USES OF DOUBLE BEAM SPECTROPHOTOMETER
www.igenels.com
About Us A UV spectrophotometer is used to determine the absorption of light from a sample and can be used as a detector for HPLC. A sample is placed in the UV/VIS beam and absorbance versus wavelength is measured. A double beam spectrophotometer utilizes two beams of light: a reference beam and a sampling beam that passes through the sample. Some Double Beam UV spectrophotometer have two detectors that allow the two beams to be measured at one time.
www.igenels.com
Device This device employs two light beams, thus the name “double beam” spectrophotometer www.igenels.com
01
02
Reference beam: To measure lamp energy, this beam passes through the reference standard.
Sample beam: To reflect sample to reflect sample absorption.
Applications and Uses of Double Beam Spectrophotometers 01
DNA ANALYSIS
04
02
PROTEIN ANALYSIS
WAVELENGTH SCANNING
05
03
KINETICS
QUANTITATIVE ANALYSIS
www.igenels.com
Key Operating Principles
ABSORBANCE
DISPERSION OF LIGHT RAYS
THE VISIBLE SPECTRUM SCATTERING OF LIGHT RAYS www.igenels.com
Effective Analysis For effective analysis of the diverse biological specimens, the double beam UV/visible spectrophotometer is used. Measurements of samples are also performed using the electromagnetic spectrum. The processes of dispersion, reflection, and refraction all occur in spectrophotometry instruments, which further aids in the taking of analytical data.
How To Find Us
IGENE LABSERVE 16/2 | Ground Floor, Ashok Nagar | New Delhi - 110018 09310696848 info@igenels.com