The guide "Manual for Participatory Food System Sustainability Assessments and Transformation" offers an introduction to multidimensional assessment of food systems, based on experiences from action research carried out by scientists and practitioners from the Centre for Development and Environment (CDE), University of Bern, Switzerland, together with their partners in food systems in Africa and South America. It describes how to conduct analyses in participatory ways, how to assess the degree to which food systems are sustainable and democratic, and how interested food system actors can engage in co-designing and implementing “transformative actions” for food sustainability and democracy. The manual includes examples and illustrations from the experiences in practice.