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Detection (qualitative) methods
Enumeration (quantitative) methods
Confirmation and typing methods
Identification methods
Sensitivity
Level of detection (relative level of detection)
Inclusivity and exclusivity
Relative trueness
Accuracy profile
Inclusively and exclusively
Inclusively and exclusively
Identification accuracy Note:
Premises (clause 4)
Validation and verification (clause 17)
Control microorganisms:
internal and external quality control (clauses 14 and 16
Test and confirmation methods (clauses 10 to 13)
Valid Test Results
Personnel (clause 5)
Equipment and consumables including calibration and checks, cleaning, disinfection, sterilization (clauses 6, 7)
Preparation and use of culture media (clause 8)
Laboratory samples (clause 9)


Micro wells
1 2.5 ml of A. but 0.5 ml removed 2.0 ml (of 0.1 g/ml)
2 0.5 ml from Row 1 into 2.0 ml diluent, mixed and 0.5 ml removed 2.0 ml (of 0.02 g/ml)
3
ml from Row 2 into 2.0 ml diluent, mixed and 0.5 ml removed
4 0.5 ml from Row 3 into 2.0 ml diluent, mixed 2.5 ml (of 0.0008 g/ml)

Article info
ISO 7218:2024: Microbiology of the food chain - General requirements and guidance for microbiological examinations
A guide to using the ISO MPN Calculators: ISO - International Organization for Standardization











