, and Dr. Luchang Zhu, a postdoctoral fellow in the Center for Molecular and Translational Human Infectious Diseases Research, for his abstract “A molecular trigger for intercontinental epidemics of group A Streptococcus”.

Houston Methodist Hospital and Research Institute will host the next PISA conference in Houston on October 20-22, 2016. The conference “Breakthroughs in Biology: From Underlying Pathogenesis to Translational Medicine” will be of interest to investigators and trainees from a spectrum of disciplines, and attendance is strongly encouraged. The website for next year’s conference is currently under development, but general information about PISA and this year’s meeting can be found at http://www.pisa2015.org/.

From left: Drs. Wesley Long, Randall Olsen, Luchang Zhu, Jesus Eraso, Huan Li, Youli Zu, and Jianguo Wen.

Dr. Cagle Receives the President’s Honor Award from the College of American Pathologists

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r. Philip Cagle has once again received a prestigious award from the College of American Pathologists (CAP) for his many contributions to the organization, the profession, and his patients. His most recent award, the President’s Honor Award, is presented by CAP President Dr. Gene Herbek to individuals who have gone above and beyond expectations this past year to assist CAP in meeting its goals. Dr. Cagle received the award at a private reception at CAP 2015 in Nashville in October.

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Next-Generation DNA Sequence Testing for Cancer Gene Mutations: Improving Patient Care

olecular diagnostics is a rapidly evolving part of laboratory medicine.

In particular, gene mutation testing in cancer has recently undergone dramatic changes. Until a few years ago, molecular assays testing a single cancer-associated gene, such as EGFR in lung cancer, were standard practice. However, our advanced understanding of cancer genetics now requires pathologists to test simultaneously each tumor for many gene mutations that confer key data on diagnosis, prognosis and predicted response to targeted therapy. Several cancer guideline documents published by the College of American Pathologists emphasize the need for pathologists to perform more biomarker testing. In response, the Houston Methodist Department of Pathology and Genomic Medicine’s Molecular Diagnostics Laboratory has implemented “Next-Generation Sequencing” technology, or NGS. Compared to traditional methods, NGS can rapidly and inexpensively test many genes using a very small amount of diagnostic tissue.

cancer associated genes, and the NGS76 breast cancer assay that can detect more than 4,000 mutations in 76 breast cancer-related genes. These NGS assays have been quickly embraced by oncologists throughout the Houston Methodist system, enhancing the care of many patients. Two illustrative examples of positive outcomes directly linked to our NGS assays are outlined below:

The molecular diagnostics team at Houston Methodist was an early adopter of NGS technology, and has extensively validated it in our clinical laboratory. We now offer two NGS assays, including the NGS-50 cancer assay that can detect approximately 2,800 mutations in 50

Case 2: A patient with a cancer of unknown origin presented with widespread metastasis, causing a treatment dilemma.

Without knowledge of the original cancer type, the most effective course of therapy was unknown. A generalized chemotherapy and radiation regimen was initiated.

Case 1: A patient with cancer that did not respond to standard chemotherapy was discussed in tumor board. The team was concerned that without a change in therapy, the tumor would rapidly grow and possibly metastasize. The NGS-50 cancer assay was performed, and identified a mutation that could be selectively targeted with a specific inhibitor drug.

Importantly, this result qualified the patient for multiple clinical trials available in the Houston area. The discovery of this gene mutation, made possible with the new NGS gene mutation test, led to a change in treatment and halted progression of disease.

When the disease continued to progress, a tumor of unknown origin test was performed at a reference laboratory, but the results were inconclusive. The NGS-50 cancer assay was then performed in our laboratory. In less than one week, we identified a targetable gene mutation.

With this new data, therapy was changed to include an agent that specifically targeted this mutation, resulting in a marked clinical response.

These cases illustrate the power of our NGS gene mutation assays to enhance patient care. Importantly, the results led to changes in therapy and improved outcomes. In the coming months, the molecular diagnostics laboratory will expand our NGS menu to include two new assays that detect the gene mutations most commonly affecting hematologic and endocrine malignancies. A hereditary breast cancer test is also being validated.

