nutriFORUM 2019 - El reto de la producción y las soluciones probióticas

Production challenges and probiotic solutions Wouter Van der Veken Global Product Manager Probiotics

Overview 1.

Current situation

2.

Production diseases a) b)

Necrotic enteritis Dysbacteriosis

3.

Probiotic solutions

4.

Conclusion

Current situation – Production diseases – Probiotic solutions – Conclusion

Current situation Challenges 

Production diseases



AB reduction



Consumer demands



Animal welfare



…

Current situation – Production diseases – Probiotic solutions – Conclusion

Production diseases “Diseases which tend to persist in animal production systems and, typically, become more prevalent or severe, in proportion to the potential productivity of the system”

Current situation – Production diseases – Probiotic solutions – Conclusion

Production diseases Impact 10-15% reduced performance efficiency on average Uncontrolled Controlled

Economic losses per bird due to clostridiosis in broiler flocks

Current situation – Production diseases – Probiotic solutions – Conclusion

Necrotic enteritis Definition Severe necrosis of intestinal mucosa

Causative agent Clostridium perfringens

Consequences  Susceptible to secondary infections

 Increased FCR

 Decreased health

 Decreased final weights

Current situation – Production diseases – Probiotic solutions – Conclusion

Dysbacteriosis Definition Inflammation of small intestine/disruption of normal gut flora

Cause Stress leading to feed passage alteration, creating conditions for Clostridium perfringens proliferation

Consequences  Disrupted gut integrity

 Impaired digestion

 Decreased health and performance

 Bacterial overgrowth

Current situation – Production diseases – Probiotic solutions – Conclusion

Clostridium perfringens 

Gram-positive



Rod-shaped



Anaerobic



Spore-forming



Pathogenic



Ever-present in nature

Type A toxin: understanding changed!

Current situation – Production diseases – Probiotic solutions – Conclusion

Probiotic solutions Definition Beneficial viable micro-organisms incorporated into feed or drinking water to enhance the gut and its microbiota, resulting in a health and/or performance benefit Origin Human application - yoghurt, kefir, fermented food, …

Antibiotic reduction - need for alternatives

Current situation – Production diseases – Probiotic solutions – Conclusion

Probiotic solutions Importance gut health Production diseases, but also… 

Performance



Wet litter



Lameness (Bacterial chondronecrosis + osteomyelitis)



…

Current situation – Production diseases – Probiotic solutions – Conclusion

Probiotic solutions

Current situation – Production diseases – Probiotic solutions – Conclusion

Probiotic solutions Potential

Current situation – Production diseases – Probiotic solutions – Conclusion

Probiotic mode of action 1. Competition for nutrients 2. Bioconversion 3. Production of growth substrates

4. Direct antagonism 5. Competitive exclusion 6. Improved barrier function 7. Reduction of inflammation

8. Stimulation immune response Which probiotic to choose?

Bermudez-Brito et al, 2012

Adjusted www.customprobiotics.com

Current situation – Production diseases – Probiotic solutions – Conclusion

Bacillus licheniformis 

Gram-positive



Spore-forming



Facultative anaerobic



Production of metabolites

Lichenicidin

Germinated state (A, B) and spore (C) Vogel et al., 2008

Current situation – Production diseases – Probiotic solutions – Conclusion

Lichenicidin Bacillus licheniformis Formation of lichenicidin 



Antimicrobial peptide

B. licheniformis inhibition zone vs. C. perfringens Angels et al., 2018



Nanomolar activity



No known resistance: targets lipid II in cell walls

Targeted inhibition of C. perfringens Begley et al. (2009), Caetano et al. (2011), Dischinger et al. (2009)

Current situation – Production diseases – Probiotic solutions – Conclusion

Lichenicidin – in vitro

Inhibition of undesirable bacteria

Current situation – Production diseases – Probiotic solutions – Conclusion

Lichenicidin – in vivo Set-up Control

B-Act®

Commercial farm, Germany: 23 300 turkeys divided over 2 houses (1 per group) Groups 1. Control

2. 1.6 x 1012 CFU B. licheniformis / mton of feed (B-Act®)

Internal research

Current situation – Production diseases – Probiotic solutions – Conclusion

Probiotics and necrotic enteritis Performance under NE infection (0- 28 days) 1000

Set-up 2.2

900

360 broilers (Cobb 500) per group

2.1b

946a 839b

838b

Infection challenge

2

1. 5000 oocysts E. Maxima/bird (day 13)

800 1.8

700

1.6a

1.7a 1.7a

1.6

600 1.4

500 1.2

400

300

1

non-infected birds

infected birds + BMD 50 ppm

Internal research - different letters indicate significant differences (P<0.05)

infected birds

infected birds + BAct 0.5 kg

FCR

WG (kg)

709c

2. 108 CFU C. perfringens (day 18, 19 and 20)

Groups 1.

