ReferenceCultureNewVersion

*protective agent: 10 % skim milk plus 1 % monosodium glutamate

Protective media

Application

Most bacteria

Yeasts

Fungi

Liquid drying

Composition

Sodium L(+)-glutamate monohydrate 3 g.

Ribitol 1.5 g., L-cysteine hydrochloride monohydrate 0.05 g.

0.1M potassium phosphate buffer (pH 0.7) 100 ml.

A: Polyvinylpyrrolidone (K-90) 6 g., Lactose 5 g., Distilled water 75 ml.

B: Sodium L(+)-glutamate monohydrate 3 g.

1M potassium phosphate buffer (pH 0.7) 10 ml., Distilled water 15 ml.

Sterilized separately by autoclaving, then mix A&B.

Sodium L(+)-glutamate monohydrate 3 g.

0.1M potassium phosphate buffer (pH 0.7) 100 ml

1% Activated charcoal added in protective media for lactic acid bacterial 5% Trehalose added in protective media for yeasts

Quality control of freeze-drying and liquid drying ampoules

Freezing (sporulating fungi, yeasts and bacteria)

Freezing (non-sporulating filamentous fungi)

Reviving frozed cultures

Cross streak for purification check

Preparation of back up, stock and working cultures