For more information contact: Molecular Diagnostics Laboratory 713-441-5727 Dr. Randall Olsen RJolsen@houstonmethodist.org Ms. Heather Hendrickson HLHendrickson@ houstonmethodist.org

Congratulations!

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ongratulations to Dr. Suzanne Powell on the publication of her very important article in the Archives of Pathology and Laboratory Medicine: “Employer Expectations for Newly Trained Pathologists: Report of a Survey From the Graduate Medical Education Committee of the College of American Pathologists”. The objective of the study, which surveyed fellows of the College of American Pathologists, was to determine which qualities and attributes of newly-trained pathologists were most valued by potential employers. This is a very informative study for both trainees and employers. You can read the full text article here.

Dr. Powell is Vice Chair of Education, Director of the AP/CP Residency Program, Co-Director of the Neuropathology Fellowship Program, and Chief of Neuropathology for the Department of Pathology and Genomic Medicine.

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Martin St-Amant - Wikipedia - CC-BY-SA-3.0

Department Members Help Underserved in Peru: CerviCusco

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everal members of the Department of Pathology and Genomic Medicine, including three faculty, three cytopathologists, two cytotechnologists, and a microbiologist volunteered their expertise to help diagnose and treat cervical cancer this past June in Cusco, Peru. The program, CerviCusco, is supported by the International Cervical Cancer (INCCA) Foundation and Medical College of Georgia, and its mission is to “improve the health and quality of life of women through the primary and secondary prevention of female genital tract cancers”. Cervical cancer is the number one cause of cancer deaths among women in Peru. Many women cannot afford Pap tests and most women already have advanced disease upon diagnosis. CerviCusco provides much needed tests to this underserved community in the Andean Highlands. The programs include vaccination against human papillomavirus (HPV, the causative agent of cervical cancer), physical exams and acute care for women, Pap tests, cervical, endocervical, and endometrial biopsies, and treatment. The group processed 1,500 liquid-based Pap tests and read ~2,000 slides during the week-long mission. Thank you to our dedicated department members who volunteered their time and expertise!

From left to right: Kay Arnold (Blythe Gorman’s aunt), Carla Deavers, Dr. Michael Deavers, Pat Cernoch, Erica Suarez, Debbie Smith, Christina Young, Dr. Blythe Gorman, Dr. Susan Haley, her husband Eric Mueller, and Nasera Hassan (an INCCA representative from Duke University).

Non-medical volunteers, such as family members, lend helping hands around the clinic.

Histology Gets New Automated Immunohistochemistry Instrument

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he Department of Pathology and Genomic Medicine recently acquired a new Leica Bond Automated Immunohistochemistry (IHC) instrument, the Leica Bond III. “It is a reliable platform that provides extra capacity and allows us to offer testing of additional antibodies that we were not able to do with the other autostainers,” says Dr. Michael Deavers, co-director of immunohistochemistry.

The new system processes samples 50% more rapidly than the previous instrument and is a great asset to the histology lab, which processes all IHC samples for hospitals within the Methodist Hospital System.

Pictured counter-clockwise from left: Drs. Stephen Shen and Michael Deavers, co-directors of IHC, and Candice Hamilton, IHC histotechnician specialist.

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Department Welcomes New Faculty

he Department of Pathology and Genomic Medicine welcomes four new faculty members. The department continues to grow to meet the increased patient volume resulting from Methodist’s ongoing expansion. Please extend a warm welcome to the following new faculty:

Matthew Cykowski, MD

Dr. Cykowski received his MD from the University of Texas Health Science Center (UTHSC) in San Antonio in 2003. He remained at UTHSC-San Antonio to complete a 1-year postdoctoral research fellowship in the Department of Cellular Biology and 3 years of postdoctoral training in the Research Imaging Center, and became a faculty associate in 2009. Dr. Cykowski completed his anatomic pathology residency at the University of Oklahoma Health Science Center where he served as the co-chief resident. He completed a neuropathology fellowship in the Department of Pathology and Genomic Medicine at Houston Methodist Hospital/MD Anderson Cancer Center in 2015, and was appointed as a staff pathologist in the Department of Pathology and Genomic Medicine here at Methodist. Dr. Cykowski’s research interests are focused on neurodegenerative disorders, including ALS, muscular dystrophy, and Alzheimer’s disease.

