Isolation Kit | Installation & Instruction Guide (Rev C | BP40011A)

Chromatography Column Column Isolation Kit Installation and Instruction Guide

DELTA PRECISION LTD

Chromatography Column

Column Isolation Kit Installation and Instruction Guide

Revision History Revision A B C

Comments Initial release Address updated Update to reflect Evolve® Trademark Status

Date 30/01/2016 21/06/2018 12/07/2021

Evolve® is a registered trademark of Delta Precision Ltd. Evolve® is a registered trademark in the EU, UK and US.

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Column Isolation Kit Kit contents and Operating Instructions During normal operation it can be necessary to isolate the column from the process flow.

This can be during connection or disconnection to the pumping system or to ensure that no air is in the pipework or during cleaning procedures.

It is possible to mount the isolation valve on the top or side of the column. Kits AACIK1 and AACIK3 are sized for Evolve® 70 and Evolve 100 columns. Whereas kits AACIK2 and AACIK4 are sized for Evolve® 140, 200 and 250 columns.

Column isolation kit

1 1 2 2 3 3 3 3 4 4 5 6 6 7 8

X

AACIK4

QTY 1 1 1 1 1 1 1 1 1 1 2 5 5 5 1

AACIK3

DESCRIPTION Flexible hoses length 1.85m 3mm od Flexible hoses length 1.85m 6mm od Flexible hoses length 1.2m 3mm od Flexible hoses length 1.2m 6mm od Accessory Support Bracket (Top, small) Accessory Support Bracket (Top, large) Accessory Support Bracket (Side, small) Accessory Support Bracket (Side, large) 4 port 2 way valve small 4 port 2 way valve large Blanking Cap Ladish Seal 6mm id Ladish Seal 3mm id Clamps Data Sheet

AACIK2

PRODUCT NUMBER

AACIK1

Number On Figures 1 &2

X X

X

X X

X

X

X X X X X X X X X

X X X X X X

X X X X

X X X X X

All materials in contact with the process stream have been selected for their suitability for use in equipment utilised in Biopharmaceutical applications and so either conform to relevant sections of FDA code of Federal Regulations vol. 21 170-199 and/or passed USP class VI test for in vivo toxicity.

Wetted Components

Material

4 port 2 way valve small 4 port 2 way valve large Ladish TC Seals Flexible hoses (3mm id and 6mm id) all lengths Blanking Cap Clamps

PVDF valve Body/PTFE Ball/EPDM seals PVDF valve Body/PTFE Ball/EPDM seals Santoprene® Braided PVC hose/ Hytrel ends Polypropylene Nylon 1

Non Wetted Components Bracket Small & Large (Top mounting) Bracket Small & Large (Side mounting) Bolts/Rod

Material Acetal Acetal Stainless steel type 316

Assembly instructions are described below, tools required are provided with the Evolve Column.

Assembly of Column Isolation Kit (top mounted)

Figure 1 Contents of column isolation kit for top mounting AACIK1 & AACIK2 Assembly of Accessory Support Bracket Before assembling the column isolation kit, it is necessary to assemble the accessory support bracket.

Follow the instructions below:

Support strap

Rod

Grub Screw 1

Support Bracket

Grub Screw 2 Figure 2 Components of Accessory Support Bracket 2

Collar

1.

2.

3.

4.

5.

6.

Remove the flow tube adaptor Remove seal adjuster nut and fit the new collar Locate the rod into the support bracket Fit the support bracket to the seal actuation nut but do not tighten grub screw 1.

Replace the seal actuation nut Slide the support strap over the rod and the flowtube. DO NOT TIGHTEN this grub screw at this stage.

7. Re-fit the flow tube adaptor 8. Set column to the required height and tighten Grub screw 1 and grub screw 2. Fit required accessories to the flowtube adaptor.

NOTE Both grub screws must be undone before the turning the seal actuation nut Column Online Column Out

Process In

COLUMN

Column Isolate Column Out

Process In

COLUMN

Figure 3 Flow diagram of Kit contents (top mounted valve)

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Assembly of Column Isolation Kit (side mounted)

Figure 4 Contents of column isolation kit for top mounting AACIK3 & AACIK4 The side support bracket is fitted around a tie bar on the column and to a vertically orientated port of the 4-Port valve. Ensure that the bolts are tighten so the valve is secure.

Column Online

Process In

COLUMN Column Out

Figure 5a Flow diagram of Kit contents (side mounted valve) for Column Online

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Column Isolate Process In

COLUMN

Flow Out

Figure 5b Flow diagram of Kit contents (side mounted valve) for Column Isolated

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Contact Us To view our Evolve® Column User guides please visit: www.astreabioseparations.com/resources/evolve -column-user-guides For further enquires please contact: sales@astrea-bio.com

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