OUR TECHNOLOGY
Introducing the MiMode™ PuraBead® range of mixed-mode resins, designed to deliver smart ligand chemistry for complex challenges. By combining multiple binding interactions within tailored chemistries, MiMode™ resins provide advanced separation power for proteins, viral vectors, nucleic acids, and other biologics. With Astrea Bioseparations’ 30+ years of expertise and proven manufacturing quality, the MiMode™ family offers flexibility from discovery to GMP production, ensuring robust, reproducible results every step of the way.
The MiMode™ range
MiMode™ PuraBead® resins offer a step-change in purification capability. Designed for use with diverse feedstocks and varying pH or salt conditions, MiMode™ offers exceptional versatility for multiple applications. Available in three chemistries—hydrophobic, anion, and cation mixed-mode binding modes—each resin is engineered for chemical stability and reusability, ensuring consistent performance and cost-effectiveness throughout development. With formats ranging from 96-well plates, RoboColumns®, pre-packed SNAP® and Evolve® columns to bulk resin, MiMode™ enables seamless scalability from rapid screening through to manufacturing-scale processes.
Hydrophobic mixed-mode resins
The MiMode™ PuraBead® hydrophobic range harnesses both hydrophobic and hydrogen-bonding interactions to target proteins and viral particles where other approaches often fail. The hydrophobic family includes MiMode™ PuraBead® HX1, HX2, HL3, and HL4 resins, each tuned to offer slightly different binding profiles, allowing developers to identify the optimal solution for their molecule. For high-throughput evaluation, the MiMode™ PuraBead® Mixed-H 96-well plate provides rapid, efficient screening in a simple, smart, and effective format, compatible with both manual pipetting and automated platforms.
Key features
• Harness hydrophobic and hydrogen-bonding interactions for enhanced selectivity
• Four resin options (MiMode™ PuraBead® HX1, HX2, HL3, HL4) with distinct binding profiles
• Effective against challenging targets such as aggregates, viral particles, and host-cell impurities
• Available in multiple formats
Advantages
• Resolves targets where traditional ion exchange methods fail
• Provides fine-tuned selectivity across protein classes and viral vectors
• High-throughput screening with Mixed-H 96-well plate enables rapid condition scouting
• Simplifies downstream purification by reducing polishing steps
Anion-exchange mixed-mode resin
MiMode™ anion exchange resin combine ionic and hydrophobic interactions to provide strong selectivity for negatively charged biomolecules. These resins excel in applications such as nucleic acid clearance, viral purification, and polishing of therapeutic proteins. The mixed-mode design ensures a balance between binding strength and elution control, even in the presence of complex feed streams or high salt environments. By offering higher resolution than conventional ion exchangers, MiMode™ anion exchange resins enable cleaner purification strategies with fewer steps, reducing timelines while maintaining process robustness.
Key features
• MiMode™ PuraBead® AX1 combines anion exchange and hydrophobic interactions for improved impurity clearance
• Optimized for negatively charged biomolecules such as nucleic acids and viral particles
• Designed to balance strong binding interaction with controlled elution conditions
• Broad compatibility with different feed streams and buffer systems
• Available in multiple formats
Advantages
• Delivers superior DNA and HCP removal compared to conventional AEX resins
• Supports virus purification and nucleic acid clearance in gene therapy workflows
• Reduces process steps by multiple-binding mechanisms
• Maintains robust performance across a wide pH and conductivity range
Commitment to quality
Astrea Bioseparations is honored to support the biopharmaceutical industry with products and services that help bring life-saving and improving medicines to market. Regardless of process scale, we are ready to build Purity by Design into each process to ensure quality and speed to market.
Cation-exchange mixed-mode resins
MiMode™ cation exchange resins are designed for powerful purification of positively charged biomolecules, including monoclonal antibodies, enzymes, and proteins with isoelectric points above neutral. By combining ionic binding with hydrophobic and hydrogen-bonding interaction contributions, these resins provide enhanced flexibility in binding and release, supporting both capture and polishing steps in bioprocess workflows. Their unique selectivity improves impurity removal while maintaining high recovery. With scalable formats, MiMode™ cation exchangers simplify development and enable consistent performance at every stage.