Mukul Divatia, MD

Dr. Divatia received his MBBS degree with honors from the Medical College Baroda in India in 2001. After completing his pathology residency there, he completed a fellowship in anatomic pathology at Tata Memorial Hospital in Mumbai, India, where he also held a position as a staff pathologist at Sterling Hospital. Dr. Divatia then completed a fellowship in ophthalmic pathology, and a residency in pathology and genomic medicine at Houston Methodist Hospital, followed by a fellowship in genitourinary pathology at Cedars Sinai Medical Center in Los Angeles. He is currently appointed at Houston Methodist hospital as a staff pathologist. His research interests include cancer and other diseases of the genitourinary tract.

S. Wesley Long, MD, PhD

Dr. Long received his MD degree in 2007 from the University of Texas Medical Branch (UTMB) in Galveston, where he also earned a PhD in experimental pathology. After finishing his doctoral studies, Dr. Long completed a clinical pathology residency at Houston Methodist Hospital. He is a staff pathologist and assistant medical director of the clinical microbiology laboratory in the Department of Pathology and Genomic Medicine. Dr. Long’s research interests center on the genetic underpinnings of multidrug-resistant bacteria and the identification of novel drug targets to treat highly-resistant strains.

Xin Yi, PhD

Dr. Yi completed her fellowship training in clinical chemistry at the University of Chicago School of Medicine after completing her PhD in clinical-bioanalytical chemistry at Cleveland State University in collaboration with Cleveland Clinic. Dr. Yi joined the department in September 2015 as associate medical director of clinical chemistry. She is a board-certified clinical chemist (DABCC) with broad expertise in clinical chemistry, endocrinology testing, therapeutic drug monitoring, toxicology, and clinical pharmacogenetics. She will direct mass spectrometry method development and the diagnostic immunology section of the laboratory. Her research interests include exploring potential applications of mass spectrometry in clinical laboratories, and studying pharmacogenomics using microarrays and next-generation sequencing tools.

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Books by Department Faculty The Atlas of Transplant Pathology, edited by Drs. Philip T. Cagle, Luan D. Truong, and Lisa Yerian, was recently published by the College of American Pathologists (CAP) Press. “In contrast to some other volumes that emphasize more of the underlying science and immunology, this really emphasizes the day-to-day routine diagnosis, and therefore is user-friendly and to the point,” says Philip T. Cagle, MD. Many members of our department (both current and former) contributed chapters. Authors include: Drs. Roberto Barrios, Yimin Ge, Hidehiro Takei, Lillian Gaber, Kevin Burns, Erin Consamus, Suzanne Crumley, Rachel Donohue, Abida Haque, Bryan Janssen, Geoffrey Land, Nathan Lee, Sergio Pina Oviedo, Jordan Roberts, and Haijun Zhou.

Congratulations to Drs. Andreana Rivera and Hidehiro Takei on the outstanding reception and review of their new book Advances in Surgical Pathology: Brain Cancer. The expert reviewer from Doody’s writes “The book assists practicing neuropathologists, surgical pathologists, and pathologists in training with the challenges of diagnosis… Neuro-oncologists, neurosurgeons, and basic scientists who want to update their understanding of all components of the diagnostic process also can benefit from this volume.” Well done Drs. Rivera and Takei!

Researchers Discover Genetic Basis for Epidemic Spread of Highly-Virulent Streptococci Investigators in the Department of Pathology and Genomic Medicine and the Center for Molecular and Translational Infectious Diseases Research have pinpointed the molecular events leading to a rapid increase of invasive infections in the early 1980’s caused by group A streptococcus (GAS), or the “flesh-eating disease”. By sequencing the genomes of over 3,600 strains from seven different countries, the group was able to identify only 3 single DNA substitutions that result in the over-production of two toxins that help the bacteria colonize and subsequently spread from person to person. These findings were made possible through advances in next generation sequencing technology, which allows simultaneous high-throughput sequencing of hundreds of isolates at a modest cost. The full article is available online. A brief, 5-minute video of Dr. Musser and the research team explaining their findings is available on the JCI website.

