HALO ES-CN

NEW! HALO ES-CN

•Moderately polar phase for alternate selectivity •Increased retention of polar compounds •Decreased retention of hydrophobic compounds

•Steric-Protected silane for low pH stability •Fully end-capped for neutral pH applications

Separation of mixed EPA explosive standards 8330 A and B on HALO C18 and ES-CN Phases Conditions: Columns: 4.6 x 100 mm, HALO C18, HALO ES-CN Mobile phase: 50/50:Methanol/water Flow: 1.25 mL/min; Pressure: about 300 bar Temperature: 30 deg. C. 4

Peak identities: 1) HMX, 2) RDX, 3) 1,3,5-TNB, 4) 1,3-DNT, 5) nitrobenzene, 6) Tetryl, 7) 2,4,6-TNT, 8) 4-amino-2,6,-DNT 9) 2-amino-4,6-DNT, 10) 2,6-DNT, 11) 2,4-DNT 12) 2-nitrotoluene, 13) 4-nitrotoluene, 14) 3-nitrotoluene

3 10, 11 1

5

Absorbance @ 254 nm

2

6

7

9 8 12

HALO C18

5

4, 3

12

13 14

7

10

2

3

9

8 2

1

14

11

HALO ES-CN

0

13

4 Minutes

6 1

5

6

7

8

Selectivity ES-CN vs. C18 Columns: 4.6 x 100mm Mobile phase: 40/60-water/MeOH for C18 50/50-water/MeOH for ES-CN Flow rate: 1.25 mL/min Pressure: 310 Bar Temperature: 30 째C.

6

Peaks: 1) resourcinol 2) benzyl alcohol 3) phenylacetonitrile 4) 1-indanol 5) 3,4-dinitrotoluene 6) 2,3-dinitrotoluene

1 5

3 2

7

Absorbance @ 254 nm

HALO C18

8

9

7) 2,4-dinitrotoluene 8) anisole 9) 1-chloro4-nitrobenzene 10) toluene i = impurity

4 i

10

i

1 3

2 HALO ES-CN

8

1

5

7

4 10

i 0

6 9

2

3 Minutes

4

5

6