PuraBead HF ®
Ion Exchange Adsorbents
www.astreabioseparations.com
2 | PuraBead® HF Ion Exchange Adsorbents
OUR TECHNOLOGY Ion exchange (IEX) chromatography is used to separate proteins based on their net charge at a particular pH. A protein is positively charged when the pH is below the pI of the protein and is negatively charged when the pH is above the pI of the protein. Astrea Bioseparations has developed four high performance anion & cation exchange adsorbents which have broad applicability: DEAE PuraBead® HF (weak anion exchanger); Q PuraBead® HF (strong anion exchanger); CM PuraBead® HF (weak cation exchanger) and SP PuraBead® HF (strong cation exchanger). These adsorbents are designed for capture, intermediate purification and polishing steps - engineered for use in demanding process applications.
Key Features
Astrea Bioseparations’ ion exchange chromatography adsorbents have excellent flow properties and high binding capacities. SP PuraBead® HF is intended for use in downstream process capture steps but can also be used for polishing applications. Q, DEAE and CM PuraBead® HF have been designed for use in capture, intermediate purification and polishing applications.
• Stable in all commonly used buffers and solutions
• Uniform particle size • Excellent flow properties (up to 600 cm/hr; up to 1 bar) • Good resolution & high binding capacities • Cost effective & reliable • Easy to clean (robust, long life adsorbents)
Advantages • In house manufactured agarose beads (PuraBead® 6HF) minimising lead times • PuraBead® 6HF base matrix manufactured using a solvent free process • Easy to pack (typical asymmetries of 0.8 – 1.2) • Suitable for cleaning with 1.0 M NaOH • Performance maintained over 100s of cycles • Autoclavable if needed (in 0.1 M saline, 121 °C for 30 minutes)
Astrea Bioseparations’ proprietary PuraBead® 6HF base matrix technology with a near mono-disperse particle size distribution which provides high resolution, reduces the risk of column fouling and renders the adsorbents easy to pack.
PuraBead® HF Ion Exchange Adsorbents | 3
PRODUCT FORMATS Bulk Manufacture & Supply
Pack Size
CM PuraBead® HF
SP PuraBead® HF
Astrea Bioseparations supplies bulk adsorbents for developmental and regulated cGMP manufacturing applications in volumes sufficient to meet the requirements of the largest biomanufacturing processes.
25 mL
3450-00025
3453-00025
100 mL
3450-00100
3453-00100
500 mL
3450-00500
3453-00500
Our Ion exchange adsorbents are manufactured in Astrea Bioseparations’ controlled environment manufacturing facility under an accredited ISO 9001 quality system and are supported by comprehensive regulatory support files.
1L
3450-01000
3453-01000
Ordering Information
Astrea Bioseparations also offer a range of pre-packed columns including 1 mL and 5 mL column kits and a range of other larger pre-packed column solutions.
Adsorbent Gel Slurry
For small orders up to 500 mL, please visit our webshop.
Pack Size
CM PuraBead® HF
SP PuraBead® HF
We also offer a range of larger pack sizes for supply of bulk resins into cGMP development and manufacturing scale processes.
6606
CM PuraBead® HF 1 mL Column Kit
4 x 1 mL columns
6607
CM PuraBead® HF 1 mL Column Kit
4 x 5 mL columns
6604
SP PuraBead® HF 1 mL Column Kit
4 x 1 mL columns
6605
SP PuraBead® HF 1 mL Column Kit
4 x 5 mL columns
6602
DEAE PuraBead® HF 1 mL Column Kit
4 x 1 mL columns
6603
DEAE PuraBead® HF 1 mL Column Kit
4 x 5 mL columns
6600
Q PuraBead® HF 1 mL Column Kit
4 x 1 mL columns
6601
Q PuraBead® HF 1 mL Column Kit
4 x 5 mL columns
Pack Size
DEAE PuraBead® HF
Q PuraBead® HF
25 mL
3451-00025
3452-00025
100 mL
3451-00100
3452-00100
500 mL
3451-00500
3452-00500
1L
3451-01000
3452-01000
Commitment to Quality
Technical Support
Astrea Bioseparations is honored to support the biopharmaceutical industry with products and services that help bring life-saving and improving medicines to market. Regardless of process scale, we are ready to build Purity by Design into each process to ensure quality and speed to market.
Astrea Bioseparations is a world leader in adsorbent manufacture and chromatography development and can provide full support and in-house training for the development of new applications and their implementation for biopharmaceutical manufacture.
+44 (0) 1223 433 800 | astreabioseparations.com sales@astrea-bio.com | techsupport@astrea-bio.com | quality@astrea-bio.com Global bases in North America, Canada and Cambridge UK HQ: Horizon Park, Barton Road, Comberton, Cambridge, CB23 7AJ, UK
IEx Purabead HF - V1 This product is covered by or for use under one or more patents: www.astreabioseparations.com/patents All trademarks, trade names, trade dress, product names and logos appearing in this brochure are the property of Astrea UK Services Ltd. Copyright © 2022 Astrea Bioseparations Ltd. All rights reserved.