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Department Awards Three New Microgrants

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he Department of Pathology and Genomic Medicine awarded three new microgrants to faculty investigators. The Department’s microgrant program is designed to fund innovative proposals that will lead to an expansion of our clinical service. The review committee considers several criteria in its assessment, such as clinical need, likelihood of a significant return on investment, involvement of residents and/or fellows and multiple faculty, and likelihood of leading to a peer-reviewed publication.

Congratulations to the following recipients: Michael Thrall, MD; Chunyan Liu, MD, PhD; Heather Hendrickson, MT(ASCP); Kumar Krishan, MD; Randall Olsen, MD, PhD; and Shannon Butler-Williams, MD, for their proposal “Next generation sequencing as an adjunct for bile duct cytology interpretation”.

Yimin Ge, MD; Chunyan Liu, MD, PhD; Dina Mody, MD; and Mary Schwartz, MD, for their proposal “Contributing factors for negative hrHPV tests in women with histology-confirmed high-grade squamous intraepithelial lesions (HSIL)”.

Yimin Ge, MD; Eric Bernicker, MD; David Bernard, MD, PhD; Philip Cagle, MD; and Chunyan Liu, MD, PhD, for their proposal “Evaluation of cell-free plasma tumor DNA (ptDNA) in molecular characterization of lung adenocarcinoma”.

CAP 2015 Highlights: Poster Presentations by Department Trainees

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everal Department of Pathology and Genomic Medicine trainees presented posters at the College of American Pathologists annual meeting held October 4-7 in Nashville, Tennessee.

Congratulations to the following trainees and their faculty mentors: “Elevated Fecal Calprotectin Levels Predict Active Inflammation on Lower Gastrointestinal Tract Biopsies” by Jordan A. Roberts, MD; David Cohen, MD; and Mary R. Schwartz, MD. “ALK-Positive Large Cell Lymphoma With Unusual Morphology and Immunophenotype” by Wei Xie, MD, PhD; Arthur Zieske, MD; Yuval Raizen, MD; Bryce Portier, MD, PhD; Youli Zu, MD, PhD; and Suyang Hao, MD. “Stepping Beyond the Paraffin Curtain: Attitudes and Experience on Error Disclosure Among Pathologists and Trainees” by David A. Cohen, MD; Suzanne Z. Powell, MD; and Timothy C. Allen, MD, JD. “Tibia Osteoma Presenting as a Radiopaque, Well-Circumscribed Medullary Cavity Lesion”

by Paul Christensen, MD, and Alberto Ayala, MD. “Ovarian Calcified Thecoma With Extensive Adipose Metaplasia: A Rare Entity in a Young Patient” by Ya Xu, MD, PhD; Clifford Sauls; MD; and Michael Deavers, MD.

Dr. David Cohen Elected to CAP Resident Forum

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r. David Cohen, a PGY3 resident in the Department of Pathology and Genomic Medicine was elected as Secretary to the College of American Pathologists Resident Forum Executive Committee.

Congratulations Dr. Cohen!

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Department Welcomes 2015-2016 Residents and Fellows Residents Ghadah Al Sannaa, MD University of Dammam College of Medicine Saudi Arabia

Andreia Barbieri, MD Louisiana State University School of Medicine Shreveport

Daniel Duhon, MD Louisiana State University School of Medicine Shreveport

Ziad El-Zaatari, MD American University of Beirut Faculty of Medicine

G. Eli Morey, MD, MPH University of Vermont College of Medicine

Alejandro Perez, MD Vanderbilt University College of Medicine

Fellows Blood Bank

Clinical Chemistry Eric Salazar, MD, PhD Chief Fellow PhD: Pharmacology, Weill Cornell Graduate School of Medical Sciences and Rockefeller University, 2010 MD: Weill Cornell Medical College, 2012 Residency: Houston Methodist Hospital, 2015

Cytopathology

Clayton Wilburn, MD MD: Vanderbilt University, 2012 Residency: University of Texas Medical Branch, 2015

Hematopathology Shannon Butler-Williams, MD MD: St. George’s University School of Medicine, Grenada, 2011 Residency: Medical University of South Carolina, 2015

Rachel Donohue, MD MD: University of New Mexico School of Medicine, 2011 Residency: Houston Methodist Hospital, 2015

