Hydrophobic Interaction Chromatography (HIC)

ADC drugs are covalently coupled with hydrophobic drug linkers, toxins, monoclonal antibodies, and impurities that reveal different drug-to-antibody ratios, and hydrophobic chromatography is used according to the hydrophobic differences for removal.

Journal of Pharmaceutical and Biomedical Analysis. 2020, 186: 113313.

• Small Molecule Impurities

• Host DNA Impurities

• Endotoxin Impurities

• Charge Variant Impurities

Ion Exchange Chromatography (IEC)

Ion exchange chromatography involves protein analysis, such as charge variant analysis and glycoform analysis, to elucidate the structural changes of proteins. Ion exchange chromatography generally yields high recovery and removes non-antibody-related compounds, including small molecules and endotoxins. Therefore, ion exchange chromatography is a promising method of ADC purification.

Hydroxyapatite Chromatography (HA)

Hydroxyapatite chromatography is a useful method for monoclonal antibody purification.

The Crystalline Structure of HA

Hydroxyapatite chromatography utilizes calcium and phosphate minerals, which bind to antibodies through the interaction of calcium metal affinity and phosphate-based cation exchange.

 The use of hydroxyapatite chromatography can reduce antibody aggregates from 60% to 0.1%.