Total Protein Extraction by TRIzol Extraction of DNA, RNA and proteins is a common procedure involving in molecular approaches for the studies of genome, transcriptome and proteome of biological samples. Although designed for the extraction of DNA, RNA and proteins, TRIzol is utilized most frequently for the extraction of nucleic acids, while RIPA is the most widely used agent for protein extraction. Simultaneous extraction of DNA, RNA, and proteins is sample-saving, and coordinated analysis of an individual sample is crucial for accurate correlations between genomic aberrations and their consequences on the transcriptome and proteome. Thus, we provide a detailed protocol describing extraction of total protein following TRIzol isolation of nucleic acids. Reagents: TRIzol Chloroform Isopropanol Ethanol PBS: To prepare 1 L 1X PBS: dissolve 8 g of NaCl, 0.2 g of KCl, 1.44 g of Na2HPO4, 0.24 g of KH2PO4 in 800 mL distilled H2O, then adjust pH to 7.4 with HCl and adjust volume to 1 L with additional distilled H2O. 0.3 M guanidine hydrochloride solution: To prepare 100 mL: dissolve 2.87 g guanidine hydrochloride in 80 mL 95% ethanol, and adjust volume to 100 mL with additional 95% ethanol. 1% SDS solution: To prepare 100 mL: dissolve 1 g SDS in 100 mL distilled H2O.
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