Steven Goodman, MD MD: Sackler School of Medicine, Tel Aviv, Israel, 2010 Residency: UMass Memorial Medical Center, 2014 Fellowship: Surgical Pathology, MD Anderson Cancer Center, 2015

Vidya Nagrale, MD MD: Seth GS Medical College and King Edward Memorial Hospital, Mumbai, India, 2004 Residency: University of Illinois Hospital and Health Sciences System, 2014

Molecular Genetics Chunyan Liu, MD, PhD MD: China Medical University, 1988 PhD: Shinshu University School of Medicine, Nagano, Japan, 1999 Residency: Creighton University School of Medicine, 2015

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Neuropathology Leomar Ballester, MD, PhD PhD: Vanderbilt University, 2007 MD: University of Puerto Rico School of Medicine, 2011 Residency: National Cancer Institute, 2014 Fellowship: Molecular Genetic Pathology, Baylor College of Medicine, 2015

Surgical Pathology

Andrea Gilbert, DO Medical School: Kentucky College of Osteopathic Medicine, 2011 Residency: Virginia Commonwealth University Health System, 2015

Troy Davis, MD MD: University of Louisville, 2010 Residency: University of Louisville, 2014 Fellowship: Blood Bank/Transfusion Medicine, University of Louisville, 2015

Ross Miller, MD MD: Sanford School of Medicine, University of South Dakota, 2010 Residency: Sanford School of Medicine, University of South Dakota, 2014 Fellowship: Cytopathology, Houston Methodist Hospital, 2015

Jariel Hairston, MD MD: University of Mississippi School of Medicine, 2011 Residency: University of Mississippi Medical Center, 2015

Nicole Nelles, MD Medical School: University of Texas Medical Branch, 2010 Residency: Houston Methodist Hospital, 2014 Fellowship: MD Anderson Cancer Center, 2015

WELCOME!

Jana Wimmer, MD MD: University of Texas Medical Branch, 2010 Residency: Houston Methodist Hospital, 2014 Fellowship: Cytopathology, MD Anderson Cancer Center, 2015

New Scientific Writer Joins the Department The Office of Academic Development (OAD) is pleased to announce that Dr. Helen Chifotides has joined the office in the role of scientific writer. Dr. Chifotides is an accomplished scientific writer and editor–in leading and managerial roles–with over 12 years of experience. She has authored 2 book chapters and 32 high profile, highly-cited publications, including 3 review articles, which are published in top-tier peer-reviewed scientific journals – with over 2,000 citations to date. Dr. Chifotides received her BS and PhD in Chemistry from the National and Kapodistrian University of Athens, Greece, and was appointed as a senior biochemist in the biochemistry laboratory of the General Hospital for Chest Diseases in Athens. She then became a member of the Chemistry Department at Texas A&M University as a senior research associate and most recently, an assistant research scientist. Her research studies focused on the interactions of anticancer metal complexes with DNA and other biomolecules. She is a member of the American Medical Writers Association, a board-certified Editor in the Life Sciences (ELS), and is completing her Advanced American Medical Writers Association certificate.

The OAD provides editorial services to the Department’s trainees and faculty, assists with the identification and procurement of research funding, helps in the writing and submission of IRB protocols, and offers training in grantsmanship and scientific writing. For more information on the OAD services or if you need assistance, please contact Dr. Chifotides (HChifotides@houstonmethodist.org) or Dr. Kathryn Stockbauer (KStockbauer@houstonmethodist.org).

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Recent Publications Chaikriangkrai K, Valderrabano M, Bala SK, Alchalabi S, Graviss EA, Nabi F, Mahmarian J, Chang SM. Prevalence and Implications of Subclinical Coronary Artery Disease in Patients With Atrial Fibrillation. Am J Cardiol. 2015 Oct15;116(8):1219-23. PMID: 26279110.

Zhu L, Olsen RJ, Nasser W, de la Riva Morales I, Musser JM. Trading Capsule for Increased Cytotoxin Production: Contribution to Virulence of a Newly Emerged Clade of emm89 Streptococcus pyogenes. MBio. 2015 Oct 6;6(5). PMID: 26443457.

