Uses of Double Beam Spectrophotometer

USES OF DOUBLE BEAM SPECTROPHOTOMETER

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About Us A UV spectrophotometer is used to determine the absorption of light from a sample and can be used as a detector for HPLC. A sample is placed in the UV/VIS beam and absorbance versus wavelength is measured. A double beam spectrophotometer utilizes two beams of light: a reference beam and a sampling beam that passes through the sample.

Some Double Beam UV spectrophotometer have two detectors that allow the two beams to be measured at one time.

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Device This device employs two light beams, thus the name “double beam” spectrophotometer www.igenels.com

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Reference beam: To measure lamp energy, this beam passes through the reference standard.

Sample beam: To reflect sample to reflect sample absorption.

Applications and Uses of Double Beam Spectrophotometers 01

DNA ANALYSIS

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PROTEIN ANALYSIS

WAVELENGTH SCANNING

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KINETICS

QUANTITATIVE ANALYSIS

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Key Operating Principles

ABSORBANCE

DISPERSION OF LIGHT RAYS

THE VISIBLE SPECTRUM SCATTERING OF LIGHT RAYS www.igenels.com

Effective Analysis For effective analysis of the diverse biological specimens, the double beam UV/visible spectrophotometer is used.

Measurements of samples are also performed using the electromagnetic spectrum. The processes of dispersion, reflection, and refraction all occur in spectrophotometry instruments, which further aids in the taking of analytical data.

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