The availability of new technology vaccines has been increased worldwide since the mid 2000’s, as well as their widespread use within the poultry industry in the past 5 years, especially at hatchery level. Therefore, an updated review, with a practical and visual approach, has been thoroughly developed in order to highlight the importance of this type of vaccination in poultry farming, whatever the vaccination technique used (in ovo, subcutaneous or spray vaccination), to control the main diseases affecting this species (infectious bursal disease, Newcastle disease, infectious laryngotracheitis, etc.). The handbook has been written by a prestigious expert with a wide experience in this field. Nume-rous graphic resources (images, graphs, tables, flow charts) have been included to complement the information provided and make the con-tents more understandable and accessible to readers.