Driver BR, Portier BP, Mody DR, Deavers M, Bernicker EH, Kim MP, Teh BS, Santacruz JF, Kopas L, Munden RF, Cagle PT. NextGeneration Sequencing of a Cohort of Pulmonary Large Cell Carcinomas Reclassified by World Health Organization 2015 Criteria.

Arch Pathol Lab Med. 2015 Oct 2. PMID: 26430808.

Post MD, Johnson K, Brissette MD, Conran RM, Domen RE, Hoffman RD, McCloskey, CB, Raciti PM, Roberts CA, Rojiani AM, Tucker JA, Powell SZ. Employer Expectations for Newly Trained Pathologists: Report of a Survey From the Graduate Medical Education Committee of the College of American Pathologists. Arch Pathol Lab Med. 2015 Oct 2. PMID: 26430810.

Mishra DK, Compean SD, Thrall MJ, Liu X, Massarelli E, Kurie JM, Kim MP. Human Lung Fibroblasts Inhibit Non-Small Cell Lung Cancer Metastasis in Ex Vivo 4D Model. Ann Thorac Surg. 2015 Oct;100(4):1167-74. PMID: 26233278.

Olsen RJ, Raghuram A, Cantu C, Harrell-Hartman M, Jimenez F, Lee S, Ngo A, Rice KA, Saddington D, Spillman H, Valson C, Flores AR, Beres SB, Long SW, Nasser W, Musser JM. The Majority of 9,729 Group A Streptococcus Strains Causing Disease Secrete SpeB Cysteine Protease: Pathogenesis Implications. Infect Immun. 2015 Sep 28. PMID: 26416912.

Kanamori M, Suzuki H, Takei H, Sonoda Y, Uenohara H, Tominaga T. Malignant transformation of diffuse astrocytoma to glioblastoma associated with newly developed BRAF V600E mutation. Brain Tumor Pathol. 2015 Sep 24. PMID: 26404554.

Chen J, Phillips B, Chandler WL. Evaluation of prothrombin time and activated artial thromboplastin time mixing studies using an estimated factor correction method. Blood Coagul Fibrinolysis. 2015 Sep 19. PMID: 26397883.

Brat DJ, Cagle PT, Dillon DA, Hattab EM, McLendon RE, Miller MA, Buckner JC; Members of the Cancer Biomarker Reporting Committee, College of American Pathologists. Template for Reporting Results of Biomarker Testing of Specimens From Patients With Tumors of the Central Nervous System. Arch Pathol Lab Med. 2015 Sep;139(9):1087-93. PMID: 25642959.

Cachia D, Kamiya-Matsuoka C, Mandel JJ, Olar A, Cykowski MD, Armstrong TS, Fuller GN, Gilbert MR, De Groot JF. Primary and secondary gliosarcomas: clinical, molecular and survival characteristics. J Neurooncol. 2015 Sep 9. PMID: 26354773.

Huang L, Lou Y, Ju H, Zhang L, Pan JS, Ross A, Sun Y, Truong LD, Sheikh-Hamad D. Severe Nephrotoxic Nephritis following Conditional and Kidney-Specific Knockdown of Stanniocalcin-1. PLoS One. 2015 Sep 22;10(9):e0138440. PMID: 26393521.

Strickland BA, Cachia D, Jalali A, Cykowski MD, Penas-Prado M, Langford LA, Li, J, Shah K, Weinberg JS. Spinal Anaplastic Oligodendroglioma With Oligodendrogliomatosis: Molecular Markers and Management: Case Report. Neurosurgery. 2015 PMID: 26352098.

Jeong IG, Dajani D, Verghese M, Hwang J, Cho YM, Hong JH, Kim CS, Ahn H, Ro JY. Differences in the aggressiveness of prostate cancer among Korean, Caucasian, and African American men: A retrospective cohort study of radical prostatectomy. Urol Oncol.

2015 Sep 4. PubMed PMID: 26345648.

Flores AR, Jewell BE, Yelamanchili D, Olsen RJ, Musser JM. A Single Amino Acid Replacement in the Sensor Kinase LiaS Contributes to a Carrier Phenotype in Group A Streptococcus. Infect Immun. 2015 Nov;83(11):4237-46. PMID: 26283331.

