Tube Type Acrylic Restrainers.. ...... ....cont.

Move stopper

 toward distal end of tube until mouse has just enough space to breathe comfortably without being able to move around

4. CHEMICAL RESTRAINT

4.1 Anesthesia

 Anaesthesia can be achieved by an intramuscular injection of the following:

 Mice: Ketamine and xylazine.

 Injection sites are the large muscle masses in the hind limbs.

 Inhalational anaesthesia (Halothane or isofluorane) may be used.

 Animals should be placed in an induction chamber at 3-5% in oxygen at 1L/min, and then maintained at 1-2% by face mask.

 Mice can be safely restrained by grasping firmly at the base of the tail.

 This form of restraint is suitable for moving the mouse over a short distance, animal identification and weighing.

 For greater control such as during examination, or injections, place the mouse on a surface it can grasp.

 A suitable surface is a wire cage top or a towel.

 Apply a slight tension to the base of the tail so the mouse grasps onto the surface.

 Gently but firmly place your free hand over the shoulders and quickly grasp the scruff of the neck close to the base of the skull between the thumb and forefinger.

 Restrain the tail by your little finger.

References

 https://theodora.com/rodent_laboratory/restraint.html

 https://web.jhu.edu/animalcare/procedures/restraint.html

 www.orrchoward.com/working instructions for mouse restraint technique

 RES002 RESTRAINT AND HANDLING OF PEST ANIMALS USED IN RESEARCH ,

Prepared by Trudy Sharp, Glen Saunders & Bruce Mitchell