2.

3.

4.

uninfected group infected group infected group + 50 ppm BMD infected group + 1.6 x 1012 CFU B. licheniformis / mton of feed (B-Act®)

Current situation – Production diseases – Probiotic solutions – Conclusion

Probiotics and necrotic enteritis Mortality under NE infection (0-28 days) 18

Set-up

17b

360 broilers (Cobb 500) per group

16

Infection challenge

percentage mortality (%)

14

1. 5000 oocysts E. Maxima/bird (day 13)

12

2. 108 CFU C. perfringens (day 18, 19 and 20)

10

Groups

8 6 4

2a

2

0

0a

0a

non-infected birds

infected birds + BMD 50 ppm

Internal research - different letters indicate significant differences (P<0.05)

infected birds

infected birds + B-Act 0.5 kg

1.

2.

3.

4.

uninfected group infected group infected group + 50 ppm BMD infected group + 1.6 x 1012 CFU B. licheniformis / mton of feed (B-Act®)

Current situation – Production diseases – Probiotic solutions – Conclusion

Probiotics and dysbacteriosis Set-up

1000 broilers (Ross 308) Groups

1. Control 2. 1.6 x 1012 CFU B. licheniformis / mton of feed (B-Act®) Scoring 50 birds/group were scored for dysbacteriosis Results

Dysbacteriosis score

Control

B-Act®

Day 24

1.24

0.57

Day 31

1.13

0.48

Internal research

Strong decrease of dysbacteriosis lesions

Current situation – Production diseases – Probiotic solutions – Conclusion

Chosing the right probiotic

Dong et al., 2017

Current situation – Production diseases – Probiotic solutions – Conclusion

Chosing the right probiotic 1.

Single-strain or multi-strain Inter-product competition

Current situation – Production diseases – Probiotic solutions – Conclusion

Chosing the right probiotic 1.

Single-strain or multi-strain Inter-product competition

2.

Spores or vegetative cells Stability

Sample ID

Recovery of cells after pelleting (85°C, 30s)

Lactobacillus

<1 %

B. licheniformis

± 100 %

Internal research

Current situation – Production diseases – Probiotic solutions – Conclusion

Chosing the right probiotic 1.

Single-strain or multi-strain Inter-product competition

2.

Spores or vegetative cells Stability

3.

Coloniser or non-coloniser Extra requirement + difficult “The inability of probiotic cultures to establish permanent colonization in the gastrointestinal tract has been well documented (Jacobsen et al., 1999; Tannock et al., 2000; Frese et al., 2012). “ – Spivey et al., 2014  continued repeated administration is required

Current situation – Production diseases – Probiotic solutions – Conclusion

Chosing the right probiotic 1.

Single-strain or multi-strain Inter-product competition

2.

Spores or vegetative cells Stability

3.

Coloniser or non-coloniser Extra requirement + difficult

4.

Feed component combinations Safety + added benefit

Current situation – Production diseases – Probiotic solutions – Conclusion

Chosing the right probiotic 1.

Single-strain or multi-strain Inter-product competition

2.

Spores or vegetative cells Stability

3.

Coloniser or non-coloniser Extra requirement + difficult

4.

Feed component combinations Safety + added benefit

5.

Information Minimal assumptions

Current situation – Production diseases – Probiotic solutions – Conclusion

Chosing the right probiotic 1.

Single-strain or multi-strain

2.

Spores or vegetative cells

3.

Coloniser or non-coloniser

4.

Feed component combinations

5.

Information

Current situation – Production diseases – Probiotic solutions – Conclusion

Conclusion 1.

Production diseases related to gut health 

Necrotic enteritis



Dysbacteriosis



Secondary challenges

2.

Need for profitable AB reduction

3.

Difficult, saturated probiotic market 

Make the right choice

Thank you!

Wouter Van der Veken Global Product Manager Probiotics wouter.vanderveken@huvepharma.com

A special thanks to all authors of the used content â&#x20AC;&#x201C; references available upon request