PuraBead HF ®
HIC Adsorbents
www.astreabioseparations.com
2 | PuraBead® HF HIC Adsorbents
OUR TECHNOLOGY Hydrophobic Interaction Chromatography (HIC) is used to separate proteins and biomolecules based on their hydrophobic nature. Samples are applied to the HIC adsorbent in buffers containing high concentration of salt and eluted with a salt gradient. Consequently, HIC is particularly useful for purifying proteins from high ionic strength feedstocks. Astrea Bioseparations Ltd and its affiliates (Astrea Bioseparations) manufacture HIC adsorbents comprising of a range of four ligands of increasing hydrophobicity; Butyl, Hexyl, Octyl, and Phenyl coupled to our highly cross-linked near monodisperse 6% beaded agarose (PuraBead® 6HF) which gives the adsorbents excellent flow properties. These HIC adsorbents are designed for capture or intermediate purification steps for the purification of mildly hydrophobic biomolecules and are engineered for use in demanding process applications. Our four high performance HIC resins are Butyl Purabead® HF, Hexyl Purabead® HF, Octyl Purabead® HF and Phenyl Purabead® HF. These adsorbents have different levels of hydrophobicity determined by their ligand structures, ligand densities and hydrocarbon chain length. Best practice when evaluating the adsorbents is to screen all four HIC’s and identify which of the products is most suited to your process or application.
Our proprietary PuraBead® 6HF base matrix technology with a near mono-disperse particle size distribution which provides high resolution, reduces the risk of column fouling and renders the adsorbents easy to pack.
Key Features • Good resolution & high binding capacities • Cost effective & reliable • Uniform particle size • Excellent flow properties (up to 600 cm/hr; up to 1 bar) • Suitable for use in all common buffers and solutions • Suitable for cleaning with 1.0 M NaOH
Advantages • PuraBead® 6HF agarose base matrix manufactured using a solvent free process • In house manufactured PuraBead® 6HF minimising lead timess • Easy to pack (typical asymmetries of 0.8 – 1.2) • Robust and stable adsorbents • Performance maintained over 100s of cycles • Autoclavable if needed (in 0.1 M saline, 121 °C for 30 minutes)
The adsorbents are compatible with most biological buffers and commonly encountered buffer additives and are stable in up to 1.0 M sodium hydroxide which allows for stringent cleaning and sanitization protocols.
PuraBead® HF HIC Adsorbents | 3
PRODUCT FORMATS Bulk Manufacture & Supply
Pre-Packed Columns
Astrea Bioseparations supplies bulk adsorbents for developmental and regulated cGMP manufacturing applications in volumes sufficient to meet the requirements of the largest biomanufacturing processes. Our HIC adsorbents are manufactured in Astrea Bioseparations’ controlled environment manufacturing facility under an accredited ISO 9001 quality system with regulatory support files available on request.
Ordering Information Our HIC adsorbents are available to order online or via sales@astrea-bio.com using the information below.
Adsorbent Gel Slurry Pack Size
Phenyl PuraBead® HF
Butyl PuraBead® HF
25 mL
3408-00025
3416-00025
100 mL
3408-00100
3416-00100
500 mL
3408-00500
3416-00500
1L
3408-01000
3416-01000
Pack Size
Hexyl PuraBead® HF
Octyl PuraBead® HF
25 mL
3426-00025
3455-00025
100 mL
3426-00100
3455-00100
500 mL
3426-00500
3455-00500
1L
3426-01000
3455-01000
Product code
Description
Pack size
6610
Phenyl PuraBead® HF 1 mL Column Kit
4 x 1 mL columns
6611
Phenyl PuraBead® HF 5 mL Column Kit
4 x 5 mL columns
6634
Butyl PuraBead® HF 1 mL Column Kit
4 x 1 mL columns
6635
Butyl PuraBead® HF 5 mL Column Kit
4 x 5 mL columns
6636
Hexyl PuraBead® HF 1 mL Column Kit
4 x 1 mL columns
6637
Hexyl PuraBead® HF 5 mL Column Kit
4 x 5 mL columns
6612
Octyl PuraBead® HF 1 mL Column Kit
4 x 1 mL columns
6613
Octyl PuraBead® HF 5 mL Column Kit
4 x 5 mL columns
HIC selection kit (1 mL columns)
1 x 1 mL Phenyl PuraBead® HF 1 x 1 mL Butyl PuraBead® HF 1 x 1 mL Hexyl PuraBead® HF 1 x 1 mL Octyl PuraBead® HF
HIC selection kit (5 mL columns)
1 x 5 mL Phenyl PuraBead® HF 1 x 5 mL Butyl PuraBead® HF 1 x 5 mL Hexyl PuraBead® HF 1 x 5 mL Octyl PuraBead® HF
6614
6615
Single Columns Product code
Description
6710
Phenyl PuraBead HF Column
1 x 1 mL
Robocolumns
6712
Octyl PuraBead® HF Column
1 x 1 mL
Product code
Description
1 x 1 mL
4520-PC3408
Phenyl PuraBead HF, 200 µL
1 row of 8
Hexyl PuraBead® HF, 200 µL
1 row of 8
6734
Pack size ®
Butyl PuraBead® HF Column
Pack size ®
6736
Hexyl PuraBead® HF Column
1 x 1 mL
4520-PC3426
6711
Phenyl PuraBead® HF Column
1 x 5 mL
4520-PC3455
Octyl PuraBead® HF, 200 µL
1 row of 8
6713
Octyl PuraBead® HF Column
1 x 5 mL
4520-PC3416
Butyl PuraBead® HF, 200 µL
1 row of 8
6735
Butyl PuraBead® HF Column
1 x 5 mL
6737
Hexyl PuraBead HF Column
1 x 5 mL
®
+44 (0) 1223 433 800 | astreabioseparations.com sales@astrea-bio.com | techsupport@astrea-bio.com | quality@astrea-bio.com Global bases in North America, Canada and Cambridge UK HQ: Horizon Park, Barton Road, Comberton, Cambridge, CB23 7AJ, UK
HIC Purabead® HF - V2 This product is covered by or for use under one or more patents: astreabioseparations.com/patents All trademarks, trade names, trade dress, product names and logos appearing on this document are the property of Astrea UK Services Limited or their respective owners. RoboColumns are packed by, and RoboColumn® is a trademark of Repligen GmbH. Copyright © 2023 Astrea Bioseparations Ltd. All rights reserved.