Key features
• MiMode™ PuraBead® CBX1 and CBX2 combine a unique mix of cation, hydrophobic, boronate ester, and hydrogen-bonding interactions binding
• MiMode™ PuraBead® CB1 and CB2 combine cation, hydrophobic, and hydrogen-binding interactions
• Ideal for capture and polishing in applications such as monoclonal antibodies, enzymes, and other positively charged proteins
• Available in high-throughput and process-scale formats for seamless scale-up
Advantages
• Uniquely made for proteins with pI above neutral
• Enables efficient purification of biomolecules from complex feedstocks
• Reduces process steps through multiple binding mechanisms
Technical support
Astrea Bioseparations provides expert technical support to help optimize purification strategies. Our chromatography specialists are available to assist with process development, troubleshooting, and scalability considerations.
OUR RANGE
Screening formats
Early-stage process development requires rapid evaluation of multiple resins under parallel conditions. MiMode™ screening formats provide a complete toolkit for resin selection and optimization. The 96-well plate format is pre-packed with MiMode™ resins and available as single-resin or mixed-resin configurations, enabling fast, high-throughput screening with minimal sample use. For automation, RoboColumns® provide consistent miniaturized formats compatible with leading robotic liquid handling platforms. To support scale-up, MiMode™ resins are also available in 1 mL and 5 mL pre-packed SNAP® columns for convenient method development. At process scale, Evolve® process and Evolve®D columns offer GMP-ready, pre-packed solutions with the same reliability and performance.
Order information
Hydrophobic mixed-mode resins Code
FG00628-00025
FG00628-00100
FG00628-00500
FG00628-01000
FG00628-00025
3904-00100
3904-00500
3904-01000
3906-00025
3906-00100
3906-00500
3906-01000
3260-00025
3260-00100
3260-00500
3260-01000
Manufacture & supply
Adsorbent batches are produced in Astrea Bioseparations’ controlled environment manufacturing facility under an ISO 9001 quality system. Astrea Bioseparations offers a range of formats, from small scale to single-use, process-ready, pre-packed Evolve®D columns, packed with your resin for a ready-to-go manufacturing solution.
Regulatory support
Astrea Bioseparations can provide customers with comprehensive regulatory support files for adsorbents manufactured and can support customers with regulatory filings for their processes.
MiMode™ PuraBead® HX1
MiMode PuraBead® HX1
MiMode™ PuraBead® HX1
MiMode PuraBead® HX1
MiMode™ PuraBead® HX2
MiMode PuraBead® HX2
MiMode™ PuraBead® HX2
MiMode™ PuraBead® HX2
MiMode™ PuraBead® HL3
MiMode™ PuraBead® HL3
MiMode™ PuraBead® HL3
MiMode™ PuraBead® HL3
MiMode™ PuraBead® HL4
MiMode™ PuraBead® HL4
MiMode™ PuraBead® HL4
MiMode™ PuraBead® HL4
Anion-exchange mixed-mode resins
FG00634-00025
FG00634-00100
PuraBead® AX1
PuraBead® AX1
FG00634-00500 MiMode™ PuraBead® AX1
FG00634-01000
Cation-exchange mixed-mode resins
MiMode™ PuraBead® AX1
FG00683-00025
FG00683-00100
FG00683-00500
FG00640-00025
FG00640-00100
FG00640-00500
FG00640-01000
FG00677-00025
FG00677-00100
FG00677-00500
FG00677-01000
MiMode™ PuraBead® CBX2
MiMode™ PuraBead® CBX2
MiMode™ PuraBead® CBX2
PuraBead® CX1
MiMode™ PuraBead® CX1
MiMode™ PuraBead® CX1
MiMode™ PuraBead® CX1 1
MiMode™ PuraBead® CX2
MiMode™ PuraBead® CX2
MiMode™ PuraBead® CX2
MiMode™ PuraBead® CX2
Astrea Bioseparations also supplies larger volumes of bulk resins & pre-packed chromatography columns for cGMP development and manufacturing-scale processes.
For more information on other available formats or any other matters, please visit our website or contact us at sales@astrea-bio.com.