Huang M, Barber SM, Moisi M, Powell S, Rivera A, Zwillman M, Rose J. Cervical Epidural Hematoma after Chiropractic Spinal Manipulation Therapy in a Patient with an Undiagnosed Cervical Spinal Arteriovenous Malformation. Cureus. 2015 Aug 18;7(8):e307.

PMID: 26430581.

Chaikriangkrai K, Jhun HY, Graviss EA, Jyothula S. Overweight-mortality paradox and impact of six-minute walk distance in lung transplantation. Ann Thorac Med. 2015 Jul-Sep;10(3):169-75. PMID: 26229558.

McClain CM, Deavers MT, Sauls CD, Diwan AH. Hidradenoma papilliferum associated with pregnancy: a case report. J Cutan Pathol. 2015 Aug 12. PMID: 26265265.

Park IH, Kong SY, Ro JY, Kwon Y, Kang JH, Mo HJ, Jung SY, Lee S, Lee KS, Kang HS, Lee E, Joo J, Ro J. Prognostic Implications of Tumor-Infiltrating Lymphocytes in Association With Programmed Death Ligand 1 Expression in Early-Stage Breast Cancer. Clin Breast Cancer. 2015 Aug 6. PMID: 26364145.

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Recent Publications (continued) Takei H, Rouah E, Bhattacharjee MB. Cerebellar pleomorphic xanthoastrocytoma in a patient with neurofibromatosis type 1: a case report and literature review. Int J Clin Exp Pathol. 2015 Jun 1;8(6):7570-4. eCollection 2015. PMID: 26261671 Abudayyeh A, Truong LD, Beck LH Jr, Weber DM, Rezvani K, Abdelrahim M. Membranous nephropathy in autologous hematopoietic stem cell transplant: autologous graft-versus-host disease or autoimmunity induction? Clin Kidney J. 2015 Aug;8(4):440-4.

PMID:26251713.

Wen J, Tao W, Hao S, Iyer SP, Zu Y. A unique aptamer-drug conjugate for targeted therapy of multiple myeloma. Leukemia. 2015 Aug 5. PMID: 26242462.

Kim K, Sung CO, Park BH, Ku JY, Go H, Ro JY, Kim J, Cho YM. Immunoprofile-based subgrouping of urothelial bladder carcinomas for survival prediction. Hum Pathol. 2015 Oct;46(10):1464-70. PMID: 26232864.

Ghebremariam YT, Cooke JP, Gerhart W, Griego C, Brower JB, Doyle-Eisele M, Moeller BC, Zhou Q, Ho L, de Andrade J, Raghu G, Peterson L, Rivera A, Rosen GD. Pleiotropic effect of the proton pump inhibitor esomeprazole leading to suppression of lung inflammation and fibrosis. J Transl Med. 2015 Aug 1;13:249. PMID: 26231702.

Truong LD, Trostel J, Garcia GE. Absence of nicotinic acetylcholine receptor alpha-7 subunit amplifies inflammation and accelerates onset of fibrosis: an inflammatory kidney model. FASEB J. 2015 Aug;29(8):3558-70. PMID: 25985801.

Roberts JA, ChĂŠvez-Barrios P. Levamisole-Induced Vasculitis: A Characteristic Cutaneous Vasculitis Associated With LevamisoleAdulterated Cocaine. Arch Pathol Lab Med. 2015 Aug;139(8):1058-61. PMID: 26230600.

Zhao N, Pei SN, Qi J, Zeng Z, Iyer SP, Lin P, Tung CH, Zu Y. Oligonucleotide aptamer-drug conjugates for targeted therapy of acute myeloid leukemia. Biomaterials. 2015 Oct;67:42-51. PMID: 26204224.

Zhang Y, Wu M, Han X, Wang P, Qin L. High-Throughput, Label-Free Isolation of Cancer Stem Cells on the Basis of Cell Adhesion Capacity. Angew Chem Int Ed Engl. 2015 Sep 7;54(37):10838-42. PMID: 26190051.

Sun H, Zu Y. A Highlight of Recent Advances in Aptamer Technology and Its Application. Molecules. 2015 Jun 30;20(7):11959-80.

