HIC Brochure

PuraBead HF ®

HIC Adsorbents

www.astreabioseparations.com

2 | PuraBead® HF HIC Adsorbents

OUR TECHNOLOGY Hydrophobic Interaction Chromatography (HIC) is used to separate proteins and biomolecules based on their hydrophobic nature. Samples are applied to the HIC adsorbent in buffers containing high concentration of salt and eluted with a salt gradient. Consequently, HIC is particularly useful for purifying proteins from high ionic strength feedstocks.

Astrea Bioseparations Ltd and its affiliates (Astrea Bioseparations) manufacture HIC adsorbents comprising of a range of four ligands of increasing hydrophobicity; Butyl, Hexyl, Octyl, and Phenyl coupled to our highly cross-linked near monodisperse 6% beaded agarose (PuraBead® 6HF) which gives the adsorbents excellent flow properties. These HIC adsorbents are designed for capture or intermediate purification steps for the purification of mildly hydrophobic biomolecules and are engineered for use in demanding process applications.

Our four high performance HIC resins are Butyl Purabead® HF, Hexyl Purabead® HF, Octyl Purabead® HF and Phenyl Purabead® HF. These adsorbents have different levels of hydrophobicity determined by their ligand structures, ligand densities and hydrocarbon chain length. Best practice when evaluating the adsorbents is to screen all four HIC’s and identify which of the products is most suited to your process or application.

Our proprietary PuraBead® 6HF base matrix technology with a near mono-disperse particle size distribution which provides high resolution, reduces the risk of column fouling and renders the adsorbents easy to pack.

Key Features • Good resolution & high binding capacities • Cost effective & reliable • Uniform particle size • Excellent flow properties (up to 600 cm/hr; up to 1 bar) • Suitable for use in all common buffers and solutions • Suitable for cleaning with 1.0 M NaOH

Advantages • PuraBead® 6HF agarose base matrix manufactured using a solvent free process • In house manufactured PuraBead® 6HF minimising lead timess • Easy to pack (typical asymmetries of 0.8 – 1.2) • Robust and stable adsorbents • Performance maintained over 100s of cycles • Autoclavable if needed (in 0.1 M saline, 121 °C for 30 minutes)

The adsorbents are compatible with most biological buffers and commonly encountered buffer additives and are stable in up to 1.0 M sodium hydroxide which allows for stringent cleaning and sanitization protocols.

PuraBead® HF HIC Adsorbents | 3

PRODUCT FORMATS Bulk Manufacture & Supply

Pre-Packed Columns

Astrea Bioseparations supplies bulk adsorbents for developmental and regulated cGMP manufacturing applications in volumes sufficient to meet the requirements of the largest biomanufacturing processes.

Our HIC adsorbents are manufactured in Astrea Bioseparations’ controlled environment manufacturing facility under an accredited ISO 9001 quality system with regulatory support files available on request.

Ordering Information Our HIC adsorbents are available to order online or via sales@astrea-bio.com using the information below.

Adsorbent Gel Slurry Pack Size

Phenyl PuraBead® HF

Butyl PuraBead® HF

25 mL

3408-00025

3416-00025

100 mL

3408-00100

3416-00100

500 mL

3408-00500

3416-00500

1L

3408-01000

3416-01000

Pack Size

Hexyl PuraBead® HF

Octyl PuraBead® HF

25 mL

3426-00025

3455-00025

100 mL

3426-00100

3455-00100

500 mL

3426-00500

3455-00500

1L

3426-01000

3455-01000

Product code

Description

Pack size

6610

Phenyl PuraBead® HF 1 mL Column Kit

4 x 1 mL columns

6611

Phenyl PuraBead® HF 5 mL Column Kit

4 x 5 mL columns

6634

Butyl PuraBead® HF 1 mL Column Kit

4 x 1 mL columns

6635

Butyl PuraBead® HF 5 mL Column Kit

4 x 5 mL columns

6636

Hexyl PuraBead® HF 1 mL Column Kit

4 x 1 mL columns

6637

Hexyl PuraBead® HF 5 mL Column Kit

4 x 5 mL columns

6612

Octyl PuraBead® HF 1 mL Column Kit

4 x 1 mL columns

6613

Octyl PuraBead® HF 5 mL Column Kit

4 x 5 mL columns

HIC selection kit (1 mL columns)

1 x 1 mL Phenyl PuraBead® HF 1 x 1 mL Butyl PuraBead® HF 1 x 1 mL Hexyl PuraBead® HF 1 x 1 mL Octyl PuraBead® HF

HIC selection kit (5 mL columns)

1 x 5 mL Phenyl PuraBead® HF 1 x 5 mL Butyl PuraBead® HF 1 x 5 mL Hexyl PuraBead® HF 1 x 5 mL Octyl PuraBead® HF

6614

6615

Single Columns Product code

Description

6710

Phenyl PuraBead HF Column

1 x 1 mL

Robocolumns

6712

Octyl PuraBead® HF Column

1 x 1 mL

Product code

Description

1 x 1 mL

4520-PC3408

Phenyl PuraBead HF, 200 µL

1 row of 8

Hexyl PuraBead® HF, 200 µL

1 row of 8

6734

Pack size ®

Butyl PuraBead® HF Column

Pack size ®

6736

Hexyl PuraBead® HF Column

1 x 1 mL

4520-PC3426

6711

Phenyl PuraBead® HF Column

1 x 5 mL

4520-PC3455

Octyl PuraBead® HF, 200 µL

1 row of 8

6713

Octyl PuraBead® HF Column

1 x 5 mL

4520-PC3416

Butyl PuraBead® HF, 200 µL

1 row of 8

6735

Butyl PuraBead® HF Column

1 x 5 mL

6737

Hexyl PuraBead HF Column

1 x 5 mL

®

+44 (0) 1223 433 800 | astreabioseparations.com sales@astrea-bio.com | techsupport@astrea-bio.com | quality@astrea-bio.com Global bases in North America, Canada and Cambridge UK HQ: Horizon Park, Barton Road, Comberton, Cambridge, CB23 7AJ, UK

HIC Purabead® HF - V2 This product is covered by or for use under one or more patents: astreabioseparations.com/patents All trademarks, trade names, trade dress, product names and logos appearing on this document are the property of Astrea UK Services Limited or their respective owners.

RoboColumns are packed by, and RoboColumn® is a trademark of Repligen GmbH. Copyright © 2023 Astrea Bioseparations Ltd. All rights reserved.