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ADVANCED CELLULAR AND TISSUE MICROSCOPY CORE

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Texas Children’s Neurological Research Institute

M.D. Anderson Cancer Center

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Texas Children’s Texas Hospital Heart St. Luke’s Institute Hospital

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Houston Methodist

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Manager: Kemi Cui, M.D., Ph.D. Email: kcui@houstonmethodist.org 713.441.7270

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Advanced Cellular and Tissue Microscopy Core

The Core provides Houston Methodist and the community access to advanced high-performance microscopy for cell biology and tissue pathology studies. The facility specializes in various forms of wide field imaging, high resolution laser scanning confocal microscopies, various living cell imaging systems, and two high content screening systems (ImagestreamX and ImageXpress).

EQUIPMENT & RESOURCES • I magestreamX Imaging Cytometer High Content Screening System - 1,000 cells per second - 6 image channels per cell: SSC, brightfield, fluorescent - Up to 5 lasers (488, 405, 561, 592, 658 nm) - Multiple magnifications (60X/.9NA, 40X/.75NA, 20X/.5NA) • ImageXpress Micro High Content Screening System - Automated microscopy imaging solutions for 100+ most common assays - Captures > 10 million cells/hour in a low-resolution; 1 million cells / hour in high resolution - Transmitted light, environmental control for live cell imaging - Robotic fluidics handling capability • Live Cell Spinning Disk Confocal Imaging System - F  ully motorized disk control, easy switching between confocal and wide-field - Excellent for live-cell imaging with fast acquisition and minimal phototoxicity - EM-CCD camera, full frame (512X512) up to 15 frames/ second • FluoView™ FV1000 Confocal Microscope - High resolution confocal imaging of live cells and fixed tissues - SRV CCD camera and CO2/humidity/temperature control - Phase contrast, DIC, 4X, 10X, 20X, 40X, 40X, 60X & 100X - IR (748) laser is available

• I ncuCyte™ Live-Cell Imaging---A Microscope within an incubator - Ideal for critical time-lapse images of live cells - A network-accessible interface and automatic focuses - Supporting hundreds of vessels (plates, flasks, dishes and slides) • Total Internal Reflection Fluorescence Microscopy - A key technique in the study of molecular interactions near cell surface - Two lasers (488, mArgon and 543 HeNe), EM-CCD camera and 100X (NA 1.49) - Environmental-controlled by Tokai Hit Stage Top incubator • Nikon A1 Confocal Imaging System - High-quality images at high speed and enhanced sensitivity - High resolution images up to 4096 X 4096 pixels - Fast acquisition of 32-channel spectral images • Laser Capture Micro-Dissection (LCM, Arcturus) - Ideal for micro-dissection of single cells - Micro-dissection of cells expressing fluorescent protein (GFP) - Combine LCM and UV laser cutting for flexibility • Inverted/Upright Fluorescent Microscopes - 2X, 4X, 10X, 20X, 40X, 60X and 100X, bright-field and phase contrast - 100X dark field imaging - Fluorescence (DAPI, FITC, TRITC, Texas Red and Cy5) • CRI Nuance Multispectral Imaging System - A powerful multispectral imaging system - Multiplexed labeling while retaining intact morphology

Advanced Cellular & Tissue Microscopy Core  
Advanced Cellular & Tissue Microscopy Core