PMID: 26133761.

Mai KT, Truong LD, Ball CG, Williams P, Flood TA, Belanger EC. Invasive urothelial carcinoma exhibiting basal cell immunohistochemical markers: A variant of urothelial carcinoma associated with aggressive features. Pathol Res Pract. 2015 Aug;211(8):610-8. PMID: 26100813.

Tiwari N, Powell SZ, Takei H. Recurrent subependymoma of fourth ventricle with unusual atypical histological features: A case report.

Pathol Int. 2015 Aug;65(8):438-42. PMID: 26059172.

Miller RA, Waters LL, Mody DR, Tams KC. Squamous cell carcinoma of the cervix: a cytology-histology-human papillomavirus correlation in clinical practice. Arch Pathol Lab Med. 2015 Jun;139(6):776-81. PMID: 26030246.

Cykowski MD, Coon EA, Powell SZ, Jenkins SM, Benarroch EE, Low PA, Schmeichel AM, Parisi JE. Expanding the spectrum of neuronal pathology in multiple system atrophy. Brain. 2015 Aug;138(Pt 8):2293-309. PMID: 25981961.

Choi EK, Kim D, Divatia M, Takei H. Molecular alterations of appendiceal serrated lesions. Hum Pathol. 2015 Jul;46(7):1062-3.

PMID: 25934250.

Takei H, Rouah E, Barrios R. Intravascular carcinomatosis of central nervous system due to metastatic inflammatory breast cancer: A case report. Neuropathology. 2015 Oct;35(5):456-61. PMID: 25923831.

Raphael I, Nalawade S, Eagar TN, Forsthuber TG. T cell subsets and their signature cytokines in autoimmune and inflammatory diseases. Cytokine. 2015 Jul;74(1):5-17. PMID: 25458968.

Musser JM, DeLeo FR. Molecular pathogenesis lessons from the world of infectious diseases research. Am J Pathol. 2015 Jun;185(6):1502-4. PMID: 25906759.

Lyons YA, Kamat AA, Zhou H, Mody DR, Schwartz MR, Hobday C, Ge Y. Non-16/18 high-risk HPV infection predicts disease persistence and progression in women with an initial interpretation of LSIL. Cancer Cytopathol. 2015 Jul;123(7):435-42. PMID: 25903015.

Cao Z, Kaleta E, Wang P. Simultaneous Quantitation of 78 Drugs and Metabolites in Urine with a Dilute-And-Shoot LC-MS-MS Assay.

J Anal Toxicol. 2015. Jun;39(5):335-46. PMID: 25833899.

THE LABORATORY REPORT Fall 2015

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Recent Publications (continued) Sanson M, Makthal N, Gavagan M, Cantu C, Olsen RJ, Musser JM, Kumaraswami M. Phosphorylation events in the multiple gene regulator of group A Streptococcus significantly influence global gene expression and virulence. Infect Immun. 2015 83(6):238295. PMID: 25824840.

Kim J, Coffey DM, Ma L, Matzuk MM. The ovary is an alternative site of origin for high-grade serous ovarian cancer in mice. Endocrinology. 2015 Jun;156(6):1975-81. doi: 10.1210/en.2014-1977. PMID:25815421.

Fiskus W, Sharma S, Saha S, Shah B, Devaraj SG, Sun B, Horrigan S, Leveque C, Zu Y, Iyer S, Bhalla KN. Pre-clinical efficacy of combined therapy with novel beta-catenin antagonist BC2059 and histone deacetylase inhibitor against AML cells. Leukemia.

2015 Jun;29(6):1267-78. PMID: 25482131.

THE LABORATORY REPORT is a publication of the Houston Methodist Department of Pathology and Genomic Medicine Editor-in-Chief April Ewton, MD

Editorial Committee Hazel Awalt, MD Christopher Leveque, MD Claudia Molina, MD Seema Mullick, MD Thu Ngo, MD

Steven Shen, MD, PhD Ping Wang, PhD

Department Chair James M. Musser, MD, PhD

Editorial Coordinator Kathryn Stockbauer, PhD

Houston Methodist Hospital Roland Cruikshank, MBA Manuel Hinojosa, MHA